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71. 发明申请

US20090152114A1 Complex formation method and separation method 有权
标题翻译：复杂形成方法和分离方法
公开(公告)号：US20090152114A1
公开(公告)日：2009-06-18
申请号：US11991170
申请日：2006-08-24
申请人： Tomohisa Kawabata , Shinji Satomura , Henry Garrett Wada
发明人： Tomohisa Kawabata , Shinji Satomura , Henry Garrett Wada
IPC分类号： C25B7/00
CPC分类号： G01N33/561 , C12M23/16 , C12M45/07 , G01N33/5306 , G01N33/536 , G01N33/5375 , G06F19/18 , G06F19/702 , Y10S977/705
摘要： An object of the present invention is to provide a method for forming a complex between an analyte or an analogue thereof, and a substance formable the complex with said analyte or said analogue thereof (the complex forming substance), in a short time and in high reaction efficiency, and a method for separating a complex formed, and a complex forming substance not involved in formation of said complex or an analogue not involved in formation of said complex rapidly, simply and in high accuracy, along with a method for measuring an analyte in a sample in high sensitivity.
摘要翻译：本发明的目的是提供一种在分析物或其类似物之间形成复合物的方法，以及在短时间内和高温下与所述分析物或其类似物（复合物形成物质）形成复合物的物质反应效率的方法和形成的络合物的方法以及不涉及所述络合物的形成的复合物形成物质或不涉及快速形成所述复合物的类似物，以及用于测定分析物的方法在高灵敏度的样品中。

72. 发明申请

US20080280281A1 METHOD OF ALTERING THE BINDING SPECIFICITY OF PLASMA PROTEINS BY OXIDATION-REDUCTION REACTIONS 有权
标题翻译：通过氧化还原反应改变等离子体蛋白的结合特性的方法
公开(公告)号：US20080280281A1
公开(公告)日：2008-11-13
申请号：US12107819
申请日：2008-04-23
申请人： John A. McIntyre
发明人： John A. McIntyre
IPC分类号： A01N1/02 , C07K1/00 , G01N33/53 , A61M1/00
CPC分类号： C07K16/00 , C07K16/18 , C07K2317/21 , G01N33/5306 , G01N33/5375 , G01N33/564 , G01N33/6854 , Y10S424/81 , Y10S436/825 , Y10S530/868
摘要： The binding specificity of at least one plasma protein suspended or dissolved in a liquid medium is altered by exposing the protein to an oxidizing agent or an electric current sufficient to alter its binding specificity. A masked protein such as an autoantibody can be recovered from blood or blood products or extracts by oxidizing the protein to change its binding specificity.
摘要翻译：通过将蛋白质暴露于氧化剂或足以改变其结合特异性的电流来改变悬浮或溶解在液体培养基中的至少一种血浆蛋白的结合特异性。通过氧化蛋白质以改变其结合特异性，可以从血液或血液制品或提取物中回收诸如自身抗体的掩蔽蛋白质。

73. 发明申请

US20060177437A1 Binding peptides: methods for their generation and use 审中-公开
标题翻译：结合肽：其生成和使用的方法
公开(公告)号：US20060177437A1
公开(公告)日：2006-08-10
申请号：US11299288
申请日：2005-12-09
申请人： Erwin Houtzager , Wietse Willebrands , Guy de Roo , KeesJan Francoijs , Irma Vijn
发明人： Erwin Houtzager , Wietse Willebrands , Guy de Roo , KeesJan Francoijs , Irma Vijn
IPC分类号： A61K39/395 , C07K16/10 , C07K16/12 , C07K16/40
CPC分类号： C07K1/107 , G01N33/5375
摘要： Described are means and methods for generating binding peptide associated with a suitable core region, the resulting proteinaceous molecule and uses thereof. The invention provides a solution to the problems associated with the use of binding molecules over their entire range of use. Binding molecules can be designed to accommodate extreme conditions of use, such as extreme temperatures or pH. Alternatively, binding molecules can be designed to respond to very subtle changes in the environment.
摘要翻译：描述了产生与合适核心区域相关的结合肽，所得蛋白质分子及其用途的方法和方法。本发明提供了在整个使用范围内与使用结合分子相关的问题的解决方案。结合分子可以设计成适应极端的使用条件，如极端温度或pH值。或者，可以设计结合分子以响应环境中非常微妙的变化。

