专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明授权

US4935352A Expression vector for animal cell line and use thereof 失效
标题翻译：动物细胞系的表达载体及其用途
公开(公告)号：US4935352A
公开(公告)日：1990-06-19
申请号：US919798
申请日：1986-10-16
申请人： Koichi Igarashi , Reiko Sasada , Tomoko Fujii , Ryuji Marumoto
发明人： Koichi Igarashi , Reiko Sasada , Tomoko Fujii , Ryuji Marumoto
IPC分类号： C07K14/485 , C07K14/55 , C07K16/00 , C12N15/85
CPC分类号： C12N15/85 , C07K14/485 , C07K14/55 , C07K16/00 , C07K2319/02
摘要： An animal cell line transformed with an expression vector for an animal cell, the expression vector which contains a DNA segment comprising:(a) a DNA sequence coding for a signal peptide of an animal cell-derived protein,(b) a second DNA sequence coding for a different protein from the signal protein joined downstream of said signal peptide encoding DNA sequence without causing any reading frame shift, and(c) a promoter DNA sequence capable of functioning in an animal cell, wherein the promoter sequence is positioned upstream of the signal peptide encoding DNA sequence, can produce glycosylated proteins advantageously as secretable proteins.
摘要翻译：用动物细胞表达载体转化的动物细胞系，含有DNA片段的表达载体包含：（a）编码动物细胞来源的蛋白质的信号肽的DNA序列，（b）第二DNA序列编码来自所述信号肽编码DNA序列下游的信号蛋白的不同蛋白质，而不引起任何阅读框移动，和（c）能够在动物细胞中起作用的启动子DNA序列，其中启动子序列位于信号肽编码DNA序列，可以有利地产生糖基化的蛋白质作为可分泌的蛋白质。

2. 发明授权

US06825165B1 Betacellulin modification 失效
标题翻译：白细胞素修饰
公开(公告)号：US06825165B1
公开(公告)日：2004-11-30
申请号：US09857815
申请日：2001-06-08
申请人： Takashi Ito , Mitsuyo Kondo , Yoko Tanaka , Masayuki Kobayashi , Koichi Igarashi , Reiko Sasada , Osamu Nishimura
发明人： Takashi Ito , Mitsuyo Kondo , Yoko Tanaka , Masayuki Kobayashi , Koichi Igarashi , Reiko Sasada , Osamu Nishimura
IPC分类号： C07K1400
CPC分类号： C07K14/475 , A61K38/00 , C07K14/485
摘要： The betacellulin mutein or its salt of the present invention is useful for better therapeutic drug for diabetes. Since they have intact BTC activity and reduced EGF activity, and no antigenicity-related problems.
摘要翻译：本发明的细胞素突变蛋白或其盐可用于更好的治疗药物用于糖尿病。因为它们具有完整的BTC活性和降低的EGF活性，并且没有抗原性相关的问题。

3. 发明授权

US5360896A Glycosylated FGF 失效
标题翻译：糖基化FGF
公开(公告)号：US5360896A
公开(公告)日：1994-11-01
申请号：US7089
申请日：1993-01-19
申请人： Masaharu Senoo , Reiko Sasada , Koichi Igarashi
发明人： Masaharu Senoo , Reiko Sasada , Koichi Igarashi
IPC分类号： G06F1/28 , A61K38/00 , C07K14/50 , C07K13/00
CPC分类号： C07K14/50 , A61K38/00 , C07K2319/036 , C07K2319/75
摘要： Disclosed are (1) a mutein of a fibroblast growth factor (FGF), the DNA having introduced therein at least one nucleotide sequence coding for a glycosylation site, (2) a DNA coding for the mutein of (1), (3) a vector containing the DNA of (2), (4) a transformant transformed with the vector of (3), and (5) a process for producing the mutein which comprises cultivating in a culture medium the transformant of a yeast or animal cell transformed with a vector of (3), and producing and accumulating the mutein of (1) in the culture medium, whereby the FGF mutein into which at least one glycosylation site has been introduced is improved in productivity, stability and activities, and advantageously used as medicine.
摘要翻译：公开了（1）成纤维细胞生长因子（FGF）的突变蛋白，其中引入了至少一个编码糖基化位点的核苷酸序列的DNA，（2）编码（1），（3）的突变蛋白的DNA 含有（2）的DNA的载体，（4）用（3）的载体转化的转化体，和（5）一种产生突变蛋白的方法，其包括在培养基中培养转化了酵母或动物细胞的转化体（3）的载体，并且在培养基中产生和积累（1）的突变蛋白，从而提高了其中引入至少一个糖基化位点的FGF突变蛋白的生产力，稳定性和活性，并且有利地用作药物。

