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1. 发明授权

US07560262B2 Polynucleotide sequences encoding alkaline α-galactosidases and methods of using same 失效
标题翻译：编码碱性α-半乳糖苷酶的多核苷酸序列及其使用方法
公开(公告)号：US07560262B2
公开(公告)日：2009-07-14
申请号：US10990156
申请日：2004-11-15
申请人： Arthur Schaffer , Nir Carmi , Marina Petreikov , David Granot , Yoram Eyal
发明人： Arthur Schaffer , Nir Carmi , Marina Petreikov , David Granot , Yoram Eyal
IPC分类号： C12N9/40 , C12Q1/68 , C12N15/00 , C12P21/06 , C07H21/04
CPC分类号： C12N9/2465 , C07H21/04
摘要： The present invention provides a method of identifying alkaline-α-galactosidases. Also provided are polynucleotide sequences encoding polypeptides having an alkaline-α-galactosidase activity, oligonucleotides and oligonucleotide analogs derived from the polynucleotide sequences, peptides and peptide analogues, antibodies recognizing same and methods of using same.
摘要翻译：本发明提供了鉴定碱性α-半乳糖苷酶的方法。还提供了编码具有碱性α-半乳糖苷酶活性的多肽的多核苷酸序列，衍生自多核苷酸序列的寡核苷酸和寡核苷酸类似物，肽和肽类似物，识别它们的抗体及其使用方法。

2. 发明申请

US20050208520A1 Polynucleotide sequences encoding alkaline alpha-galactosidases and methods of using same 失效
标题翻译：编码碱性α-半乳糖苷酶的多核苷酸序列及其使用方法
公开(公告)号：US20050208520A1
公开(公告)日：2005-09-22
申请号：US10990156
申请日：2004-11-15
申请人： Arthur Schaffer , Nir Carmi , Marina Petreikov , David Granot , Yoram Eyal
发明人： Arthur Schaffer , Nir Carmi , Marina Petreikov , David Granot , Yoram Eyal
IPC分类号： C07H21/04 , C12N9/40 , C12N15/09 , C12Q1/68
CPC分类号： C12N9/2465 , C07H21/04
摘要： The present invention provides a method of identifying alkaline-α-galactosidases. Also provided are novel polynucleotide sequences encoding polypeptides having an alkaline-α-galactosidase activity, oligonucleotides and oligonucleotide analogs derived from the polynucleotide sequences, peptides and peptide analogues, antibodies recognizing same and methods of using same.
摘要翻译：本发明提供了鉴定碱性α-半乳糖苷酶的方法。还提供了编码具有碱性α-半乳糖苷酶活性的多肽的新型多核苷酸序列，衍生自多核苷酸序列的寡核苷酸和寡核苷酸类似物，肽和肽类似物，识别它们的抗体及其使用方法。

3. 发明申请

US20080010704A1 Polynucleotides and polypeptides encoded therefrom and methods of using same for increasing biomass in plants and plants generated thereby 有权
标题翻译：由其编码的多核苷酸和多肽及其使用方法用于增加由此产生的植物和植物中的生物量
公开(公告)号：US20080010704A1
公开(公告)日：2008-01-10
申请号：US11822256
申请日：2007-07-03
申请人： Arthur Schaffer , Nir Dai , Marina Petreikov
发明人： Arthur Schaffer , Nir Dai , Marina Petreikov
IPC分类号： A01H3/00 , A01H5/00 , C07H21/04 , C07K14/00 , C12N5/04
CPC分类号： C12N15/8241 , C12N9/1241
摘要： A method of increasing biomass, vigor and/or yield of a plant is disclosed. The method comprises expressing within the plant an exogenous polypeptide comprising a UGGPase activity. The polypeptide may comprise an amino acid sequence at least 90% homologous, and/or at least 80% identical to SEQ ID NO: 33 as determined using the BlastP software of the National Center of Biotechnology Information (NCBI) using default parameters. Polynucleotides encoding same and plants expressing same are also disclosed.
摘要翻译：公开了增加植物的生物量，活力和/或产量的方法。该方法包括在植物内表达包含UGGPase活性的外源多肽。多肽可以包含使用国家生物技术信息中心（NCBI）的BlastP软件使用默认参数确定的与SEQ ID NO：33至少90％同源，和/或至少80％相同的氨基酸序列。还公开了编码相同的多核苷酸和表达相同的植物。

