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91. 发明授权

US07560262B2 Polynucleotide sequences encoding alkaline α-galactosidases and methods of using same 失效
标题翻译：编码碱性α-半乳糖苷酶的多核苷酸序列及其使用方法
公开(公告)号：US07560262B2
公开(公告)日：2009-07-14
申请号：US10990156
申请日：2004-11-15
申请人： Arthur Schaffer , Nir Carmi , Marina Petreikov , David Granot , Yoram Eyal
发明人： Arthur Schaffer , Nir Carmi , Marina Petreikov , David Granot , Yoram Eyal
IPC分类号： C12N9/40 , C12Q1/68 , C12N15/00 , C12P21/06 , C07H21/04
CPC分类号： C12N9/2465 , C07H21/04
摘要： The present invention provides a method of identifying alkaline-α-galactosidases. Also provided are polynucleotide sequences encoding polypeptides having an alkaline-α-galactosidase activity, oligonucleotides and oligonucleotide analogs derived from the polynucleotide sequences, peptides and peptide analogues, antibodies recognizing same and methods of using same.
摘要翻译：本发明提供了鉴定碱性α-半乳糖苷酶的方法。还提供了编码具有碱性α-半乳糖苷酶活性的多肽的多核苷酸序列，衍生自多核苷酸序列的寡核苷酸和寡核苷酸类似物，肽和肽类似物，识别它们的抗体及其使用方法。

92. 发明授权

US07514254B2 Plant-derived alkaline alpha-galactosidase 失效
标题翻译：植物来源的碱性α-半乳糖苷酶
公开(公告)号：US07514254B2
公开(公告)日：2009-04-07
申请号：US11500431
申请日：2006-08-08
申请人： Arthur A. Schaffer , Gao Zhifang
发明人： Arthur A. Schaffer , Gao Zhifang
IPC分类号： C07G17/00 , A61L9/01 , A61K38/43 , A23C9/12 , A23G1/02 , C12N9/22 , C12N9/40
CPC分类号： C12N9/2465 , A23C11/106 , A23L11/33 , A23L29/06 , C12Y302/01022
摘要： There are provided novel plant-derived enzymes for hydrolysis of sugars and particularly an alkaline alpha-galactosidase which hydrolyzes a broad spectrum of galactosyl-saccharides such as melibiose, raffinose and stachyose and guar gum, at neutral to alkaline pH conditions.
摘要翻译：在中性至碱性pH条件下，提供用于水解糖的特别是碱性α-半乳糖苷酶的新型植物衍生酶，其水解广谱的半乳糖基糖如蜜二糖，棉子糖和水苏糖和瓜尔胶。

93. 发明申请

US20080274155A1 Chimeric Cannulae Proteins, Nucleic Acids Encoding Them And Methods For Making And Using Them 审中-公开
标题翻译：嵌合插管蛋白，编码它们的核酸和制造和使用它们的方法
公开(公告)号：US20080274155A1
公开(公告)日：2008-11-06
申请号：US10593846
申请日：2005-03-24
申请人： Nelson R. Barton , Eileen O'Donoghue , Ryan Short , Gerhard Frey , David Weiner , Dan E. Robertson , Steven Briggs , Paul Zorner
发明人： Nelson R. Barton , Eileen O'Donoghue , Ryan Short , Gerhard Frey , David Weiner , Dan E. Robertson , Steven Briggs , Paul Zorner
IPC分类号： A61F2/00 , C07K14/195 , C12N9/00 , C12N9/40 , C12N9/10 , C07H21/04 , C12N15/63 , C12N1/21 , C12N5/10 , C12N1/19 , C12N1/15 , A01K67/027 , A01H5/00 , C12P1/00 , A61K38/16 , A01K1/015 , B32B9/04 , A61K39/00 , A61K39/395 , A61K31/70 , C12N5/06 , A61K35/12
CPC分类号： C07K14/195 , C07K2319/00 , Y10T428/249921
摘要： The invention provides chimeric cannulae polypeptides and nanotubules and methods for making and using them. In one aspect, the invention provides compositions and methods for the identification, separation or synthesis of proteins or ligands. In one aspect, the invention provides compositions and methods for making and using nanotubules. In one aspect, the invention provides compositions and methods for the selection and purification of chiral compositions from racemic mixtures. In one aspect, the chimeric proteins and polymers (e.g., nanotubules, tubules, bundles, balls, fibers, filaments, sheets, threads, textiles) of the invention comprise a detectable moiety, e.g., a fluorescent protein. In one aspect, the invention provides a flame retardant or heat resistant device comprising a sheeting, a covering, a coating or an adhesive comprising a chimeric protein of the invention.
摘要翻译：本发明提供了嵌合插管多肽和纳米管，以及制备和使用它们的方法。一方面，本发明提供用于鉴定，分离或合成蛋白质或配体的组合物和方法。一方面，本发明提供了制备和使用纳米管的组合物和方法。一方面，本发明提供了用于从外消旋混合物中选择和纯化手性组合物的组合物和方法。在一个方面，本发明的嵌合蛋白质和聚合物（例如纳米管，小管，束，球，纤维，丝，片，丝，纺织品）包含可检测部分，例如荧光蛋白。一方面，本发明提供一种阻燃或耐热装置，其包含片剂，覆盖物，包衣或包含本发明的嵌合蛋白质的粘合剂。

