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1. 发明授权

US06635459B1 Nucleotide sequences encoding pinoresinol/lariciresinol reductase proteins and their methods of use 有权
标题翻译：编码尖晶石醇/拉辛啶醇还原酶蛋白的核苷酸序列及其使用方法
公开(公告)号：US06635459B1
公开(公告)日：2003-10-21
申请号：US09704640
申请日：2000-11-02
申请人： Norman G. Lewis , Laurence B. Davin , Albena T. Dinkova-Kostova , Masayuki Fujita , David R. Gang , Simo Sarkanen , Joshua D. Ford
发明人： Norman G. Lewis , Laurence B. Davin , Albena T. Dinkova-Kostova , Masayuki Fujita , David R. Gang , Simo Sarkanen , Joshua D. Ford
IPC分类号： C12N904
CPC分类号： C12N9/0004 , C07K14/415 , C12N15/8243 , C12P7/22 , C12P17/04
摘要： Dirigent proteins and pinoresinol/lariciresinol reductases have been isolated, together with cDNAs encoding dirigent proteins and pinoresinol/lariciresinol reductases. Accordingly, isolated DNA sequences are provided from source species Forsythia intermedia, Thuja plicata, Tsuga heterophylla, Eucommia ulmoides, Linum usitatissimum, and Schisandra chinensis, which code for the expression of dirigent proteins and pinoresinol/lariciresinol reductases. In other aspects, replicable recombinant cloning vehicles are provided which code for dirigent proteins or pinoresinol/lariciresinol reductases or for a base sequence sufficiently complementary to at least a portion of dirigent protein or pinoresinol/lariciresinol reductase DNA or RNA to enable hybridization therewith. In yet other aspects, modified host cells are provided that have been transformed, transfected, infected and/or injected with a recombinant cloning vehicle and/or DNA sequence encoding dirigent protein or pinoresinol/lariciresinol reductase. Thus, systems and methods are provided for the recombinant expression of dirigent proteins and/or pinoresinol/lariciresinol reductases.
摘要翻译：已经分离了多种蛋白质和pinoresinol / lariciresinol还原酶，以及编码dibigent蛋白质和pinoresinol / lariciresinol还原酶的cDNA。因此，分离的DNA序列从源物种连翘，中华of藜，紫穗槐，杜仲，杜鹃花，中国五味子等提供，代表了dirigent蛋白质和pinoresinol / lariciresinol还原酶的表达。在其他方面，提供可复制的重组克隆载体，其编码dirigent蛋白质或pinoresinol / lariciresinol还原酶或与至少一部分dirigent蛋白质或pinoresinol / lariciresinol还原酶DNA或RNA充分互补的碱基序列，以使其能够进行杂交。在另外的方面，提供已经用编码dirigent蛋白质或pinoresinol / lariciresinol还原酶的重组克隆载体和/或DNA序列转化，转染，感染和/或注射的修饰的宿主细胞。因此，提供了用于重组表达dirigent蛋白质和/或pinoresinol / lariciresinol还原酶的系统和方法。

2. 发明授权

US06210942B1 Recombinant pinoresinol/lariciresinol reductase, recombinant dirigent protein, and methods of use 有权
标题翻译：重组苯并辛醇/椴树醇还原酶，重组蛋白质和重组蛋白质，以及使用方法
公开(公告)号：US06210942B1
公开(公告)日：2001-04-03
申请号：US09475316
申请日：1999-12-30
申请人： Norman G. Lewis , Laurence B. Davin , Albena T. Dinkova-Kostova , Masayuki Fujita , David R. Gang , Simo Sarkanen , Joshua D. Ford
发明人： Norman G. Lewis , Laurence B. Davin , Albena T. Dinkova-Kostova , Masayuki Fujita , David R. Gang , Simo Sarkanen , Joshua D. Ford
IPC分类号： C12N900
CPC分类号： C12N9/0004 , C07K14/415 , C12N15/8243 , C12P7/22 , C12P17/04
摘要： Dirigent proteins and pinoresinol/lariciresinol reductases have been isolated, together with cDNAs encoding dirigent proteins and pinoresinol/lariciresinol reductases. Accordingly, isolated DNA sequences are provided which code for the expression of dirigent proteins and pinoresinol/lariciresinol reductases. In other aspects, replicable recombinant cloning vehicles are provided which code for dirigent proteins or pinoresinol/lariciresinol reductases or for a base sequence sufficiently complementary to at least a portion of dirigent protein or pinoresinol/lariciresinol reductase DNA or RNA to enable hybridization therewith. In yet other aspects, modified host cells are provided that have been transformed, transfected, infected and/or injected with a recombinant cloning vehicle and/or DNA sequence encoding dirigent protein or pinoresinol/lariciresinol reductase. Thus, systems and methods are provided for the recombinant expression of dirigent proteins and/or pinoresinol/lariciresinol reductases.
摘要翻译：已经分离了多种蛋白质和pinoresinol / lariciresinol还原酶，以及编码dibigent蛋白质和pinoresinol / lariciresinol还原酶的cDNA。因此，提供了分离的DNA序列，其编码dirigent蛋白质和pinoresinol / lariciresinol还原酶的表达。在其他方面，提供可复制的重组克隆载体，其编码dirigent蛋白质或pinoresinol / lariciresinol还原酶或与至少一部分dirigent蛋白质或pinoresinol / lariciresinol还原酶DNA或RNA充分互补的碱基序列，以使其能够进行杂交。在另外的方面，提供已经用编码dirigent蛋白质或pinoresinol / lariciresinol还原酶的重组克隆载体和/或DNA序列转化，转染，感染和/或注射的修饰的宿主细胞。因此，提供了用于重组表达dirigent蛋白质和/或pinoresinol / lariciresinol还原酶的系统和方法。

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