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1. 发明申请

US20130318653A1 AROGENATE DEHYDRATASES AND LIGNIFICATION 审中-公开
标题翻译： AROGENATE DEHYDRATASES和LIGNIFICATION
公开(公告)号：US20130318653A1
公开(公告)日：2013-11-28
申请号：US13884472
申请日：2011-11-09
申请人： Norman G. Lewis , Laurence B. Davin , Oliver R.A. Corea , Sung-Jin Kim
发明人： Norman G. Lewis , Laurence B. Davin , Oliver R.A. Corea , Sung-Jin Kim
IPC分类号： C12N15/82
CPC分类号： C12N15/8255 , C12N9/88
摘要： Provided are methods for decreasing carbon flow into lignin in plants, comprising reducing or eliminating, using mutagenesis and/or recombinant means, expression and/or activity of at least one chloroplast-localized arogenate dehydratase (ADT) sufficient to reduce phenylalanine (Phe) availability for metabolism into Phe-derived phenylpropanoids, wherein the amount, level or distribution of lignin is reduced relative to control plants. In particular aspects, the plant has a plurality of chloroplast-localized ADTs, and reducing or eliminating comprises reducing or eliminating expression and/or activity of at least two of the plurality of ADTs. Also provided are recombinant plants or parts or cells thereof, comprising at least one mutation, genetic alteration or transgene that reduces or eliminates the expression and/or activity of at least one chloroplast-localized ADT, wherein the amount, level or distribution of lignin is reduced relative to normal. Further provided are reduced lignin plant products.
摘要翻译：提供了减少植物中进入木质素的碳流的方法，包括使用诱变和/或重组手段减少或消除足以降低苯丙氨酸（Phe）可利用性的至少一种叶绿体定位的雄激素脱水酶（ADT）的表达和/或活性用于代谢为Phe衍生的苯丙素类，其中相对于对照植物，木质素的量，水平或分布降低。在具体方面，植物具有多个叶绿体定位的ADT，并且减少或消除包括减少或消除多个ADT中的至少两个的表达和/或活性。还提供了重组植物或其部分或细胞，其包含至少一种突变，遗传改变或转基因，其减少或消除至少一种叶绿体定位的ADT的表达和/或活性，其中木质素的量，水平或分布为相对于正常减少。还提供了减少的木质素植物产品。

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