4. 发明授权

US5852177A Basic fibroblast growth factor (bFGF) muteins 失效
标题翻译：碱性成纤维细胞生长因子（bFGF）突变蛋白
公开(公告)号：US5852177A
公开(公告)日：1998-12-22
申请号：US301460
申请日：1989-01-24
申请人： Masaharu Senoo , Reiko Sasada , Tsutomu Kurokawa , Koichi Igarashi
发明人： Masaharu Senoo , Reiko Sasada , Tsutomu Kurokawa , Koichi Igarashi
IPC分类号： A61K38/00 , C07K14/50 , C07K14/55 , C07K16/22
CPC分类号： C07K16/22 , C07K14/503 , C07K14/55 , A61K38/00 , C07K2319/00 , C07K2319/02 , C07K2319/75
摘要： A mutein of basic fibroblast growth factors (bFGF) possesses fibroblast growth promoting activity, growth stimulating activity of capillary endothelial cells and angiogenic activity, has high stability and is low toxicity. And therefore, the present mutein is advantageously used as a healing accelerator for burns etc., a therapeutic drug for thrombosis etc., and a cell cultivation promoter.
摘要翻译：碱性成纤维细胞生长因子（bFGF）的突变蛋白具有成纤维细胞生长促进活性，毛细血管内皮细胞生长刺激活性和血管生成活性，稳定性高，毒性低。因此，本发明的突变蛋白有利地用作燃烧等的愈合促进剂，血栓形成治疗药等，以及细胞培养促进剂。

5. 发明授权

US5464943A DNA encoding glycosylated FGF and production thereof 失效
标题翻译：编码糖基化FGF的DNA及其生产
公开(公告)号：US5464943A
公开(公告)日：1995-11-07
申请号：US275635
申请日：1994-07-15
申请人： Masaharu Senoo , Reiko Sasada , Koichi Igarashi
发明人： Masaharu Senoo , Reiko Sasada , Koichi Igarashi
IPC分类号： A61K38/00 , C07K14/50 , C12N15/62 , C07H21/00 , C12N15/18 , C12N15/63
CPC分类号： C07K14/503 , C07K14/50 , C12N15/625 , A61K38/00 , C07K2319/036 , C07K2319/75 , C07K2319/91
摘要： Disclosed are (1) a mutein of a fibroblast growth factor (FGF), the DNA having introduced therein at least one nucleotide sequence coding for a glycosylation site, (2) a DNA coding for the mutein of (1), (3) a vector containing the DNA of (2), (4) a transformant transformed with the vector of (3), and (5) a process for producing the mutein which comprises cultivating in a culture medium the transformant of a yeast or animal cell transformed with a vector of (3), and producing and accumulating the mutein of (1) in the culture medium, whereby the FGF mutein into which at least one glycosylation site has been introduced is improved in productivity, stability and activities, and advantageously used as medicine.
摘要翻译：公开了（1）成纤维细胞生长因子（FGF）的突变蛋白，其中引入了至少一个编码糖基化位点的核苷酸序列的DNA，（2）编码（1），（3）的突变蛋白的DNA 含有（2）的DNA的载体，（4）用（3）的载体转化的转化体，和（5）一种产生突变蛋白的方法，其包括在培养基中培养转化了酵母或动物细胞的转化体（3）的载体，并且在培养基中产生和积累（1）的突变蛋白，从而提高了其中引入至少一个糖基化位点的FGF突变蛋白的生产力，稳定性和活性，并且有利地用作药物。

