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1. 发明申请

US20180201998A1 COMPOSITIONS AND METHODS FOR DETECTION OF GENETIC DEAFNESS GENE MUTATION 审中-公开
公开(公告)号：US20180201998A1
公开(公告)日：2018-07-19
申请号：US15744719
申请日：2015-07-14
申请人： CapitalBio Corporation , Tsinghua University
发明人： Guangxin XIANG , Xuezhong LIU , Xingping CHAI , Wanli XING , Jing CHENG
IPC分类号： C12Q1/6883
CPC分类号： C12Q1/6883 , C12Q1/6827 , C12Q2600/156 , C12Q2600/16 , C12Q2563/103 , C12Q2563/149 , C12Q2565/519
摘要： In one aspect, a kit for detection of genetic gene mutation is provided, which is used for detecting nine deafness gene mutations of Caucasian populations, including GJB2 (c.35delG, c.167delT, c.132G>C, and c.269T>C), GJB6 (c.del309kb), SLC26A4 (c.707T>C and c.1246A>C), 12S rRNA (m.1555A>G and m.7444G>A). In another aspect, a method is provided, which method comprises labeling a target molecule with a luminophore, coupling the target molecule to a particle, and binding to a probe molecule on microarray. In some aspects, this technology, with high sensitivity, enables the detection and interpretation of molecular interactions in an efficient way.

2. 发明申请

US20180080020A1 MICROFLUIDIC DEVICES AND METHODS OF USE IN THE FORMATION AND CONTROL OF NANOREACTORS 审中-公开
公开(公告)号：US20180080020A1
公开(公告)日：2018-03-22
申请号：US15694108
申请日：2017-09-01
申请人： Raindance Technologies, Inc.
发明人： Darren Roy Link , Laurent Boitard , Jeffrey Branciforte , Yves Charles , Gilbert Feke , John Q. Lu , David Marran , Ahmadali Tabatabai , Michael Weiner , Wolfgang Hinz , Jonathan M. Rothberg
IPC分类号： C12N15/10 , G01N33/68 , B01F13/00 , G01N33/543 , G01N33/536 , C40B70/00 , C40B60/12 , C40B60/08 , C40B50/08 , C40B40/04 , C40B30/04 , C12Q1/68 , C07K1/04 , B82Y30/00 , B82Y5/00 , B01L3/00 , B01J19/00 , G01N33/542 , G01N21/64 , B01L7/00 , B01F3/08
CPC分类号： B01F3/0807 , B01F13/0071 , B01F13/0076 , B01J19/0046 , B01J2219/00286 , B01J2219/00459 , B01J2219/00466 , B01J2219/00468 , B01J2219/00479 , B01J2219/005 , B01J2219/00576 , B01J2219/00599 , B01J2219/00702 , B01J2219/0072 , B01J2219/00743 , B01L3/5027 , B01L3/502715 , B01L3/50273 , B01L3/502746 , B01L3/502761 , B01L3/502784 , B01L3/502792 , B01L3/565 , B01L7/52 , B01L2200/027 , B01L2200/0621 , B01L2200/0647 , B01L2200/0652 , B01L2200/0673 , B01L2300/0816 , B01L2300/0864 , B01L2300/0867 , B01L2300/0896 , B01L2400/0415 , B01L2400/0424 , B01L2400/0487 , B01L2400/084 , B82Y5/00 , B82Y30/00 , C07K1/047 , C12N15/1075 , C12Q1/6804 , C12Q1/6816 , C12Q1/6825 , C12Q1/6834 , C12Q1/686 , C12Q1/6869 , C12Q1/6874 , C12Q1/6876 , C12Q2525/161 , C12Q2561/119 , C12Q2563/103 , C12Q2563/107 , C12Q2563/159 , C12Q2565/537 , C12Q2565/629 , C12Q2600/16 , C40B30/04 , C40B40/04 , C40B50/08 , C40B60/08 , C40B60/12 , C40B70/00 , G01N21/6428 , G01N21/6445 , G01N21/6458 , G01N33/536 , G01N33/542 , G01N33/543 , G01N33/6845 , G01N33/6854 , G01N2500/10
摘要： The present invention provides novel microfluidic devices and methods that are useful for performing high-throughput screening assays and combinatorial chemistry. The invention provides for aqueous based emulsions containing uniquely labeled cells, enzymes, nucleic acids, etc., wherein the emulsions further comprise primers, labels, probes, and other reactants. An oil based carrier-fluid envelopes the emulsion library on a microfluidic device, such that a continuous channel provides for flow of the immiscible fluids, to accomplish pooling, coalescing, mixing, sorting, detection, etc., of the emulsion library.

