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    • 8. 发明申请
    • Method for the extra-corporeal qualitative and/or quantitative recording of neurotoxic substances in the blood plasma of an individual
    • 用于个体血浆中神经毒物质的额外定性和/或定量记录的方法
    • US20040211727A1
    • 2004-10-28
    • US10476440
    • 2004-06-07
    • Jan LoockSteffen R MiznerDieter G WeissJan StangaGunter W Gross
    • B01D061/42
    • G01N33/5088Y10S436/811Y10S436/82Y10S436/825
    • The invention relates to a method for the extra-corporeal qualitative and/or quantitative recording of neuro-toxic substances in the blood plasma of an individual. According to said method, a system containing neurones or neuronal networks, which have been cultivated on or applied to a micro-electrode array or a neuro-chip, in addition to a device for measuring the electrical activity of at least one or several of said neurones are provided, the neurones are brought into contact with a prepared sample of plasma and the electrical activity of the neurones is measured. The aim of the invention is to develop and improve said method in such a way that it supplies reliable, reproducible results for the recording of neuro-toxic substances in the blood plasma of an individual, in particular in the case of encephalopathic conditions, such as for example hepatic failure, or of coma conditions of various origins. To achieve this, prior to measurement, the blood plasma of an individual is prepared by being subjected to a filtration method, preferably an ultra-filtration method, whereby an exclusion limit, which causes the most extensive separation possible of the plasma protein from the other dissolved substances is selected, and/or prior to measurement, the blood plasma of an individual is prepared by being subjected to an extraction of liposoluble substances, using a solvent for liposoluble substances, and the ultra-filtrate or the extract containing the liposoluble substances, or both are used in succession or are blended together for the measurement.
    • 本发明涉及一种用于个体血浆中神经毒性物质的体外定性和/或定量记录的方法。 根据所述方法,除了用于测量所述微电极阵列或神经芯片中的至少一个或几个的电活动的装置之外,还包括已经培养在微电极阵列或神经芯片上的神经元或神经元网络的系统 提供神经元,使神经元与制备的血浆样品接触,并测量神经元的电活性。 本发明的目的是开发和改进所述方法,使得其为个体血浆中的神经毒性物质的记录提供可靠且可重现的结果,特别是在脑病条件下,例如 例如肝衰竭,或各种起因的昏迷状况。 为了实现这一点,在测量之前,通过进行过滤方法,优选超滤方法来制备个体的血浆,由此排除极限,其导致血浆蛋白质与其他蛋白质的最广泛分离 选择溶解物质,和/或测定前,通过使用脂溶性物质的溶剂,超滤液或含有脂溶性物质的提取物,对脂溶性物质进行提取,制备个体血浆, 或两者连续使用或混合在一起用于测量。