4. 发明授权

US06720485B1 Controlling starch synthesis 有权
标题翻译：控制淀粉合成
公开(公告)号：US06720485B1
公开(公告)日：2004-04-13
申请号：US09744085
申请日：2001-03-26
申请人： Arthur Schaffer , Ilan Levin , Marina Petreikov , Moshe Bar
发明人： Arthur Schaffer , Ilan Levin , Marina Petreikov , Moshe Bar
IPC分类号： A01H106
CPC分类号： C12N9/1241 , A01H1/04 , C12N15/8245
摘要： A method for controlling starch synthesis in tomatoes including providing a population of plants derived from interspecific crosses of Lycopersicon spp. with Lycopersicon esculentum genotypes, and selecting individuals of the population that each contain an allele of a gene that increases starch synthesis, the gene originating from the Lycopersicon spp.
摘要翻译：一种控制番茄淀粉合成的方法，包括提供源自番茄种间杂交的植物群体。并且选择每个含有增加淀粉合成的基因的等位基因的群体的个体，该基因来源于番茄属（Lycopersicon spp）。

5. 发明申请

US20070022504A1 Method for breeding tomatoes having reduced water content and product of the method 审中-公开
标题翻译：育种番茄含水量减少的方法
公开(公告)号：US20070022504A1
公开(公告)日：2007-01-25
申请号：US11506896
申请日：2006-08-21
申请人： Arthur Schaffer
发明人： Arthur Schaffer
IPC分类号： A01H5/00
CPC分类号： A01H5/08 , A01H1/02 , A01H1/04 , A23L19/09 , A23L27/63 , A23V2002/00
摘要： A method for breeding tomato plants that produce tomatoes with reduced fruit water content including the steps of crossing at least one Lycopersicon esculentum plant with a Lycopersicon spp. to produce hybrid seed, collecting the first generation of hybrid seeds, growing plants from the first generation of hybrid seeds, pollinating the plants of the most recent hybrid generation, collecting the seeds produced by the most recent hybrid generation, growing plants from the seeds of the most recent hybrid generation, allowing plants to remain on the vine past the point of normal ripening, and screening for reduced fruit water content as indicated by extended preservation of the ripe fruit and wrinkling of the fruit skin.
摘要翻译：一种番茄植物繁殖方法，该番茄植物产生具有降低果实水分含量的番茄，包括使至少一个番茄番茄交配番茄的步骤。收集第一代杂交种子，从第一代杂交种子种植植物，授粉最新杂交代植物的植物，收集由最近杂交代产生的种子，从种子中种植植物最新的混合动物，允许植物在正常成熟的时候保留在葡萄藤上，并筛选减少的果实水分含量，如通过延长成熟果实的保鲜和果皮的皱纹所示。

6. 发明授权

US5817913A Method for breeding tomatoes with superior taste characteristics and product of the method 失效
标题翻译：具有优良味道特征和方法产品的番茄繁殖方法
公开(公告)号：US5817913A
公开(公告)日：1998-10-06
申请号：US530216
申请日：1995-11-30
申请人： Arthur Schaffer
发明人： Arthur Schaffer
IPC分类号： A01H1/04 , A01H5/10 , A01H5/00 , A01H5/08 , C12N5/14 , C12P19/12
CPC分类号： A01H1/04 , A01H5/10 , Y10S47/01
摘要： A method for breeding tomato plants that produce tomatoes having superior taste characteristics including the steps, of crossing at least one Lycopersicon esculentum plant with a green-fruited Lycopersicon spp. from an Eriopersicon subgenus to produce hybrid seeds, collecting the hybrid (F.sub.1)seeds, growing plants from F.sub.1 seeds, pollinating the F.sub.1 plants, collecting the hybrid seeds produced by the F.sub.1 plants, growing plants from the seeds produced by the F.sub.1 plants, measuring sucrose, glucose and fructose content of ripe fruit produced from the plants grown from the seeds of the F.sub.1 plants; and selecting plants with tomato fruits having desired characteristics including a fructose/glucose ratio greater than 1.8 and fructose levels higher than 1.3% on a fresh weight basis.
摘要翻译： PCT No.PCT / US94 / 03522 Sec。 371日期：1995年11月30日 102（e）日期1995年11月30日PCT 1994年3月30日PCT公布。出版物WO94 / 22289 日期1994年10月13日一种番茄植物育种方法，该番茄植物产生具有优异味道特征的番茄，包括以下步骤：将至少一个番茄番茄植物与绿色的番茄属植物杂交。收集杂种（F1）种子，从F1种子生长植物，授粉F1植物，收集由F1植物产生的杂交种子，从F1植物生产的种子生长植物，测量从F1植物种子生长的植物产生的成熟果实的蔗糖，葡萄糖和果糖含量; 并且以鲜重为基础，选择含有果糖/葡萄糖比大于1.8且果糖含量高于1.3％的所需特征的番茄果实的植物。

