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1. 发明授权

US4752638A Synthesis and use of polymers containing integral binding-pair members 失效
标题翻译：含有整体结合对成员的聚合物的合成和使用
公开(公告)号：US4752638A
公开(公告)日：1988-06-21
申请号：US880122
申请日：1986-06-30
申请人： Robert C. Nowinski , Allan S. Hoffman , Raymond L. Houghton , John H. Priest , Nobuo Monji
发明人： Robert C. Nowinski , Allan S. Hoffman , Raymond L. Houghton , John H. Priest , Nobuo Monji
IPC分类号： C08F299/02 , G01N33/545 , C08L89/00 , C08H1/00
CPC分类号： C08F299/022 , G01N33/545 , G01N2650/00 , Y10S436/827 , Y10S530/817
摘要： Conjugate monomers, polymers, and methods for the de novo synthesis of the polymers are provided. Conjugate organic monomers contain binding-pair members which upon polymerization become integrally associated with the resultant polymer. Specifically, antigens, antibodies, receptors, and ligands may be bound to organic monomers either directly by chemical reaction or indirectly by chemical spacer arms, and these conjugates may be polymerized or copolymerized with nonderivatized monomers to form polymers containing variable amounts of the binding-pair members. Such conjugate monomers and polymers find a wide variety of uses in binding to their binding-pair-member cognate which include selective removal of complementary binding-pair members from solution as well as in immunoassay procedures and in immunization regimes.
摘要翻译：提供了用于聚合物的从头合成的共轭单体，聚合物和方法。共轭有机单体含有结合对成员，其在聚合时与所得聚合物整体相关。具体地，抗原，抗体，受体和配体可以直接通过化学反应或通过化学间隔臂间接地结合到有机单体上，并且这些缀合物可以与非稀释的单体聚合或共聚以形成含有可变量的结合对的聚合物会员这种缀合物单体和聚合物在结合它们的结合对成员同源物中发现了各种各样的用途，其包括从溶液以及免疫测定方法和免疫方案中选择性除去互补结合对成员。

2. 发明授权

US4780409A Thermally induced phase separation immunoassay 失效
标题翻译：热诱导相分离免疫测定
公开(公告)号：US4780409A
公开(公告)日：1988-10-25
申请号：US854831
申请日：1986-04-28
申请人： Nobuo Monji , Allan S. Hoffman , John H. Priest , Raymond L. Houghton
发明人： Nobuo Monji , Allan S. Hoffman , John H. Priest , Raymond L. Houghton
IPC分类号： G01N33/543 , G01N33/531 , G01N33/537 , G01N33/545 , G01N33/569
CPC分类号： G01N33/545 , G01N33/531 , G01N33/5375 , G01N33/56927 , Y10S435/971 , Y10S435/973 , Y10S436/824 , Y10S436/827
摘要： An immunoassay in which a thermally induced phase separation is used to effect the separation of specifically bound reactants from free reactants is disclosed. A first reactant is conjugated to a temperature-sensitive polymer to form a polymer/reactant conjugate, and a second reactant is conjugated to a reporter to form a reporter/reactant conjugate. The polymer/reactant, reporter/reactant, and biological fluid samples suspected of containing the analyte are admixed in solution at a temperature other than that at which the polymer will precipitate. Specific binding is allowed to occur, thereby forming a ternary complex. The salt concentration of the adjusted solution is then adjusted to a concentration sufficient to cause the complex to precipitate from the solution, the amount of reporter activity in the precipitated complex or in the solution measured and the presence and/or concentration of the analyte therefrom determined. Alternatively, the first reactant may be conjugated to a monomer and subsequently copolymerized with additional monomers to yield a temperature-sensitive copolymer. Multiple analyses may also be performed on a single sample by choosing a variety of polymers, each polymer having a different specific binding partner conjugated thereto and a different critical solution temperature. By altering the temperature and/or the salt concentration of the solution incrementally, the reporter associated with each of the complexes precipitated with each temperature or concentration increment may be measured, and the presence and/or concentration of each of the analytes determined.
摘要翻译：公开了一种免疫测定法，其中使用热诱导相分离来实现特异性结合的反应物与游离反应物的分离。将第一反应物与温度敏感的聚合物缀合以形成聚合物/反应物缀合物，并且将第二反应物与报道分子缀合以形成报告物/反应物缀合物。怀疑含有分析物的聚合物/反应物，报告物/反应物和生物液体样品在除聚合物沉淀之外的温度下在溶液中混合。允许发生特异性结合，从而形成三元复合物。然后将调整后的溶液的盐浓度调整至足以引起络合物从溶液中沉淀出的浓度，测定沉淀的络合物或溶液中的报告物活性的量以及测定的分析物的存在和/或浓度。或者，第一反应物可以与单体缀合，随后与另外的单体共聚以产生温度敏感的共聚物。也可以通过选择各种聚合物对单个样品进行多重分析，每种聚合物具有与其缀合的不同的特异性结合对象和不同的临界溶解温度。通过逐渐改变溶液的温度和/或盐浓度，可以测量与每个温度或浓度增量沉淀的每个络合物相关的报告物，并测定每种分析物的存在和/或浓度。

