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1. 发明授权

US5635362A Assay of blood or other biologic samples for target analytes 失效
标题翻译：测定目标分析物的血液或其他生物样品
公开(公告)号：US5635362A
公开(公告)日：1997-06-03
申请号：US247336
申请日：1994-05-23
申请人： Robert A. Levine , Stephen C. Wardlaw , Rodolfo R. Rodriguez , Adrien P. Malick , Alvydas J. Ozinskas
发明人： Robert A. Levine , Stephen C. Wardlaw , Rodolfo R. Rodriguez , Adrien P. Malick , Alvydas J. Ozinskas
IPC分类号： G01N33/543 , B01L3/14 , G01N33/49 , G01N33/537 , G01N33/569 , G01N33/58 , G01N33/558
CPC分类号： B01L3/5021 , G01N33/491 , G01N33/5375 , G01N33/56972 , G01N33/585 , Y10S435/81 , Y10S435/967 , Y10S435/971 , Y10S435/973 , Y10S436/805 , Y10S436/81 , Y10S436/824 , Y10S436/829 , Y10T436/111666
摘要： A patient's health may be diagnosed by centrifuging blood samples in a transparent tube, which tube contains one or more bodies or groups of bodies such as floats, inserts, liposomes, or plastic beads of different densities. Each density-defined body carries analyte-capture binding materials such as antigens or antibodies, which are specific to an epitope, or other specific high affinity binding site on a target analyte which target analyte may be in the blood or other sample being tested; and the level of which analyte is indicative of the patient's health. At least one labeled binding material which is also specific to an epitope, or other specific high affinity binding site on the target analyte is added to the sample so as to form labeled binding material/analyte/body complexes in the sample. Upon centrifugation, the complexes will settle out in different areas in the tube according to the respective density of the body or bodies; and the degree of label emission of the complex layers can enable qualitative and/or quantitative analyses of the sample to be made. Unbound labeled binding materials will be separated from the complexed layers by the washing action of ascending or descending components of the sample during the centrifugation step. Unbound labeled binding material will thus not interfere with the analysis.
摘要翻译：可以通过将透明管中的血液样品离心来诊断患者的健康，该管包含一个或多个不同密度的浮体，插入物，脂质体或塑料珠的主体或组。每个密度定义的身体携带分析物 - 捕获结合材料，例如抗原或抗体，其对靶分析物是特异性的，或靶分析物上的其它特异性高亲和力结合位点，其目标分析物可能在待测试的血液或其他样品中; 并且其分析物的水平表示患者的健康。将至少一种对靶分析物上的表位或其他特异性高亲和力结合位点特异性的标记结合物质加入到样品中，以便在样品中形成标记的结合材料/分析物/身体复合物。离心后，复合物将根据身体或身体的相应密度沉淀在管中的不同区域; 并且复合层的标签发射程度可以使得要进行样品的定性和/或定量分析。通过在离心步骤期间样品的上升或下降组分的洗涤作用，未结合的标记结合材料将从复合层分离。因此，未结合的标签结合材料不会影响分析。

