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1. 发明授权

US07105128B2 Dry analytical element using water-soluble indicator for colorimetry 有权
公开(公告)号：US07105128B2
公开(公告)日：2006-09-12
申请号：US10281564
申请日：2002-10-28
申请人： Hideaki Tanaka , Yoshihide Iwaki , Yoshiki Sakaino , Kazuya Kawasaki , Yoshikazu Amano
发明人： Hideaki Tanaka , Yoshihide Iwaki , Yoshiki Sakaino , Kazuya Kawasaki , Yoshikazu Amano
IPC分类号： G01N31/22
CPC分类号： G01N33/525 , G01N33/84
摘要： This invention relates to a dry analytical element using a water-soluble indicator which improves measuring accuracy, which comprises a water-impermeable support, a water-permeable layer and a spreading layer having a function to spread a liquid uniformly, wherein a water-soluble indicator for colorimetry is incorporated into the s preading layer.

2. 发明申请

US20060024770A1 Immunoassay element 失效
公开(公告)号：US20060024770A1
公开(公告)日：2006-02-02
申请号：US11230127
申请日：2005-09-19
申请人： Yoshiki Sakaino , Hitomi Ito , Toshihiro Mori , Osamu Seshimoto , Toshihisa Ito , Yoshikazu Amano
发明人： Yoshiki Sakaino , Hitomi Ito , Toshihiro Mori , Osamu Seshimoto , Toshihisa Ito , Yoshikazu Amano
IPC分类号： G01N33/537 , G01N33/53 , G01N33/543
CPC分类号： G01N33/54386 , G01N2333/924 , Y10S435/966 , Y10S435/969 , Y10S435/97 , Y10S436/807 , Y10S436/81
摘要： The immunoassay element for quantitatively analyzing an antigen by determining the change in enzymatic activity of an enzyme-labelled antigen or antibody caused by an immunological reaction. The immunoassay element comprises a substrate layer containing a non-diffusible substrate which forms a diffusible material in the presence of the labelling enzyme, and a reagent layer containing a fragmenting enzyme for further fragmenting the diffusible material into a lower molecular weight product. As the non-diffusible substrate, a substrate capable of reacting solely with the lebelling enzyme and incapable of reacting the fragmenting enzyme is utilized. When an endo-active glucosidase is used as the labelling enzyme, and an exo-active glucosidase is used the fragmenting enzyme in the reagent layer, the non-diffusible substrate of the substrate layer is preferred to be an endo type selectively reactive substrate, which means a substrate having a reactivity specific to endo-active glucosidase. Highly sensitive assay is realized with high accuracy and high reproducibility and good storage stability.

3. 发明申请

US20050084953A1 Immunoassay element 审中-公开
标题翻译：免疫测定元件
公开(公告)号：US20050084953A1
公开(公告)日：2005-04-21
申请号：US10961459
申请日：2004-10-07
申请人： Yoshiki Sakaino , Hitomi Ito , Toshihiro Mori , Osamu Seshimoto , Toshihisa Ito , Yoshikazu Amano
发明人： Yoshiki Sakaino , Hitomi Ito , Toshihiro Mori , Osamu Seshimoto , Toshihisa Ito , Yoshikazu Amano
IPC分类号： G01N33/543 , G01N33/53
CPC分类号： G01N33/54386 , G01N2333/924 , Y10S435/966 , Y10S435/969 , Y10S435/97 , Y10S436/807 , Y10S436/81
摘要： The immunoassay element for quantitatively analyzing an antigen by determining the change in enzymatic activity of an enzyme-labelled antigen or antibody caused by an immunological reaction. The immunoassay element comprises a substrate layer containing a non-diffusible substrate which forms a diffusible material in the presence of the labelling enzyme, and a reagent layer containing a fragmenting enzyme for further fragmenting the diffusible material into a lower molecular weight product. As the non-diffusible substrate, a substrate capable of reacting solely with the lebelling enzyme and incapable of reacting the fragmenting enzyme is utilized. When an endo-active glucosidase is used as the labelling enzyme, and an exo-active glucosidase is used the fragmenting enzyme in the reagent layer, the non-diffusible substrate of the substrate layer is preferred to be an endo type selectively reactive substrate, which means a substrate having a reactivity specific to endo-active glucosidase. Highly sensitive assay is realized with high accuracy and high reproducibility and good storage stability.
摘要翻译：用于通过确定由免疫反应引起的酶标记的抗原或抗体的酶活性的变化来定量分析抗原的免疫测定元件。免疫测定元件包括含有在标记酶存在下形成可扩散材料的不扩散基底的底物层和含有用于进一步将可扩散材料分解成较低分子量产物的碎裂酶的试剂层。作为非扩散性底物，可以使用能够仅与前体酶反应并且不能使片段化酶反应的底物。当使用内源活性葡糖苷酶作为标记酶，并且使用外源活性葡糖苷酶时，试剂层中的断裂酶，底物层的非扩散性基质优选为内型选择性反应性底物，其中是指具有对内源性葡糖苷酶特异性的反应性的底物。高精度，高重现性和良好的储存稳定性实现了高灵敏度测定。