94. 发明授权

US07329524B2 Agarase and gene thereof 失效
标题翻译：琼脂糖酶及其基因
公开(公告)号：US07329524B2
公开(公告)日：2008-02-12
申请号：US10467824
申请日：2002-02-26
申请人： Jun Tomono , Hiroaki Sagawa , Ikunoshin Kato
发明人： Jun Tomono , Hiroaki Sagawa , Ikunoshin Kato
IPC分类号： C12N9/24 , C12N9/40 , C12N1/21 , C12N15/63 , C12P19/26 , C07H21/04
CPC分类号： C12Y302/01081 , C12N9/2468
摘要： Production of efficiently producing agaro-oligosaccharide; a polypeptide having an agarase activity which is usable in, for example, efficiently extracting a substance such as a nucleic acid from an agarose gel; the amino acid sequence of the polypeptide; a gene encoding the polypeptide; a process for producing the polypeptide; a process for producing agaro-oligosaccharide; and a process for extracting a substance such as a nucleic acid from an agarose gel.
摘要翻译：高效生产琼脂寡糖的生产; 具有琼脂糖酶活性的多肽，其可用于例如从琼脂糖凝胶中有效地提取诸如核酸的物质; 多肽的氨基酸序列; 编码该多肽的基因; 一种生产该多肽的方法; 制备琼脂寡糖的方法; 以及从琼脂糖凝胶中提取核酸等物质的方法。

95. 发明申请

US20050221364A1 Enzymes having alpha-galactosidase activity and methods of use thereof 审中-公开
标题翻译：具有α-半乳糖苷酶活性的酶及其使用方法
公开(公告)号：US20050221364A1
公开(公告)日：2005-10-06
申请号：US11097002
申请日：2005-04-01
申请人： Dennis Murphy , John Reid , Dan Robertson
发明人： Dennis Murphy , John Reid , Dan Robertson
IPC分类号： C12N9/40 , C12Q1/68 , C07H21/04 , C12N15/09
CPC分类号： C12N9/2465
摘要： The invention relates to α-galactosidase and to polynucleotides encoding the α-galactosidase. In addition methods of designing new α-galactosidases and method of use thereof are also provided. The α-galactosidases have increased activity and stability at increased pH and temperature.
摘要翻译：本发明涉及α-半乳糖苷酶和编码α-半乳糖苷酶的多核苷酸。另外还提供了设计新的α-半乳糖苷酶的方法及其使用方法。在增加的pH和温度下，α-半乳糖苷酶具有增加的活性和稳定性。