2. 发明授权

US5776710A Assay of blood or other biologic samples for target analytes 失效
标题翻译：测定目标分析物的血液或其他生物样品
公开(公告)号：US5776710A
公开(公告)日：1998-07-07
申请号：US771507
申请日：1996-12-23
申请人： Robert A. Levine , Stephen C. Wardlaw , Leon W. M. M. Terstappen , Kristen L. Manion , Rodolfo R. Rodriguez , Adrien P. Malick , Subhash Dhanesar , Stephen J. Lovell , Alvydas J. Ozinskas
发明人： Robert A. Levine , Stephen C. Wardlaw , Leon W. M. M. Terstappen , Kristen L. Manion , Rodolfo R. Rodriguez , Adrien P. Malick , Subhash Dhanesar , Stephen J. Lovell , Alvydas J. Ozinskas
IPC分类号： G01N33/543 , B01L3/14 , G01N33/49 , G01N33/537 , G01N33/569 , G01N33/58 , G01N33/558
CPC分类号： B01L3/5021 , G01N33/491 , G01N33/5375 , G01N33/56972 , G01N33/585 , Y10S435/81 , Y10S435/967 , Y10S435/971 , Y10S435/973 , Y10S436/805 , Y10S436/81 , Y10S436/824 , Y10S436/829 , Y10T436/111666
摘要： A patient's health may be diagnosed by centrifuging blood samples in a transparent tube, which tube contains one or more bodies or groups of bodies such as floats, inserts, liposomes, or plastic beads of different densities. Each density-defined body carries analyte-capture binding materials such as antigens or antibodies, which are specific to an epitope, or other specific high affinity binding site on a target analyte which target analyte may be in the blood or other sample being tested; and the level of which analyte is indicative of the patient's health. At least one labeled binding material which is also specific to an epitope, or other specific high affinity binding site on the target analyte is added to the sample so as to form labeled binding material/analyte/body complexes in the sample. Upon centrifugation, the complexes will settle out in different areas in the tube according to the respective density of the body or bodies; and the degree of label emission of the complex layers can enable qualitative and/or quantitative analyses of the sample to be made. Unbound labeled binding materials will be separated from the complexed layers by the washing action of ascending or descending components of the sample during the centrifugation step. Unbound labeled binding material will thus not interfere with the analysis.
摘要翻译：可以通过将透明管中的血液样品离心来诊断患者的健康，该管包含一个或多个不同密度的浮体，插入物，脂质体或塑料珠的主体或组。每个密度定义的身体携带分析物 - 捕获结合材料，例如抗原或抗体，其对靶分析物是特异性的，或靶分析物上的其它特异性高亲和力结合位点，其目标分析物可能在待测试的血液或其他样品中; 并且其分析物的水平表示患者的健康。将至少一种对靶分析物上的表位或其他特异性高亲和力结合位点特异性的标记结合物质加入到样品中，以便在样品中形成标记的结合材料/分析物/身体复合物。离心后，复合物将根据身体或身体的相应密度沉淀在管中的不同区域; 并且复合层的标签发射程度可以使得要进行样品的定性和/或定量分析。通过在离心步骤期间样品的上升或下降组分的洗涤作用，未结合的标记结合材料将从复合层分离。因此，未结合的标签结合材料不会影响分析。

3. 发明授权

US5834217A Assay of blood or other biologic samples for target analytes 失效
公开(公告)号：US5834217A
公开(公告)日：1998-11-10
申请号：US763858
申请日：1996-12-11
申请人： Robert A. Levine , Stephen C. Wardlaw , Leon W. M. M. Terstappen , Kristen L. Manion , Rodolfo R. Rodriguez , Adrien P. Malick , Subhash Dhanesar , Stephen J. Lovell , Alvydas J. Ozinskas
发明人： Robert A. Levine , Stephen C. Wardlaw , Leon W. M. M. Terstappen , Kristen L. Manion , Rodolfo R. Rodriguez , Adrien P. Malick , Subhash Dhanesar , Stephen J. Lovell , Alvydas J. Ozinskas
IPC分类号： G01N33/543 , B01L3/14 , G01N33/49 , G01N33/537 , G01N33/569 , G01N33/58 , G01N33/546
CPC分类号： B01L3/5021 , G01N33/491 , G01N33/5375 , G01N33/56972 , G01N33/585 , Y10S435/81 , Y10S435/967 , Y10S435/971 , Y10S435/973 , Y10S436/805 , Y10S436/81 , Y10S436/824 , Y10S436/829 , Y10T436/111666
摘要： A patient's health may be diagnosed by centrifuging blood samples in a transparent tube, which tube contains one or more bodies or groups of bodies such as floats, inserts, liposomes, or plastic beads of different densities. Each density-defined body carries analyte-capture binding materials such as antigens or antibodies, which are specific to an epitope, or other specific high affinity binding site on a target analyte which target analyte may be in the blood or other sample being tested; and the level of which analyte is indicative of the patient's health. At least one labeled binding material which is also specific to an epitope, or other specific high affinity binding site on the target analyte is added to the sample so as to form labeled binding material/analyte/body complexes in the sample. Upon centrifugation, the complexes will settle out in different areas in the tube according to the respective density of the body or bodies; and the degree of label emission of the complex layers can enable qualitative and/or quantitative analyses of the sample to be made. Unbound labeled binding materials will be separated from the complexed layers by the washing action of ascending or descending components of the sample during the centrifugation step. Unbound labeled binding material will thus not interfere with the analysis.

