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1. 发明申请

US20090191098A1 High Density Plate Filler 审中-公开
标题翻译：高密度平板填料
公开(公告)号：US20090191098A1
公开(公告)日：2009-07-30
申请号：US12419015
申请日：2009-04-06
申请人： Nigel P. Beard , Mark T. Reed
发明人： Nigel P. Beard , Mark T. Reed
IPC分类号： B01L3/00
CPC分类号： G01N35/028 , B01L3/0268 , B01L3/0293 , B01L3/5025 , B01L3/50851 , B01L3/50853 , B01L3/563 , B01L2200/021 , B01L2200/025 , B01L2200/0605 , B01L2200/0621 , B01L2200/0642 , B01L2200/0684 , B01L2300/046 , B01L2300/0829 , B01L2300/0864 , B01L2300/1805 , B01L2400/0406 , B01L2400/0409 , B01L2400/0487 , G01N35/1074 , G01N2035/1034
摘要： A filling apparatus for filling a microplate. The microplate having a plurality of wells each sized to receive an assay. The filling apparatus can comprise an assay input layer having a first surface and an opposing second surface. The assay input layer can comprise an assay input port extending from the first surface to the second surface and at least one pressure nodule extending from the second surface. An output layer can comprise a plurality of staging capillaries each having an inlet and an outlet. The output layer can further comprise a capillary plane disposed above the plurality of staging capillaries in fluid communication with the assay input port. The capillary plane can be sized to draw the assay from the assay input port to generally flood fill the plurality of staging capillaries.
摘要翻译：一种用于填充微板的填充装置。微量培养板具有多个孔，每个孔的大小用于接受测定。填充装置可以包括具有第一表面和相对的第二表面的测定输入层。测定输入层可以包括从第一表面延伸到第二表面的测定输入端口和从第二表面延伸的至少一个压力结节。输出层可以包括多个分级毛细管，每个具有入口和出口。输出层还可以包括设置在多个分级毛细管上方的毛细管平面，其与分析输入端口流体连通。毛细管平面的尺寸可以从测定输入端口抽取测定值，以通常灌注多个分期毛细血管。

2. 发明申请

US20090197277A1 High Density Plate Filler 审中-公开
标题翻译：高密度平板填料
公开(公告)号：US20090197277A1
公开(公告)日：2009-08-06
申请号：US12417536
申请日：2009-04-02
申请人： Nigel P. Beard , Albert L. Carrillo , Ian A. Harding , Mark T. Reed
发明人： Nigel P. Beard , Albert L. Carrillo , Ian A. Harding , Mark T. Reed
IPC分类号： C12Q1/68 , B01L3/02
CPC分类号： B01L3/50853 , B01L3/0293 , B01L3/5025 , B01L3/50851 , B01L3/563 , B01L2200/021 , B01L2200/025 , B01L2200/0605 , B01L2200/0642 , B01L2200/0684 , B01L2300/0829 , B01L2300/0864 , B01L2300/1805 , B01L2400/0406 , B01L2400/0409 , B01L2400/0487 , G01N35/1074
摘要： A filling apparatus for filling a microplate. The microplate having a plurality of wells each sized to receive an assay. The filling apparatus can comprise an assay input layer having a first surface and an opposing second surface. The assay input layer can comprise an assay input port extending from the first surface to the second surface and at least one pressure nodule extending from the second surface. An output layer can comprise a plurality of staging capillaries each having an inlet and an outlet. A intermediate layer can be disposed between the assay input layer and the output layer. The intermediate layer can be flexible and comprise a plurality of microfluidic channels and a flow aperture being in fluid communication with the assay input port and the plurality of microfluidic channels. The assay input layer can be moveable relative to the intermediate layer such that the at least one pressure nodule engages the intermediate layer to apply a clamping force on at least a portion of one of the plurality of microfluidic channels to reduce flow of the assay therethrough.
摘要翻译：一种用于填充微板的填充装置。微量培养板具有多个孔，每个孔的大小用于接受测定。填充装置可以包括具有第一表面和相对的第二表面的测定输入层。测定输入层可以包括从第一表面延伸到第二表面的测定输入端口和从第二表面延伸的至少一个压力结节。输出层可以包括多个具有入口和出口的分段毛细管。中间层可以设置在测定输入层和输出层之间。中间层可以是柔性的并且包括多个微流体通道，并且流动孔与测定输入端口和多个微流体通道流体连通。测定输入层可以相对于中间层移动，使得至少一个压力结节接合中间层，以在多个微流体通道之一的至少一部分上施加夹紧力，以减少测定的流动。

