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1. 发明授权

US07820402B2 Immunoassay element 失效
标题翻译：免疫测定元件
公开(公告)号：US07820402B2
公开(公告)日：2010-10-26
申请号：US11230127
申请日：2005-09-19
申请人： Yoshiki Sakaino , Hitomi Ito , Toshihiro Mori , Osamu Seshimoto , Toshihisa Ito , Yoshikazu Amano
发明人： Yoshiki Sakaino , Hitomi Ito , Toshihiro Mori , Osamu Seshimoto , Toshihisa Ito , Yoshikazu Amano
IPC分类号： G01N33/53 , G01N33/543
CPC分类号： G01N33/54386 , G01N2333/924 , Y10S435/966 , Y10S435/969 , Y10S435/97 , Y10S436/807 , Y10S436/81
摘要： The immunoassay element for quantitatively analyzing an antigen by determining the change in enzymatic activity of an enzyme-labelled antigen or antibody caused by an immunological reaction. The immunoassay element comprises a substrate layer containing a non-diffusible substrate which forms a diffusible material in the presence of the labelling enzyme, and a reagent layer containing a fragmenting enzyme for further fragmenting the diffusible material into a lower molecular weight product. As the non-diffusible substrate, a substrate capable of reacting solely with the labelling enzyme and incapable of reacting the fragmenting enzyme is utilized. When an endo-active glucosidase is used as the labelling enzyme, and an exo-active glucosidase is used the fragmenting enzyme in the reagent layer, the non-diffusible substrate of the substrate layer is preferred to be an endo type selectively reactive substrate, which means a substrate having a reactivity specific to endo-active glucosidase. Highly sensitive assay is realized with high accuracy and high reproducibility and good storage stability.
摘要翻译：用于通过确定由免疫反应引起的酶标记的抗原或抗体的酶活性的变化来定量分析抗原的免疫测定元件。免疫测定元件包括含有在标记酶存在下形成可扩散材料的不扩散基底的底物层和含有用于进一步将可扩散材料分解成较低分子量产物的碎裂酶的试剂层。作为非扩散性底物，使用能够仅与标记酶反应并且不能使片段化酶反应的底物。当使用内源活性葡糖苷酶作为标记酶，并且使用外源活性葡糖苷酶时，试剂层中的断裂酶，底物层的非扩散性基质优选为内型选择性反应性底物，其中是指具有对内源性葡糖苷酶特异性的反应性的底物。高精度，高重现性和良好的储存稳定性实现了高灵敏度测定。

2. 发明申请

US20060024770A1 Immunoassay element 失效
公开(公告)号：US20060024770A1
公开(公告)日：2006-02-02
申请号：US11230127
申请日：2005-09-19
申请人： Yoshiki Sakaino , Hitomi Ito , Toshihiro Mori , Osamu Seshimoto , Toshihisa Ito , Yoshikazu Amano
发明人： Yoshiki Sakaino , Hitomi Ito , Toshihiro Mori , Osamu Seshimoto , Toshihisa Ito , Yoshikazu Amano
IPC分类号： G01N33/537 , G01N33/53 , G01N33/543
CPC分类号： G01N33/54386 , G01N2333/924 , Y10S435/966 , Y10S435/969 , Y10S435/97 , Y10S436/807 , Y10S436/81
摘要： The immunoassay element for quantitatively analyzing an antigen by determining the change in enzymatic activity of an enzyme-labelled antigen or antibody caused by an immunological reaction. The immunoassay element comprises a substrate layer containing a non-diffusible substrate which forms a diffusible material in the presence of the labelling enzyme, and a reagent layer containing a fragmenting enzyme for further fragmenting the diffusible material into a lower molecular weight product. As the non-diffusible substrate, a substrate capable of reacting solely with the lebelling enzyme and incapable of reacting the fragmenting enzyme is utilized. When an endo-active glucosidase is used as the labelling enzyme, and an exo-active glucosidase is used the fragmenting enzyme in the reagent layer, the non-diffusible substrate of the substrate layer is preferred to be an endo type selectively reactive substrate, which means a substrate having a reactivity specific to endo-active glucosidase. Highly sensitive assay is realized with high accuracy and high reproducibility and good storage stability.

