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1. 发明申请

US20130318653A1 AROGENATE DEHYDRATASES AND LIGNIFICATION 审中-公开
标题翻译： AROGENATE DEHYDRATASES和LIGNIFICATION
公开(公告)号：US20130318653A1
公开(公告)日：2013-11-28
申请号：US13884472
申请日：2011-11-09
申请人： Norman G. Lewis , Laurence B. Davin , Oliver R.A. Corea , Sung-Jin Kim
发明人： Norman G. Lewis , Laurence B. Davin , Oliver R.A. Corea , Sung-Jin Kim
IPC分类号： C12N15/82
CPC分类号： C12N15/8255 , C12N9/88
摘要： Provided are methods for decreasing carbon flow into lignin in plants, comprising reducing or eliminating, using mutagenesis and/or recombinant means, expression and/or activity of at least one chloroplast-localized arogenate dehydratase (ADT) sufficient to reduce phenylalanine (Phe) availability for metabolism into Phe-derived phenylpropanoids, wherein the amount, level or distribution of lignin is reduced relative to control plants. In particular aspects, the plant has a plurality of chloroplast-localized ADTs, and reducing or eliminating comprises reducing or eliminating expression and/or activity of at least two of the plurality of ADTs. Also provided are recombinant plants or parts or cells thereof, comprising at least one mutation, genetic alteration or transgene that reduces or eliminates the expression and/or activity of at least one chloroplast-localized ADT, wherein the amount, level or distribution of lignin is reduced relative to normal. Further provided are reduced lignin plant products.
摘要翻译：提供了减少植物中进入木质素的碳流的方法，包括使用诱变和/或重组手段减少或消除足以降低苯丙氨酸（Phe）可利用性的至少一种叶绿体定位的雄激素脱水酶（ADT）的表达和/或活性用于代谢为Phe衍生的苯丙素类，其中相对于对照植物，木质素的量，水平或分布降低。在具体方面，植物具有多个叶绿体定位的ADT，并且减少或消除包括减少或消除多个ADT中的至少两个的表达和/或活性。还提供了重组植物或其部分或细胞，其包含至少一种突变，遗传改变或转基因，其减少或消除至少一种叶绿体定位的ADT的表达和/或活性，其中木质素的量，水平或分布为相对于正常减少。还提供了减少的木质素植物产品。

2. 发明申请

US20100031398A1 GENES ENCODING CHAVICOL/EUGENOL SYNTHASE FROM THE CREOSOTE BUSH LARREA TRIDENTATA 有权
标题翻译：基因编码CHAVICOL / EUGENOL SYNTHASE从CREOSOTE BUSH LARREA TRIDENTATA
公开(公告)号：US20100031398A1
公开(公告)日：2010-02-04
申请号：US12307343
申请日：2007-05-29
申请人： Norman G. Lewis , Laurence B. Davin , Sung-Jin Kim , Daniel Giddings Vassão , Ann M. Patten , Dietmar Eichinger
发明人： Norman G. Lewis , Laurence B. Davin , Sung-Jin Kim , Daniel Giddings Vassão , Ann M. Patten , Dietmar Eichinger
IPC分类号： A01H5/00 , C07H21/00 , C07K14/415 , C12N15/63 , C12N5/04 , C12N1/19 , C12N1/21 , C12N5/07 , C07C57/00 , C07K16/00 , C12P7/22 , C12P7/06 , A01H5/10
CPC分类号： A01H5/02 , C12N9/0004 , C12N15/8243 , C12P7/22 , Y02E50/17
摘要： Particular aspects provide novel methods for redirecting carbon allocation in plants or cell culture from lignification to inherently more useful and tractable materials, and to facilitate the generation of, e.g., biofuels from the remaining plant roculture biomass. Particular aspects provided novel methods for converting monolignols into allyl/propenyl phenols, and for chavicol/eugenol formation or production. Additional aspects relate to the discovery of novel chavicol/eugenol synthases that convert p-coumaryl/coniferyl alcohol esters into chavicol/eugenol, and to novel compositions (e.g., novel proteins and nucleic acids encoding same), and novel methods using same for producing or forming chavicol/eugenol and other derivatives in cell culture and/or genetically modified plants, and for re-engineering the composition of plant biomass. Particular aspects provide novel methods for generation in culture or in planta of liquid/combustible allyl/propenyl phenols, and these phenolic products are utilized for (non-ethanol) biofuel/bioenergy purposes, while the remaining plant biomass facilitates the generation of other biofuels.
摘要翻译：特定方面提供了用于将植物或细胞培养物中的碳分配从木质化重定向到本来更有用和易处理的材料的新颖方法，并且促进从剩余的植物培养生物质中产生例如生物燃料。具体方面提供了将单螺烯醇转化为烯丙基/丙烯基酚，以及用于切维霉素/丁子香酚生成或生产的新方法。另外的方面涉及将对香豆醇/松柏烯醇酯转化成切维霉素/丁子香酚的新型酪维沙星/丁子香酚合成酶的发现，以及新型组合物（例如，编码其的新型蛋白质和核酸）的新发现，以及用于制备或在细胞培养和/或遗传修饰植物中形成辣椒素/丁子香酚和其他衍生物，并重新设计植物生物量的组成。具体方面提供了用于在液体/可燃烯丙基/丙烯基苯酚的培养或植物中产生的新方法，并且这些酚类产物用于（非乙醇）生物燃料/生物能源目的，而剩余的植物生物质便于生成其它生物燃料。