3. 发明授权

US08676383B2 Device for carrying out chemical or biological reactions 有权
标题翻译：用于进行化学或生物反应的装置
公开(公告)号：US08676383B2
公开(公告)日：2014-03-18
申请号：US11850345
申请日：2007-09-05
申请人： Lim Hi Tan , Jew Kwee Ngui , Hon Siu Shin , Ui Leng Soh , Yang Hooi Kee , Hock Lai Khoo , Mark T. Reed , Ching Ong Lau , Wolfgang Heimberg
发明人： Lim Hi Tan , Jew Kwee Ngui , Hon Siu Shin , Ui Leng Soh , Yang Hooi Kee , Hock Lai Khoo , Mark T. Reed , Ching Ong Lau , Wolfgang Heimberg
IPC分类号： G05B21/00
CPC分类号： B01L7/52 , B01L3/50855 , B01L7/54 , B01L2200/147 , B01L2300/0829 , B01L2300/1822 , B01L2300/1827 , B01L2300/1855 , C12Q1/686 , G01N21/6452
摘要： The invention relates to a device for carrying out of chemical or biological reactions with a reaction vessel receiving element for receiving a microtiter plate with several reaction vessels, wherein the reaction vessel receiving element has several recesses arranged in a regular pattern to receive the respective reaction vessels, a heating device for heating the reaction vessel receiving element, and a cooling device for cooling the reaction vessel. The invention is characterized by the fact that the reaction vessel receiving element is divided into several segments. The individual segments are thermally decoupled from one another, and each segment is assigned a heating device which may be actuated independently of the others. By means of the segmentation of the reaction vessel receiving element, it is possible for zones to be set and held at different temperatures. Because the reaction vessel receiving element is suitable for receiving standard microtiter plates, the device according to the invention may be integrated in existing process sequences.
摘要翻译：本发明涉及一种用于与反应容器接收元件进行化学或生物反应的装置，用于接收具有几个反应容器的微量滴定板，其中反应容器接收元件具有以规则图案排列以容纳相应的反应容器，用于加热反应容器接收元件的加热装置和用于冷却反应容器的冷却装置。本发明的特征在于将反应容器接收元件分成几段。各个片段彼此热分离，并且每个片段被分配加热装置，其可以独立于其它片段致动。通过反应容器接收元件的分割，可以将区域设定并保持在不同的温度。因为反应容器接收元件适于接收标准微量滴定板，所以根据本发明的装置可以集成在现有的工艺顺序中。

4. 发明申请

US20130344540A1 METHODS FOR MINIMIZING SEQUENCE SPECIFIC BIAS 审中-公开
标题翻译：最小化序列特定偏差的方法
公开(公告)号：US20130344540A1
公开(公告)日：2013-12-26
申请号：US13980302
申请日：2012-01-10
申请人： Mark T. Reed , Mark Pratt
发明人： Mark T. Reed , Mark Pratt
IPC分类号： C12P19/34
CPC分类号： C12P19/34 , C12Q1/6837 , C12Q1/6846 , C12Q2565/501
摘要： Provided herein are methods of nucleic acid amplification. An exemplary method includes the steps of (a) providing a surface comprising a plurality of patches of primers, (b) providing a plurality of different nucleic acid molecules, (c) contacting the plurality of different nucleic acid molecules with the surface under conditions wherein the nucleic acid molecules bind to primers at only a subset of the patches, (d) amplifying the nucleic acid molecules under conditions to saturate the subset of patches with copies of the nucleic acid molecules, and repeating steps (c) and (d), thereby increasing the number of patches that are saturated with copies of the nucleic acid molecules.
摘要翻译：本文提供了核酸扩增的方法。示例性方法包括以下步骤：（a）提供包含多个引物片的表面，（b）提供多个不同的核酸分子，（c）在多个不同的核酸分子的条件下使多个不同的核酸分子与表面接触，所述核酸分子仅在片段的一个子集上结合引物，（d）在使所述核酸分子拷贝饱和的条件下扩增核酸分子，并重复步骤（c）和（d），从而增加用核酸分子拷贝饱和的斑块的数量。

