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1. 发明申请

US20060172339A1 Particle-based multiplex assay for identifying glycosylation 审中-公开
标题翻译：用于鉴定糖基化的基于粒子的多重测定
公开(公告)号：US20060172339A1
公开(公告)日：2006-08-03
申请号：US11289060
申请日：2005-11-29
申请人： Wayne Patton , Mack Schermer , Mark Bobrow
发明人： Wayne Patton , Mack Schermer , Mark Bobrow
IPC分类号： G01N33/53 , G06F19/00
CPC分类号： G01N33/585 , G01N33/6842 , G01N2400/00 , G01N2400/10 , G01N2400/40 , G01N2400/50 , G01N2405/10
摘要： The present invention provides systems, methods and kits for performing multiplexed assays for glycoproteins using encoded particles as supports for glycoprotein specific binding agents.
摘要翻译：本发明提供用于使用编码的颗粒作为糖蛋白特异性结合剂的载体进行糖蛋白多重测定的系统，方法和试剂盒。

2. 发明申请

US20060177850A1 Particle-based multiplex assay system with three or more assay reporters 审中-公开
标题翻译：具有三个或更多测定记录的基于粒子的多重测定系统
公开(公告)号：US20060177850A1
公开(公告)日：2006-08-10
申请号：US11321374
申请日：2005-12-29
申请人： Mack Schermer , Mark Bobrow , Wayne Patton
发明人： Mack Schermer , Mark Bobrow , Wayne Patton
IPC分类号： C12Q1/68 , C12M1/34 , G01N33/551
CPC分类号： G01N33/54313 , B01J19/0046 , B01J2219/005 , B01J2219/00502 , B01J2219/00504 , B01J2219/00545 , B01J2219/00547 , B01J2219/00576 , B01J2219/00592 , B01J2219/00596 , B01J2219/00605 , B01J2219/00612 , B01J2219/00621 , B01J2219/00702 , B01J2219/00722 , C12Q1/6827 , G01N33/54333 , G01N33/54366 , G01N2035/00772 , C12Q2565/102 , C12Q2563/107 , C12Q2537/143 , C12Q2563/149
摘要： A system and method for developing and utilizing particle-based n-multiplexed assays that include three or more reporters utilizes n particle sets that are associated with particle identification images or labels (IDs) that differ between sets. The encoded particles for a given set are coated with a specific binding member, or in the case of the sandwich assay with coupled capture and detector binding pair members, to form particle types. The sets of particle types are then pooled, and aliquots of the particle types are removed to assay vessels. Next, samples with three or four reporter molecules are supplied to the respective vessels. After one or more incubation periods, the particles are supplied to a reader system, which determines the particle IDs to identify the particle types and also detects the reporter signals. The reader system includes multiple excitation lasers that excite the various reporters in sequence or in parallel, to supply associated signals to one or more detectors. Emission filters and wavelength discriminators are included such that a given detector receives at a given time the signals associated with a single assay binding label. The system further develops greater capacity sandwich assays by assigning subsets of capture and detector antibody pairings to the three or four reporters, respectively. The system performs greater numbers of differential RNA expressions based on the use of the three or more reporters, with one or more reporters assigned to the reference sample and the other reporters assigned to respective test samples. The system and method are also capable of performing greater numbers of SNPs utilizing primer extension reactions, by assigning different color reporters to the respective nucleotides or terminators.
摘要翻译：用于开发和利用包括三个或更多个记录器的基于粒子的n多元化测定的系统和方法利用与在组之间不同的粒子识别图像或标签（ID）相关联的n个粒子集。给定组的编码颗粒用特异性结合成员涂覆，或者在使用偶联捕获和检测器结合对成员的夹心测定的情况下，形成颗粒类型。然后将这些颗粒类型集合，并将等分的颗粒类型移除到测定容器中。接下来，将具有三个或四个报告分子的样品供应到各个容器。在一个或多个孵育期后，颗粒被提供给读取器系统，其确定颗粒ID以识别颗粒类型并且还检测报告信号。读取器系统包括多个激发激光器，其按顺序或并联激励各种报告器，以将相关信号提供给一个或多个检测器。包括发射滤波器和波长鉴别器，使得给定检测器在给定时间接收与单个测定结合标签相关联的信号。该系统通过分别将三个或四个记录者的捕获和检测抗体配对分组进一步开发更大容量的夹心测定。该系统基于使用三个或更多个记者，分配给参考样本的一个或多个记者和分配给各个测试样品的其他记者，来执行更多数量的差异RNA表达。通过将不同的颜色记录分配给各个核苷酸或终止子，该系统和方法还能够利用引物延伸反应来进行更多数量的SNP。

