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1. 发明授权

US08951749B2 Enhanced immunoassay sensor 有权
标题翻译：增强免疫测定传感器
公开(公告)号：US08951749B2
公开(公告)日：2015-02-10
申请号：US13003752
申请日：2009-07-10
申请人： Dennis Rylatt , Alastair Hodges
发明人： Dennis Rylatt , Alastair Hodges
IPC分类号： G01N33/53 , G01N33/543 , G01N33/537 , G01N33/538 , G01N33/539 , G01N33/541 , G01N33/558 , G01N33/58 , G01N33/66 , C12Q1/00 , C12Q1/26 , C12Q1/32 , B01L3/00
CPC分类号： G01N33/54366 , B01L3/5027 , B01L2200/0621 , B01L2200/10 , B01L2300/0645 , B01L2300/0825 , B01L2300/087 , B01L2400/0406 , B01L2400/0694 , C12Q1/001 , C12Q1/004 , C12Q1/005 , C12Q1/006 , C12Q1/26 , C12Q1/32 , G01N33/53 , G01N33/5302 , G01N33/5306 , G01N33/537 , G01N33/538 , G01N33/539 , G01N33/541 , G01N33/54393 , G01N33/558 , G01N33/581 , G01N33/66
摘要： Disclosed herein are devices for detecting the presence of a target analyte in a fluid sample. The biosensor device can comprise at least a reaction chamber and a detection chamber. The device can comprise a amplifying mechanism such that one target analyte molecule present in the fluid sample can lead to generation/activation of multiple detection agent molecules, and therefore, an amplified signal. Also disclosed are the methods of manufacturing and using such a biosensor device.
摘要翻译：本文公开了用于检测流体样品中目标分析物的存在的装置。生物传感器装置可以至少包括反应室和检测室。该装置可以包括放大机制，使得存在于流体样品中的一个目标分析物分子可以导致多个检测剂分子的产生/激活，并因此导致放大的信号。还公开了制造和使用这种生物传感器装置的方法。

2. 发明授权

US5422284A Method of performing affinity separation using immobilized flocculating agent on chromatographic support 失效
标题翻译：使用固定化絮凝剂进行色谱支持的亲和力分离的方法
公开(公告)号：US5422284A
公开(公告)日：1995-06-06
申请号：US145709
申请日：1993-10-29
申请人： Hon-Peng P. Lau
发明人： Hon-Peng P. Lau
IPC分类号： G01N33/538 , B01D15/08 , C07K1/16 , C07K1/22 , C07K14/00 , C07K14/435 , C07K16/00 , G01N30/52 , G01N30/88 , G01N33/539 , G01N33/543 , G01N33/553 , G01N33/544 , G01N33/546 , G01N33/551
CPC分类号： G01N33/553 , B01D15/3809 , G01N33/539 , G01N2030/528 , Y10S436/824
摘要： Disclosed is a primary chromatographic support containing an immobilized flocculating agent for use as a separation media for a secondary support consisting of microparticles used in affinity separation and heterogeneous immunoassays. In a specific embodiment, a flocculating agent such as polyethyleneimine is immobilized on a chromatographic resin packed in a column. The column so formed is then used to trap microparticles having an affinity ligand or binder for the ligand affixed thereon. Such microparticles are used as a solid support for the affinity reaction involved in a variety of immunoassay formats.
摘要翻译：公开了含有固定化絮凝剂的初级色谱载体，其用作由亲和力分离和异种免疫测定中使用的微粒组成的辅助载体的分离介质。在具体实施方案中，将聚乙烯亚胺等絮凝剂固定在柱中填充的色谱树脂上。然后将如此形成的柱用于捕获具有附着在其上的配体的亲和配体或粘合剂的微粒。这种微粒用作参与各种免疫测定形式的亲和力反应的固体支持物。