3. 发明授权

US09131648B2 Genes encoding chavicol/eugenol synthase from the creosote bush Larrea tridentata 有权
标题翻译：编码来自杂酚油灌木Larrea tridentata的chavicol / Eugenol合酶的基因
公开(公告)号：US09131648B2
公开(公告)日：2015-09-15
申请号：US12307343
申请日：2007-05-29
申请人： Norman G. Lewis , Laurence B. Davin , Sung-Jin Kim , Daniel Giddings Vassão , Ann M. Patten , Dietmar Eichinger
发明人： Norman G. Lewis , Laurence B. Davin , Sung-Jin Kim , Daniel Giddings Vassão , Ann M. Patten , Dietmar Eichinger
IPC分类号： C12N15/00 , C12N15/82 , C12N5/04 , C12N5/10 , C12N15/87 , A01H5/02 , C12N9/02 , C12P7/22
CPC分类号： A01H5/02 , C12N9/0004 , C12N15/8243 , C12P7/22 , Y02E50/17
摘要： Particular aspects provide novel methods for redirecting carbon allocation in plants or cell culture from lignification to inherently more useful and tractable materials, and to facilitate the generation of, e.g., biofuels from the remaining plant ro culture biomass. Particular aspects provided novel methods for converting monolignols into allyl/propenyl phenols, and for chavicol/eugenol formation or production. Additional aspects relate to the discovery of novel chavicol/eugenol synthases that convert p-coumaryl/coniferyl alcohol esters into chavicol/eugenol, and to novel compositions (e.g., novel proteins and nucleic acids encoding same), and novel methods using same for producing or forming chavicol/eugenol and other derivatives in cell culture and/or genetically modified plants, and for re-engineering the composition of plant biomass. Particular aspects provide novel methods for generation in culture or in planta of liquid/combustible allyl/propenyl phenols, and these phenolic products are utilized for (non-ethanol) biofuel/bioenergy purposes, while the remaining plant biomass facilitates the generation of other biofuels.
摘要翻译：特定方面提供了用于将植物或细胞培养物中的碳分配从木质化重定向到本来更有用和易于处理的材料的新颖方法，并且促进从剩余的植物培养生物质中产生例如生物燃料。具体方面提供了将单螺烯醇转化为烯丙基/丙烯基酚，以及用于切维霉素/丁子香酚生成或生产的新方法。另外的方面涉及将对香豆醇/松柏烯醇酯转化成切维霉素/丁子香酚的新型酪维沙星/丁子香酚合成酶的发现，以及新型组合物（例如，编码其的新型蛋白质和核酸）的新发现，以及用于制备或在细胞培养和/或遗传修饰植物中形成辣椒素/丁子香酚和其他衍生物，并重新设计植物生物量的组成。具体方面提供了用于在液体/可燃烯丙基/丙烯基苯酚的培养或植物中产生的新方法，并且这些酚类产物用于（非乙醇）生物燃料/生物能源目的，而剩余的植物生物质便于生成其它生物燃料。

4. 发明授权

US06703229B2 Aryl propenal double bond reductase 有权
标题翻译：芳基丙烯双键还原酶
公开(公告)号：US06703229B2
公开(公告)日：2004-03-09
申请号：US09820096
申请日：2001-03-27
申请人： Hiroyuki Kasahara , Laurence B. Davin , Norman G. Lewis
发明人： Hiroyuki Kasahara , Laurence B. Davin , Norman G. Lewis
IPC分类号： C12N902
CPC分类号： C12N9/001
摘要： In one aspect, the present invention provides isolated nucleic acid molecules that each: (a) encode an aryl propenal double bond reductase; and (b) hybridize to a nucleic acid molecule consisting of the complement of the nucleic acid sequence set forth in SEQ ID NO:1 under defined conditions. The present invention also provides isolated aryl propenal double bond reductases. In other aspects, the present invention provides methods of enhancing or inhibiting the expression of aryl propenal double bond reductases in a plant.
摘要翻译：一方面，本发明提供分离的核酸分子，其各自：（a）编码芳基丙烯醛双键还原酶; 和（b）在确定的条件下与由SEQ ID NO：1所示的核酸序列的互补体组成的核酸分子杂交。本发明还提供了分离的芳基丙烯醛双键还原酶。在其它方面，本发明提供增强或抑制植物中芳基丙烯醛双键还原酶表达的方法。

