专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明授权

US4323647A Steric hindrance enzyme immunoassay 失效
标题翻译：空肠阻抗酶免疫测定
公开(公告)号：US4323647A
公开(公告)日：1982-04-06
申请号：US197063
申请日：1980-10-15
申请人： Nobuo Monji , Albert Castro
发明人： Nobuo Monji , Albert Castro
IPC分类号： G01N33/538 , G01N33/542
CPC分类号： G01N33/538 , G01N33/542 , Y10S435/81 , Y10S435/964 , Y10S435/971 , Y10S435/975
摘要： A novel separation technique is described that is particularly useful for effecting separations in enzyme immunoassay procedures. A mixture, in an aqueous liquid vehicle, of (1), ligand-enzyme conjugate, and of (2), the conjugate bound through its ligand moiety to a receptor, is brought into contact with an insoluble, immobilized pseudo-substrate material, to which the enzyme normally binds. Free conjugate binds and becomes insoluble. Bound conjugate remains in the liquid phase. The ligand may be an antigen and the receptor, the antibody to the antigen.This separation technique makes feasible several sensitive immunoassay procedures. The material to be assayed may be, for example, rubella virus; hepatitis B surface antigen; gonorrhea antigen; the antibody to any of the foregoing; a general antibody, i.e., an immunoglobulin; a hormone such as choriomammotropin; a steroid, hapten, or the like.
摘要翻译：描述了一种新颖的分离技术，其特别可用于实现酶免疫测定方法中的分离。在水性液体载体中，（1）配体 - 酶偶联物和（2）的混合物，通过其配体部分与受体结合的缀合物与不溶性固定的假底物材料接触，酶通常结合到其上。游离共轭物结合并变得不溶。结合的结合物保留在液相中。配体可以是抗原和受体，即抗原的抗体。这种分离技术使几种敏感的免疫测定方法成为可行。要测定的材料可以是例如风疹病毒; 乙型肝炎表面抗原; 淋病抗原针对上述任何一种的抗体; 一般抗体，即免疫球蛋白; 一种激素如脉络膜激素; 类固醇，半抗原等。

IPRDB

热门服务

关于我们

友情链接

联系方式