3. 发明授权

US5167925A Precipitation of multivalent antiligands with affinity surfactants 失效
标题翻译：用亲和表面活性剂沉淀多价抗配糖
公开(公告)号：US5167925A
公开(公告)日：1992-12-01
申请号：US861471
申请日：1992-04-01
申请人： Ruben G. Carbonell , Roberto Guzman , Peter K. Kilpatrick
发明人： Ruben G. Carbonell , Roberto Guzman , Peter K. Kilpatrick
IPC分类号： G01N33/53 , G01N33/539
CPC分类号： G01N33/53 , G01N33/539 , Y10S436/808
摘要： Surfactants bound to a ligand and dissolved in a single phase aqueous solution form a precipitate when a multivalent antiligand is added to the solution. This invention can be used in an affinity precipitation test procedure (and kit) for detecting the presence or absence of a multivalent antiligand in a sample suspected of containing the multivalent antiligand, in an affinity precipitation inhibition test procedure (and kit) for detecting the presence or absence of a target ligand in a sample suspected of containing the target ligand, and in a process for separating a multivalent antiligand from a crude material containing the multivalent antiligand.
摘要翻译：当向溶液中加入多价抗配偶药物时，与配体结合并溶解在单相水溶液中的表面活性剂形成沉淀物。本发明可用于亲和沉淀试验程序（和试剂盒），用于在亲和沉淀抑制试验程序（和试剂盒）中用于检测疑似含有多价反配体的样品中是否存在多价抗配偶体，以检测其存在或在怀疑含有靶配体的样品中不存在目标配体，以及从含有多价抗配偶体的粗制物质中分离多价抗配偶体的方法。

4. 发明授权

US5135875A Protein precipitation reagent 失效
标题翻译：蛋白降解试剂
公开(公告)号：US5135875A
公开(公告)日：1992-08-04
申请号：US567853
申请日：1990-08-15
申请人： Victoria P. Meucci , Elizabeth A. Simpson , Mariola B. Zajac
发明人： Victoria P. Meucci , Elizabeth A. Simpson , Mariola B. Zajac
IPC分类号： B01D21/01 , G01N1/28 , G01N33/531 , G01N33/539 , G01N33/542
CPC分类号： G01N33/539 , Y10S435/961 , Y10S435/962 , Y10S435/975 , Y10S436/80 , Y10S436/808 , Y10S436/825 , Y10T436/107497 , Y10T436/25125
摘要： A precipitation reagent for use in analytical systems for the determination of hydrophobic analytes in a biological test sample, particularly analytical systems employing specific binding proteins for such analytes. The precipitation reagent precipitates interfering proteins, hemoglobin, and other interfering substances from a biological test sample while, at the same, maintaining hydrophobic analytes in solution and minimizing the denaturation of specific binding proteins, such as, for example, antibodies, which may be present in an immunoassay system. The precipitation reagent comprises a zinc salt, a glycol, and a straight or branced alcohol from about 1 to 4 carbon atoms, and may optionally contain an acid. A preferred precipitation reagent comprises zinc sulfate, methanol and ethylene glycol, and is particularly useful in a fluorescent polarization immunoassay for the determination of hydrophobic analytes, especially cyclosporine.

5. 发明授权

US5019999A Immunoanalysis method for discriminating between antigen excess and antibody excess conditions 失效
标题翻译：用于区分抗原过量和抗体过量条件的免疫分析方法
公开(公告)号：US5019999A
公开(公告)日：1991-05-28
申请号：US252420
申请日：1988-09-30
申请人： Chester Swirski, Jr.
发明人： Chester Swirski, Jr.
IPC分类号： G01N33/53 , G01N33/536 , G01N33/539 , G01N33/557
CPC分类号： G01N33/557 , G01N33/539 , Y10S436/805
摘要： A method of immunoassay for analyzing a bodily fluid for the presence of an analyte relies on observing substantially the entire course of a precipitin reaction from initiation to substantial completion of the precipitation, defining a mathematical function which represents the amount of precipitation versus time and contains constants which can be adjusted so that the function matches the observed precipitation versus time curve, and fitting the observed data to the mathematical function by adjusting the constants so that the mathematical function matches the observed precipitation versus time curve as closely as possible. Values for the maximum rate of precipitation and for the time interval from initiation of the precipitation to the time of maximum rate of precipitation are calculated from the mathematical function. A reference curve is generated using standard containing known concentrations of analyte according to conventional analytical procedures. The values so calculated for the parameters of the precipitation can then be used to determine whether a given sample presents a condition of antigen (analyte) excess and must be discarded or a condition of antibody excess which can be used in conjunction with the reference curve to report a valid concentration of analyte.