96. 发明申请

US20050221296A1 Hepatitis-C virus type 4, 5 and 6 有权
标题翻译：丙型肝炎病毒4型，5型和6型
公开(公告)号：US20050221296A1
公开(公告)日：2005-10-06
申请号：US11090952
申请日：2005-03-25
申请人： Peter Simmonds , Peng Yap , Ian Pike
发明人： Peter Simmonds , Peng Yap , Ian Pike
IPC分类号： G01N33/53 , A61K39/00 , A61K39/29 , A61P31/12 , C07K7/08 , C07K14/18 , C07K16/10 , C07K19/00 , C12N9/10 , C12N9/40 , C12N15/09 , C12N15/51 , C12P21/08 , C12Q1/68 , C12Q1/70 , G01N33/576 , C07K14/02
CPC分类号： C07K14/005 , A61K39/00 , A61K39/12 , A61K39/29 , C12N2770/24222 , C12N2770/24234 , C12Q1/706 , C12Q1/707 , G01N33/56983 , G01N2333/18 , G01N2469/20 , Y10S436/811 , Y10S436/82 , Y10S436/825 , Y10S530/806 , Y10S530/811 , Y10S530/826
摘要： The present invention relates to a polynucleic acid composition comprising or consisting of at least one polynucleic acid containing 8 or more contiguous nucleotides corresponding to a nucleotide sequence from the region spanning positions 417 to 957 of the Core/E1 region of HCV type 3; and/or the region spanning positions 4664 to 4730 of the NS3 region of HCV type 3; and/or the region spanning positions 4892 to 5292 of the NS3/4 region of HCV type 3; and/or the region spanning positions 8023 to 8235 of the NS5 region of the BR36 subgroup of HCV type 3a; and/or the coding region of HCV type 4a starting at nucleotide 379 in the core region; and/or the coding region of HCV type 4; and/or the coding region of HCV type 5, with said nucleotide numbering being with respect to the numbering of HCV nucleic acids as shown in Table 1, and with said polynucleic acids containing at least one nucleotide difference with known HCV type 1, and/or HCV type 2 genomes in the above-indicated regions, or the complement thereof.
摘要翻译：本发明涉及包含至少一种含有8个或更多个连续核苷酸的多核苷酸的多核酸组合物，其对应于来自HCV 3型核心/ E1区域的位置417至957的区域的核苷酸序列; 和/或跨越HCV 3型NS3区域的位置4664至4730的区域; 和/或跨越HCV 3型NS3 / 4区的位置4892至5292的区域; 和/或跨越HCV3型BR36亚组的NS5区域的位置8023至8235的区域; 和/或从核心区域的核苷酸379开始的HCV型4a的编码区; 和/或HCV 4型的编码区; 和/或HCV 5型的编码区，其中所述核苷酸编号相对于表1所示的HCV核酸的编号，并且所述多核苷酸与已知的HCV I型具有至少一个核苷酸差异，和/ 或上述区域中的HCV 2型HCV基因组，或其补体。

97. 发明申请

US20050208655A1 Blood cells having modified antigenicity 审中-公开
标题翻译：具有改良抗原性的血细胞
公开(公告)号：US20050208655A1
公开(公告)日：2005-09-22
申请号：US11049271
申请日：2005-02-02
申请人： Henrik Clausen , Humberto de la Vega , Cheryl Hill , Qiyong Liu
发明人： Henrik Clausen , Humberto de la Vega , Cheryl Hill , Qiyong Liu
IPC分类号： A61K35/18 , A61P7/06 , C12N1/20 , C12N1/21 , C12N5/078 , C12N9/24 , C12N9/40 , C12Q1/34 , C12N5/08
CPC分类号： C12N5/0641 , C12N9/2402 , C12N9/2465 , C12Q1/34 , C12R1/465 , C12Y302/01022 , C12Y302/01049
摘要： This invention relates to enzymatic removal of type A and B antigens from blood group A, B, and AB reactive cells in blood products, and thereby converting these to non-A and non-B reactive cells. The invention further relates to using unique alpha N-acetylgalactosaminidases and alpha-galactosidases with superior kinetic properties for removing the immunodominant monosaccharides of the blood group A and B antigens and improved performance in enzymatic conversion of red blood cells. The preferred unique alpha-N-acetylgalactosaminidases and alpha-galactosidases exhibit the following characteristics: (i) exclusive, preferred or no less than 10% substrate specificity for the type A and B branched polysaccharide structures relative to measurable activity with simple mono- and disaccharide structures and aglycon derivatives hereof; (ii) optimal performance at neutral pH with blood group oligosaccharides and in enzymatic conversion of cells; and (iii) a favorable kinetic constant Km with mono- and oligosaccharide substrates. The conversion methods of the invention use significantly lower amounts of recombinant glycosidase enzymes than previous and result in complete sero-conversion of all blood group A and B red cells.
摘要翻译：本发明涉及从血型A，B，AB型反应性细胞中分离A型和B型抗原，从而将其转化为非A型和非B型细胞。本发明还涉及使用独特的α-N-乙酰半乳糖胺酶和具有优异动力学性质的α-半乳糖苷酶，用于去除血型A和B抗原的免疫优势单糖，并提高红细胞酶促转化的性能。优选的独特的α-N-乙酰半乳糖胺酶和α-半乳糖苷酶具有以下特征：（i）相对于简单的单糖和二糖的可测量的活性，对于A型和B型支链多糖结构的排列，优选或不低于10％的底物特异性结构和糖苷配基衍生物; （ii）在中性pH下用血型寡糖和细胞酶促转化的最佳性能; 和（iii）具有单糖和寡糖底物的有利的动力学常数Km。本发明的转化方法比以前使用显着更低量的重组糖苷酶，并导致所有血型A和B红细胞的完全血清转化。