4. 发明授权

US4774965A Material layer volume determination with correction band 失效
标题翻译：材料层体积确定与校正带
公开(公告)号：US4774965A
公开(公告)日：1988-10-04
申请号：US68572
申请日：1987-07-01
申请人： Rodolfo R. Rodriguez , Matthew W. Lesnieskl , Charles F. Galanaugh , Robert A. Levine , Stephen C. Wardlaw , Theodore Juraschek
发明人： Rodolfo R. Rodriguez , Matthew W. Lesnieskl , Charles F. Galanaugh , Robert A. Levine , Stephen C. Wardlaw , Theodore Juraschek
IPC分类号： G01N9/12 , B01L3/14 , G01N9/36 , G01N15/05 , G01N33/48 , G01N33/49 , A61B5/14
CPC分类号： B01L3/50215 , G01N15/05
摘要： A centrifuge tube is used to hold a mixture of several constituents, and also contains a generally cylindrical float. The float settles, after centrifugation, into the zone occupied by the constituent whose volume is to be measured. The constituent layer will settle, after centrifugation, into the annular space between the tube bore and the outside of the float, and will be expanded axially due to the restricted volume of the annular space. The degree of expansion is dependent upon the respective sizes of the float O.D. and the tube bore ID, both of which must be closely controlled for accurate results. A known volume of a control material is placed in the tube to settle into the annular space during centrifugation in an area thereof outside of the constituent layer zone. The length of the band of the control material is measured after centrifugation and is compared to a known length which will result if the annular space is of the proper target volume. A correction factor is thus obtained and applied to all of the other constituent layers which were measured. The band thus forms a varying reference which reflects the actual volume of the annulus.

5. 发明授权

US06350613B1 Determination of white blood cell differential and reticulocyte counts 有权
标题翻译：测定白细胞差异和网织红细胞计数
公开(公告)号：US06350613B1
公开(公告)日：2002-02-26
申请号：US09252153
申请日：1999-02-18
申请人： Stephen C. Wardlaw , Robert A. Levine , Rodolfo R. Rodriguez
发明人： Stephen C. Wardlaw , Robert A. Levine , Rodolfo R. Rodriguez
IPC分类号： G01N3100
CPC分类号： G01N15/1475 , G01N33/5094 , G01N2015/0076 , G01N2015/0092 , G01N2015/1472 , G01N2015/1497 , Y10T436/10 , Y10T436/101666
摘要： Target nucleated cells, and target cells containing remnant ribosomal material, which are present in a quiescent anticoagulated whole blood sample are optically detected, enumerated, and analyzed in a sample chamber that has a varying through plane thickness due to convergent opposing sample chamber walls. At least one of the convergent walls of the chamber is transparent so that the blood sample can be observed. The chamber's varying thickness produces a first lesser thickness region in the chamber wherein individual red cells and quiescent monolayers of red cells in the sample will reside after the sample is introduced into and fills the chamber. Larger formed constituents such as white blood cells and nucleated red blood cells present in the sample will reside in greater thickness regions of the chamber, and non-nucleated red cells which reside in such greater thickness regions will agglomerate to form rouleaux. By admixing fluorescent dyes with the blood sample, target cells in the sample can be enumerated and differentiated by means of a scanning instrument which is able to measure different wave length color signals emitted from the target cells in the sample, and differentiate the target cells one from another by reason of the nature of the emitted color signals.
摘要翻译：目标有核细胞和含有剩余核糖体材料的靶细胞，其存在于静止抗凝全血样品中，由于收敛的相对的样品室壁在具有不同的平面厚度的样品室中进行光学检测，计数和分析。室的收敛壁中的至少一个是透明的，使得可以观察血液样本。室的不同厚度在腔室中产生第一较小的厚度区域，其中在将样品引入并填充室之后，样品中红细胞的各个红细胞和静止单层将驻留。存在于样品中的较大的成形组分如白细胞和有核红细胞将驻留在室的较大厚度区域中，并且驻留在这样较厚的区域中的未成核的红细胞将聚集形成rouleaux。通过将荧光染料与血液样品混合，样品中的靶细胞可以通过能够测量样品中靶细胞发射的不同波长颜色信号的扫描仪器进行枚举和分化，并将靶细胞分化为一由于发射的颜色信号的性质，从另一个。