3. 发明申请

US20070166810A1 METHODS AND COMPOSITIONS FOR DETECTING ENZYMATIC ACTIVITY 审中-公开
标题翻译：用于检测酶活性的方法和组合物
公开(公告)号：US20070166810A1
公开(公告)日：2007-07-19
申请号：US11615733
申请日：2006-12-22
申请人： Mark Bobrow , Karl Adler , Mack Schermer
发明人： Mark Bobrow , Karl Adler , Mack Schermer
IPC分类号： C12N9/00
CPC分类号： C12Q1/34 , C12Q1/00 , G01N33/585 , G01N2333/98 , G01N2800/04
摘要： This invention relates to methods and compositions useful in detecting enzymatic activity. Also featured are methods for diagnosing enzyme deficiencies such as metabolic disorders.
摘要翻译：本发明涉及可用于检测酶活性的方法和组合物。还有一些诊断酶缺乏症如代谢紊乱的方法。

4. 发明申请

US20070166739A1 COMPARATIVE GENOMIC HYBRIDIZATION ON ENCODED MULTIPLEX PARTICLES 审中-公开
标题翻译：编码多重粒子的比较基因组杂交
公开(公告)号：US20070166739A1
公开(公告)日：2007-07-19
申请号：US11615739
申请日：2006-12-22
申请人： Mark Bobrow , Karl Adler , Mack Schermer
发明人： Mark Bobrow , Karl Adler , Mack Schermer
IPC分类号： C12Q1/68 , C12M3/00
CPC分类号： C12Q1/6809 , C12Q1/6834 , C12Q1/6841 , C12Q2565/518 , C12Q2563/149
摘要： Disclosed herein are methods and compositions for evaluating genomic content using encoded particles to evaluate multiple samples in parallel.
摘要翻译：本文公开了使用编码的颗粒来评估基因组含量以平行评估多个样品的方法和组合物。

5. 发明申请

US20060228735A1 Multiplex assay systems 审中-公开
标题翻译：多重测定系统
公开(公告)号：US20060228735A1
公开(公告)日：2006-10-12
申请号：US11394673
申请日：2006-03-31
申请人： Mark Bobrow , Mack Schermer
发明人： Mark Bobrow , Mack Schermer
IPC分类号： C12Q1/68 , C12M1/34
CPC分类号： C12Q1/6818 , C12Q1/6834 , G01N33/585 , C12Q2565/537 , C12Q2563/149 , C12Q2563/103 , C12Q2565/1015 , C12Q2565/525 , C12Q2537/143 , C12Q2525/301 , C12Q2565/101 , C12Q2525/186 , C12Q2525/161 , C12Q2537/1373 , C12Q2533/101
摘要： The methods described herein can be used, e.g., for detecting at least two target molecules in a sample (e.g., a single sample). Exemplary methods include using a target-specific switch probe attached to a substrate and using zip codes to associate holographically encoded particles with a target-specific probe.
摘要翻译：本文描述的方法可以用于例如检测样品中的至少两个靶分子（例如单个样品）。示例性的方法包括使用连接到基板的目标特异性开关探针并使用邮政编码将全息编码的粒子与目标特异性探针相关联。

