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1. 发明申请

US20150299729A1 ULTRAHIGH THROUGHPUT MICROINJECTION DEVICE 有权
公开(公告)号：US20150299729A1
公开(公告)日：2015-10-22
申请号：US14379486
申请日：2013-02-21
申请人： Indiana University Research and Technology Corporation , The Regents of the University of California
发明人： Chris Ballas , Masa Rao , Yanyan Zhang
IPC分类号： C12N15/89 , B01J19/00
CPC分类号： C12N15/89 , B01J19/0046 , B01J2219/00452 , B01J2219/00502 , B01J2219/00587 , B01J2219/00743 , C12M23/12
摘要： Many applications in cell biology, genetic engineering, cell-based therapeutics, and drug discovery require precise and safe methods for introducing membrane-impermeable molecules into cells. This can be implemented satisfactorily by microinjection. However, disadvantages of traditional manual microinjection include high degree of operator skill, low throughput and labor-intensiveness. Many studies have focused on developing automated and high-throughput systems for microinjection to address these limitations. However, none have provided sufficient throughput for applications such as ex vivo cell therapy, where manipulation of many cells is helpful.
摘要翻译：在细胞生物学，基因工程，基于细胞的治疗和药物发现中的许多应用需要精确和安全的将膜不可渗透分子引入细胞的方法。这可以通过显微注射令人满意地实现。然而，传统的手动显微注射的缺点包括操作员技能程度高，生产能力低和劳动强度大。许多研究集中在开发用于显微注射的自动化和高通量系统来解决这些局限性。然而，没有一个为诸如离体细胞治疗的应用提供足够的通量，其中许多细胞的操作是有帮助的。

2. 发明申请

US20150162141A1 METHOD FOR PRODUCING MICROCARRIERS 有权
标题翻译：生产微孔板的方法
公开(公告)号：US20150162141A1
公开(公告)日：2015-06-11
申请号：US14414174
申请日：2013-07-22
申请人： MYCARTIS NV
发明人： Raphael Tornay , Nicolas Demierre , Stephan Gamper , Philippe Renaud
IPC分类号： H01G13/06
CPC分类号： H01G13/06 , B01J2219/005 , B01J2219/00502 , B01J2219/00536 , B01J2219/00556 , B01J2219/00637 , B01L3/502707 , B01L3/502761 , B01L3/508 , B01L3/54 , B01L3/545 , B01L2200/0647 , B01L2200/10 , B01L2200/12 , B01L2300/0803 , B01L2300/0819 , B01L2300/0851 , B01L2300/0893 , B81B2201/058 , B81B2201/06 , B81C99/008 , G01N33/54313
摘要： The present invention relates to a method for producing microcarriers comprising the following steps: (a) providing a wafer having a sandwich structure comprising a bottom layer, a top layer and an insulating layer located between said bottom and top layers, (b) etching away the top layer to delineate lateral walls of bodies of the microcarriers, (c) depositing a first active layer at least on a top surface of the bodies, (d) applying a continuous polymer layer over the first active layer, (e) etching away the bottom layer and the insulating layer, (f) removing the polymer layer to release the microcarriers.
摘要翻译：本发明涉及一种生产微载体的方法，包括以下步骤：（a）提供具有夹层结构的晶片，该夹层结构包括位于所述底层和顶层之间的底层，顶层和绝缘层，（b）蚀刻掉描绘微载体的主体的侧壁的顶层，（c）至少在主体的顶表面上沉积第一有源层，（d）在第一有源层上施加连续聚合物层，（e）蚀刻掉底层和绝缘层，（f）去除聚合物层以释放微载体。

