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1. 发明授权

US4752638A Synthesis and use of polymers containing integral binding-pair members 失效
标题翻译：含有整体结合对成员的聚合物的合成和使用
公开(公告)号：US4752638A
公开(公告)日：1988-06-21
申请号：US880122
申请日：1986-06-30
申请人： Robert C. Nowinski , Allan S. Hoffman , Raymond L. Houghton , John H. Priest , Nobuo Monji
发明人： Robert C. Nowinski , Allan S. Hoffman , Raymond L. Houghton , John H. Priest , Nobuo Monji
IPC分类号： C08F299/02 , G01N33/545 , C08L89/00 , C08H1/00
CPC分类号： C08F299/022 , G01N33/545 , G01N2650/00 , Y10S436/827 , Y10S530/817
摘要： Conjugate monomers, polymers, and methods for the de novo synthesis of the polymers are provided. Conjugate organic monomers contain binding-pair members which upon polymerization become integrally associated with the resultant polymer. Specifically, antigens, antibodies, receptors, and ligands may be bound to organic monomers either directly by chemical reaction or indirectly by chemical spacer arms, and these conjugates may be polymerized or copolymerized with nonderivatized monomers to form polymers containing variable amounts of the binding-pair members. Such conjugate monomers and polymers find a wide variety of uses in binding to their binding-pair-member cognate which include selective removal of complementary binding-pair members from solution as well as in immunoassay procedures and in immunization regimes.
摘要翻译：提供了用于聚合物的从头合成的共轭单体，聚合物和方法。共轭有机单体含有结合对成员，其在聚合时与所得聚合物整体相关。具体地，抗原，抗体，受体和配体可以直接通过化学反应或通过化学间隔臂间接地结合到有机单体上，并且这些缀合物可以与非稀释的单体聚合或共聚以形成含有可变量的结合对的聚合物会员这种缀合物单体和聚合物在结合它们的结合对成员同源物中发现了各种各样的用途，其包括从溶液以及免疫测定方法和免疫方案中选择性除去互补结合对成员。

2. 发明授权

US4780409A Thermally induced phase separation immunoassay 失效
标题翻译：热诱导相分离免疫测定
公开(公告)号：US4780409A
公开(公告)日：1988-10-25
申请号：US854831
申请日：1986-04-28
申请人： Nobuo Monji , Allan S. Hoffman , John H. Priest , Raymond L. Houghton
发明人： Nobuo Monji , Allan S. Hoffman , John H. Priest , Raymond L. Houghton
IPC分类号： G01N33/543 , G01N33/531 , G01N33/537 , G01N33/545 , G01N33/569
CPC分类号： G01N33/545 , G01N33/531 , G01N33/5375 , G01N33/56927 , Y10S435/971 , Y10S435/973 , Y10S436/824 , Y10S436/827
摘要： An immunoassay in which a thermally induced phase separation is used to effect the separation of specifically bound reactants from free reactants is disclosed. A first reactant is conjugated to a temperature-sensitive polymer to form a polymer/reactant conjugate, and a second reactant is conjugated to a reporter to form a reporter/reactant conjugate. The polymer/reactant, reporter/reactant, and biological fluid samples suspected of containing the analyte are admixed in solution at a temperature other than that at which the polymer will precipitate. Specific binding is allowed to occur, thereby forming a ternary complex. The salt concentration of the adjusted solution is then adjusted to a concentration sufficient to cause the complex to precipitate from the solution, the amount of reporter activity in the precipitated complex or in the solution measured and the presence and/or concentration of the analyte therefrom determined. Alternatively, the first reactant may be conjugated to a monomer and subsequently copolymerized with additional monomers to yield a temperature-sensitive copolymer. Multiple analyses may also be performed on a single sample by choosing a variety of polymers, each polymer having a different specific binding partner conjugated thereto and a different critical solution temperature. By altering the temperature and/or the salt concentration of the solution incrementally, the reporter associated with each of the complexes precipitated with each temperature or concentration increment may be measured, and the presence and/or concentration of each of the analytes determined.
摘要翻译：公开了一种免疫测定法，其中使用热诱导相分离来实现特异性结合的反应物与游离反应物的分离。将第一反应物与温度敏感的聚合物缀合以形成聚合物/反应物缀合物，并且将第二反应物与报道分子缀合以形成报告物/反应物缀合物。怀疑含有分析物的聚合物/反应物，报告物/反应物和生物液体样品在除聚合物沉淀之外的温度下在溶液中混合。允许发生特异性结合，从而形成三元复合物。然后将调整后的溶液的盐浓度调整至足以引起络合物从溶液中沉淀出的浓度，测定沉淀的络合物或溶液中的报告物活性的量以及测定的分析物的存在和/或浓度。或者，第一反应物可以与单体缀合，随后与另外的单体共聚以产生温度敏感的共聚物。也可以通过选择各种聚合物对单个样品进行多重分析，每种聚合物具有与其缀合的不同的特异性结合对象和不同的临界溶解温度。通过逐渐改变溶液的温度和/或盐浓度，可以测量与每个温度或浓度增量沉淀的每个络合物相关的报告物，并测定每种分析物的存在和/或浓度。