4. 发明授权

US07820402B2 Immunoassay element 失效
标题翻译：免疫测定元件
公开(公告)号：US07820402B2
公开(公告)日：2010-10-26
申请号：US11230127
申请日：2005-09-19
申请人： Yoshiki Sakaino , Hitomi Ito , Toshihiro Mori , Osamu Seshimoto , Toshihisa Ito , Yoshikazu Amano
发明人： Yoshiki Sakaino , Hitomi Ito , Toshihiro Mori , Osamu Seshimoto , Toshihisa Ito , Yoshikazu Amano
IPC分类号： G01N33/53 , G01N33/543
CPC分类号： G01N33/54386 , G01N2333/924 , Y10S435/966 , Y10S435/969 , Y10S435/97 , Y10S436/807 , Y10S436/81
摘要： The immunoassay element for quantitatively analyzing an antigen by determining the change in enzymatic activity of an enzyme-labelled antigen or antibody caused by an immunological reaction. The immunoassay element comprises a substrate layer containing a non-diffusible substrate which forms a diffusible material in the presence of the labelling enzyme, and a reagent layer containing a fragmenting enzyme for further fragmenting the diffusible material into a lower molecular weight product. As the non-diffusible substrate, a substrate capable of reacting solely with the labelling enzyme and incapable of reacting the fragmenting enzyme is utilized. When an endo-active glucosidase is used as the labelling enzyme, and an exo-active glucosidase is used the fragmenting enzyme in the reagent layer, the non-diffusible substrate of the substrate layer is preferred to be an endo type selectively reactive substrate, which means a substrate having a reactivity specific to endo-active glucosidase. Highly sensitive assay is realized with high accuracy and high reproducibility and good storage stability.
摘要翻译：用于通过确定由免疫反应引起的酶标记的抗原或抗体的酶活性的变化来定量分析抗原的免疫测定元件。免疫测定元件包括含有在标记酶存在下形成可扩散材料的不扩散基底的底物层和含有用于进一步将可扩散材料分解成较低分子量产物的碎裂酶的试剂层。作为非扩散性底物，使用能够仅与标记酶反应并且不能使片段化酶反应的底物。当使用内源活性葡糖苷酶作为标记酶，并且使用外源活性葡糖苷酶时，试剂层中的断裂酶，底物层的非扩散性基质优选为内型选择性反应性底物，其中是指具有对内源性葡糖苷酶特异性的反应性的底物。高精度，高重现性和良好的储存稳定性实现了高灵敏度测定。