3. 发明授权

US4749647A Polymerization-induced separation assay using recognition pairs 失效
标题翻译：使用识别对的聚合诱导分离测定
公开(公告)号：US4749647A
公开(公告)日：1988-06-07
申请号：US623838
申请日：1984-06-22
申请人： Elaine K. Thomas , Dennis E. Schwartz , John H. Priest , Robert C. Nowinski , Allan S. Hoffman
发明人： Elaine K. Thomas , Dennis E. Schwartz , John H. Priest , Robert C. Nowinski , Allan S. Hoffman
IPC分类号： C12Q1/68 , C12Q1/70 , G01N33/537
CPC分类号： G01N33/5375 , C12Q1/68 , C12Q1/682 , C12Q1/701 , Y10S436/827 , Y10S525/904
摘要： Methods and compounds are disclosed for determining the presence, amount of, or association between substances of interest in samples suspected of containing same. The methods are based on the polymerization-induced separation of specifically-bound, reporter-labeled recognition reactants from free, reporter-labeled recognition reactants. The methods described are applicable to any substance for which suitable recognition reactants exist or can be made and are not limited by considerations such as chemical composition or molecular size.
摘要翻译：公开了用于确定怀疑含有其的样品中感兴趣的物质的存在，量或关联的方法和化合物。该方法基于聚合诱导的分离来自游离的报道分子标记的识别反应物的特异结合的报道分子标记的识别反应物。所描述的方法适用于存在或可以制备合适的识别反应物的任何物质，并且不受诸如化学组成或分子大小等考虑的限制。

4. 发明授权

US4843010A Polymerization-induced separation immunoassays 失效
标题翻译：聚合诱导分离免疫测定
公开(公告)号：US4843010A
公开(公告)日：1989-06-27
申请号：US574558
申请日：1984-01-27
申请人： Robert C. Nowinski , Allan S. Hoffman
发明人： Robert C. Nowinski , Allan S. Hoffman
IPC分类号： C08F290/14 , G01N33/545
CPC分类号： G01N33/545 , C08F290/14 , G01N2650/00 , Y10S525/904
摘要： Immunoassay methods and compositions are disclosed for the detection of analytes in fluid samples. The disclosure provides conjugates of analytes or reactants with polymerizable organic monomers. Specific binding reactions between reactants are detected by means of resporter/reactant conjugates. Free and specifically-bound reporter/reactant conjugates are separated by a polymerization reaction which renders the polymerized monomers insolule.
摘要翻译：公开了用于检测流体样品中分析物的免疫测定方法和组合物。本公开提供分析物或反应物与可聚合有机单体的缀合物。反应物之间的特异性结合反应可以通过还原剂/反应物共轭物进行检测。游离和特异结合的报告物/反应物共轭物通过聚合反应分离，使聚合的单体不规则。

5. 发明授权

US4711840A Polymerization-induced separation immunoassays 失效
标题翻译：聚合诱导分离免疫测定
公开(公告)号：US4711840A
公开(公告)日：1987-12-08
申请号：US668247
申请日：1984-11-07
申请人： Robert C. Nowinski , Allan S. Hoffman
发明人： Robert C. Nowinski , Allan S. Hoffman
IPC分类号： C08F299/02 , G01N33/545 , G01N33/537 , C12Q1/68
CPC分类号： G01N33/545 , C08F299/022 , Y10S436/80 , Y10S525/904
摘要： Immunoassay methods and compositions are disclosed for the detection of one or more analytes in fluid samples. The disclosure provides conjugates of analytes or reactants with polymerizable organic monomers. Specific binding reactions between reactants are detected by means of reporter/reactant conjugates. Free and specifically-bound reporter/reactant conjugates are separated by a polymerization reaction which renders the polymerized monomers insoluble.
摘要翻译：公开了用于检测流体样品中一种或多种分析物的免疫测定方法和组合物。本公开提供分析物或反应物与可聚合有机单体的缀合物。通过报告物/反应物共轭物检测反应物之间的特异性结合反应。通过使聚合单体不溶的聚合反应分离游离和特异结合的报告物/反应物共轭物。