5. 发明授权

US07670834B2 Gas thermal cycler 有权
标题翻译：气体热循环仪
公开(公告)号：US07670834B2
公开(公告)日：2010-03-02
申请号：US11166958
申请日：2005-06-23
申请人： Adrian Fawcett , Mark T. Reed
发明人： Adrian Fawcett , Mark T. Reed
IPC分类号： C12M1/00 , C12M3/00
CPC分类号： B01L3/50855 , B01L3/50851 , B01L7/52 , B01L2300/0654 , B01L2300/1827 , B01L2300/1838 , B01L2300/1844 , B01L2300/1894 , F28F1/16
摘要： The present application relates to an apparatus and method for thermal cycling using a source of cooling gas.
摘要翻译：本申请涉及一种使用冷却气体源的热循环的装置和方法。

6. 发明授权

US07604937B2 Encoding and decoding reactions for determining target polynucleotides 失效
标题翻译：用于确定靶多核苷酸的编码和解码反应
公开(公告)号：US07604937B2
公开(公告)日：2009-10-20
申请号：US11090468
申请日：2005-03-24
申请人： Kai Qin Lao , Mark T. Reed
发明人： Kai Qin Lao , Mark T. Reed
IPC分类号： C12Q1/68 , C07H21/04
CPC分类号： C12Q1/6869 , C12Q1/6851 , C12Q2525/191 , C12Q2525/185 , C12Q2521/501 , C12Q2525/155 , C12Q2521/313 , C12Q2537/143 , C12Q2525/161
摘要： The present invention is directed to methods, reagents, and kits for detecting the presence or absence of (or quantifying) target polynucleotide sequences in at least one sample using encoding and decoding reactions. When a particular target polynucleotide is present in a sample, a reaction product is formed in the encoding reaction that includes addressable primer portions. At least one label and at least one address primer is employed in the decoding amplification reaction thereby providing a detectable signal value depending upon whether a sequence is present or absent. In some embodiments universal bases and reduced libraries of probes can be employed for highly multiplexed analysis of target polynucleotides.
摘要翻译：本发明涉及用于使用编码和解码反应检测至少一个样品中靶多核苷酸序列的存在或不存在（或定量）的方法，试剂和试剂盒。当特定靶多核苷酸存在于样品中时，在编码反应中形成包含可寻址引物部分的反应产物。在解码扩增反应中使用至少一个标签和至少一个地址引物，从而根据序列是否存在提供可检测的信号值。在一些实施方案中，探针的通用碱基和还原型文库可用于靶多核苷酸的高度多重分析。

7. 发明申请

US20080274511A1 DEVICE FOR CARRYING OUT CHEMICAL OR BIOLOGICAL REACTIONS 有权
标题翻译：执行化学或生物反应的装置
公开(公告)号：US20080274511A1
公开(公告)日：2008-11-06
申请号：US11850345
申请日：2007-09-05
申请人： Lim Hi TAN , Jew Kween Ngui , Hon Siu Shin , Ui Leng Soh , Yang Hooi Kee , Hock Lai Khoo , Mark T. Reed , Ching Ong Lau , Wolfgang Heimberg
发明人： Lim Hi TAN , Jew Kween Ngui , Hon Siu Shin , Ui Leng Soh , Yang Hooi Kee , Hock Lai Khoo , Mark T. Reed , Ching Ong Lau , Wolfgang Heimberg
IPC分类号： C12P19/34 , C12M1/02 , C12M1/34
CPC分类号： B01L7/52 , B01L3/50855 , B01L7/54 , B01L2200/147 , B01L2300/0829 , B01L2300/1822 , B01L2300/1827 , B01L2300/1855 , C12Q1/686 , G01N21/6452
摘要： The invention relates to a device for carrying out of chemical or biological reactions with a reaction vessel receiving element for receiving a microtiter plate with several reaction vessels, wherein the reaction vessel receiving element has several recesses arranged in a regular pattern to receive the respective reaction vessels, a heating device for heating the reaction vessel receiving element, and a cooling device for cooling the reaction vessel. The invention is characterized by the fact that the reaction vessel receiving element is divided into several segments. The individual segments are thermally decoupled from one another, and each segment is assigned a heating device which may be actuated independently of the others. By means of the segmentation of the reaction vessel receiving element, it is possible for zones to be set and held at different temperatures. Because the reaction vessel receiving element is suitable for receiving standard microtiter plates, the device according to the invention may be integrated in existing process sequences.
摘要翻译：本发明涉及一种用于与反应容器接收元件进行化学或生物反应的装置，用于接收具有几个反应容器的微量滴定板，其中反应容器接收元件具有以规则图案排列以容纳相应的反应容器，用于加热反应容器接收元件的加热装置和用于冷却反应容器的冷却装置。本发明的特征在于将反应容器接收元件分成几段。各个片段彼此热分离，并且每个片段被分配加热装置，其可以独立于其它片段致动。通过反应容器接收元件的分割，可以将区域设定并保持在不同的温度。因为反应容器接收元件适于接收标准微量滴定板，所以根据本发明的装置可以集成在现有的工艺顺序中。