6. 发明授权

US3976763A Chlorpromazine assay 失效
标题翻译：氯丙嗪测定
公开(公告)号：US3976763A
公开(公告)日：1976-08-24
申请号：US573227
申请日：1975-04-30
申请人： Sidney Spector
发明人： Sidney Spector
IPC分类号： C07K16/44 , G01N33/531 , G01N33/539 , G01N33/00 , A61K39/00
CPC分类号： G01N33/539 , C07K16/44 , G01N33/531 , Y10S436/804 , Y10S436/815 , Y10S436/823 , Y10S530/806
摘要： A sensitive radioimmunoassay for chlorpromazine is described. To prepare the chlorpromazine selective antiserum, an antigen is made comprising chlorpromazine covalently bonded to an immunogenic carrier material through a diazo containing linking group and the antigen is injected into a suitable host mammal to elicit the desired antiserum. A preferred linking group is diazophenylcarbonyl while bovine serum albumin is a preferred immunogenic carrier material.
摘要翻译：描述了氯丙嗪的敏感放射免疫测定法。为了制备氯丙嗪选择性抗血清，制备包含通过含重氮连接基团与免疫原性载体材料共价结合的氯丙嗪的抗原，并将抗原注射到合适的宿主哺乳动物中以引出所需的抗血清。优选的连接基团是二氮苯基羰基，而牛血清白蛋白是优选的免疫原性载体材料。

7. 发明公开

US20240288454A1 ASSAYS FOR DETECTING THE PRESENCE OR AMOUNT OF AN ANTI-DRUG ANTIBODY 审中-公开
公开(公告)号：US20240288454A1
公开(公告)日：2024-08-29
申请号：US18428535
申请日：2024-01-31
申请人： GENZYME CORPORATION
发明人： Ryan Grabert , Susan Richards , Valerie Theobald , Yuanxin Xu , Jad Zoghbi
IPC分类号： G01N33/94 , G01N33/53 , G01N33/539 , G01N33/68
CPC分类号： G01N33/94 , G01N33/5306 , G01N33/539 , G01N33/6854 , G01N2430/00
摘要： Methods and kits for detecting antibodies (e.g., anti-drug antibodies). Such methods and kits permit the detection of, for example, anti-drug antibodies in human body fluids, such as blood, plasma and scrum.

8. 发明申请

US20180088140A1 ASSAYS FOR DETECTING THE PRESENCE OR AMOUNT OF AN ANTI-DRUG ANTIBODY 审中-公开
公开(公告)号：US20180088140A1
公开(公告)日：2018-03-29
申请号：US15671663
申请日：2017-08-08
申请人： Genzyme Corporation
发明人： Ryan Grabert , Susan Richards , Valerie Theobald , Yuanxin Xu , Jad Zoghbi
IPC分类号： G01N33/94 , G01N33/68 , G01N33/53 , G01N33/539
CPC分类号： G01N33/94 , G01N33/5306 , G01N33/539 , G01N33/6854 , G01N2430/00
摘要： Methods and kits for detecting antibodies (e.g., anti-drug antibodies). Such methods and kits permit the detection of, for example, anti-drug antibodies in human body fluids, such as blood, plasma and serum.