6. 发明授权

US4609707A Synthesis of polymers containing integral antibodies 失效
标题翻译：含有整体抗体的聚合物的合成
公开(公告)号：US4609707A
公开(公告)日：1986-09-02
申请号：US668248
申请日：1984-11-07
申请人： Robert C. Nowinski , Allan S. Hoffman
发明人： Robert C. Nowinski , Allan S. Hoffman
IPC分类号： C08F290/14 , G01N33/545 , C08L89/00 , C08H1/00
CPC分类号： C08F290/14 , G01N33/545 , G01N2650/00 , Y10S436/827 , Y10S530/817
摘要： A method is disclosed for the de novo synthesis of antibody-containing polymers and the preparation of a class of polymerizable compounds used in the synthesis of such antibody-containing polymers. Antibody-containing polymers formed from monomer/antibody conjugates and nonderivatized polymerizable compounds can be varied in formation of the polymer to provide control of (a) molecular spacing, steric accessibility and the number of antibody molecules that are integrally incorporated into the polymer backbone, and (b) the chemical and physical structure of the polymer itself, thus enabling specific tailoring of antibody-containing polymers for particular end-use application. Also disclosed is a method for the selective removal of a compound from a solution or suspension thereof using monomer/receptor conjugates where the compound has the capacity to bind to the receptor in the conjugate. The bound compound is removed by polymerization - induced separation of the monomer/receptor--compound complex from solution.
摘要翻译：公开了用于从含合成抗体的聚合物和制备用于合成这种含抗体聚合物的一类可聚合化合物的方法。由单体/抗体缀合物和非稀释的可聚合化合物形成的含抗体的聚合物可以在形成聚合物时变化，以提供（a）分子间距，空间可及性和整体并入聚合物主链中的抗体分子的数量的控制，以及（b）聚合物本身的化学和物理结构，从而能够特异性地定制含抗体的聚合物用于特定的最终应用。还公开了使用单体/受体共轭物从其溶液或悬浮液中选择性除去化合物的方法，其中化合物具有结合缀合物中受体的能力。通过聚合诱导分离单体/受体复合物从溶液中除去结合的化合物。

7. 发明授权

US4511478A Polymerizable compounds and methods for preparing synthetic polymers that integrally contain polypeptides 失效
标题翻译：可聚合的化合物和用于制备整体含有多肽的合成聚合物的方法
公开(公告)号：US4511478A
公开(公告)日：1985-04-16
申请号：US550929
申请日：1983-11-10
申请人： Robert C. Nowinski , Allan S. Hoffman
发明人： Robert C. Nowinski , Allan S. Hoffman
IPC分类号： C08F290/14 , G01N33/545 , C08L89/00 , C08H1/00
CPC分类号： C08F290/14 , G01N33/545 , G01N2650/00 , Y10S436/827
摘要： A method is disclosed for the de novo synthesis of polypeptide-containing polymers. This disclosure also includes a description of, and a method for the preparation of, a class of polymerizable compounds used in the synthesis of polypeptide-containing polymers. These polymerizable compounds are chemical conjugates prepared by covalent linkage of polymerizable organic monomers with specific polypeptides. Soluble monomer/polypeptide conjugates can be polymerized in solution with additional nonderivatized organic monomers to form desired polypeptide-containing polymers. The amount and composition of monomer and monomer/polypeptide conjugates can be varied in order to provide control of (a) molecular spacing, steric accessibility, and number of polypeptide molecules that are integrally incorporated into the polymer backbone, and (b) the chemical and physical structure of the polymer itself. This enables the specific tailoring of polypeptide-containing polymers for particular end-use applications.
摘要翻译：公开了用于从头合成含多肽的聚合物的方法。本公开还包括用于合成含多肽的聚合物中使用的一类可聚合化合物的描述和制备方法。这些可聚合化合物是通过可聚合有机单体与特定多肽的共价连接制备的化学共轭物。可溶性单体/多肽缀合物可以在溶液中与另外的非衍生化有机单体聚合以形成所需的含多肽的聚合物。可以改变单体和单体/多肽缀合物的量和组成，以便提供（a）分子间距，空间可及性和整体并入到聚合物主链中的多肽分子的数量的控制，和（b）化学和/ 聚合物本身的物理结构。这使得能够特定定制含多肽的聚合物用于特定的最终用途应用。