98. 发明授权

US06936454B2 Compositions for fracturing subterranean formations 失效
$Compositions for fracturing subterranean formations$
标题翻译：压裂地层的组成
公开(公告)号：US06936454B2
公开(公告)日：2005-08-30
申请号：US10150293
申请日：2002-05-17
申请人： Robert M. Kelly , Saad A. Khan , Pascal Leduc , Akash Tayal , Robert K. Prud'homme
发明人： Robert M. Kelly , Saad A. Khan , Pascal Leduc , Akash Tayal , Robert K. Prud'homme
IPC分类号： C09K8/62 , C09K8/68 , E21B43/267 , C12N9/40
CPC分类号： E21B43/267 , C09K8/62 , C09K8/685 , Y10S507/921 , Y10S507/922 , Y10S507/923
摘要： A method of fracturing a subterranean formation which surrounds a well bore comprises the steps of providing a fracturing fluid, and injecting the fracturing fluid into the well bore at a pressure sufficient to form fractures in the subterranean formation which surrounds the well bore. The pressure is then released from the fracturing fluid, after which the fluid may be removed from the well and the well placed into production. The fracturing fluid comprises an aqueous liquid, a polysaccharide soluble or dispersible in the aqueous liquid in an amount sufficient to increase the viscosity of the aqueous liquid, an enzyme breaker which degrades said polysaccharide at a temperature above 180° F. Fracturing fluid compositions and enzyme breaker systems useful for carrying out the invention are also disclosed.
摘要翻译：压裂围绕井眼的地下地层的方法包括提供压裂流体的步骤，以及足以在围绕井眼的地层中形成裂缝的压力将压裂流体注入井眼。然后压力从压裂液中释放出来，然后可以将流体从井中移出并将井放入生产中。压裂流体包含含水液体，在水性液体中可溶或可分散的多糖，其量足以增加水性液体的粘度，该酶在大于180°F的温度下降解所述多糖。压裂液组合物和酶还公开了可用于实施本发明的断路器系统。

99. 发明授权

US06916829B2 Method for enhancing mutant enzyme activity in gaucher disease 有权
标题翻译：增强高血糖病突变酶活性的方法
公开(公告)号：US06916829B2
公开(公告)日：2005-07-12
申请号：US10304395
申请日：2002-11-26
申请人： Jian-Qiang Fan , Satoshi Ishii , Naoki Asano
发明人： Jian-Qiang Fan , Satoshi Ishii , Naoki Asano
IPC分类号： A61K31/445 , A61K31/4465 , A61K31/7008 , A61K31/7042 , A61K31/7052 , A61K31/706 , A61P43/00 , C07D207/12 , C07D211/46 , C07H17/02 , C12N9/40 , A61K31/435 , A61K31/70
CPC分类号： A61K31/445 , A61K31/70 , A61K31/7008 , C07D451/06 , C12N9/2465
摘要： Method for enhancing in a mammalian cell the activity of an enzyme associated with Gaucher Disease by administering a competitive inhibitor of glucocerebrosidase in an amount effective to enhance the activity of the enzyme. Preferred compounds for use in the method are imino sugars and related compounds. In particular, C8-12-alkyl derivatives of N-alkyl-deoxynojirimycin, isofagomine compounds, and calystegine compoiunds are effective to enhance glucocerebrosidase activity.
摘要翻译：通过以有效增加酶的活性的量施用葡糖脑苷脂酶的竞争性抑制剂，在哺乳动物细胞中增强与戈谢病相关的酶的活性的方法。用于该方法的优选化合物是亚氨基糖和相关化合物。特别地，N-烷基脱氧野尻霉素的C8-12-烷基衍生物，异佛胺化合物和钙半胱氨酸组合物对于增强葡糖脑苷脂酶活性是有效的。