3. 发明申请

US20110183272A1 METHOD FOR FABRICATING MICROBEADS AND MICROBEADS 有权
标题翻译：制备微生物和微生物的方法
公开(公告)号：US20110183272A1
公开(公告)日：2011-07-28
申请号：US13120967
申请日：2009-09-04
申请人： Mari Ichimura , Kenzo Machida , Noriyuki Kishii , Masannobu Tanaka
发明人： Mari Ichimura , Kenzo Machida , Noriyuki Kishii , Masannobu Tanaka
IPC分类号： G03F7/20
CPC分类号： G01N33/54313 , B01J2219/00432 , B01J2219/005 , B01J2219/00502 , B01J2219/0061 , B01J2219/00612 , B01J2219/00637 , B01J2219/00677 , B01J2219/00722
摘要： In one example embodiment, a method fabricates microbeads, which can supply a bead set containing a various types of microbeads and having distinct populations of the respective types of microbeads. In one example embodiment, the method includes forming a hydrophilic layer made of a hydrophilic organic material on a substrate. In one example embodiment, the method includes laminating on the hydrophilic layer a thin film capable of being peeled off in the form of microbeads. In one example embodiment, the method includes forming the thin film in a given configuration by photolithography. In one example embodiment, the method includes solid-phasing a given substance on the post-formed thin films. In one example embodiment, the method includes peeling off the post-formed thin films, which have been solid-phased with the substance, from the substrate along with at least a part of the hydrophilic layer to obtain microbeads.
摘要翻译：在一个示例性实施例中，一种制造微珠的方法，其可以提供包含各种类型的微珠并且具有各种类型的微珠的不同种群的珠粒组。在一个示例性实施例中，该方法包括在基底上形成由亲水性有机材料制成的亲水层。在一个示例性实施例中，该方法包括在亲水层上层叠能够以微珠形式剥离的薄膜。在一个示例性实施例中，该方法包括通过光刻法以给定的配置形成薄膜。在一个示例性实施例中，该方法包括将定影物质固定在后成形薄膜上。在一个示例性实施例中，该方法包括将已经与物质固相化的后成形薄膜与至少一部分亲水层一起从衬底剥离以获得微珠。

4. 发明申请

US20100260984A1 MICRODEVICES CONTAINING PHOTORECOGNIZABLE CODING PATTERNS AND METHODS OF USING AND PRODUCING THE SAME 审中-公开
标题翻译：包含可光化编码图案的微型装置及其使用和生产方法
公开(公告)号：US20100260984A1
公开(公告)日：2010-10-14
申请号：US12760379
申请日：2010-04-14
申请人： Lei WU , Xiaobo Wang , Guoliang Tao , Junquan Xu , Jing Cheng , Mingxian Huang , Baoquan Sun , Wei Shao , Litian Liu , Depu Chen , David M. Rothwarf , Weiping Yang
发明人： Lei WU , Xiaobo Wang , Guoliang Tao , Junquan Xu , Jing Cheng , Mingxian Huang , Baoquan Sun , Wei Shao , Litian Liu , Depu Chen , David M. Rothwarf , Weiping Yang
IPC分类号： B32B3/00
CPC分类号： C40B40/12 , B01J19/0046 , B01J2219/00457 , B01J2219/00468 , B01J2219/00497 , B01J2219/005 , B01J2219/00502 , B01J2219/0054 , B01J2219/00542 , B01J2219/00547 , B01J2219/00549 , B01J2219/00556 , B01J2219/00576 , B01J2219/00585 , B01J2219/0059 , B01J2219/00592 , B01J2219/00596 , B01J2219/00655 , B01J2219/00659 , B01J2219/00702 , B01J2219/00707 , B01J2219/0072 , B01J2219/00722 , B01J2219/00725 , B01J2219/00729 , B01J2219/00731 , B01J2219/00734 , B01J2219/0074 , B01J2219/00743 , B01L3/5027 , B01L3/50273 , B01L3/502761 , B01L2200/0647 , B01L2300/0819 , B01L2400/0415 , B01L2400/043 , B03C5/028 , B82Y5/00 , B82Y10/00 , B82Y30/00 , C07B2200/11 , C07K1/047 , C40B40/06 , C40B40/10 , G01N33/54366 , G01N33/54386 , G06K19/06009 , G06K19/06046 , G06K19/06196 , Y10T428/24917
摘要： This invention relates generally to the field of moiety or molecule analysis, isolation, detection and manipulation and library synthesis. In particular, the invention provides a microdevice, which microdevice comprises: a) a substrate; and b) a photorecognizable coding pattern on said substrate. Preferably, the microdevice does not comprise an anodized metal surface layer. Methods and kits for isolating, detecting and manipulating moieties, and synthesizing libraries using the microdevices are also provided. The invention further provides two-dimensional optical encoders and uses thereof. In certain embodiments, the invention provides a microdevice, which microdevice comprises: a) a magnetizable substance; and b) a photorecognizable coding pattern, wherein said microdevice has a preferential axis of magnetization. Systems and methods for isolating, detecting and manipulating moieties and synthesizing libraries using the microdevices are also provided.
摘要翻译：本发明一般涉及部分或分子分析，分离，检测和操作以及文库合成领域。特别地，本发明提供了一种微型器件，该微型器件包括：a）衬底; 和b）在所述衬底上的可识别的编码图案。优选地，微器件不包括阳极化金属表面层。还提供了用于分离，检测和操作部分以及使用微器件合成文库的方法和试剂盒。本发明还提供二维光学编码器及其用途。在某些实施方案中，本发明提供一种微型装置，该微型装置包括：a）可磁化物质; 以及b）可识别的编码图案，其中所述微器件具有优先磁化轴。还提供了用于使用微器件分离，检测和操作部分和合成文库的系统和方法。