9. 发明授权

US06593098B1 Genes encoding proteins involved in mitotic checkpoint control and methods of use thereof 有权
标题翻译：编码参与有丝分裂检查点控制的蛋白质的基因及其使用方法
公开(公告)号：US06593098B1
公开(公告)日：2003-07-15
申请号：US09555554
申请日：2000-06-01
申请人： Timothy Yen , Gordon Chan , Sandra Jablonski
发明人： Timothy Yen , Gordon Chan , Sandra Jablonski
IPC分类号： C12Q148
CPC分类号： G01N33/5011 , C07K14/4738 , C07K16/40 , C12N9/1205 , C12Q1/48 , G01N33/5008 , G01N33/5091 , G01N33/539 , G01N33/574 , G01N2333/9121
摘要： Novel human BUB genes and their encoded proteins are provided herein. The kinases encoded by the disclosed BUB1A and BUB1B genes play a pivotal role in mitotic checkpoint control. BUB3 is a substrate of these kinases, BUB genes and their encoded proteins provide valuable therapeutic targets for the design of anti-proliferative agents which inhibit the aberrant cellular proliferation observed in tumor cells.
摘要翻译：本文提供了新的人类BUB基因及其编码的蛋白质。由所公开的BUB1A和BUB1B基因编码的激酶在有丝分裂检查点控制中起关键作用。 BUB3是这些激酶的底物，BUB基因及其编码的蛋白为抑制肿瘤细胞中观察到的异常细胞增殖的抗增殖剂的设计提供有价值的治疗靶标。

10. 发明授权

US5661019A Trifunctional conjugates 失效
标题翻译：三功能共轭物
公开(公告)号：US5661019A
公开(公告)日：1997-08-26
申请号：US410014
申请日：1995-03-22
申请人： Chan S. Oh , James C. Sternberg
发明人： Chan S. Oh , James C. Sternberg
IPC分类号： C07K14/00 , C07K1/107 , C07K14/575 , C07K16/00 , C07K17/00 , G01N33/531 , G01N33/532 , G01N33/536 , G01N33/539 , G01N33/543 , G01N33/94 , C12N11/00 , C12N11/06 , G01N33/547
CPC分类号： G01N33/54353 , G01N33/531 , G01N33/532 , G01N33/539 , G01N33/54313 , G01N33/94 , Y10S435/964 , Y10S436/819 , Y10S530/81 , Y10S530/812 , Y10S530/816
摘要： A trifunctional conjugate is providing having three chemical moieties attached through a spacer moiety. At least two of the chemical moieties are relatively small molecules, usually less than about 7,000 Daltons in size. The spacer moiety is selected to impart certain steric properties to the conjugate. In one embodiment, the binding of a macromolecular specific binding partner to one of the chemical moieties sterically inhibits the binding of a different macromolecule to another chemical moieties. In another embodiment, the binding of a first chemical moiety to a macromolecule restricts the subsequent binding of a second tridentate member to a proximate location on the same macromolecule. The three chemical moieties are preferably a nitrophenylazido residue, a phenyl boronic acid residue, and a solid support or a label such as biotin. The spacer is preferably cysteine, lysine, glutamic acid, pyroglutamic acid, S-acetylmercaptosuccinic anhydride or .omega.-carbobenzoxylysine. The conjugate is useful in immunoassays and for targeted labeling of proteins.
摘要翻译：提供具有通过间隔基部分连接的三个化学部分的三官能共轭物。至少两个化学部分是相对小的分子，通常小于约7,000道尔顿。选择间隔基部分以赋予缀合物一定的空间特性。在一个实施方案中，大分子特异性结合配偶体与其中一个化学部分的结合空间地抑制不同大分子与另一化学部分的结合。在另一个实施方案中，第一化学部分与大分子的结合限制了第二三齿构件随后与相同大分子上的邻近位置的结合。三个化学部分优选为硝基苯基叠氮基残基，苯基硼酸残基和固体支持物或诸如生物素的标记。间隔物优选为半胱氨酸，赖氨酸，谷氨酸，焦谷氨酸，S-乙酰巯基琥珀酸酐或ω-羧基苯甲氧基赖氨酸。该缀合物可用于免疫测定和蛋白质靶向标记。

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