6. 发明授权

US07556926B2 Methods for screening insulin-sensitizing agents 失效
标题翻译：筛选胰岛素敏化剂的方法
公开(公告)号：US07556926B2
公开(公告)日：2009-07-07
申请号：US10515720
申请日：2003-05-22
申请人： Hideaki Tojo , Tsutomu Henta , Reiko Sasada
发明人： Hideaki Tojo , Tsutomu Henta , Reiko Sasada
IPC分类号： G01N33/55 , G01N33/554 , A61K38/24
CPC分类号： C07K14/47 , A61K31/7088
摘要： A compound or its salt inhibiting the activity of a protein having an amino acid sequence which is the same or substantially the same amino acid sequence as the amino acid sequence represented by SEQ ID NO: 1, an extracellular domain of the protein above, an antibody, an antisense polynucleotide, etc. are usable as insulin-sensitizing agents and preventives and/or remedies for diabetes, obesity, hyperlipemia, arteriosclerosis, hypertension, heart disease, and so on.
摘要翻译：抑制具有与SEQ ID NO：1表示的氨基酸序列相同或基本相同的氨基酸序列的蛋白质的活性的化合物或其盐，上述蛋白质的细胞外结构域，抗体，反义多核苷酸等可用作糖尿病，肥胖症，高脂血症，动脉硬化，高血压，心脏病等的胰岛素敏化剂和预防剂和/或治疗药物。

7. 发明授权

US06709837B1 Polypeptide and production thereof 失效
标题翻译：多肽及其生产
公开(公告)号：US06709837B1
公开(公告)日：2004-03-23
申请号：US07488696
申请日：1990-03-05
申请人： Kazuo Nakahama , Yoshihiko Kaisho , Koji Yoshimura , Reiko Sasada
发明人： Kazuo Nakahama , Yoshihiko Kaisho , Koji Yoshimura , Reiko Sasada
IPC分类号： C12N500
CPC分类号： C07K16/22 , A61K38/00 , C07K14/475 , C07K14/48
摘要： Disclosed are (1) a polypeptide (I) including the following amino acid sequence (II) in a molecule thereof: TyrAlaGluHisLysSerHisArgGlyGluTyrSerValCys (II) AspSerGluSerLeuTrpValThrAspLysSerSerAlaIle spIleArgGlyHisGlnValThrValLeuGlyGluIleLys ThrGlyAsnSerProValLysGlnTyrPheTyrGluThrArg CysLysGluAlaArgProValLysAsnGlyCysArgGlyIle AspAspLysHisTrpAsnSerGlnCysLysThrSerGlnThr TyrValArgAlaLeuThrSerGluAsnAsnLysLeuValGly TrpArgTrpIleArgIleAspThrSerCysValCysAlaLeu SerArgLysIleGlyArg (2) a DNA sequence coding for the polypeptide described in (1), (3) a vector including the DNA described in (2), (4) a transformant transformed by the vector described in (3), and (5) a process for producing the polypeptide (I) which comprises cultivating the transformant described in (4) in a culture medium to produce and accumulate the polypeptide described in (1) in a culture. The polypeptide is useful as a reagent for studies relating to the differentiation, growth and survival of animal cells, and may also be useful as a drug.
摘要翻译：公开了（1）在其分子中包含以下氨基酸序列（II）的多肽（I）：（2）编码（1），（3）中描述的多肽的DNA序列，包含在（2）中，（4）由（3）中描述的载体转化的转化体，和（5）生产多肽（I）的方法，其包括在培养基中培养（4）中描述的转化体以产生和积累（1）中所述的多肽在培养物中。该多肽可用作用于与动物细胞的分化，生长和存活相关的研究的试剂，并且也可用作药物。

8. 发明授权

US6015552A Use of nerve growth factor-2 (NGF-2) /neurotrophin-3 (NT-3) to promote leukocyte proliferation 失效
标题翻译：神经生长因子-2（NGF-2）/神经营养因子-3（NT-3）的使用促进白细胞增殖
公开(公告)号：US6015552A
公开(公告)日：2000-01-18
申请号：US910691
申请日：1997-08-13
申请人： Tatsuya Watanabe , Sumie Yoshitomi , Reiko Sasada
发明人： Tatsuya Watanabe , Sumie Yoshitomi , Reiko Sasada
IPC分类号： A61K38/00 , C07K14/475 , A61K38/19 , A61K38/18
CPC分类号： C07K14/475 , A61K38/00
摘要： Human Nerve Growth Factor-2 (NGF-2, also known as Neurotrophin-3 or NT-3) has been found to promote the proliferation of peripheral blood leukocytes. The invention provides methods using hNGF-2 polypeptides in the treatment of neutropenia.
摘要翻译：已经发现人神经生长因子-2（NGF-2，也称为神经营养因子-3或NT-3）促进外周血白细胞的增殖。本发明提供了使用hNGF-2多肽治疗嗜中性白细胞减少症的方法。