6. 发明授权

US5759794A Assay of blood or other biologic samples for target analytes 失效
公开(公告)号：US5759794A
公开(公告)日：1998-06-02
申请号：US771506
申请日：1996-12-23
申请人： Robert A. Levine , Stephen C. Wardlaw , Rodolfo R. Rodriguez
发明人： Robert A. Levine , Stephen C. Wardlaw , Rodolfo R. Rodriguez
IPC分类号： G01N33/543 , B01L3/14 , G01N33/49 , G01N33/537 , G01N33/569 , G01N33/58 , G01N33/558
CPC分类号： B01L3/5021 , G01N33/491 , G01N33/5375 , G01N33/56972 , G01N33/585 , Y10S435/81 , Y10S435/967 , Y10S435/971 , Y10S435/973 , Y10S436/805 , Y10S436/81 , Y10S436/824 , Y10S436/829 , Y10T436/111666
摘要： A patient's health may be diagnosed by centrifuging blood samples in a transparent tube, which tube contains one or more bodies or groups of bodies such as floats, inserts, liposomes, or plastic beads of different densities. Each density-defined body carries analyte-capture binding materials such as antigens or antibodies, which are specific to an epitope, or other specific high affinity binding site on a target analyte which target analyte may be in the blood or other sample being tested; and the level of which analyte is indicative of the patient's health. At least one labeled binding material which is also specific to an epitope, or other specific high affinity binding site on the target analyte is added to the sample so as to form labeled binding material/analyte/body complexes in the sample. Upon centrifugation, the complexes will settle out in different areas in the tube according to the respective density of the body or bodies; and the degree of label emission of the complex layers can enable qualitative and/or quantitative analyses of the sample to be made. Unbound labeled binding materials will be separated from the complexed layers by the washing action of ascending or descending components of the sample during the centrifugation step. Unbound labeled binding material will thus not interfere with the analysis.

7. 发明授权

US4823624A Material layer volume determination with correction band 失效
标题翻译：材料层体积确定与校正带
公开(公告)号：US4823624A
公开(公告)日：1989-04-25
申请号：US248632
申请日：1988-09-26
申请人： Rodolfo R. Rodriguez , Matthew W. Lesniesky , Charles F. Galanaugh , Robert A. Levine , Stephen C. Wardlaw , Theodore Juraschek
发明人： Rodolfo R. Rodriguez , Matthew W. Lesniesky , Charles F. Galanaugh , Robert A. Levine , Stephen C. Wardlaw , Theodore Juraschek
IPC分类号： B01L3/14 , G01N15/05 , A61B5/14
CPC分类号： B01L3/50215 , G01N15/05
摘要： A centrifuge tube is used to hold a mixture of several constituents, and also contains a generally cylindrical float. The float settles, after centrifugation, into the zone occupied by the constituent whose volume is to be measured. The constituent layer will settle, after centrifugation, into the annular space between the tube bore and the outside of the float, and will be expanded axially due to the restricted volume of the annular space. The degree of expansion is dependent upon the respective sizes of the float O.D. and the tube bore ID, both of which must be closely controlled for accurate results. A known volume of a control material is placed in the tube to settle into the annular space during centrifugation in an area thereof outside of the constituent layer zone. The length of the band of the control material is measured after centrifugation and is compared to a known length which will result if the annular space is of the proper target volume. A correction factor is thus obtained and applied to all of the other constituent layers which were measured. The band thus forms a varying reference which reflects the actual volume of the annulus.
摘要翻译：离心管用于容纳几种成分的混合物，并且还包含一般为圆柱形的浮子。离心后，浮子沉降到被测量体积所占据的区域内。构成层在离心后将沉淀到管孔和浮体外部之间的环形空间中，并且由于环形空间的体积受限制而将轴向膨胀。膨胀程度取决于浮子O.D的各自尺寸。和管孔ID，两者都必须严格控制以获得准确的结果。将已知体积的对照材料放置在管中，以便在构成层区域外的区域中离心期间沉降到环形空间中。在离心后测量对照材料带的长度，并将其与已知的长度进行比较，如果环形空间具有适当的目标体积，则该长度将产生。因此获得校正因子并将其应用于所测量的所有其它构成层。因此，带形成变化的参考，其反映环的实际体积。