74. 发明授权

US07056682B2 Immunoassay method and immunoassay reagent kit to be used therein 有权
标题翻译：免疫测定法和免疫测定试剂盒用于其中
公开(公告)号：US07056682B2
公开(公告)日：2006-06-06
申请号：US10474755
申请日：2002-12-27
申请人： Akihito Kamei , Noriko Kenjo , Tatsuro Kawamura , Mahito Hirai
发明人： Akihito Kamei , Noriko Kenjo , Tatsuro Kawamura , Mahito Hirai
IPC分类号： G01N33/53
CPC分类号： G01N33/5375 , G01N33/536 , G01N33/539 , G01N33/542
摘要： In step St1 shown in FIG. 1, a reaction system including a sample for which the content of a subject substance is to be measured, a specific-binding substance specifically binding to the subject substance, and phthalic acid or phthalate salt is constructed. The pH of the reaction system is set at less than 7. When the subject substance is an antigen, the specific-binding substance is an antibody. In reverse, when the subject substance is an antibody, the specific-binding substance is an antigen. In step St2 in FIG. 1, an optical property of the reaction system is measured. When agglutinate complexes are produced in the step St1, the reaction system becomes turbid, causing a change in scattered light intensity, transmitted light amount and the like. Therefore, by measuring the scattered light intensity, the transmitted light amount or the like, the degree of turbidity of the reaction system can be estimated.
摘要翻译：在图1所示的步骤St 1中，如图1所示，构成了包含被测定物质含量的样品，特异性结合物质的特异性结合物和邻苯二甲酸或邻苯二甲酸盐的反应体系。反应体系的pH设定为小于7.当目标物质为抗原时，特异性结合物质为抗体。相反，当目标物质是抗体时，特异性结合物质是抗原。在图3的步骤St2中如图1所示，测定反应体系的光学特性。当在步骤St 1中产生凝集络合物时，反应体系变得混浊，导致散射光强度，透射光量等的变化。因此，通过测量散射光强度，透射光量等，可以估计反应体系的浊度。

75. 发明申请

US20050048474A1 Process and apparatus for separating and recovering particles from a liquid sample 审中-公开
标题翻译：用于从液体样品中分离和回收颗粒的方法和设备
公开(公告)号：US20050048474A1
公开(公告)日：2005-03-03
申请号：US10920992
申请日：2004-08-18
申请人： James Amburgey
发明人： James Amburgey
IPC分类号： C12M1/12 , C12Q1/04 , C12Q1/24 , C12Q1/70 , G01N33/537 , G01N33/539
CPC分类号： G01N33/5375 , C12M47/02 , C12Q1/24 , G01N33/539
摘要： The process and apparatus of this invention can be used to separate and recover particles from a liquid sample. The preferred embodiment of this invention is to separate and recover microorganisms, particularly Cryptosporidium oocysts, from a variety of environmental sources. The process consists of applying a municipal drinking water coagulation scheme to an environmental water sample, and the aforementioned water sample is then filtered through an apparatus containing a granular filter material. The analyte is then recovered by agitating the filter vigorously (with a dispersing agent inside) and removing the eluent from the filtration apparatus. The eluent can then by aliquotted and assayed by any means available. The floc resulting from the coagulant addition can typically be dissolved at low pH. The filter apparatus is simply a container that allows the passage of water through a granular material while retaining the granular material via a mesh, screen, or similar material.
摘要翻译：本发明的方法和装置可用于从液体样品中分离和回收颗粒。本发明的优选实施方案是从各种环境来源分离和回收微生物，特别是隐孢子虫卵囊。该方法包括将市政饮用水凝结方案应用于环境水样品，然后通过含有粒状过滤材料的装置过滤上述水样品。然后通过剧烈搅拌过滤器（使用分散剂）并从过滤装置中除去洗脱液来回收分析物。然后可以通过任何可用的方法将洗脱液等分和测定。凝结剂添加产生的絮凝物通常可以在低pH下溶解。过滤装置简单地是允许水通过颗粒材料的容器，同时通过网状物，筛网或类似材料保持颗粒材料。