5. 发明申请

US20090149340A1 Encoded microparticles 有权
标题翻译：编码微粒
公开(公告)号：US20090149340A1
公开(公告)日：2009-06-11
申请号：US12215607
申请日：2008-06-25
申请人： Randall J. True
发明人： Randall J. True
IPC分类号： C40B40/00 , C12N11/00 , C12N9/96 , C40B30/00 , C40B30/04 , G06K7/00
CPC分类号： G01N33/54313 , B01J2219/005 , B01J2219/00502 , B01J2219/00513 , B01J2219/00527 , B01J2219/00547 , B01J2219/00549 , B01J2219/00558 , B01J2219/0056 , G01N33/54366 , G01N2021/6441 , Y10T428/2982 , Y10T428/2991
摘要： Microparticles including spatially coded microparticles, systems for imaging and methods of detecting such microparticles as well as using the same in bioassays are provided.
摘要翻译：提供了包括空间编码的微粒的微粒，用于成像的系统和检测这种微粒的方法以及在生物测定中使用微粒。

6. 发明申请

US20060046245A1 Porous device 审中-公开
标题翻译：多孔装置
公开(公告)号：US20060046245A1
公开(公告)日：2006-03-02
申请号：US11240386
申请日：2005-10-03
申请人： Ryszard Kobylecki
发明人： Ryszard Kobylecki
IPC分类号： C40B60/08 , C12Q1/00 , C12P21/06
CPC分类号： C07K1/047 , B01J19/0046 , B01J2219/00454 , B01J2219/00497 , B01J2219/005 , B01J2219/00502 , B01J2219/0054 , B01J2219/00585 , B01J2219/00592 , B01J2219/00596 , B01J2219/00709 , B01J2219/0072 , B01J2219/00725 , C07B61/00 , C07B2200/11 , C40B40/10 , C40B50/14 , C40B70/00
摘要： A porous device for use in a method of synthesis comprising a body having an internal region which is porous wherein an active material, for example a solid support resin, is entrapped within the internal region.
摘要翻译：一种用于合成方法的多孔装置，其包括具有多孔的内部区域的主体，其中活性材料例如固体支撑树脂被截留在内部区域内。