3. 发明授权

US4912032A Methods for selectively reacting ligands immobilized within a temperature-sensitive polymer gel 失效
标题翻译：选择性地使固定在温度敏感的聚合物凝胶中的配体反应的方法
公开(公告)号：US4912032A
公开(公告)日：1990-03-27
申请号：US948377
申请日：1986-12-31
申请人： Allan S. Hoffman , Nobuo Monji
发明人： Allan S. Hoffman , Nobuo Monji
IPC分类号： A61K9/20 , B01D15/00 , C02F9/00
CPC分类号： C02F9/005 , A61K9/2027 , B01D15/00 , B01D15/34 , B01D15/3804 , B01D15/3876 , Y10S436/824
摘要： Methods for delivering substances into, removing substances from, or reacting substances with a selected environment utilizing polymer gels or coatings characterized by a critical solution temperature (CST) are disclosed. The CST as well as the pore structure, pore size, pore distribution, and absorbing capacity of the gel may be selectively controlled. The substances may be physically or chemically immobilized within the polymer gels. In addition, a method for altering the surface wettability of CST polymers is also disclosed.
摘要翻译：公开了使用聚合物凝胶或以临界溶解温度（CST）为特征的涂层将物质输送，从物质中除去或与物质反应的方法。可以选择性地控制CST以及凝胶的孔结构，孔径，孔分布和吸收能力。物质可以在聚合物凝胶内物理或化学固定。此外，还公开了改变CST聚合物的表面润湿性的方法。

4. 发明授权

US07144697B1 Synthetic antigen for the detection of antibodies immunoreactive with HIV virus 有权
标题翻译：用于检测与HIV病毒免疫反应的抗体的合成抗原
公开(公告)号：US07144697B1
公开(公告)日：2006-12-05
申请号：US09515014
申请日：2000-02-29
申请人： Patrick F. Coleman , Peter Chong-Dug Su , Nobuo Monji , Carol-Ann Cole
发明人： Patrick F. Coleman , Peter Chong-Dug Su , Nobuo Monji , Carol-Ann Cole
IPC分类号： C12Q1/70
CPC分类号： C07K14/005 , C12N2740/16222 , G01N33/56988 , G01N2469/20
摘要： Novel polypeptides are provided having substantially the same sequence as immunologically significant fragments of AIDS-related viruses. The polypeptides can be used as reagents in the determination of exposure of a human host to the virus. Of particular interest is the use of polypeptides in screening blood products.
摘要翻译：提供了与AIDS相关病毒的免疫学显着片段基本相同的序列的新型多肽。多肽可用作测定人宿主对病毒暴露的试剂。特别感兴趣的是使用多肽筛选血液制品。

5. 发明授权

US5439792A Cysteine thiol-protected peptides for use in immunoassays 失效
标题翻译：用于免疫测定的半胱氨酸硫醇保护的肽
公开(公告)号：US5439792A
公开(公告)日：1995-08-08
申请号：US140696
申请日：1993-10-21
申请人： James Blake , Carol-Ann Cole , Patrick F. Coleman , Nobuo Monji , John P. Montana
发明人： James Blake , Carol-Ann Cole , Patrick F. Coleman , Nobuo Monji , John P. Montana
IPC分类号： C07K1/107 , C07K14/16 , C07K17/02 , C12Q1/00
CPC分类号： C07K14/005 , C07K1/1077 , C07K17/02 , C12N2740/16022 , C12N2740/16122 , Y10S435/974
摘要： Peptides immunoreactive with antibodies to native proteins, and which have at least two cysteine residues that contribute to mimicking an epitope of the protein, are prepared with the cysteine thiol groups protected. When deprotected, the peptides have enhanced immunoreactivity. The peptides are particularly useful for detecting antibodies or antigens associated with retroviruses, including the clinically important lymphotropic retroviruses HIV-1, HIV-2, HTLV-I, and HTLV-II.
摘要翻译：与保护的半胱氨酸硫醇基团一起制备与天然蛋白质抗体免疫反应的肽，并且其具有有助于模拟蛋白质表位的至少两个半胱氨酸残基。当脱保护时，肽具有增强的免疫反应性。这些肽特别可用于检测与逆转录病毒相关的抗体或抗原，包括临床上重要的淋巴细胞逆转录病毒HIV-1，HIV-2，HTLV-1和HTLV-II。

