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    • 3. 发明授权
    • DNA encoding recombinant human lymphotoxin
    • 编码重组人淋巴毒素的DNA
    • US5175268A
    • 1992-12-29
    • US416657
    • 1989-10-03
    • Susumu IwasaTomoko FujiiRyuji MarumotoKoichi Igarashi
    • Susumu IwasaTomoko FujiiRyuji MarumotoKoichi Igarashi
    • C07K14/525C12N15/19
    • C07K14/525
    • Lymphotoxin (LT) mutein (genetically-altered LT) is disclosed that has the following amino acid sequence, or a portion of an active portion of said protein, where 10 to 21 amino acids of LT being deleted from N-terminus and which has Pro or Phe at the N-terminus: ##STR1## Wherein R.sub.1 is Pro or Phe, R.sub.2 is a peptide chain represented by the following sequence:Ala-Gln-Thr-Ala-Arg-Gln-His-Pro-Lys-Met-His-Leu,or a portion thereof and n is 0 or 1.The LT mutein can be recovered in a higher yield and purified more efficiently under mild conditions which does not harm the LT's biological activity, than the whole LT.
    • 公开了具有以下氨基酸序列或所述蛋白质的活性部分的一部分的淋巴毒素(LT)突变蛋白(遗传改变的LT),其中LT的10至21个氨基酸从N末端缺失并且具有Pro 或N末端的Phe:Ala-His-Leu-Ile-Gly-Asp-Pro-Ser-Lys-Gln-Asn-Ser-Leu-Leu-Trp-Arg-Ala-Asn-Thr-Asp -Arg-Ala-Phe-Leu-Gln-Asp-Gly-Phe-Ser-Leu-Ser-Asn-Asn-Ser-Leu-Leu- Val-Pro-Thr-Ser-Gly-Ile-Tyr-Phe-Val - Tyr-Ser-Gln-Val-Val-Phe-Ser-Gly-Lys-Ala-Tyr-Ser-Pro-Lys-Ala-Thr-Ser-Ser-Pro-Leu-Tyr-Leu-Ala-His-Glu -Val-Gln-Leu-Phe-Ser-Ser-Gln-Tyr-Pro-Phe-His-Val-Pro-Leu-Leu-Ser-Ser-Gln-Lys-Met-Val-Tyr-Pro-Gly-Leu -Gln-Glu-Pro-Trp-Leu-His-Ser-Met-Tyr-His-Gly-Ala-Al-Phe-Gln-Leu-Thr-Gln-Gly-Asp-Gln-Leu-Ser-Thr-His -Thr-Asp-Gly-Ile-Pro-His-Leu-Val-Leu-Ser-Pro-Ser-Thr-Val-Phe-Phe-Gly-Ala-Phe-Ala-Leu-OH其中R1是Pro或Phe R2是由以下序列表示的肽链:Ala-Gln-Thr-Ala-Arg-Gln-His-Pro-Lys-Met-His-Leu或端口 并且n为0或1.可以比在整个LT中更高的产率回收LT突变蛋白,并且在不影响LT的生物活性的温和条件下更有效地纯化。