3. 发明申请

US20170362651A1 LABELED NUCLEOTIDE COMPOSITIONS AND METHODS FOR NUCLEIC ACID SEQUENCING 审中-公开
公开(公告)号：US20170362651A1
公开(公告)日：2017-12-21
申请号：US15600979
申请日：2017-05-22
申请人： Quantum-Si Incorporated
发明人： Jonathan M. Rothberg , Jeremy Lackey , Brian Reed , Xinghua Shi , Haidong Huang , David Dodd
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6869 , C07H21/00 , C12Q1/6816 , C12Q2525/101 , C12Q2525/197 , C12Q2525/301 , C12Q2563/103
摘要： Methods of sequencing molecules based on luminescence lifetimes and/or intensities are provided. In some aspects, methods of sequencing nucleic acids involve determining the luminescence lifetimes, and optionally luminescence intensities, of a series of luminescently labeled nucleotides incorporated during a nucleic acid sequencing reaction. In some aspects, the disclosure provides compositions comprising luminescently labeled nucleotides.

4. 发明申请

US20170356030A1 COMPOSITIONS, SYSTEMS, AND METHODS FOR DETECTING THE PRESENCE OF POLYMER SUBUNITS USING CHEMILUMINESCENCE 审中-公开
公开(公告)号：US20170356030A1
公开(公告)日：2017-12-14
申请号：US15507465
申请日：2015-09-10
申请人： ILLUMINA, INC.
发明人： Boyan Boyanov , Liangliang Qiang , Kevin L. Gunderson , Kay Klausing , Lea Pickering , Cyril De Lattre , Tarun Khurana
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6818 , C12Q1/6816 , C12Q1/6874 , C12Q2533/101 , C12Q2535/113 , C12Q2535/122 , C12Q2563/103 , C12Q2563/125
摘要： Under one aspect, a composition includes a substrate; a first polynucleotide coupled to the substrate; a second polynucleotide hybridized to the first polynucleotide; and a catalyst coupled to a first nucleotide of the second polynucleotide, the catalyst being operable to cause a chemiluminogenic molecule to emit a photon. Under another aspect, a method includes providing a catalyst operable to cause a first chemiluminogenic molecule to emit a photon; providing a substrate; providing a first polynucleotide coupled to the substrate; hybridizing a second polynucleotide to the first polynucleotide; coupling a first quencher to a first nucleotide of the second polynucleotide; and inhibiting, by the first quencher, photon emission by the first chemiluminogenic molecule.