3. 发明申请

US20050084953A1 Immunoassay element 审中-公开
标题翻译：免疫测定元件
公开(公告)号：US20050084953A1
公开(公告)日：2005-04-21
申请号：US10961459
申请日：2004-10-07
申请人： Yoshiki Sakaino , Hitomi Ito , Toshihiro Mori , Osamu Seshimoto , Toshihisa Ito , Yoshikazu Amano
发明人： Yoshiki Sakaino , Hitomi Ito , Toshihiro Mori , Osamu Seshimoto , Toshihisa Ito , Yoshikazu Amano
IPC分类号： G01N33/543 , G01N33/53
CPC分类号： G01N33/54386 , G01N2333/924 , Y10S435/966 , Y10S435/969 , Y10S435/97 , Y10S436/807 , Y10S436/81
摘要： The immunoassay element for quantitatively analyzing an antigen by determining the change in enzymatic activity of an enzyme-labelled antigen or antibody caused by an immunological reaction. The immunoassay element comprises a substrate layer containing a non-diffusible substrate which forms a diffusible material in the presence of the labelling enzyme, and a reagent layer containing a fragmenting enzyme for further fragmenting the diffusible material into a lower molecular weight product. As the non-diffusible substrate, a substrate capable of reacting solely with the lebelling enzyme and incapable of reacting the fragmenting enzyme is utilized. When an endo-active glucosidase is used as the labelling enzyme, and an exo-active glucosidase is used the fragmenting enzyme in the reagent layer, the non-diffusible substrate of the substrate layer is preferred to be an endo type selectively reactive substrate, which means a substrate having a reactivity specific to endo-active glucosidase. Highly sensitive assay is realized with high accuracy and high reproducibility and good storage stability.
摘要翻译：用于通过确定由免疫反应引起的酶标记的抗原或抗体的酶活性的变化来定量分析抗原的免疫测定元件。免疫测定元件包括含有在标记酶存在下形成可扩散材料的不扩散基底的底物层和含有用于进一步将可扩散材料分解成较低分子量产物的碎裂酶的试剂层。作为非扩散性底物，可以使用能够仅与前体酶反应并且不能使片段化酶反应的底物。当使用内源活性葡糖苷酶作为标记酶，并且使用外源活性葡糖苷酶时，试剂层中的断裂酶，底物层的非扩散性基质优选为内型选择性反应性底物，其中是指具有对内源性葡糖苷酶特异性的反应性的底物。高精度，高重现性和良好的储存稳定性实现了高灵敏度测定。

4. 发明申请

US20050186110A1 Multilayer analysis element 审中-公开
标题翻译：多层分析单元
公开(公告)号：US20050186110A1
公开(公告)日：2005-08-25
申请号：US11060777
申请日：2005-02-18
申请人： Kentaro Nakamura , Kazuya Kawasaki , Toshihisa Ito , Yoshiki Sakaino , Osamu Seshimoto
发明人： Kentaro Nakamura , Kazuya Kawasaki , Toshihisa Ito , Yoshiki Sakaino , Osamu Seshimoto
IPC分类号： G01N31/22
CPC分类号： G01N31/22
摘要： It is an object of the invention to provide a multilayer analysis element with small intra-lot and lot-to-lot differences that has high measurement accuracy and which is small in size. The present invention provides a multilayer analysis element for the analysis of a liquid sample, comprising a water impermeable planar support on one side of which at least one functional layer and at least one porous liquid sample spreading layer of non-fibrous porous film are integrally laminated in the mentioned order, wherein said spreading layer comprises a non-fibrous porous film such that, when a liquid sample containing a target substance to be measured is caused to become attached to the surface of said liquid sample spreading layer, where said liquid sample is spread and dispersed, 30% or more of said target substance to be measured can be transferred to the at least one functional layer below said liquid sample spreading layer.
摘要翻译：本发明的目的是提供一种具有很小的批量间和批间差异的多层分析元件，其具有高的测量精度并且尺寸小。本发明提供了一种用于分析液体样品的多层分析元件，其包括不透水平面支撑体，其一侧上至少一个功能层和至少一个非纤维多孔膜的多孔液体样品铺展层整体层压其中所述扩散层包括非纤维多孔膜，使得当将含有待测物体的液体样品附着在所述液体样品扩散层的表面上时，其中所述液体样品为扩散和分散，所述待测量的所述目标物质的30％以上可以转移到所述液体样品扩散层下方的至少一个功能层。

5. 发明申请

US20080019869A1 Multilayer Analysis Element 审中-公开
标题翻译：多层分析单元
公开(公告)号：US20080019869A1
公开(公告)日：2008-01-24
申请号：US10589556
申请日：2005-02-18
申请人： Kentaro Nakamura , Kazuya Kawasaki , Yoshiki Sakaino , Toshihisa Ito , Osamu Seshimoto
发明人： Kentaro Nakamura , Kazuya Kawasaki , Yoshiki Sakaino , Toshihisa Ito , Osamu Seshimoto
IPC分类号： B01L11/00
CPC分类号： G01N33/525
摘要： It is an object of the invention to provide a multilayer analysis element with small intra-lot and lot-to-lot differences that has high measurement accuracy and which is small in size. The present invention provides a multilayer analysis element for liquid sample analysis wherein at least one functional layer and at least one porous liquid sample spreading layer of non-fibrous porous film are integrally laminated in this order on one side of a water-impermeable planar support, and the non-fibrous porous film has a bending rupture strength of 20 gram-weight or more and a tensile percentage of 2% or less when the film is stretched with a tensile force of 50 gram-weight.
摘要翻译：本发明的目的是提供一种具有很小的批量间和批间差异的多层分析元件，其具有高的测量精度并且尺寸小。本发明提供了一种用于液体样品分析的多层分析元件，其中至少一个功能层和非纤维多孔膜的至少一个多孔液体样品扩散层在不透水平面支撑体的一侧上依次一体地层叠，并且当以50克重的拉伸力拉伸膜时，非纤维状多孔膜的弯曲断裂强度为20克重以上，拉伸率为2％以下。