9. 发明申请

US20060014679A1 Insulin resistance improving agents 失效
标题翻译：胰岛素抵抗改善剂
公开(公告)号：US20060014679A1
公开(公告)日：2006-01-19
申请号：US10515720
申请日：2003-05-22
申请人： Hideaki Tojo , Tsutomu Henta , Reiko Sasada
发明人： Hideaki Tojo , Tsutomu Henta , Reiko Sasada
IPC分类号： A61K48/00 , A61K38/22 , C07H21/02 , C07K14/575
CPC分类号： C07K14/47 , A61K31/7088
摘要： A compound or its salt inhibiting the activity of a protein having an amino acid sequence which is the same or substantially the same amino acid sequence as the amino acid sequence represented by SEQ ID NO: 1, an extracellular domain of the protein above, an antibody, an antisense polynucleotide, etc. are usable as insulin-sensitizing agents and preventives and/or remedies for diabetes, obesity, hyperlipemia, arteriosclerosis, hypertension, heart disease, and so on.
摘要翻译：抑制具有与SEQ ID NO：1表示的氨基酸序列相同或基本相同的氨基酸序列的蛋白质的活性的化合物或其盐，上述蛋白质的细胞外结构域，抗体，反义多核苷酸等可用作糖尿病，肥胖症，高脂血症，动脉硬化，高血压，心脏病等的胰岛素敏化剂和预防剂和/或治疗药物。

10. 发明授权

US06183971B2 Human betacellulin-specific antibodies and uses thereof 失效
标题翻译：人类细胞素特异性抗体及其用途
公开(公告)号：US06183971B2
公开(公告)日：2001-02-06
申请号：US08663191
申请日：1996-06-11
申请人： Reiko Sasada , Tatsuya Watanabe , Yukio Toyoda
发明人： Reiko Sasada , Tatsuya Watanabe , Yukio Toyoda
IPC分类号： G01N3353
CPC分类号： C07K14/475 , A61K38/00 , C07K16/22 , C07K2317/73 , Y10S435/81
摘要： Disclosed are an antibody which have a binding activity to human betacellulin protein or a mutein thereof with specificity; especially a monoclonal antibody which does not have cross reactitivity with human epidermal growth factor (EGF) and human transforming growth factor a (TGF-&agr;), belongs to the immunoglobulin class of IgG, and,specifically binds to human betacellulin protein to neutralize biological activity thereof; a hybridoma for producing the monoclonal antibody; and a method for producing the monoclonal antibody. Said monoclonal antibody neutralizes biological activity of a human BTC protein, and bind to the protein with high sensitivity and specificity, so that they can be used as a therapeutic agent for diseases such as arterial sclerosis and cancers, and also used as a reagent for assaying the human BTC protein or a mutein thereof and as a diagnostic agent for diabetes or complications thereof.
摘要翻译：公开了具有与特异性的人β细胞素蛋白或其突变蛋白结合活性的抗体; 特别是与人表皮生长因子（EGF）和人转化生长因子α（TGF-α）不具有交叉反应性的单克隆抗体属于IgG的免疫球蛋白类，并且特异性结合人β细胞素蛋白以中和生物活性的; 用于产生单克隆抗体的杂交瘤; 和单克隆抗体的制造方法。所述单克隆抗体中和人BTC蛋白的生物活性，并以高灵敏度和特异性结合蛋白质，使其可用作疾病如动脉硬化和癌症的治疗剂，并且还可用作测定试剂人BTC蛋白或其突变蛋白，并且作为糖尿病的诊断剂或其并发症。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式