8. 发明授权

US06338802B1 Multi-well microfiltration apparatus 有权
标题翻译：多孔微滤装置
公开(公告)号：US06338802B1
公开(公告)日：2002-01-15
申请号：US09565566
申请日：2000-05-04
申请人： Kevin S. Bodner , Alfred P. Madden , Joseph Jackson , Jason H. Halsey , Mark T. Reed , Ward Frye , Mark F. Oldham , Stephen E. Moring , Jon Hoshizaki
发明人： Kevin S. Bodner , Alfred P. Madden , Joseph Jackson , Jason H. Halsey , Mark T. Reed , Ward Frye , Mark F. Oldham , Stephen E. Moring , Jon Hoshizaki
IPC分类号： B01D6100
CPC分类号： B01L3/5025 , B01D61/18 , B01L3/50255 , B01L2400/049 , G01N35/0099 , G01N35/028 , G01N35/1074 , G01N2035/00485 , G01N2035/102 , Y10T436/25
摘要： The present invention provides multi-well plates and column arrays in which samples (e.g., cell lysates containing nucleic acids of interest, such as RNA) can be analyzed and/or processed. In one embodiment, the microfiltration arrangement is a multilayer structure, including (i) a column plate having an array of minicolumns into which samples can be placed, (ii) a discrete filter element disposed in each minicolumn, (iii) a drip-director plate having a corresponding array of drip directors through which filtrate may egress, and (iv) a receiving-well plate having a corresponding array of receiving wells into which filtrate can flow. The invention provides multi-well microfiltration arrangements that are relatively simple to manufacture and that overcome many of the problems associated with the prior arrangements relating to (i) cross-contamination due to wicking across a common filter sheet or (ii) individual filter elements entrapping sample constituents within substantial dead volumes. Further, the invention provides multi-well microfiltration arrangements that adequately support discrete filter elements disposed in the wells without creating substantial preferential flow. Additionally, the invention provides multi-well microfiltration arrangements that avoid cross-contamination due to aerosol formation, pendent drops and/or splattering. Other disclosed features of the invention provide for the automated covering or heat-sealing of filtrate samples separately collected in an array of wells.
摘要翻译：本发明提供了多孔板和柱阵列，其中可以分析和/或处理样品（例如，含有目标核酸的细胞裂解物，例如RNA）。在一个实施方案中，微滤装置是多层结构，包括（i）具有可以放置样品的小柱阵列的柱板，（ii）设置在每个小柱中的离散过滤元件，（iii）滴液导向器板具有相应的滴液导向器阵列，滤液可以通过其排出;以及（iv）接收孔板，其具有滤液能够流过的相应阵列的接收孔。本发明提供了相对简单的多孔微量过滤装置，并且克服了许多与先前布置有关的问题，这些问题涉及（i）由于芯毛穿过公共过滤片引起的交叉污染或（ii）截留的单个过滤器元件大量死体积的样品成分。此外，本发明提供了多孔微过滤装置，其充分地支撑设置在井中的分立的过滤元件，而不产生实质的优先流动。此外，本发明提供了多孔微滤装置，其避免了由于气溶胶形成，悬垂滴和/或飞溅而导致的交叉污染。本发明的其它公开的特征提供了分别收集在孔阵列中的滤液样品的自动覆盖或热封。