5. 发明授权

US06210942B1 Recombinant pinoresinol/lariciresinol reductase, recombinant dirigent protein, and methods of use 有权
标题翻译：重组苯并辛醇/椴树醇还原酶，重组蛋白质和重组蛋白质，以及使用方法
公开(公告)号：US06210942B1
公开(公告)日：2001-04-03
申请号：US09475316
申请日：1999-12-30
申请人： Norman G. Lewis , Laurence B. Davin , Albena T. Dinkova-Kostova , Masayuki Fujita , David R. Gang , Simo Sarkanen , Joshua D. Ford
发明人： Norman G. Lewis , Laurence B. Davin , Albena T. Dinkova-Kostova , Masayuki Fujita , David R. Gang , Simo Sarkanen , Joshua D. Ford
IPC分类号： C12N900
CPC分类号： C12N9/0004 , C07K14/415 , C12N15/8243 , C12P7/22 , C12P17/04
摘要： Dirigent proteins and pinoresinol/lariciresinol reductases have been isolated, together with cDNAs encoding dirigent proteins and pinoresinol/lariciresinol reductases. Accordingly, isolated DNA sequences are provided which code for the expression of dirigent proteins and pinoresinol/lariciresinol reductases. In other aspects, replicable recombinant cloning vehicles are provided which code for dirigent proteins or pinoresinol/lariciresinol reductases or for a base sequence sufficiently complementary to at least a portion of dirigent protein or pinoresinol/lariciresinol reductase DNA or RNA to enable hybridization therewith. In yet other aspects, modified host cells are provided that have been transformed, transfected, infected and/or injected with a recombinant cloning vehicle and/or DNA sequence encoding dirigent protein or pinoresinol/lariciresinol reductase. Thus, systems and methods are provided for the recombinant expression of dirigent proteins and/or pinoresinol/lariciresinol reductases.
摘要翻译：已经分离了多种蛋白质和pinoresinol / lariciresinol还原酶，以及编码dibigent蛋白质和pinoresinol / lariciresinol还原酶的cDNA。因此，提供了分离的DNA序列，其编码dirigent蛋白质和pinoresinol / lariciresinol还原酶的表达。在其他方面，提供可复制的重组克隆载体，其编码dirigent蛋白质或pinoresinol / lariciresinol还原酶或与至少一部分dirigent蛋白质或pinoresinol / lariciresinol还原酶DNA或RNA充分互补的碱基序列，以使其能够进行杂交。在另外的方面，提供已经用编码dirigent蛋白质或pinoresinol / lariciresinol还原酶的重组克隆载体和/或DNA序列转化，转染，感染和/或注射的修饰的宿主细胞。因此，提供了用于重组表达dirigent蛋白质和/或pinoresinol / lariciresinol还原酶的系统和方法。

