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1. 发明授权

US5206136A Rapid membrane affinity concentration assays 失效
标题翻译：快速膜亲和力浓度测定
公开(公告)号：US5206136A
公开(公告)日：1993-04-27
申请号：US925311
申请日：1992-07-31
申请人： Nobuo Monji , Carol-Ann Cole
发明人： Nobuo Monji , Carol-Ann Cole
IPC分类号： G01N33/538 , G01N33/543 , G01N33/545
CPC分类号： G01N33/545 , G01N33/538 , G01N33/54306 , Y10S435/803 , Y10S435/971 , Y10S435/974 , Y10S436/81
摘要： Rapid assays for analytes of interest in a fluid sample utilize a first conjugate of a labelled reactant that specifically binds to the analyte, and a second conjugate that binds to the analyte coupled to a polymer that has an affinity for a selected solid phase. The reaction components are incubated briefly, then contacted with the selected solid phase and the labelled components determined. Optional wash steps provide for enhanced sensitivity and specificity. When the analyte of interest is an antibody to HIV, the first reactant may be a synthetic, recombinant or native HIV antigen, and the second reactant may be protein A or an anti-immunoglobulin.
摘要翻译：对流体样品中感兴趣的分析物的快速测定利用特异性结合分析物的标记反应物的第一缀合物，以及结合与聚合物偶联的分析物的第二偶联物，所述分析物与选定的固相具有亲和性。将反应组分短暂孵育，然后与选定的固相接触并测定标记的组分。可选的洗涤步骤提供增强的灵敏度和特异性。当感兴趣的分析物是针对HIV的抗体时，第一反应物可以是合成的，重组的或天然的HIV抗原，第二反应物可以是蛋白A或抗免疫球蛋白。

2. 发明授权

US5439792A Cysteine thiol-protected peptides for use in immunoassays 失效
标题翻译：用于免疫测定的半胱氨酸硫醇保护的肽
公开(公告)号：US5439792A
公开(公告)日：1995-08-08
申请号：US140696
申请日：1993-10-21
申请人： James Blake , Carol-Ann Cole , Patrick F. Coleman , Nobuo Monji , John P. Montana
发明人： James Blake , Carol-Ann Cole , Patrick F. Coleman , Nobuo Monji , John P. Montana
IPC分类号： C07K1/107 , C07K14/16 , C07K17/02 , C12Q1/00
CPC分类号： C07K14/005 , C07K1/1077 , C07K17/02 , C12N2740/16022 , C12N2740/16122 , Y10S435/974
摘要： Peptides immunoreactive with antibodies to native proteins, and which have at least two cysteine residues that contribute to mimicking an epitope of the protein, are prepared with the cysteine thiol groups protected. When deprotected, the peptides have enhanced immunoreactivity. The peptides are particularly useful for detecting antibodies or antigens associated with retroviruses, including the clinically important lymphotropic retroviruses HIV-1, HIV-2, HTLV-I, and HTLV-II.
摘要翻译：与保护的半胱氨酸硫醇基团一起制备与天然蛋白质抗体免疫反应的肽，并且其具有有助于模拟蛋白质表位的至少两个半胱氨酸残基。当脱保护时，肽具有增强的免疫反应性。这些肽特别可用于检测与逆转录病毒相关的抗体或抗原，包括临床上重要的淋巴细胞逆转录病毒HIV-1，HIV-2，HTLV-1和HTLV-II。

3. 发明授权

US5206178A Membrane affinity concentration immunoassay 失效
标题翻译：膜亲和力浓度免疫测定
公开(公告)号：US5206178A
公开(公告)日：1993-04-27
申请号：US663193
申请日：1991-02-28
申请人： Nobuo Monji , Carol-Ann Cole
发明人： Nobuo Monji , Carol-Ann Cole
IPC分类号： G01N33/538 , G01N33/543 , G01N33/545
CPC分类号： G01N33/545 , G01N33/538 , G01N33/54306 , Y10S435/971 , Y10S436/81
摘要： Methods for determining the presence and/or concentration of an analyte in a biological fluid sample are disclosed. The methods generally include admixing in solution certain polymer/reactant and reporter/reactant conjugates along with the biological fluid sample suspected of containing the analyte, thereby forming ternary complexes. The separation of the complexes from the reaction mixture is achieved through the affinity of certain selected polymer compositions for various solid phases. Upon separation, the amount of reporter activity in the solution may be measured, and therefrom the presence and/or concentration of the analyte determined. Multiple analyses on a biological fluid sample suspected of containing one or more analytes may also be performed, using either a variety of different reporters or selected polymers having varied affinity for the solid phase.
摘要翻译：公开了用于确定生物流体样品中分析物的存在和/或浓度的方法。方法通常包括在溶液中混合某些聚合物/反应物和报告物/反应物共轭物以及怀疑含有分析物的生物流体样品，从而形成三元复合物。复合物与反应混合物的分离通过某些所选聚合物组合物对于各种固相的亲和力来实现。在分离时，可以测量溶液中的报告物活性的量，由此测定分析物的存在和/或浓度。怀疑含有一种或多种分析物的生物流体样品的多个分析也可以使用各种不同的报告物或对固相具有不同亲和力的所选聚合物进行。

4. 发明授权

US07144697B1 Synthetic antigen for the detection of antibodies immunoreactive with HIV virus 有权
标题翻译：用于检测与HIV病毒免疫反应的抗体的合成抗原
公开(公告)号：US07144697B1
公开(公告)日：2006-12-05
申请号：US09515014
申请日：2000-02-29
申请人： Patrick F. Coleman , Peter Chong-Dug Su , Nobuo Monji , Carol-Ann Cole
发明人： Patrick F. Coleman , Peter Chong-Dug Su , Nobuo Monji , Carol-Ann Cole
IPC分类号： C12Q1/70
CPC分类号： C07K14/005 , C12N2740/16222 , G01N33/56988 , G01N2469/20
摘要： Novel polypeptides are provided having substantially the same sequence as immunologically significant fragments of AIDS-related viruses. The polypeptides can be used as reagents in the determination of exposure of a human host to the virus. Of particular interest is the use of polypeptides in screening blood products.
摘要翻译：提供了与AIDS相关病毒的免疫学显着片段基本相同的序列的新型多肽。多肽可用作测定人宿主对病毒暴露的试剂。特别感兴趣的是使用多肽筛选血液制品。

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