6. 发明授权

US5206178A Membrane affinity concentration immunoassay 失效
标题翻译：膜亲和力浓度免疫测定
公开(公告)号：US5206178A
公开(公告)日：1993-04-27
申请号：US663193
申请日：1991-02-28
申请人： Nobuo Monji , Carol-Ann Cole
发明人： Nobuo Monji , Carol-Ann Cole
IPC分类号： G01N33/538 , G01N33/543 , G01N33/545
CPC分类号： G01N33/545 , G01N33/538 , G01N33/54306 , Y10S435/971 , Y10S436/81
摘要： Methods for determining the presence and/or concentration of an analyte in a biological fluid sample are disclosed. The methods generally include admixing in solution certain polymer/reactant and reporter/reactant conjugates along with the biological fluid sample suspected of containing the analyte, thereby forming ternary complexes. The separation of the complexes from the reaction mixture is achieved through the affinity of certain selected polymer compositions for various solid phases. Upon separation, the amount of reporter activity in the solution may be measured, and therefrom the presence and/or concentration of the analyte determined. Multiple analyses on a biological fluid sample suspected of containing one or more analytes may also be performed, using either a variety of different reporters or selected polymers having varied affinity for the solid phase.
摘要翻译：公开了用于确定生物流体样品中分析物的存在和/或浓度的方法。方法通常包括在溶液中混合某些聚合物/反应物和报告物/反应物共轭物以及怀疑含有分析物的生物流体样品，从而形成三元复合物。复合物与反应混合物的分离通过某些所选聚合物组合物对于各种固相的亲和力来实现。在分离时，可以测量溶液中的报告物活性的量，由此测定分析物的存在和/或浓度。怀疑含有一种或多种分析物的生物流体样品的多个分析也可以使用各种不同的报告物或对固相具有不同亲和力的所选聚合物进行。

7. 发明授权

US4323647A Steric hindrance enzyme immunoassay 失效
标题翻译：空肠阻抗酶免疫测定
公开(公告)号：US4323647A
公开(公告)日：1982-04-06
申请号：US197063
申请日：1980-10-15
申请人： Nobuo Monji , Albert Castro
发明人： Nobuo Monji , Albert Castro
IPC分类号： G01N33/538 , G01N33/542
CPC分类号： G01N33/538 , G01N33/542 , Y10S435/81 , Y10S435/964 , Y10S435/971 , Y10S435/975
摘要： A novel separation technique is described that is particularly useful for effecting separations in enzyme immunoassay procedures. A mixture, in an aqueous liquid vehicle, of (1), ligand-enzyme conjugate, and of (2), the conjugate bound through its ligand moiety to a receptor, is brought into contact with an insoluble, immobilized pseudo-substrate material, to which the enzyme normally binds. Free conjugate binds and becomes insoluble. Bound conjugate remains in the liquid phase. The ligand may be an antigen and the receptor, the antibody to the antigen.This separation technique makes feasible several sensitive immunoassay procedures. The material to be assayed may be, for example, rubella virus; hepatitis B surface antigen; gonorrhea antigen; the antibody to any of the foregoing; a general antibody, i.e., an immunoglobulin; a hormone such as choriomammotropin; a steroid, hapten, or the like.
摘要翻译：描述了一种新颖的分离技术，其特别可用于实现酶免疫测定方法中的分离。在水性液体载体中，（1）配体 - 酶偶联物和（2）的混合物，通过其配体部分与受体结合的缀合物与不溶性固定的假底物材料接触，酶通常结合到其上。游离共轭物结合并变得不溶。结合的结合物保留在液相中。配体可以是抗原和受体，即抗原的抗体。这种分离技术使几种敏感的免疫测定方法成为可行。要测定的材料可以是例如风疹病毒; 乙型肝炎表面抗原; 淋病抗原针对上述任何一种的抗体; 一般抗体，即免疫球蛋白; 一种激素如脉络膜激素; 类固醇，半抗原等。