7. 发明申请

US20070036883A1 Plant-derived alkaline alpha-galactosidase 失效
标题翻译：植物来源的碱性α-半乳糖苷酶
公开(公告)号：US20070036883A1
公开(公告)日：2007-02-15
申请号：US11500431
申请日：2006-08-08
申请人： Arthur Schaffer , Gao Zhifang
发明人： Arthur Schaffer , Gao Zhifang
IPC分类号： A23C9/12 , A23L1/36 , C12P21/06 , C12N9/24 , C12N9/42
CPC分类号： C12N9/2465 , A23C11/106 , A23L11/33 , A23L29/06 , C12Y302/01022
摘要： There are provided novel plant-derived enzymes for hydrolysis of sugars and particularly an alkaline alpha-galactosidase which hydrolyzes a broad spectrum of galactosyl-saccharides such as melibiose, raffinose and stachyose and guar gum, at neutral to alkaline pH conditions.
摘要翻译：在中性至碱性pH条件下，提供用于水解糖的特别是碱性α-半乳糖苷酶的新型植物衍生酶，其水解广谱的半乳糖基糖如蜜二糖，棉子糖和水苏糖和瓜尔胶。

8. 发明授权

US06607901B1 Alkaline alpha-galactosidase 失效
标题翻译：碱性α-半乳糖苷酶
公开(公告)号：US06607901B1
公开(公告)日：2003-08-19
申请号：US09744086
申请日：2001-04-25
申请人： Arthur Schaffer , Gao Zhifang
发明人： Arthur Schaffer , Gao Zhifang
IPC分类号： C12N900
CPC分类号： C12N9/2465 , A23C11/106 , A23L11/33 , A23L29/06 , C12Y302/01022
摘要： An enzyme isolated from an organism that metabolizes alpha-galactosyl containing saccharides, comprising an alpha-galactosidase (E.C. 3.2.1.22, alpha-D-galactoside galatohydrolase) with optimal activity in the neutral to alkaline pH range, and which hydrolyzes a variety of alpha-galactose containing saccharides, in particular raffinose. The enzyme is preferably a protein monomer and an ex-alpha-galactosidase.
摘要翻译：从生物体中分离的酶代谢含有α-半乳糖基的糖类的酶，其包含在中性至碱性pH范围内具有最佳活性的α-半乳糖苷酶（EC 3.2.1.22，α-D-半乳糖苷加氢水解酶），并且其水解各种α 含半乳糖的糖类，特别是棉子糖。酶优选为蛋白质单体和ex-α-半乳糖苷酶。

9. 发明授权

US07119261B1 Method for breeding tomatoes having reduced water content and product of the method 有权
标题翻译：育种番茄含水量减少的方法
公开(公告)号：US07119261B1
公开(公告)日：2006-10-10
申请号：US10069389
申请日：2000-07-04
申请人： Arthur Schaffer
发明人： Arthur Schaffer
IPC分类号： A01H5/00 , A01H1/00 , A01H1/02 , A01H5/08
CPC分类号： A01H5/08 , A01H1/02 , A01H1/04 , A01H5/10 , A23L19/09 , A23L27/63 , A23V2002/00
摘要： A method for breeding tomato plants that produce tomatoes with reduced fruit water content including the steps of crossing at least one Lycopersicon esculentum plant with a Lycopersicon spp. to produce hybrid seed, collecting the first generation of hybrid seeds, growing plants from the first generation of hybrid seeds, pollinating the plants of the most recent hybrid generation, collecting the seeds produced by the most recent hybrid generation, growing plants from the seeds of the most recent hybrid generation, allowing plants to remain on the vine past the point of normal ripening, and screening for reduced fruit water content as indicated by extended preservation of the ripe fruit and wrinkling of the fruit skin.
摘要翻译：一种番茄植物繁殖方法，该番茄植物产生具有降低果实水分含量的番茄，包括使至少一个番茄番茄交配番茄的步骤。收集第一代杂交种子，从第一代杂交种子种植植物，授粉最新杂交代植物的植物，收集由最近杂交代产生的种子，从种子中种植植物最新的混合动物，允许植物在正常成熟的时候保留在葡萄藤上，并筛选减少的果实水分含量，如通过延长成熟果实的保鲜和果皮的皱纹所示。

10. 发明申请

US20060084163A1 Plant-derived alkaline alpha-galactosidase 审中-公开
公开(公告)号：US20060084163A1
公开(公告)日：2006-04-20
申请号：US10448473
申请日：2003-05-30
申请人： Arthur Schaffer , Gao Zhifang
发明人： Arthur Schaffer , Gao Zhifang
IPC分类号： C12P19/04 , C12N9/40 , C07H21/04 , C12P21/02 , C12N5/04
CPC分类号： C12N9/2465 , A23C11/106 , A23L11/33 , A23L29/06 , C12Y302/01022
摘要： There are provided novel plant-derived enzymes for hydrolysis of sugars and particularly an alkaline alpha-galactosidase which hydrolyzes a broad spectrum of galactosyl-saccharides such as melibiose, raffinose and stachyose and guar gum, at neutral to alkaline pH conditions.

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