8. 发明授权

US4912032A Methods for selectively reacting ligands immobilized within a temperature-sensitive polymer gel 失效
标题翻译：选择性地使固定在温度敏感的聚合物凝胶中的配体反应的方法
公开(公告)号：US4912032A
公开(公告)日：1990-03-27
申请号：US948377
申请日：1986-12-31
申请人： Allan S. Hoffman , Nobuo Monji
发明人： Allan S. Hoffman , Nobuo Monji
IPC分类号： A61K9/20 , B01D15/00 , C02F9/00
CPC分类号： C02F9/005 , A61K9/2027 , B01D15/00 , B01D15/34 , B01D15/3804 , B01D15/3876 , Y10S436/824
摘要： Methods for delivering substances into, removing substances from, or reacting substances with a selected environment utilizing polymer gels or coatings characterized by a critical solution temperature (CST) are disclosed. The CST as well as the pore structure, pore size, pore distribution, and absorbing capacity of the gel may be selectively controlled. The substances may be physically or chemically immobilized within the polymer gels. In addition, a method for altering the surface wettability of CST polymers is also disclosed.
摘要翻译：公开了使用聚合物凝胶或以临界溶解温度（CST）为特征的涂层将物质输送，从物质中除去或与物质反应的方法。可以选择性地控制CST以及凝胶的孔结构，孔径，孔分布和吸收能力。物质可以在聚合物凝胶内物理或化学固定。此外，还公开了改变CST聚合物的表面润湿性的方法。

9. 发明授权

US09476063B2 Diblock copolymers and polynucleotide complexes thereof for delivery into cells 有权
标题翻译：二嵌段共聚物和其多核苷酸复合物用于递送到细胞中
公开(公告)号：US09476063B2
公开(公告)日：2016-10-25
申请号：US12992517
申请日：2009-05-13
申请人： Patrick S. Stayton , Allan S. Hoffman , Anthony J. Convertine , Danielle Benoit , Craig L. Duvall , Paul H. Johnson , Anna S. Gall
发明人： Patrick S. Stayton , Allan S. Hoffman , Anthony J. Convertine , Danielle Benoit , Craig L. Duvall , Paul H. Johnson , Anna S. Gall
IPC分类号： C08F293/00 , C12N5/071 , C12N15/87 , C12N15/11
CPC分类号： C08F293/005 , A61K31/713 , A61K47/32 , A61K47/58 , C07K2319/10 , C08F293/00 , C12N15/111 , C12N15/113 , C12N15/87 , C12N2310/14 , C12N2310/315 , C12N2310/351 , C12N2320/32
摘要： Described herein are copolymers, and methods of making and utilizing such copolymers. Such copolymers have at least two blocks: a first block that has at least one unit that is hydrophilic at physiologic pH, and a second block that has hydrophobic groups. This second block further has at least one unit with a group that is anionic at about physiologic pH. The described copolymers are disruptive of a cellular membrane, including an extracellular membrane, an intracellular membrane, a vesicle, an organelle, an endosome, a liposome, or a red blood cell. Preferably, in certain instances, the copolymer disrupts the membrane and enters the intracellular environment. In specific examples, the copolymer is endosomolytic.
摘要翻译：本文描述的是共聚物，以及制备和利用这些共聚物的方法。这样的共聚物具有至少两个嵌段：在生理pH下具有至少一个亲水性单元的第一嵌段和具有疏水基团的第二嵌段。该第二嵌段还具有至少一个具有在约生理pH下为阴离子基团的单元。所述共聚物破坏细胞膜，包括细胞外膜，细胞内膜，囊泡，细胞器，内体，脂质体或红细胞。优选地，在某些情况下，共聚物破坏膜并进入细胞内环境。在具体实例中，共聚物是内体分解的。

10. 发明授权

US09339558B2 Micellic assemblies 有权
标题翻译：胶束装配
公开(公告)号：US09339558B2
公开(公告)日：2016-05-17
申请号：US12992525
申请日：2009-05-13
申请人： Patrick S. Stayton , Allan S. Hoffman , Anthony J. Convertine , Craig L. Duvall , Danielle Benoit , Robert W. Overell , Paul H. Johnson , Anna S. Gall , Mary G. Prieve , Amber E.E. Paschal , Charbel Diab , Priyadarsi De
发明人： Patrick S. Stayton , Allan S. Hoffman , Anthony J. Convertine , Craig L. Duvall , Danielle Benoit , Robert W. Overell , Paul H. Johnson , Anna S. Gall , Mary G. Prieve , Amber E.E. Paschal , Charbel Diab , Priyadarsi De
IPC分类号： A61K9/16 , A61K9/50 , C12N5/00 , C12N5/02 , C08F293/00 , C08F295/00 , C08F297/00 , A61K48/00 , A61K9/107 , C08F290/06 , C12N15/11 , C12N15/87
CPC分类号： A61K31/713 , A61K9/1075 , A61K47/549 , A61K47/58 , A61K47/60 , A61K47/6907 , A61K48/0041 , C08F290/062 , C08F293/005 , C12N15/111 , C12N15/87 , C12N2320/32 , Y02A50/463
摘要： Provided herein are micellic assemblies comprising a plurality of copolymers. In certain instances, micellic assemblies provided herein are pH sensitive particles.
摘要翻译：本文提供了包含多种共聚物的胶束组合物。在某些情况下，本文提供的胶束组件是pH敏感颗粒。

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