6. 发明申请

US20050186109A1 Multilayer analysis element 审中-公开
标题翻译：多层分析单元
公开(公告)号：US20050186109A1
公开(公告)日：2005-08-25
申请号：US11060775
申请日：2005-02-18
申请人： Kentaro Nakamura , Kazuya Kawasaki , Yoshiki Sakaino , Toshihisa Ito , Osamu Seshimoto
发明人： Kentaro Nakamura , Kazuya Kawasaki , Yoshiki Sakaino , Toshihisa Ito , Osamu Seshimoto
IPC分类号： G01N31/22
CPC分类号： G01N31/22
摘要： An object of the invention to provide a multilayer analysis element with small intra-lot and lot-to-lot differences that has high measurement accuracy and which is small in size. The present invention provides a multilayer analysis element for the analysis of a liquid sample, comprising a water impermeable planar support on one side of which at least one functional layer and at least one porous liquid sample spreading layer of non-fibrous porous film are integrally laminated in the mentioned order, wherein the arithmetic mean deviation of the profile (Ra) of a contact surface of the at least one functional layer that is in contact with said porous liquid sample spreading layer of said non-fibrous porous film is 1 μm or less and/or the ten-point height of irregularities (Rz) of said contact surface is 8 μm or less.
摘要翻译：本发明的目的是提供一种具有很高的测量精度并且尺寸小的多批次间差异小的多层分析元件。本发明提供了一种用于分析液体样品的多层分析元件，其包括不透水平面支撑体，其一侧上至少一个功能层和至少一个非纤维多孔膜的多孔液体样品铺展层整体层压其中与所述非纤维多孔膜的所述多孔液体样品扩散层接触的所述至少一个功能层的接触表面的轮廓（Ra）的算术平均偏差为1μm以下和/或所述接触表面的十点高度的不规则（Rz）为8μm或更小。

7. 发明授权

US07105128B2 Dry analytical element using water-soluble indicator for colorimetry 有权
公开(公告)号：US07105128B2
公开(公告)日：2006-09-12
申请号：US10281564
申请日：2002-10-28
申请人： Hideaki Tanaka , Yoshihide Iwaki , Yoshiki Sakaino , Kazuya Kawasaki , Yoshikazu Amano
发明人： Hideaki Tanaka , Yoshihide Iwaki , Yoshiki Sakaino , Kazuya Kawasaki , Yoshikazu Amano
IPC分类号： G01N31/22
CPC分类号： G01N33/525 , G01N33/84
摘要： This invention relates to a dry analytical element using a water-soluble indicator which improves measuring accuracy, which comprises a water-impermeable support, a water-permeable layer and a spreading layer having a function to spread a liquid uniformly, wherein a water-soluble indicator for colorimetry is incorporated into the s preading layer.

8. 发明申请

US20060016747A1 Glass-fiber filter for blood filtration, blood filtration device and blood analysis element 审中-公开
标题翻译：用于血液过滤的玻璃纤维过滤器，血液过滤装置和血液分析元件
公开(公告)号：US20060016747A1
公开(公告)日：2006-01-26
申请号：US11185815
申请日：2005-07-21
申请人： Yoshiki Sakaino , Toshihisa Ito
发明人： Yoshiki Sakaino , Toshihisa Ito
IPC分类号： B01D35/31
CPC分类号： B01D39/2017 , B01D2239/0492
摘要： A glass fiber filter for blood filtration, in which a glass fiber is cleaned with an organic acid and then the surface of a glass fiber is coated with a biocompatible polymer such as poly (alkoxy acrylate), a blood filtration device and a dry-type blood analysis element in which the glass fiber filter for blood filtration is used.
摘要翻译：一种用于血液过滤的玻璃纤维过滤器，其中用有机酸清洁玻璃纤维，然后用玻璃纤维的表面涂覆生物相容性聚合物，例如聚（烷氧基丙烯酸酯），血液过滤装置和干式血液分析元件，其中使用用于血液过滤的玻璃纤维过滤器。

