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1. 发明授权

US09131648B2 Genes encoding chavicol/eugenol synthase from the creosote bush Larrea tridentata 有权
标题翻译：编码来自杂酚油灌木Larrea tridentata的chavicol / Eugenol合酶的基因
公开(公告)号：US09131648B2
公开(公告)日：2015-09-15
申请号：US12307343
申请日：2007-05-29
申请人： Norman G. Lewis , Laurence B. Davin , Sung-Jin Kim , Daniel Giddings Vassão , Ann M. Patten , Dietmar Eichinger
发明人： Norman G. Lewis , Laurence B. Davin , Sung-Jin Kim , Daniel Giddings Vassão , Ann M. Patten , Dietmar Eichinger
IPC分类号： C12N15/00 , C12N15/82 , C12N5/04 , C12N5/10 , C12N15/87 , A01H5/02 , C12N9/02 , C12P7/22
CPC分类号： A01H5/02 , C12N9/0004 , C12N15/8243 , C12P7/22 , Y02E50/17
摘要： Particular aspects provide novel methods for redirecting carbon allocation in plants or cell culture from lignification to inherently more useful and tractable materials, and to facilitate the generation of, e.g., biofuels from the remaining plant ro culture biomass. Particular aspects provided novel methods for converting monolignols into allyl/propenyl phenols, and for chavicol/eugenol formation or production. Additional aspects relate to the discovery of novel chavicol/eugenol synthases that convert p-coumaryl/coniferyl alcohol esters into chavicol/eugenol, and to novel compositions (e.g., novel proteins and nucleic acids encoding same), and novel methods using same for producing or forming chavicol/eugenol and other derivatives in cell culture and/or genetically modified plants, and for re-engineering the composition of plant biomass. Particular aspects provide novel methods for generation in culture or in planta of liquid/combustible allyl/propenyl phenols, and these phenolic products are utilized for (non-ethanol) biofuel/bioenergy purposes, while the remaining plant biomass facilitates the generation of other biofuels.
摘要翻译：特定方面提供了用于将植物或细胞培养物中的碳分配从木质化重定向到本来更有用和易于处理的材料的新颖方法，并且促进从剩余的植物培养生物质中产生例如生物燃料。具体方面提供了将单螺烯醇转化为烯丙基/丙烯基酚，以及用于切维霉素/丁子香酚生成或生产的新方法。另外的方面涉及将对香豆醇/松柏烯醇酯转化成切维霉素/丁子香酚的新型酪维沙星/丁子香酚合成酶的发现，以及新型组合物（例如，编码其的新型蛋白质和核酸）的新发现，以及用于制备或在细胞培养和/或遗传修饰植物中形成辣椒素/丁子香酚和其他衍生物，并重新设计植物生物量的组成。具体方面提供了用于在液体/可燃烯丙基/丙烯基苯酚的培养或植物中产生的新方法，并且这些酚类产物用于（非乙醇）生物燃料/生物能源目的，而剩余的植物生物质便于生成其它生物燃料。

2. 发明申请

US20100031398A1 GENES ENCODING CHAVICOL/EUGENOL SYNTHASE FROM THE CREOSOTE BUSH LARREA TRIDENTATA 有权
标题翻译：基因编码CHAVICOL / EUGENOL SYNTHASE从CREOSOTE BUSH LARREA TRIDENTATA
公开(公告)号：US20100031398A1
公开(公告)日：2010-02-04
申请号：US12307343
申请日：2007-05-29
申请人： Norman G. Lewis , Laurence B. Davin , Sung-Jin Kim , Daniel Giddings Vassão , Ann M. Patten , Dietmar Eichinger
发明人： Norman G. Lewis , Laurence B. Davin , Sung-Jin Kim , Daniel Giddings Vassão , Ann M. Patten , Dietmar Eichinger
IPC分类号： A01H5/00 , C07H21/00 , C07K14/415 , C12N15/63 , C12N5/04 , C12N1/19 , C12N1/21 , C12N5/07 , C07C57/00 , C07K16/00 , C12P7/22 , C12P7/06 , A01H5/10
CPC分类号： A01H5/02 , C12N9/0004 , C12N15/8243 , C12P7/22 , Y02E50/17
摘要： Particular aspects provide novel methods for redirecting carbon allocation in plants or cell culture from lignification to inherently more useful and tractable materials, and to facilitate the generation of, e.g., biofuels from the remaining plant roculture biomass. Particular aspects provided novel methods for converting monolignols into allyl/propenyl phenols, and for chavicol/eugenol formation or production. Additional aspects relate to the discovery of novel chavicol/eugenol synthases that convert p-coumaryl/coniferyl alcohol esters into chavicol/eugenol, and to novel compositions (e.g., novel proteins and nucleic acids encoding same), and novel methods using same for producing or forming chavicol/eugenol and other derivatives in cell culture and/or genetically modified plants, and for re-engineering the composition of plant biomass. Particular aspects provide novel methods for generation in culture or in planta of liquid/combustible allyl/propenyl phenols, and these phenolic products are utilized for (non-ethanol) biofuel/bioenergy purposes, while the remaining plant biomass facilitates the generation of other biofuels.
摘要翻译：特定方面提供了用于将植物或细胞培养物中的碳分配从木质化重定向到本来更有用和易处理的材料的新颖方法，并且促进从剩余的植物培养生物质中产生例如生物燃料。具体方面提供了将单螺烯醇转化为烯丙基/丙烯基酚，以及用于切维霉素/丁子香酚生成或生产的新方法。另外的方面涉及将对香豆醇/松柏烯醇酯转化成切维霉素/丁子香酚的新型酪维沙星/丁子香酚合成酶的发现，以及新型组合物（例如，编码其的新型蛋白质和核酸）的新发现，以及用于制备或在细胞培养和/或遗传修饰植物中形成辣椒素/丁子香酚和其他衍生物，并重新设计植物生物量的组成。具体方面提供了用于在液体/可燃烯丙基/丙烯基苯酚的培养或植物中产生的新方法，并且这些酚类产物用于（非乙醇）生物燃料/生物能源目的，而剩余的植物生物质便于生成其它生物燃料。