8. 发明申请

US20130065249A1 SIGNAL AMPLIFICATION FOR IMMUNOASSAYS BY USE OF AVIDIN-BIOTIN LINKAGES 审中-公开
标题翻译：通过使用AVIDIN-BIOTIN链接免疫免疫的信号放大
公开(公告)号：US20130065249A1
公开(公告)日：2013-03-14
申请号：US13411846
申请日：2012-03-05
申请人： WOEI TAN , Nobuo Monji , Vinita Gupta , Candice Reyes
发明人： WOEI TAN , Nobuo Monji , Vinita Gupta , Candice Reyes
IPC分类号： G01N21/64
CPC分类号： G01N33/54306 , G01N33/542
摘要： In sandwich-type immunoassays that capture a protein analyte between a capture antibody, typically bound to a solid phase, and a detection antibody that is coupled to a reporter group, the number of reporter groups associated with each molecule of analyte is increased by a variety of methods that utilize avidin-biotin-type binding in conjunction with such features as immunological binding to the reporter group on the detection antibody or multiple biotin-avidin-type binding sites.
摘要翻译：在通常与固相结合的捕获抗体和与报告基团连接的检测抗体之间捕获蛋白质分析物的夹心型免疫测定中，与每个分析物分子相关联的报告基团数量增加了多种利用抗生物素蛋白 - 生物素型结合与检测抗体或多个生物素 - 抗生物素蛋白型结合位点等免疫学结合到报告基团的特征的方法。

9. 发明授权

US5206136A Rapid membrane affinity concentration assays 失效
标题翻译：快速膜亲和力浓度测定
公开(公告)号：US5206136A
公开(公告)日：1993-04-27
申请号：US925311
申请日：1992-07-31
申请人： Nobuo Monji , Carol-Ann Cole
发明人： Nobuo Monji , Carol-Ann Cole
IPC分类号： G01N33/538 , G01N33/543 , G01N33/545
CPC分类号： G01N33/545 , G01N33/538 , G01N33/54306 , Y10S435/803 , Y10S435/971 , Y10S435/974 , Y10S436/81
摘要： Rapid assays for analytes of interest in a fluid sample utilize a first conjugate of a labelled reactant that specifically binds to the analyte, and a second conjugate that binds to the analyte coupled to a polymer that has an affinity for a selected solid phase. The reaction components are incubated briefly, then contacted with the selected solid phase and the labelled components determined. Optional wash steps provide for enhanced sensitivity and specificity. When the analyte of interest is an antibody to HIV, the first reactant may be a synthetic, recombinant or native HIV antigen, and the second reactant may be protein A or an anti-immunoglobulin.
摘要翻译：对流体样品中感兴趣的分析物的快速测定利用特异性结合分析物的标记反应物的第一缀合物，以及结合与聚合物偶联的分析物的第二偶联物，所述分析物与选定的固相具有亲和性。将反应组分短暂孵育，然后与选定的固相接触并测定标记的组分。可选的洗涤步骤提供增强的灵敏度和特异性。当感兴趣的分析物是针对HIV的抗体时，第一反应物可以是合成的，重组的或天然的HIV抗原，第二反应物可以是蛋白A或抗免疫球蛋白。

10. 发明授权

US4474758A Haemophilus influenzae type b and pertussis outer membrane component combined vaccine 失效
标题翻译：流感嗜血杆菌b型和百日咳外膜组分联合疫苗
公开(公告)号：US4474758A
公开(公告)日：1984-10-02
申请号：US323523
申请日：1981-11-19
申请人： Joseph S. C. Kuo , Nobuo Monji
发明人： Joseph S. C. Kuo , Nobuo Monji
IPC分类号： A61K39/095 , A61K39/10 , A61K39/102 , A61K39/106 , A61K39/116
CPC分类号： A61K39/102 , A61K2039/6068 , Y10S424/831
摘要： A combined vaccine for eliciting polyribosyl ribitol phosphate (PRP) antibody formations in warm-blooded animals has been invented. The combined vaccine comprises the capsular polysaccharide PRP isolated and purified from Haemophilus influenzae type b and antigens isolated and purified from an outer membrane component of Bordetella pertussis.A method for inducing active immunization in warm-blooded animals against systemic infection caused by the pathogen H. influenzae type b has also been invented. The method comprises administering an immunogenic amount of a combined vaccine comprising the capsular polysaccharide PRP isolated and purified from H. influenzae type b and antigens isolated and purified from an outer membrane component of B. pertussis.
摘要翻译：已经发明了用于在温血动物中引发多核糖核糖醇磷酸（PRP）抗体结构的组合疫苗。联合疫苗包括从流感嗜血杆菌B型分离和纯化的荚膜多糖PRP和从百日咳博德特氏菌的外膜组分分离和纯化的抗原。还发现了一种在温血动物中诱导由b型流感嗜血杆菌引起的全身感染的主动免疫的方法。该方法包括施用免疫原性量的组合疫苗，其包含从流感嗜血杆菌b型分离和纯化的荚膜多糖PRP和从百日咳百日咳杆菌的外膜组分分离和纯化的抗原。

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