9. 发明申请

US20070221563A1 Nucleic Acid-Adsorbing Porous Membrane for Separating and Purifying Nucleic Acid and Apparatus for Separating and Purifying Nucleic Acid 审中-公开
标题翻译：用于分离和纯化核酸的核酸吸附多孔膜和用于分离和纯化核酸的装置
公开(公告)号：US20070221563A1
公开(公告)日：2007-09-27
申请号：US10576418
申请日：2004-10-20
申请人： Yoshiki Sakaino , Yoshihiko Makino , Toshihiro Mori
发明人： Yoshiki Sakaino , Yoshihiko Makino , Toshihiro Mori
IPC分类号： B01D63/00
CPC分类号： C12N15/1017 , B01L3/502 , B01L9/06 , B01L2200/0631 , B01L2300/0681 , B01L2400/0487 , G01N1/34 , G01N1/405
摘要： To provide a nucleic acid-adsorbing porous membrane which has an excellent separating ability, which provides a good washing efficiency, which permits convenient and expeditious procedures, which is adapted for automation and reduction in size, which can be mass produced with substantially identical separating capability, and which is adapted for a method of separating and purifying nucleic acids and to provide an apparatus using the same, a nucleic acid-adsorbing porous membrane for separating and purifying a nucleic acid, comprises a nucleic acid-adsorbing solid phase for use in a method for separating and purifying the nucleic acid, the solid phase adsorbing the nucleic acid, the method comprising the steps of: (1) adsorbing the nucleic acid to the solid phase by allowing a sample solution containing the nucleic acid to come into contact with the nucleic acid-adsorbing solid phase; (2) washing the solid phase by allowing a washing solution to come into contact with the solid phase, while the nucleic acid is adsorbed to the solid phase; and (3) desorbing the nucleic acid from the solid phase by allowing a recovering solution to come into contact with the solid phase.
摘要翻译：为了提供具有优异的分离能力的核酸吸附多孔膜，其提供了良好的洗涤效率，其允许方便和快速的程序，其适于自动化和尺寸减小，其可以基本相同的分离能力大量生产并且其适用于分离和纯化核酸的方法，并提供使用该方法的装置，用于分离和纯化核酸的核酸吸附多孔膜包括用于在核酸中的核酸吸附固相用于分离和纯化核酸的方法，所述固相吸附核酸，所述方法包括以下步骤：（1）通过使含有所述核酸的样品溶液与所述核酸接触来吸附所述核酸至所述固相核酸吸附固相; （2）通过使洗涤液与固相接触来洗涤固相，同时核酸被吸附到固相上; 和（3）通过使回收溶液与固相接触从固相中解吸核酸。

10. 发明申请

US20070178009A1 Analysis element for use in method of testing specimen 审中-公开
标题翻译：分析元素用于测试样品的方法
公开(公告)号：US20070178009A1
公开(公告)日：2007-08-02
申请号：US10592230
申请日：2005-03-15
申请人： Yoshiki Sakaino , Yoshihiko Abe , Yukio Sudo
发明人： Yoshiki Sakaino , Yoshihiko Abe , Yukio Sudo
IPC分类号： G01N21/00
CPC分类号： A61B5/157 , A61B5/14532 , A61B5/14546 , A61B5/1455 , A61B5/1486 , A61B5/150022 , A61B5/150099 , A61B5/150274 , A61B5/150343 , A61B5/150358 , A61B5/150389 , A61B2560/0223 , A61B2562/0295 , G01N21/31 , G01N21/4738 , G01N21/75 , G01N31/22
摘要： An analysis element for use in a blood test method enabling easy and simple operations that are performed quickly up to measurement, an analysis element for use in a blood test method that operations up to the measurement for many components can be quickly performed, and that is safe and has a sufficient measurement accuracy thereof, and an analysis element for use in a test method using body fluids and urines of humans and animals, plain water, seawater, soil extract, agricultural products, marine products, processed-food extracts, and liquid for use in scientific research as specimens, are provided, the present invention relates to a multi-component measurement dry analysis element for use in a method for testing a specimen, the method using an area sensor as a detector to obtain a result of measurement according to information represented by 1000 pixels or more per one component, and to perform simultaneous measurements of plural components.
摘要翻译：一种用于血液检查方法的分析元件，其能够实现快速进行测量的简单且简单的操作，用于血液测试方法的分析元件可以快速执行多达成分测量的操作，也就是说安全且具有足够的测量精度，以及用于使用人体和动物，普通水，海水，土壤提取物，农产品，海产品，加工食品提取物和液体的体液和尿液的测试方法中的分析元件本发明涉及一种用于试样检测方法的多组分测量干燥分析元件，该方法使用区域传感器作为检测器，以获得测量结果对于每个分量由1000个像素或更多个表示的信息，并且执行多个分量的同时测量。

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