9. 发明授权

US09052298B2 High density plate filler 有权
标题翻译：高密度板填料
公开(公告)号：US09052298B2
公开(公告)日：2015-06-09
申请号：US13049686
申请日：2011-03-16
申请人： Mark T. Reed , Albert L. Carrillo , Ian A. Harding
发明人： Mark T. Reed , Albert L. Carrillo , Ian A. Harding
IPC分类号： B01L3/00 , G01N35/02 , G01N21/64 , B01L3/02 , B01L7/00 , B01L9/00 , G01N21/27
CPC分类号： G01N35/028 , B01L3/0293 , B01L3/5025 , B01L3/50851 , B01L3/50853 , B01L3/563 , B01L7/52 , B01L9/50 , B01L9/523 , B01L2200/021 , B01L2200/025 , B01L2200/0605 , B01L2200/0642 , B01L2200/0689 , B01L2300/022 , B01L2300/044 , B01L2300/046 , B01L2300/0829 , B01L2300/0851 , B01L2300/0864 , B01L2300/1805 , B01L2400/0406 , B01L2400/0409 , B01L2400/0487 , G01N21/274 , G01N21/6452
摘要： A filling apparatus for filling a microplate. The microplate can comprise a plurality of wells each sized to receive an assay. A substrate can comprise a first surface and an opposing second surface, a first assay input port for receiving the assay disposed on the first surface, a plurality of staging capillaries extending through the substrate, and a first plurality of microfluidic channels fluidly coupling the first assay input port with at least one of the plurality of staging capillaries. Each of the plurality of staging capillaries can comprise an inlet and an outlet and be sized to receive the assay.
摘要翻译：一种用于填充微板的填充装置。微量培养板可以包括多个孔，每个孔的尺寸适于接受测定。基底可以包括第一表面和相对的第二表面，用于接收设置在第一表面上的测定的第一测定输入端口，延伸穿过基底的多个分期毛细血管，以及第一多个微流体通道，其将第一测定输入端口与多个分段毛细管中的至少一个。多个分级毛细管中的每一个可以包括入口和出口，并且其大小适于接收测定。

10. 发明授权

US07998435B2 High density plate filler 有权
标题翻译：高密度板填料
公开(公告)号：US07998435B2
公开(公告)日：2011-08-16
申请号：US11393061
申请日：2006-03-30
申请人： Mark T. Reed
发明人： Mark T. Reed
IPC分类号： B01L3/00
CPC分类号： G01N35/1074 , B01L3/0293 , B01L3/5025 , B01L3/50851 , B01L3/50853 , B01L3/563 , B01L2200/021 , B01L2200/025 , B01L2200/0605 , B01L2200/0642 , B01L2200/0684 , B01L2300/0829 , B01L2300/0864 , B01L2300/1805 , B01L2400/0406 , B01L2400/0409 , B01L2400/0487 , Y10T436/2575
摘要： A filling apparatus for filling a microplate. The microplate having a plurality of wells each sized to receive an assay. The filling apparatus can comprise an output layer having a plurality of capillaries, wherein a first grouping of the capillaries is separated from a second grouping by a hydrophobic feature. Each of the plurality of capillaries can comprise an inlet and an outlet. A funnel assembly can comprise a funnel member sized to receive the assay. The funnel member can comprise an outlet for delivering a fluid bead of the assay along a top surface of the output layer and in fluid communication with each of the plurality of capillaries such that a portion of the fluid bead can be drawn within at least some of the plurality of capillaries in response to capillary force. The funnel assembly and the output layer can be moveable relative to each other between a first position and a second position to draw the fluid bead across the top surface.
摘要翻译：一种用于填充微板的填充装置。微量培养板具有多个孔，每个孔的大小用于接受测定。填充设备可以包括具有多个毛细管的输出层，其中毛细管的第一组通过疏水特征与第二组分离。多个毛细管中的每一个可以包括入口和出口。漏斗组件可以包括尺寸适于接收测定的漏斗构件。漏斗构件可以包括用于沿着输出层的顶表面递送测定的流体珠的出口，并且与多个毛细管中的每一个流体连通，使得流体珠的一部分可以在至少一些多个毛细管响应于毛细管力。漏斗组件和输出层可以在第一位置和第二位置之间相对于彼此移动，以便在顶表面上拉动流体珠。

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