76. 发明授权

US5635362A Assay of blood or other biologic samples for target analytes 失效
标题翻译：测定目标分析物的血液或其他生物样品
公开(公告)号：US5635362A
公开(公告)日：1997-06-03
申请号：US247336
申请日：1994-05-23
申请人： Robert A. Levine , Stephen C. Wardlaw , Rodolfo R. Rodriguez , Adrien P. Malick , Alvydas J. Ozinskas
发明人： Robert A. Levine , Stephen C. Wardlaw , Rodolfo R. Rodriguez , Adrien P. Malick , Alvydas J. Ozinskas
IPC分类号： G01N33/543 , B01L3/14 , G01N33/49 , G01N33/537 , G01N33/569 , G01N33/58 , G01N33/558
CPC分类号： B01L3/5021 , G01N33/491 , G01N33/5375 , G01N33/56972 , G01N33/585 , Y10S435/81 , Y10S435/967 , Y10S435/971 , Y10S435/973 , Y10S436/805 , Y10S436/81 , Y10S436/824 , Y10S436/829 , Y10T436/111666
摘要： A patient's health may be diagnosed by centrifuging blood samples in a transparent tube, which tube contains one or more bodies or groups of bodies such as floats, inserts, liposomes, or plastic beads of different densities. Each density-defined body carries analyte-capture binding materials such as antigens or antibodies, which are specific to an epitope, or other specific high affinity binding site on a target analyte which target analyte may be in the blood or other sample being tested; and the level of which analyte is indicative of the patient's health. At least one labeled binding material which is also specific to an epitope, or other specific high affinity binding site on the target analyte is added to the sample so as to form labeled binding material/analyte/body complexes in the sample. Upon centrifugation, the complexes will settle out in different areas in the tube according to the respective density of the body or bodies; and the degree of label emission of the complex layers can enable qualitative and/or quantitative analyses of the sample to be made. Unbound labeled binding materials will be separated from the complexed layers by the washing action of ascending or descending components of the sample during the centrifugation step. Unbound labeled binding material will thus not interfere with the analysis.
摘要翻译：可以通过将透明管中的血液样品离心来诊断患者的健康，该管包含一个或多个不同密度的浮体，插入物，脂质体或塑料珠的主体或组。每个密度定义的身体携带分析物 - 捕获结合材料，例如抗原或抗体，其对靶分析物是特异性的，或靶分析物上的其它特异性高亲和力结合位点，其目标分析物可能在待测试的血液或其他样品中; 并且其分析物的水平表示患者的健康。将至少一种对靶分析物上的表位或其他特异性高亲和力结合位点特异性的标记结合物质加入到样品中，以便在样品中形成标记的结合材料/分析物/身体复合物。离心后，复合物将根据身体或身体的相应密度沉淀在管中的不同区域; 并且复合层的标签发射程度可以使得要进行样品的定性和/或定量分析。通过在离心步骤期间样品的上升或下降组分的洗涤作用，未结合的标记结合材料将从复合层分离。因此，未结合的标签结合材料不会影响分析。

77. 发明授权

US5460979A Indirect fluorescent assay of blood samples 失效
标题翻译：血液样品的间接荧光检测
公开(公告)号：US5460979A
公开(公告)日：1995-10-24
申请号：US192629
申请日：1994-02-07
申请人： Robert A. Levine , Stephen C. Wardlaw , Leon W. M. M. Terstappen , Thomas J. Mercolino , Diether J. Recktenwald
发明人： Robert A. Levine , Stephen C. Wardlaw , Leon W. M. M. Terstappen , Thomas J. Mercolino , Diether J. Recktenwald
IPC分类号： G01N33/543 , B01L3/14 , G01N33/49 , G01N33/537 , G01N33/569 , G01N33/58 , G01N33/538 , G01N33/546
CPC分类号： B01L3/5021 , G01N33/491 , G01N33/5375 , G01N33/56972 , G01N33/585 , Y10S435/81 , Y10S435/967 , Y10S435/971 , Y10S435/973 , Y10S436/805 , Y10S436/81 , Y10S436/824 , Y10S436/829 , Y10T436/111666
摘要： A patient's health is diagnosed by centrifuging blood samples in a transparent tube, which tube contains one or more groups of particles such as lyposomes or plastic beads of different densities for each group. Each group of density-defined particles carries antigens or antibodies which are specific to a complement antigen or antibody which may be in the blood sample being tested, and which are indicative of the patient's health. A label-tagged antibody which is specific to all bound antibody/antigen couples is added to the blood sample so as to form labelled antibody+antigen-antibody complexes (AAAC) in the blood sample. Upon centrifugation, the complexed particles will settle out in different areas in the tube according to the respective density of the particles, and the degree of label emission of the particle layers can enable qualitative or quantitative analyses of the blood sample to be made. Unbound labelled antibodies will be washed away from the complexed layers by the washing action of the descending blood cells during the centrifugation step. Unbound labelled antibodies will thus not interfere with the analysis.
摘要翻译：通过在透明管中离心血液样品来诊断患者的健康，该管含有一组或多组颗粒，例如每组的不同密度的溶血细胞或塑料珠。每组密度定义的颗粒携带对可能在被测试的血液样品中的补体抗原或抗体特异性的抗原或抗体，并且其表示患者的健康。向血液样品中加入对所有结合的抗体/抗原对特异性的标记标签抗体，以在血液样品中形成标记抗体+抗原 - 抗体复合物（AAAC）。离心后，根据颗粒的相应密度，络合的颗粒将沉淀在管中的不同区域中，并且颗粒层的标签发射程度可以使得要进行血液样品的定性或定量分析。在离心步骤中，未结合的标记抗体将通过下降血细胞的洗涤作用从复合层中洗去。因此，未结合的标记抗体不会干扰分析。