5. 发明申请

US20170283858A1 Simple LAMP PCR Design For Low Resource Setting And Minimal Environmental Footprint 审中-公开
公开(公告)号：US20170283858A1
公开(公告)日：2017-10-05
申请号：US15476753
申请日：2017-03-31
申请人： BOARD OF TRUSTEES OF THE UNIVERSITY OF ARKANSAS , NowDiagnostics
发明人： Robert Beitle , Christa N. Hestekin , Ahmed Elmasheiti , Kimberly Cribbs , Michael Rienisch , Bryce Cameron Jones , Allysa Swearingen , Brandon Hart , Kevin Clark , Vicki Thompson
IPC分类号： C12Q1/68 , B01D21/26 , B01L7/00 , C12N15/10 , G01N1/30
CPC分类号： B01D21/262 , B01L7/00 , B01L9/06 , B01L2200/10 , B01L2200/147 , B01L2300/0627 , B01L2300/18 , B01L2300/1877 , C12N15/1006 , C12Q1/6806 , G01N1/30 , G01N31/22 , G01N2001/302 , C12Q2523/32 , C12Q2527/101 , C12Q2531/119 , C12Q2547/107 , C12Q2563/103 , C12Q2563/107 , C12Q2527/125
摘要： The present invention provides a device and method for testing a material for the presence of DNA. The system includes a centrifuge, a microchip performing cell lysis and an enclosure that contains an isothermal ballast material and chromogenic agent that melts at a specific temperature and displays a color change, respectively.

6. 发明授权

US09540689B2 Method of determining the nucleotide sequence of oligonucleotides and DNA molecules 有权
标题翻译：确定寡核苷酸和DNA分子核苷酸序列的方法
公开(公告)号：US09540689B2
公开(公告)日：2017-01-10
申请号：US14941403
申请日：2015-11-13
申请人： LIFE TECHNOLOGIES CORPORATION
发明人： Peter Williams
IPC分类号： C12Q1/68 , C12P19/34
CPC分类号： C12Q1/6869 , C12Q1/6816 , C12Q1/6825 , C12Q2533/101 , C12Q2563/103 , C12Q2563/107 , C12Q2563/116 , C12Q2565/301 , C12Q2565/629 , G01N33/573 , G01N33/6863
摘要： The present invention relates to a novel method for analyzing nucleic acid sequences based on real-time detection of DNA polymerase-catalyzed incorporation of each of the four nucleotide bases, supplied individually and serially in a microfluidic system, to a reaction cell containing a template system comprising a DNA fragment of unknown sequence and an oligonucleotide primer. Incorporation of a nucleotide base into the template system can be detected by any of a variety of methods including but not limited to fluorescence and chemiluminescence detection. Alternatively, microcalorimetic detection of the heat generated by the incorporation of a nucleotide into the extending template system using thermopile, thermistor and refractive index measurements can be used to detect extension reactions.
摘要翻译：本发明涉及一种用于分析核酸序列的新方法，该方法基于DNA聚合酶催化的四个核苷酸碱基中的每一个（在微流体系统中单独提供和连续供给）中并入到包含模板系统的反应池中包含未知序列的DNA片段和寡核苷酸引物。将核苷酸碱基掺入模板系统可以通过多种方法中的任何一种检测，包括但不限于荧光和化学发光检测。或者，可以使用通过使用热电堆，热敏电阻和折射率测量将核苷酸并入扩展模板系统产生的热的微量热法测定来检测延伸反应。