6. 发明申请

US20050272046A1 Methods, apparatus and compositions for improved measurements with optical biosensors 失效
标题翻译：用于利用光学生物传感器改进测量的方法，装置和组合物
公开(公告)号：US20050272046A1
公开(公告)日：2005-12-08
申请号：US10864086
申请日：2004-06-09
申请人： Mack Schermer , Mark Bobrow , Philip Buzby , Thomas Mullinax
发明人： Mack Schermer , Mark Bobrow , Philip Buzby , Thomas Mullinax
IPC分类号： C12M1/32 , C12M1/34 , C12Q1/68 , G01N21/25
CPC分类号： G01N21/253 , B01L9/50 , G01N21/7743
摘要： The invention provides an apparatus for interrogating an optical biosensor. The apparatus includes (a) locating means for positioning one or more optical biosensors; (b) calibration means for generating a reference measurement; (c) one or more biosensors for generating one or more sample measurements, the biosensors being substantially planar and removable from the apparatus; (d) a reading means for interrogating biosensor and calibration means, and (e) a transport means for moving one or more of a biosensor, the calibration means and the reading means, whereby the reading means is positioned to interrogate the biosensor and the calibration means.
摘要翻译：本发明提供一种询问光学生物传感器的装置。该装置包括（a）用于定位一个或多个光学生物传感器的定位装置; （b）用于产生参考测量的校准装置; （c）用于产生一个或多个样品测量的一个或多个生物传感器，所述生物传感器基本上是平面的并且可从所述设备移除; （d）用于询问生物传感器和校准装置的读取装置，以及（e）用于移动生物传感器，校准装置和读取装置中的一个或多个的传送装置，由此读取装置被定位成询问生物传感器和校准手段。

7. 发明申请

US20070166835A1 MULTIPLEX ASSAYS USING MAGNETIC AND NON-MAGNETIC PARTICLES 审中-公开
标题翻译：使用磁性和非磁性颗粒的多重测定
公开(公告)号：US20070166835A1
公开(公告)日：2007-07-19
申请号：US11615731
申请日：2006-12-22
申请人： Mark Bobrow , Karl Adler , Mack Schermer
发明人： Mark Bobrow , Karl Adler , Mack Schermer
IPC分类号： G01N1/00
CPC分类号： C12Q1/6834 , G01N33/54313 , G01N33/54326 , G01N35/0098 , G01N35/028 , G01N2035/00564 , Y10T436/25 , C12Q2563/149 , C12Q2563/143 , C12Q2537/143
摘要： Disclosed herein are methods and compositions for multiplexed assays using magnetic and non-magnetic particles. Also disclosed are methods and compositions for performing multi-step assays within a single assay vessel.
摘要翻译：本文公开了使用磁性和非磁性颗粒的多重测定法的方法和组合物。还公开了用于在单个测定容器内进行多步测定的方法和组合物。

8. 发明申请

US20050269267A1 Separations platform based upon electroosmosis-driven planar chromatography 审中-公开
标题翻译：基于电渗雾驱动的平面色谱分离平台
公开(公告)号：US20050269267A1
公开(公告)日：2005-12-08
申请号：US11084501
申请日：2005-03-18
申请人： Wayne Patton , Mack Schermer
发明人： Wayne Patton , Mack Schermer
IPC分类号： B01D15/08 , C07K1/26 , G01N27/447
CPC分类号： C07K1/26 , C07K1/285 , G01N27/44747 , G01N30/58 , G01N30/92 , G01N2030/8813 , G01N2030/945
摘要： The present invention describes a system and method for separation of proteins, peptides and glycans by one-dimensional or two-dimensional electroosmosis-driven planar chromatography. Separation is performed using amphiphilic polymeric membranes, amphiphilic thin-layer chromatography plates or other planar amphiphilic surfaces as the stationary phase with a combination of organic and/or aqueous buffers as the mobile phase. Systematic selection of stationary phase supports, mobile phase buffers and operating conditions allow for the adaptation of the invention to a broad range of applications in proteomics, mass spectrometry, drug discovery and the pharmaceutical sciences.
摘要翻译：本发明描述了通过一维或二维电渗驱动的平面色谱分离蛋白质，肽和聚糖的系统和方法。使用两亲聚合物膜，两亲性薄层色谱板或其它平面两亲性表面作为固定相进行分离，其中有机和/或含水缓冲液的组合作为流动相。固定相载体，流动相缓冲液和操作条件的系统选择允许本发明适用于蛋白质组学，质谱，药物发现和药学科学领域的广泛应用。