7. 发明申请

US20040241701A1 Method for generation of modular polynucleotides using solid supports 审中-公开
标题翻译：使用固体支持物生成模块化多核苷酸的方法
公开(公告)号：US20040241701A1
公开(公告)日：2004-12-02
申请号：US10494996
申请日：2004-05-07
发明人： Vaughn Smider
IPC分类号： C12Q001/68 , G01N033/53
CPC分类号： C12N15/1093 , B01J19/0046 , B01J2219/00315 , B01J2219/00454 , B01J2219/00497 , B01J2219/005 , B01J2219/00502 , B01J2219/00504 , B01J2219/00511 , B01J2219/00527 , B01J2219/00585 , B01J2219/00599 , B01J2219/00605 , B01J2219/0061 , B01J2219/00612 , B01J2219/00619 , B01J2219/00621 , B01J2219/00626 , B01J2219/0063 , B01J2219/00637 , B01J2219/00641 , B01J2219/00644 , B01J2219/00675 , B01J2219/00722 , B01J2219/00725 , B82Y30/00 , C07B2200/11 , C07K16/00 , C12N15/1027 , C12P19/34 , C40B40/00
摘要： The invention generally relates to nucleic acid cloning and genetic engineering. The invention also relates to the field of molecular evolution and protein engineering.
摘要翻译：本发明一般涉及核酸克隆和遗传工程。本发明还涉及分子进化和蛋白质工程领域。

8. 发明申请

US20040115680A1 Biochemical method and apparatus for detecting genetic characteristic 审中-公开
标题翻译：用于检测遗传特征的生物化学方法和仪器
公开(公告)号：US20040115680A1
公开(公告)日：2004-06-17
申请号：US10467891
申请日：2004-01-28
发明人： Caroline Garey , Jodie Hadley , Mark England
IPC分类号： C12Q001/68 , G06F019/00 , G01N033/48 , G01N033/50
CPC分类号： G01N33/54313 , B01J19/0046 , B01J2219/005 , B01J2219/00502 , B01J2219/0054 , B01J2219/00547 , B01J2219/00596 , B01J2219/00605 , B01J2219/00689 , B01J2219/00702 , B01J2219/0072 , B01J2219/00722 , B01J2219/00729 , C40B40/06 , C40B70/00 , G01N33/54366 , G01N2500/00
摘要： There is describe a biochemical method for detecting one or more genetic characteristics. The method utilizes supports (1), wherein the largest dimension (3) of each support (1) is less than 250 um and wherein each support (1) incorporates sequential identification means (2). The method is distinguished in that it includes the steps of: attaching an information molecule (7), which is capable of interacting with at least one of said one or more genetic characteristic to be detected, to a main surface (11) of a support (1); suspending supports (1) comprising one or more different sequential identifications means (2) and one or more different information molecules (7) in a fluid; adding a sample (8) to be analysed to the fluid; detecting interaction signals from supports (1) in the fluid using signal detecting means (40); and reading the sequential identification means (2) of the supports (1) which have an interaction signal using reading means (3), thereby detecting at least one of said one or more genetic characteristic (8). There is also described apparatus susceptible for use in executing the above method.
摘要翻译：描述了用于检测一种或多种遗传特征的生物化学方法。该方法利用支撑件（1），其中每个支撑件（1）的最大尺寸（3）小于250um，并且其中每个支撑件（1）包括顺序识别装置（2）。该方法的区别在于，其包括以下步骤：将能够与要检测的所述一个或多个遗传特征中的至少一个相互作用的信息分子（7）附着到支撑体的主表面（11）（1）; 在流体中包括一个或多个不同顺序识别装置（2）和一个或多个不同信息分子（7）的悬浮支撑件（1）将待分析的样品（8）加入到流体中; 使用信号检测装置（40）检测来自流体中的支撑件（1）的相互作用信号; 以及使用读取装置（3）读取具有交互信号的支撑件（1）的顺序识别装置（2），从而检测所述一个或多个遗传特征（8）中的至少一个。还描述了易于用于执行上述方法的装置。