7. 发明授权

US09534216B2 Microfluidic devices and methods of use in the formation and control of nanoreactors 有权
标题翻译：微流体装置和用于形成和控制纳米反应器的方法
公开(公告)号：US09534216B2
公开(公告)日：2017-01-03
申请号：US14248991
申请日：2014-04-09
申请人： Raindance Technologies, Inc.
发明人： Darren R. Link , Laurent Boitard , Jeffrey Branciforte , Yves Charles , Gilbert Feke , John Q. Lu , David Marran , Ahmadali Tabatabai , Michael Weiner , Wolfgang Hinz , Jonathan M. Rothberg
IPC分类号： G01N15/10 , C12Q1/68 , G01N21/00 , G01N21/21 , C12N15/10 , B01F13/00 , B01J19/00 , B82Y5/00 , B82Y30/00 , C07K1/04 , C40B30/04 , C40B40/04 , C40B50/08 , C40B60/08 , C40B60/12 , C40B70/00 , G01N33/536 , G01N33/68 , G01N33/543 , G01N33/50 , G01N33/00 , B01L3/00 , B01F3/08 , G01N33/542 , G01N21/64 , B01L7/00
CPC分类号： C12N15/1075 , B01F3/0807 , B01F13/0071 , B01F13/0076 , B01J19/0046 , B01J2219/00286 , B01J2219/00459 , B01J2219/00466 , B01J2219/00468 , B01J2219/00479 , B01J2219/005 , B01J2219/00576 , B01J2219/00599 , B01J2219/00702 , B01J2219/0072 , B01J2219/00743 , B01L3/5027 , B01L3/502715 , B01L3/50273 , B01L3/502746 , B01L3/502761 , B01L3/502784 , B01L3/502792 , B01L3/565 , B01L7/52 , B01L2200/027 , B01L2200/0621 , B01L2200/0647 , B01L2200/0652 , B01L2200/0673 , B01L2300/0816 , B01L2300/0864 , B01L2300/0867 , B01L2300/0896 , B01L2400/0415 , B01L2400/0424 , B01L2400/0487 , B01L2400/084 , B82Y5/00 , B82Y30/00 , C07K1/047 , C12Q1/6804 , C12Q1/6816 , C12Q1/6825 , C12Q1/6834 , C12Q1/686 , C12Q1/6869 , C12Q1/6874 , C12Q1/6876 , C12Q2525/161 , C12Q2561/119 , C12Q2563/103 , C12Q2563/107 , C12Q2563/159 , C12Q2565/537 , C12Q2565/629 , C12Q2600/16 , C40B30/04 , C40B40/04 , C40B50/08 , C40B60/08 , C40B60/12 , C40B70/00 , G01N21/6428 , G01N21/6445 , G01N21/6458 , G01N33/536 , G01N33/542 , G01N33/543 , G01N33/6845 , G01N33/6854 , G01N2500/10
摘要： The present invention provides novel microfluidic devices and methods that are useful for performing high-throughput screening assays and combinatorial chemistry. The invention provides for aqueous based emulsions containing uniquely labeled cells, enzymes, nucleic acids, etc., wherein the emulsions further comprise primers, labels, probes, and other reactants. An oil based carrier-fluid envelopes the emulsion library on a microfluidic device, such that a continuous channel provides for flow of the immiscible fluids, to accomplish pooling, coalescing, mixing, sorting, detection, etc., of the emulsion library.
摘要翻译：本发明提供了可用于进行高通量筛选测定和组合化学的新型微流体装置和方法。本发明提供了含有唯一标记的细胞，酶，核酸等的含水基乳液，其中乳液还包含引物，标记，探针和其它反应物。油基载体流体在微流体装置上包封乳剂库，使得连续通道提供不混溶流体的流动，以实现乳液库的汇集，聚结，混合，分选，检测等。

8. 发明申请

US20150045251A1 DUPLEX CHROMOGENIC ASSAY FOR IN SITU DETECTION OF NUCLEIC ACIDS 审中-公开
标题翻译：用于检测核酸的双重色谱测定
公开(公告)号：US20150045251A1
公开(公告)日：2015-02-12
申请号：US14381942
申请日：2013-03-06
申请人： Advanced Cellular Diagnostics, Inc.
发明人： Li-Chong Wang , Nan Su , Xiao-Jun Ma , Yuling Luo
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6841 , C12Q2600/16 , C12Q2537/143 , C12Q2563/103 , C12Q2563/125
摘要： The disclosure provides methods of detecting two or more target nucleic acids. The methods can include the steps of contacting a sample with two or more label probes having distinct enzyme labels and targeting distinct nucleic acid targets, binding the two or more label probes to the target nucleic acids by hybridization; contacting the sample with a first substrate for the first enzyme of the first label probe; reacting the first substrate with the first enzyme, thereby producing a first detectable signal; contacting the sample with a second substrate for the second enzyme of the second label probe; reacting the second substrate with the second enzyme, thereby producing a second detectable signal; and detecting the first detectable signal and the second detectable signal, thereby detecting the first and second target nucleic acids in the sample.
摘要翻译：本公开提供了检测两种或更多种靶核酸的方法。所述方法可以包括以下步骤：将样品与具有不同酶标记的两个或更多个标记探针接触并靶向不同的核酸靶标，通过杂交将两个或多个标记探针结合到靶核酸; 使样品与第一标记探针的第一酶的第一底物接触; 使第一底物与第一酶反应，从而产生第一可检测信号; 使所述样品与所述第二标记探针的第二酶的第二底物接触; 使第二底物与第二酶反应，从而产生第二可检测信号; 并检测第一可检测信号和第二可检测信号，从而检测样品中的第一和第二靶核酸。