8. 发明授权

US4940668A Method for increasing agglutination of groups of cells to produce improved cell layer interface in centrifuged blood sample 失效
标题翻译：增加细胞组凝集以在离心血液样品中产生改善的细胞层界面的方法
公开(公告)号：US4940668A
公开(公告)日：1990-07-10
申请号：US98370
申请日：1987-09-18
申请人： Stephen C. Wardlaw , Robert A. Levine , Rodolfo R. Rodriguez , Michael R. Loken
发明人： Stephen C. Wardlaw , Robert A. Levine , Rodolfo R. Rodriguez , Michael R. Loken
IPC分类号： G01N33/49 , G01N33/50 , G01N33/80
CPC分类号： G01N33/80 , G01N33/491 , G01N33/5002 , Y10T436/107497 , Y10T436/25 , Y10T436/25375
摘要： In order to produce a more well defined interface between adjacent cell layers in a centrifuged sample of anticoagulated whole blood, a material which will bond one group of cells together is added to the blood sample prior to centrifugation. The bonding material must produce a high strength bond between one group of cells, but not effect the other cell types. The material is added prior to centrifugation of the blood sample.
摘要翻译：为了在离心的抗凝全血样品中产生相邻细胞层之间更加界定的界面，将离心之前将一组细胞结合在一起的物质加入到血液样品中。接合材料必须在一组电池之间产生高强度的结合，但不影响其他电池类型。在血液样品离心之前加入材料。

9. 发明授权

US4695553A Method for increasing agglutination of groups of cells to produce improved cell layer interface in centrifuged blood sample using antibodies 失效
标题翻译：增加细胞组凝集的方法，以使用抗体在离心血液样品中产生改善的细胞层界面
公开(公告)号：US4695553A
公开(公告)日：1987-09-22
申请号：US794127
申请日：1985-11-01
申请人： Stephen C. Wardlaw , Robert A. Levine , Rodolfo R. Rodriguez , Michael R. Loken
发明人： Stephen C. Wardlaw , Robert A. Levine , Rodolfo R. Rodriguez , Michael R. Loken
IPC分类号： G01N33/53 , C12Q1/00 , G01N33/48 , G01N33/49 , G01N33/50 , G01N33/80 , G01N1/18
CPC分类号： G01N33/5002 , G01N33/491 , G01N33/80 , Y10T436/25375
摘要： In order to produce a more well defined interface between adjacent cell layers in a centrifuged sample of anticoagulated whole blood, a material which will bond one group of cells together is added to the blood sample prior to centrifugation. The bonding material must produce a high strength bond between one group of cells, but not effect the other cell types. The material is added prior to centrifugation of the blood sample. An example of a bonding agent is a monoclonal antibody.
摘要翻译：为了在离心的抗凝全血样品中产生相邻细胞层之间更加界定的界面，将离心之前将一组细胞结合在一起的物质加入到血液样品中。接合材料必须在一组电池之间产生高强度的结合，但不影响其他电池类型。在血液样品离心之前加入材料。粘合剂的实例是单克隆抗体。

10. 发明授权

US09873118B2 Biologic fluid analysis cartridge with sample handling portion and analysis chamber portion 有权
公开(公告)号：US09873118B2
公开(公告)日：2018-01-23
申请号：US13341618
申请日：2011-12-30
申请人： John A. Verrant , Niten V. Lalpuria , Igor Nikonorov , Darryn W. Unfricht , Benjamin Ports , Stephen C. Wardlaw , Robert A. Levine , Robert Holt , Kyle Hukari
发明人： John A. Verrant , Niten V. Lalpuria , Igor Nikonorov , Darryn W. Unfricht , Benjamin Ports , Stephen C. Wardlaw , Robert A. Levine , Robert Holt , Kyle Hukari
IPC分类号： A61J1/06 , B01L3/00
CPC分类号： B01L3/502707 , B01L3/5027 , B01L3/50273 , B01L2200/027 , B01L2300/0861
摘要： A biological fluid analysis cartridge is provided. In certain embodiments, the cartridge includes a base plate extending between a sample handling portion and an analysis chamber portion. A handling upper panel is attached to the base plate within the sample handling portion. A collection port is at least partially formed with the handling upper panel. An initial channel and a secondary channel are formed between the handling upper panel and the base plate. The collection port and initial and secondary channels are in fluid communication with one another. A chamber upper panel is attached to the base plate within the analysis chamber portion. At least one analysis chamber is formed between the chamber upper panel and the base plate. The secondary channel and the analysis chamber are in fluid communication with one another.

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