9. 发明申请

US20040058391A1 Three-dimensional array of supports for solid-phase parallel synthesis and method of use 审中-公开
标题翻译：用于固相平行合成的三维支撑阵列及其使用方法
公开(公告)号：US20040058391A1
公开(公告)日：2004-03-25
申请号：US10671341
申请日：2003-09-25
申请人： Aventis Pharmaceuticals Inc.
发明人： Stephen A. Baum
IPC分类号： G01N033/53 , C12M001/34
CPC分类号： B82Y30/00 , B01J19/0046 , B01J2219/00286 , B01J2219/0031 , B01J2219/00317 , B01J2219/00319 , B01J2219/00322 , B01J2219/00461 , B01J2219/00468 , B01J2219/00475 , B01J2219/00497 , B01J2219/005 , B01J2219/00502 , B01J2219/00522 , B01J2219/00527 , B01J2219/00585 , B01J2219/0059 , B01J2219/00596 , B01J2219/00668 , B01J2219/00675 , B01J2219/00689 , B01J2219/0072 , B01J2219/00722 , B01J2219/00725 , B01L2300/0819 , B01L2300/0829 , C40B40/06 , C40B40/10 , C40B50/14 , C40B60/14 , G01N33/543
摘要： A three-dimensional (3D) array of solid-phase supports is adapted to provide parallel synthesis of a library of molecules with 3D diversity. Individual locations in the 3D array may be assigned to selected molecules in the library such that molecules may be synthesized at and retrieved from such locations. Also, the supports include aperture walls in stacked plates; the supports may be suspended within stacked plate apertures; the 3D array include discrete supports arranged in columns in one or more wells; the supports include tube inner walls or be suspended in tubes, the tubes being secured in stacked, two-dimensional (2D) frameworks; or the supports include beads contained in porous enclosures having non-porous side walls and being secured in stacked, 2D frameworks. A support transfer device enables transfer of solid-phase supports used in a 3D array. Such apparatus includes: a rack of rods sized to be inserted through supports and a mechanism to prevent supports from coming off the rack; tubes connected to a vacuum manifold to suction supports one Z plane at a time; or a transfer block having recesses to receive one or more support and at least one gate withholding supports from passing through the gate when in a closed position. A method of 3D synthesis includes: a) functionalizing solid-phase supports; b) placing supports in a 3D array; and c) performing parallel synthesis with 3D diversity. At least one unique R1 group member may be assigned to each Z plane.
摘要翻译：三维（3D）固相支架阵列适用于提供具有3D多样性的分子库的并行合成。 3D阵列中的各个位置可以分配给文库中的选定分子，使得分子可以在这些位置合成并从这些位置检索。此外，支撑件包括堆叠板中的孔壁; 支撑件可以悬挂在堆叠的板孔内; 3D阵列包括以一个或多个孔中的列布置的离散支撑件; 支撑件包括管内壁或悬挂在管中，管被固定在堆叠的二维（2D）框架中; 或者支撑体包括包含在具有无孔侧壁并且被固定在堆叠的2D框架中的多孔封壳中的珠子。支撑转移装置能够传送在3D阵列中使用的固相支撑。这种装置包括：尺寸适于插入通过支撑件的杆状杆状物和防止支架从架子上脱落的机构; 连接到真空歧管以抽吸的管一次支撑一个Z平面; 或具有用于接收一个或多个支撑件的凹部的传递块和当处于关闭位置时至少一个闸门预扣支撑件不经过闸门。一种3D合成方法包括：a）将固相载体官能化; b）将支撑放置在3D阵列中; 和c）使用3D分集进行并行合成。可以将至少一个唯一的R1组成员分配给每个Z平面。

10. 发明申请

US20030203390A1 Coded particles for process sequence tracking in combinatorial compound library preparation 审中-公开
标题翻译：用于组合化合物库制备过程序列跟踪的编码粒子
公开(公告)号：US20030203390A1
公开(公告)日：2003-10-30
申请号：US10428848
申请日：2003-05-05
发明人： Paul H. Kaye , Mark C. Tracey
IPC分类号： C12Q001/68 , C12M001/34 , G01N033/543
CPC分类号： C07K1/045 , B01J19/0046 , B01J2219/005 , B01J2219/00502 , B01J2219/00547 , B01J2219/00549 , B01J2219/00556 , B01J2219/00558 , B01J2219/0056 , B01J2219/00585 , B01J2219/00592 , B01J2219/00596 , C07K1/047 , C40B70/00 , G06K19/06009
摘要： A solid support particle adapted for use in Combinatorial Chemistry Techniques characterised in that the particle is marked with machine readable code.
摘要翻译：适用于组合化学技术的固体支持颗粒，其特征在于该颗粒用机器可读代码标记。

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