3. 发明申请

US20120135928A1 METHOD FOR PURIFYING RECOMBINANT FSH 有权
标题翻译：净化重组FSH的方法
公开(公告)号：US20120135928A1
公开(公告)日：2012-05-31
申请号：US13262622
申请日：2010-04-01
申请人： Christian Scheckermann , Dietmar Eichinger , Stefan Arnold
发明人： Christian Scheckermann , Dietmar Eichinger , Stefan Arnold
IPC分类号： A61K38/24 , A61P15/08 , C07K14/59 , C07K1/36 , B01D15/38
CPC分类号： C07K14/59 , A61K38/00 , C07K1/36 , C12P21/005
摘要： The present invention relates to a method for purifying a recombinant follicle stimulating hormone (FSH) or re-combinant FSH variant. The method comprises the steps of subjecting a liquid containing a recombinant FSH or recombinant FSH variant to an anion exchange chromatography, to a hydrophobic interaction chromatography, and to a dye affinity chromatography, wherein these chromatographies may be performed in any order, and wherein the method neither comprises a weak anion exchange chromatography nor a reverse phase chromatography. The method of purification results in a high yield of recombinant FSH having a desired degree of purity. The obtained FSH is especially useful for the prophylaxis and treatment of disorders and medical indications where FSH preparations are considered as useful remedies.
摘要翻译：本发明涉及一种纯化重组卵泡刺激素（FSH）或重组FSH变体的方法。该方法包括以下步骤：将含有重组FSH或重组FSH变体的液体进行阴离子交换层析，到疏水相互作用色谱法和染料亲和层析，其中这些色谱可以以任何顺序进行，并且其中所述方法既不包括弱阴离子交换色谱法也不包括反相色谱法。纯化的方法导致具有所需纯度的重组FSH的高产率。获得的FSH对于预防和治疗疾病和医疗适应症尤其有用，其中FSH制剂被认为是有用的补救措施。

4. 发明授权

US09096683B2 Method for purifying recombinant FSH 有权
标题翻译：重组FSH的纯化方法
公开(公告)号：US09096683B2
公开(公告)日：2015-08-04
申请号：US13262622
申请日：2010-04-01
申请人： Christian Scheckermann , Dietmar Eichinger , Stefan Arnold
发明人： Christian Scheckermann , Dietmar Eichinger , Stefan Arnold
IPC分类号： A61K38/24 , A61P15/08 , C07K1/36 , C07K14/59
CPC分类号： C07K14/59 , A61K38/00 , C07K1/36 , C12P21/005
摘要： The present invention relates to a method for purifying a recombinant follicle stimulating hormone (FSH) or recombinant FSH variant. The method comprises the steps of subjecting a liquid containing a recombinant FSH or recombinant FSH variant to an anion exchange chromatography, to a hydrophobic interaction chromatography, and to a dye affinity chromatography, wherein these chromatographies may be performed in any order, and wherein the method neither comprises a weak anion exchange chromatography nor a reverse phase chromatography. The method of purification results in a high yield of recombinant FSH having a desired degree of purity. The obtained FSH is especially useful for the prophylaxis and treatment of disorders and medical indications where FSH preparations are considered as useful remedies.
摘要翻译：本发明涉及纯化重组卵泡刺激素（FSH）或重组FSH变体的方法。该方法包括以下步骤：将含有重组FSH或重组FSH变体的液体进行阴离子交换层析，到疏水相互作用层析和染料亲和层析，其中这些色谱可以以任何顺序进行，并且其中所述方法既不包括弱阴离子交换色谱法也不包括反相色谱法。纯化的方法导致具有所需纯度的重组FSH的高产率。获得的FSH对于预防和治疗疾病和医疗适应症尤其有用，其中FSH制剂被认为是有用的补救措施。

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