78. 发明授权

US4962047A Mixing and separating solid phase supports by pressure variation 失效
标题翻译：通过压力变化混合和分离固相载体
公开(公告)号：US4962047A
公开(公告)日：1990-10-09
申请号：US924033
申请日：1986-10-28
申请人： John F. Place
发明人： John F. Place
IPC分类号： C12N11/00 , G01N33/537 , G01N33/543 , G01N33/544
CPC分类号： G01N33/5375 , G01N33/54313 , Y10S435/803 , Y10S435/814 , Y10S436/824 , Y10S530/81
摘要： Gas containing granules of a resiliently compressible material having a density variable with pressure are used as a solid phase support in liquid phase reactions such in immunological reactions. Varying pressure causes the density of the granules to change and is used for mixing and separating the granules in liquid phase. In an immunological reaction, the granules having an attached antibody are combined with a solution containing an antigen, then varying pressure is applied to the solution to mix the granules in the solution to fix the antigen to the antibody and then pressure on the solution is varied to force the granules to the top or bottom of the solution whereby the solution can be separated from the granules.
摘要翻译：使用具有密度随压力变化变化的可弹性压缩材料的含气颗粒作为液相反应中的固相载体，如免疫反应。变化的压力导致颗粒的密度改变，并用于混合和分离液相中的颗粒。在免疫反应中，将具有附着抗体的颗粒与含有抗原的溶液合并，然后对溶液施加变化的压力以将颗粒混合在溶液中以将抗原固定在抗体上，然后对溶液施加压力变化以迫使颗粒到溶液的顶部或底部，从而使溶液与颗粒分离。

79. 发明授权

US11899013B2 Method of detecting or quantifying detection target in specimen, composite particle, and reagent 有权
公开(公告)号：US11899013B2
公开(公告)日：2024-02-13
申请号：US16910148
申请日：2020-06-24
申请人： Canon Medical Systems Corporation
发明人： Satoru Sugita , Hirotoshi Tahara
IPC分类号： G01N33/551 , G01N33/542 , G01N33/543 , G01N21/75 , G01N21/17 , G01N25/18 , G01N25/48 , G01N23/2273 , G01N33/538 , G01N33/537
CPC分类号： G01N33/551 , G01N21/17 , G01N21/75 , G01N25/18 , G01N25/482 , G01N33/542 , G01N33/54313 , G01N23/2273 , G01N33/538 , G01N33/5375
摘要： According to one embodiment, a method of detecting or quantifying a detection target in a specimen includes: irradiating a reaction mixture containing composite particles and the specimen with light to promote binding between the composite particles and the detection target; and performing measurement on the reaction mixture irradiated with the light to detect or quantify the detection target. The composite particles each include a carrier particle including two or more regions having different physical properties on a surface, and an affinity substance carried on the carrier particle and having affinity to the detection target. The light can be absorbed by at least one of the two or more regions.

80. 发明公开

US20230417743A1 ANTIBODY-LINKED IMMUNO-SEDIMENTATION AGENT AND METHOD OF ISOLATING A TARGET FROM A SAMPLE USING SAME 审中-公开
公开(公告)号：US20230417743A1
公开(公告)日：2023-12-28
申请号：US18241668
申请日：2023-09-01
申请人： CytoSed, Inc.
发明人： Warren L. Dinges
IPC分类号： G01N33/539 , G01N33/569 , C07K16/00 , G01N33/537
CPC分类号： G01N33/539 , G01N33/56966 , G01N33/56988 , C07K16/00 , G01N33/56972 , G01N33/5375 , G01N2333/7051
摘要： The present disclosure is directed to antibody-linked immuno-sedimentation agent, the antibody being linked to a sedimentation agent by a non-antigen binding region of the antibody, and a method of isolating a target from a sample using the antibody-linked immuno-sedimentation agent. The methods involve forming a mixture including a sample with an antibody linked immuno-sedimentation agent and red blood cells under conditions sufficient to form red blood cell rouleaux and allow antibody-antigen binding.

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