9. 发明授权

US08865077B2 Apparatus for single-molecule detection 有权
标题翻译：单分子检测装置
公开(公告)号：US08865077B2
公开(公告)日：2014-10-21
申请号：US12801503
申请日：2010-06-11
申请人： Chung-Fan Chiou , Rung-Ywan Tsai , Yu-Tang Li , Chih-Tsung Shih , Ming-Chia Li , Chang-Sheng Chu , Shuang-Chao Chung , Jung-Po Chen , Ying-Chih Pu
发明人： Chung-Fan Chiou , Rung-Ywan Tsai , Yu-Tang Li , Chih-Tsung Shih , Ming-Chia Li , Chang-Sheng Chu , Shuang-Chao Chung , Jung-Po Chen , Ying-Chih Pu
IPC分类号： G01N33/487 , G01N33/50 , G01N33/52 , G01N21/01 , G01N21/64 , C40B60/12 , G01J1/04 , G01J1/58 , G02B6/34 , C12Q1/68
CPC分类号： G01J1/58 , C12Q1/68 , C12Q1/6869 , C12Q1/6874 , G01J1/0425 , G02B6/34 , Y10S977/954 , Y10S977/957 , Y10S977/958 , C12Q2563/103 , C12Q2565/607
摘要： An apparatus for detecting an object capable of emitting light. The apparatus comprises a light source and a waveguide. The waveguide comprises a core layer and a first cladding layer. At least one nanowell is formed in at least the first cladding layer. The apparatus further comprises a light detector. The light detector can detect a light emitted from a single molecule object contained in the at least one nanowell.
摘要翻译：一种用于检测能够发光的物体的装置。该装置包括光源和波导。波导包括芯层和第一覆层。在至少第一包层中形成至少一个纳米线。该装置还包括光检测器。光检测器可以检测从包含在至少一个纳米管中的单个分子对象发射的光。

10. 发明申请

US20130295560A1 METHOD OF DETERMINING THE NUCLEOTIDE SEQUENCE OF OLIGONUCLEOTIDES AND DNA MOLECULES 有权
公开(公告)号：US20130295560A1
公开(公告)日：2013-11-07
申请号：US13913433
申请日：2013-06-08
申请人： Arizona Board of Regents, a body corporate of the State of AZ, acting for and on behalf of AZ Sta
发明人： Peter Williams , Thomas J. Taylor , Daniel J.B. Williams , Ian Gould , Mark A. Hayes
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6869 , C12Q1/6816 , C12Q1/6825 , C12Q2533/101 , C12Q2563/103 , C12Q2563/107 , C12Q2563/116 , C12Q2565/301 , C12Q2565/629 , G01N33/573 , G01N33/6863
摘要： The present invention relates to a novel method for analyzing nucleic acid sequences based on real-time detection of DNA polymerase-catalyzed incorporation of each of the four nucleotide bases, supplied individually and serially in a microfluidic system, to a reaction cell containing a template system comprising a DNA fragment of unknown sequence and an oligonucleotide primer. Incorporation of a nucleotide base into the template system can be detected by any of a variety of methods including but not limited to fluorescence and chemiluminescence detection. Alternatively, microcalorimetic detection of the heat generated by the incorporation of a nucleotide into the extending template system using thermopile, thermistor and refractive index measurements can be used to detect extension reactions.

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