5. 发明申请

US20050095626A1 Method for separation and purification method of nucleic acid 审中-公开
标题翻译：核酸分离纯化方法
公开(公告)号：US20050095626A1
公开(公告)日：2005-05-05
申请号：US10932138
申请日：2004-09-02
申请人： Hiroyuki Komazawa , Yoshihide Iwaki , Yoshihiko Makino , Yoshikazu Amano
发明人： Hiroyuki Komazawa , Yoshihide Iwaki , Yoshihiko Makino , Yoshikazu Amano
IPC分类号： G01N33/53 , B01L3/00 , C12M1/00 , C12N15/09 , C12N15/10 , C12Q1/68 , G01N1/34 , G01N37/00
CPC分类号： C12Q1/6806 , B01J2219/00423 , B01J2219/00641 , B01L3/50255 , C12N15/1017 , C12Q1/6837 , G01N1/34 , C12Q2565/501 , C12Q2563/107 , C12Q2531/113 , C12Q2523/308
摘要： The invention provides a rapid, convenient, and automatable method for extracting a highly pure nucleic acid in order to carry out nucleic acid analysis smoothly with high accuracy in an array method. An analyzing method includes analyzing a nucleic acid by an array method, the nucleic acid being separated and purified by a separation and purification method which includes the steps of (a) to (f) identified in the specification.
摘要翻译：本发明提供一种用于提取高纯度核酸的快速，方便和自动化的方法，以便在阵列方法中以高精度平滑地进行核酸分析。分析方法包括通过阵列法分析核酸，所述核酸通过分离和纯化方法分离和纯化，所述分离和纯化方法包括说明书中确定的步骤（a）至（f）。

6. 发明授权

US5066462A Analytical element for measuring enzyme activity 失效
标题翻译：测定酶活性的分析元素
公开(公告)号：US5066462A
公开(公告)日：1991-11-19
申请号：US401494
申请日：1989-08-29
申请人： Kazuya Kawasaki , Mitsutoshi Tanaka , Yoshikazu Amano , Harumi Katsuyama
发明人： Kazuya Kawasaki , Mitsutoshi Tanaka , Yoshikazu Amano , Harumi Katsuyama
IPC分类号： G01N33/50 , C12Q1/00 , C12Q1/26 , C12Q1/48 , C12Q1/52 , G01N33/52
CPC分类号： C12Q1/48 , G01N33/525 , Y10S435/805
摘要： A dry-type analytical element suitable for measuring the activity of alanine aminotransferase in a liquid sample, characterized by incorporating a dye capable of absorbing the electromagnetic waves of 400 to 500 nm into at least one water-permeable layer. The coloring sensitivity of the dry-type analytical element does not increase substantially even under a fluorescent light, and thereby, an accurate measured value can easily be obtained.
摘要翻译：适用于测量液体样品中丙氨酸氨基转移酶活性的干型分析元件，其特征在于将能够吸收400-500nm电磁波的染料掺入到至少一个透水层中。即使在荧光下，干式分析元件的着色灵敏度也不会显着增加，从而可以容易地获得准确的测量值。

7. 发明授权

US5496708A Reagent composition for quantitative analysis of inorganic phosphorous and dry analysis element utilizing the same 失效
标题翻译：用于定量分析无机磷和干分析元素的试剂组合物
公开(公告)号：US5496708A
公开(公告)日：1996-03-05
申请号：US259616
申请日：1994-06-14
申请人： Kazuya Kawasaki , Yoshikazu Amano , Osamu Seshimoto , Kiyoshi Yamada
发明人： Kazuya Kawasaki , Yoshikazu Amano , Osamu Seshimoto , Kiyoshi Yamada
IPC分类号： C12Q1/26 , C12Q1/28 , C12Q1/48 , G01N31/22
CPC分类号： C12Q1/48 , Y10S435/805 , Y10S435/97
摘要： A reagent composition for the quantitative analysis of inorganic phosphorus, composted of xanthosine, purine nucleoside phosphorylase, xanthine oxidase and a color-former. The color-former is a precursor for forming a dye in the presence of hydrogen peroxide and peroxidase. The reagent composition of the invention is improved in storage stability and has a wider determination range. Also provided is a dry analysis element composed of reagent layer containing the inventive reagent composition.
摘要翻译：用于定量分析无机磷的试剂组合物，堆肥的黄嘌呤，嘌呤核苷磷酸化酶，黄嘌呤氧化酶和成色剂。成色剂是在过氧化氢和过氧化物酶存在下形成染料的前体。本发明的试剂组合物的贮存稳定性提高，判定范围更广。还提供了由包含本发明试剂组合物的试剂层组成的干分析元件。