100. 发明授权

US06890748B2 Production of lysosomal enzymes in plants by transient expression 失效
标题翻译：通过瞬时表达在植物中产生溶酶体酶
公开(公告)号：US06890748B2
公开(公告)日：2005-05-10
申请号：US10103327
申请日：2002-03-20
申请人： Stephen J. Garger , Thomas H. Turpen , Monto H. Kumagai
发明人： Stephen J. Garger , Thomas H. Turpen , Monto H. Kumagai
IPC分类号： C07K14/08 , C07K14/095 , C07K14/415 , C07K14/445 , C12N9/02 , C12N9/10 , C12N9/14 , C12N9/16 , C12N9/18 , C12N9/20 , C12N9/24 , C12N9/40 , C12N9/72 , C12N9/78 , C12N9/84 , C12N15/40 , C12N15/82 , C12N15/83 , C12N15/86 , C12P41/00 , C07H21/04 , C07K17/00 , C12P21/06 , C12Q1/34
CPC分类号： C12Y302/01045 , C07K14/005 , C07K14/415 , C07K14/445 , C07K2319/00 , C12N9/0059 , C12N9/0071 , C12N9/1074 , C12N9/14 , C12N9/16 , C12N9/18 , C12N9/20 , C12N9/2402 , C12N9/2465 , C12N9/6459 , C12N9/78 , C12N9/84 , C12N15/8203 , C12N15/8216 , C12N15/8242 , C12N15/8257 , C12N15/8289 , C12N15/86 , C12N2770/00022 , C12N2770/32722 , C12P41/003 , C12Y114/18001 , C12Y302/01022 , C12Y302/01031 , C12Y304/21069
摘要： The invention relates to α-galactosidase truncated at the carboxy terminus and the production of enzymatically active recombinant human and animal lysosomal enzymes involving construction and expression of recombinant expression constructs comprising coding sequences of human or animal lysosomal enzymes in a plant expression system. The plant expression system provides for post-translational modification and processing to produce a recombinant gene product exhibiting enzymatic activity. The invention is demonstrated by working examples in which transgenic tobacco plants express recombinant expression constructs comprising human glucocerebrosidase nucleotide sequences. The invention is also demonstrated by working examples in which transfected tobacco plants express recombinant viral expression constructs comprising human α galactosidase nucleotide sequences. The recombinant lysosomal enzymes produced in accordance with the invention may be used for a variety of purposes, including but not limited to enzyme replacement therapy for the therapeutic treatment of human and animal lysosomal storage diseases.
摘要翻译：本发明涉及在羧基末端截短的α-半乳糖苷酶和生产酶活性重组人和动物溶酶体酶，其涉及重组表达构建体的构建和表达，其包含在植物表达系统中编码人或动物溶酶体酶的序列。植物表达系统提供翻译后修饰和加工以产生显示酶活性的重组基因产物。本发明通过其中转基因烟草植物表达包含人葡糖脑苷脂酶核苷酸序列的重组表达构建体的实施例来证明。本发明还通过其中转染的烟草植物表达包含人α半乳糖苷酶核苷酸序列的重组病毒表达构建体的实施例来证明。根据本发明生产的重组溶酶体酶可用于多种目的，包括但不限于用于治疗性治疗人和动物溶酶体贮积病的酶替代疗法。

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