9. 发明申请

US20050181510A1 Method and apparatus for automatic pin detection in microarray spotting instruments 有权
公开(公告)号：US20050181510A1
公开(公告)日：2005-08-18
申请号：US11103162
申请日：2005-04-11
申请人： Mack Schermer , C. Candiloro
发明人： Mack Schermer , C. Candiloro
IPC分类号： G01N33/53 , B01J19/00 , B01L3/02 , C40B60/14 , G01N37/00 , G01N35/00
CPC分类号： B82Y30/00 , B01J19/0046 , B01J2219/00387 , B01J2219/00527 , B01J2219/00585 , B01J2219/0059 , B01J2219/00596 , B01J2219/00605 , B01J2219/00612 , B01J2219/00621 , B01J2219/00637 , B01J2219/00659 , B01J2219/00677 , B01J2219/00689 , B01J2219/00693 , B01L3/0241 , B01L3/0244 , C40B60/14 , Y10T436/11 , Y10T436/114998 , Y10T436/119163 , Y10T436/2575
摘要： A method and apparatus are provided for automatically sensing the presence (or absence) of spot dispensers such as pins in various possible mounting locations in the printhead of a microarray spotting instrument. Pin-location data obtained by the method and apparatus is provided to the computer controller of the instrument, which uses the data to control the motion of the printhead during operation of the instrument. A pin detection apparatus includes one or more sensor elements that automatically sense possible pin locations in the printhead for the presence of pins. The sensor elements are preferably arranged in an array corresponding to the array of pin locations in the printhead so that pin detection at each pin location can be performed simultaneously. Various types of sensor elements can be used to locate pins in a printhead. For example, sensors can be used that are remote from or attached to a printhead. Pin sensors can be used that sense pin locations from various positions relative to the printhead including above or below the printhead. Also, pin sensing can be by contact or non-contact mechanisms. The method and apparatus allow pin positions in a printhead to be quickly and accurately determined, and without significant risk of damaging the pins, which are fragile.

10. 发明申请

US20050213101A1 Plasmon resonance measuring method and apparatus 失效
标题翻译：等离子体共振测量方法和装置
公开(公告)号：US20050213101A1
公开(公告)日：2005-09-29
申请号：US11090583
申请日：2005-03-25
申请人： Mack Schermer
发明人： Mack Schermer
IPC分类号： B01L3/00 , G01N21/25 , G01N21/55
CPC分类号： B01L3/5085 , B01L2300/0654 , B01L2300/0829 , B01L2300/0851 , G01N21/253 , G01N21/553
摘要： Detection apparatus for performing surface plasmon resonance (SPR) measurements on a sample includes a support having side walls and a bottom wall defining a plurality of adjacent wells. The bottom wall has integrated therein SPR detectors underlying the wells for detecting the refractive indexes of samples deposited in the wells. Each SPR detector includes a prism having an exterior entrance surface, an exterior exit facet and an interior sensing surface located at the bottom of the overlying well, and a thin metal layer coated on the sensing surface. The prism may be molded from a transparent polymer into the bottom of a standard-format microplate structure. That structure may be used in conjunction with an SPR measuring or reading instrument to perform label-less assay measurements on various samples in an efficient and reliable manner. A method of acquiring the assay measurements is also disclosed.
摘要翻译：用于对样品进行表面等离子体共振（SPR）测量的检测装置包括具有侧壁和限定多个相邻孔的底壁的支撑件。底壁已经集成有井下的SPR检测器，用于检测沉积在孔中的样品的折射率。每个SPR检测器包括具有外部入口表面的棱镜，外部出口小面和位于上覆井的底部的内部感测表面，以及涂覆在感测表面上的薄金属层。棱镜可以由透明聚合物模制成标准格式的微板结构的底部。该结构可以与SPR测量或阅读仪器结合使用，以高效和可靠的方式对各种样品进行无标签测定。还公开了获取测定测量的方法。

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