8. 发明授权

US5508173A Analytical element for measurements of enzyme activity 失效
标题翻译：酶活性测定用分析元素
公开(公告)号：US5508173A
公开(公告)日：1996-04-16
申请号：US373885
申请日：1995-01-17
申请人： Yoshikazu Amano , Kazuya Kawasaki , Harumi Katsuyama
发明人： Yoshikazu Amano , Kazuya Kawasaki , Harumi Katsuyama
IPC分类号： G01N31/22 , C12Q1/00 , G01N33/52 , C12Q1/28 , C12Q1/26 , C12Q1/52
CPC分类号： G01N33/525 , G01N33/526
摘要： A multilayer analytical element for measuring enzyme activity in a liquid sample such as a body fluid which is less affected by intrinsic pyruvic acid contained in the liquid sample is disclosed. The analytical element comprises a support, a reagent layer, an optionally provided other layer, and a porous spreading layer. The reagent layer and/or the optionally provided other layer contain pyruvic acid oxidase. The characteristic feature of the analytical element lies in that the spreading layer has a specific void volume in the range of 3-15 .mu.l/cm.sup.2.
摘要翻译：公开了一种用于测量液体样品中的酶活性的多层分析元件，所述液体样品例如体液中较少受到液体样品中所含的固有丙酮酸的影响。分析元件包括载体，试剂层，任选提供的其它层和多孔扩散层。试剂层和/或任选提供的其它层含有丙酮酸氧化酶。分析元件的特征在于扩散层具有在3-15μml/ cm 2范围内的特定空隙体积。

9. 发明授权

US06485927B2 Method for quantitatively determining bicarbonate ion in liquid and dry analysis device 有权
标题翻译：定量测定液体和干燥分析装置中碳酸氢根离子的方法
公开(公告)号：US06485927B2
公开(公告)日：2002-11-26
申请号：US09509610
申请日：2000-03-30
申请人： Hideaki Tanaka , Yoshikazu Amano
发明人： Hideaki Tanaka , Yoshikazu Amano
IPC分类号： C12Q132
CPC分类号： C12Q1/527 , C12Q1/32
摘要： This invention provides a method for the determination of bicarbonate ion existing in liquid using phosphenolpyruvate carboxylase and malate dehydrogenase as conjugate enzyme wherein thioNAD(P)H is used as substrate of malate dehydrogenase. The use of the thioNAD(P)H enables the measurement with visible light, and enables the simple and accurate determination of bicarbonate ion concentration of biological liquid sample in the clinical test.
摘要翻译：本发明提供了使用磷酸烯醇丙酮酸羧化酶和苹果酸脱氢酶作为其中使用thioNAD（P）H作为苹果酸脱氢酶底物的共轭酶的液体中存在的碳酸氢根离子的测定方法。使用thioNAD（P）H可以用可见光进行测量，并能够在临床试验中简单而准确地测定生物液体样品的碳酸氢根离子浓度。

10. 发明授权

US6068989A Method and dry analytical element for determination of bicarbonate ion in liquid 有权
标题翻译：用于测定液体中碳酸氢根离子的方法和干燥分析元素
公开(公告)号：US6068989A
公开(公告)日：2000-05-30
申请号：US387117
申请日：1999-08-31
申请人： Hideaki Tanaka , Yoshikazu Amano
发明人： Hideaki Tanaka , Yoshikazu Amano
IPC分类号： C12Q1/32 , C12Q1/527 , C12Q1/00 , C12Q1/48
CPC分类号： C12Q1/32 , C12Q1/527
摘要： This invention provides a reagent composition for the determination of bicarbonate ion capable of measuring in a wide determination range in visible light region which comprises a combined substrate of thioNAD(P)H and NAD(P)H for a coupled enzymes of phosphoenolpyruvate carboxylase and malate dehydrogenase.
摘要翻译：本发明提供了一种用于测定碳酸氢根离子的试剂组合物，其能够在可见光区域的广泛测定范围内测量，所述可见光区域包含硫代纳（P）H和NAD（P）H的组合底物，用于磷酸烯醇丙酮酸羧化酶和苹果酸的偶联酶脱氢酶。

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