6. 发明授权

US06911330B1 Recombinant secoisolariciciresinol dehydrogensase, and methods of use 有权
标题翻译：重组分裂异丝氨酸脱氢酶，以及使用方法
公开(公告)号：US06911330B1
公开(公告)日：2005-06-28
申请号：US09673918
申请日：1999-04-23
申请人： Zhi-Qiang Xia , Michael A. Costa , Laurence B. Davin , Norman G. Lewis
发明人： Zhi-Qiang Xia , Michael A. Costa , Laurence B. Davin , Norman G. Lewis
IPC分类号： C12N15/09 , C07H21/04 , C12N1/15 , C12N1/19 , C12N1/20 , C12N1/21 , C12N5/00 , C12N5/10 , C12N9/04 , C12N15/00 , C12P21/02 , C12Q1/68
CPC分类号： C12N9/0006
摘要： A secoisolanciresinol dehydrogenase protein has been isolated from Forsythia intermedia, together with cDNAs encoding secoisolariciresinol dehydrogenase from this species. Accordingly, isolated DNA sequences are provided which code for the expression of secoisolariciresinol dehydrogenase. In other aspects, the present invention is directed to replicable recombinant cloning vehicles comprising a nucleic acid sequence which codes for a secoisolariciresinol dehydrogenase protein, or to a base sequence sufficiently complementary to at least a portion of a secoisolariciresinol dehydrogenase DNA or RNA to enable hybridization therewith. Thus, systems and methods are provided for the recombinant expression of secoisolariciresinol dehydrogenases that may be used to facilitate the production, isolation and purification of significant quantities of recombinant secoisolariciresinol dehydrogenase for subsequent use, to obtain expression or enhanced expression of secoisolariciresinol dehydrogenase in plants in order to enhance, or otherwise alter, lignan biosynthesis, or may be otherwise employed for the regulation or expression of secoisolariciresinol dehydrogenase.
摘要翻译：从间叶连翘中分离出一种异恶唑烷醇脱氢酶蛋白，以及来自该物种的编码分离异硫氰酸脱氢酶的cDNA。因此，提供了分离的DNA序列，其编码表达了二异硫氰酸脱氢酶。在其他方面，本发明涉及可复制的重组克隆载体，其包含编码一种间歇异硫氰菊酯脱氢酶蛋白的核酸序列，或与至少一部分间位异辛烯脱氢酶DNA或RNA充分互补的碱基序列，以使其能够与其进行杂交。因此，提供系统和方法用于重组表达的二硫代亚铁氰菊醇脱氢酶，其可用于促进大量的重组分离异硫辛烯醇脱氢酶的生产，分离和纯化用于随后的使用，以顺序获得在植物中的顺式表达或增强的表达，以增强或以其它方式改变木脂素生物合成，或者可以另外用于调节或表达分离异癸烯醇脱氢酶。

7. 发明授权

US06635459B1 Nucleotide sequences encoding pinoresinol/lariciresinol reductase proteins and their methods of use 有权
标题翻译：编码尖晶石醇/拉辛啶醇还原酶蛋白的核苷酸序列及其使用方法
公开(公告)号：US06635459B1
公开(公告)日：2003-10-21
申请号：US09704640
申请日：2000-11-02
申请人： Norman G. Lewis , Laurence B. Davin , Albena T. Dinkova-Kostova , Masayuki Fujita , David R. Gang , Simo Sarkanen , Joshua D. Ford
发明人： Norman G. Lewis , Laurence B. Davin , Albena T. Dinkova-Kostova , Masayuki Fujita , David R. Gang , Simo Sarkanen , Joshua D. Ford
IPC分类号： C12N904
CPC分类号： C12N9/0004 , C07K14/415 , C12N15/8243 , C12P7/22 , C12P17/04
摘要： Dirigent proteins and pinoresinol/lariciresinol reductases have been isolated, together with cDNAs encoding dirigent proteins and pinoresinol/lariciresinol reductases. Accordingly, isolated DNA sequences are provided from source species Forsythia intermedia, Thuja plicata, Tsuga heterophylla, Eucommia ulmoides, Linum usitatissimum, and Schisandra chinensis, which code for the expression of dirigent proteins and pinoresinol/lariciresinol reductases. In other aspects, replicable recombinant cloning vehicles are provided which code for dirigent proteins or pinoresinol/lariciresinol reductases or for a base sequence sufficiently complementary to at least a portion of dirigent protein or pinoresinol/lariciresinol reductase DNA or RNA to enable hybridization therewith. In yet other aspects, modified host cells are provided that have been transformed, transfected, infected and/or injected with a recombinant cloning vehicle and/or DNA sequence encoding dirigent protein or pinoresinol/lariciresinol reductase. Thus, systems and methods are provided for the recombinant expression of dirigent proteins and/or pinoresinol/lariciresinol reductases.
摘要翻译：已经分离了多种蛋白质和pinoresinol / lariciresinol还原酶，以及编码dibigent蛋白质和pinoresinol / lariciresinol还原酶的cDNA。因此，分离的DNA序列从源物种连翘，中华of藜，紫穗槐，杜仲，杜鹃花，中国五味子等提供，代表了dirigent蛋白质和pinoresinol / lariciresinol还原酶的表达。在其他方面，提供可复制的重组克隆载体，其编码dirigent蛋白质或pinoresinol / lariciresinol还原酶或与至少一部分dirigent蛋白质或pinoresinol / lariciresinol还原酶DNA或RNA充分互补的碱基序列，以使其能够进行杂交。在另外的方面，提供已经用编码dirigent蛋白质或pinoresinol / lariciresinol还原酶的重组克隆载体和/或DNA序列转化，转染，感染和/或注射的修饰的宿主细胞。因此，提供了用于重组表达dirigent蛋白质和/或pinoresinol / lariciresinol还原酶的系统和方法。

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