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1. 发明授权

US09273174B2 Nucleic acid detection method, and device and kit for use in same 有权
标题翻译：核酸检测方法，以及用于其中的装置和试剂盒
公开(公告)号：US09273174B2
公开(公告)日：2016-03-01
申请号：US14002886
申请日：2012-03-05
申请人： Shigehiko Miyamoto , Jun Tomono , Koji Takahashi , Sotaro Sano , Takaaki Jikihara
发明人： Shigehiko Miyamoto , Jun Tomono , Koji Takahashi , Sotaro Sano , Takaaki Jikihara
IPC分类号： C12Q1/68 , G01N21/78 , C08F222/10 , G01N21/03
CPC分类号： G01N21/78 , C08F222/10 , C12Q1/6806 , C12Q1/6846 , C12Q1/6848 , G01N21/03 , Y10T436/143333 , C12Q2547/101 , C12Q2563/103 , C12Q2563/173
摘要： The present invention aims to solve problems in the analysis of amplified nucleic acids, i.e., cost, workability, and contamination and pollution of samples. In the present invention, a sample containing a nucleic acid and a reagent for detecting the nucleic acid are mixed in a closed system. In this regard, the nucleic acid is amplified in the same closed system, and then mixed with the detecting reagent without opening the system. In order to carry out this method, for example, a device that includes the detecting reagent shielded by a coating material or the like is used.
摘要翻译：本发明旨在解决扩增核酸分析中的问题，即成本，可加工性以及样品的污染和污染。在本发明中，含有核酸和检测核酸的试剂的样品在封闭系统中混合。在这方面，核酸在相同的封闭系统中扩增，然后与检测试剂混合而不打开系统。为了实施该方法，例如，使用包含被涂料等遮蔽的检测试剂的装置。

2. 发明申请

US20130165626A1 ANTIFREEZE PROTEIN 审中-公开
标题翻译：抗体蛋白
公开(公告)号：US20130165626A1
公开(公告)日：2013-06-27
申请号：US13643811
申请日：2011-04-28
申请人： Joichi Fukuoka , Hidehisa Kawahara , Hideaki Kegasa , Naoki Arai , Jun Tomono , Shinichi Yokota
发明人： Joichi Fukuoka , Hidehisa Kawahara , Hideaki Kegasa , Naoki Arai , Jun Tomono , Shinichi Yokota
IPC分类号： C07K14/415 , C07K16/16 , A23L3/3472 , A61K8/64 , A61Q19/00 , C07K7/00 , C09K3/18
CPC分类号： A61K8/645 , A23J3/14 , A23V2002/00 , A61K2800/52 , A61Q19/00 , C09K3/185 , A23V2200/206 , A23V2250/548
摘要： An objective of the present invention is to provide an antifreeze protein which is capable of being efficiently produced on an industrial level at low cost and which is safe and has an excellent antifreezing activity for use on a practical level. Also, an objective of the present invention is to provide a polypeptide that corresponds to the active part of the antifreeze protein; a composition, a food, a biological sample protectant and a cosmetic containing the antifreeze protein or the polypeptide; and an antibody that specifically reacts with the antifreeze protein or the polypeptide. The antifreeze protein according to the present invention is characterized in being derived from a plant and having a specific amino acid sequence or being a plant seed protein.
摘要翻译：本发明的目的是提供一种能够在工业上以低成本有效地生产并且在实用水平上安全且具有优异的防冻活性的防冻蛋白。此外，本发明的目的是提供对应于抗冻蛋白的活性部分的多肽; 组合物，食品，生物样品保护剂和含有防冻蛋白或多肽的化妆品; 以及与抗冻蛋白或多肽特异性反应的抗体。根据本发明的抗冻蛋白的特征在于衍生自植物并具有特定氨基酸序列或植物种子蛋白。

3. 发明申请

US20120136138A1 ICE-CRYSTAL GROWTH INHIBITING SUBSTANCE 审中-公开
标题翻译：冰晶生长抑制物质
公开(公告)号：US20120136138A1
公开(公告)日：2012-05-31
申请号：US13377405
申请日：2010-05-27
申请人： Hideaki Kegasa , Naoki Arai , Jun Tomono , Shinichi Yokota , Hidehisa Kawahara
发明人： Hideaki Kegasa , Naoki Arai , Jun Tomono , Shinichi Yokota , Hidehisa Kawahara
IPC分类号： C07K14/415 , C07K1/34
CPC分类号： C07K14/415 , A23L3/3472 , A23L3/3526 , A23L3/36 , A23L33/18 , A61K8/645 , A61Q19/00
摘要： An objective to be achieved by the present invention is to provide an ice-crystal growth inhibiting substance which has an excellent ice-crystal growth inhibiting activity suitable for practical use and which can be produced easily, efficiently and economically in safe steps usable in food production. Another objective of the present invention is to provide a method for producing the ice-crystal growth inhibiting substance, a polypeptide which is an active part of the ice-crystal growth inhibiting substance, as well as an ice-crystal growth inhibiting substance composition, a food, a biological sample protectant and a cosmetic each containing the ice-crystal growth inhibiting substance or the polypeptide. The ice-crystal growth inhibiting substance according to the present invention is characterized in that the ice-crystal growth inhibiting substance is derived from a plant, and has a molecular weight of 400 kDa or more as measured by gel filtration chromatography.
摘要翻译：本发明要达到的目的是提供一种冰晶生长抑制物质，它具有优良的适用于实际应用的冰晶生长抑制活性，可以容易地，高效地和经济地制备用于食品生产的安全步骤。本发明的另一个目的是提供一种冰晶生长抑制物质的制造方法，作为冰晶生长抑制物质的活性成分的多肽，以及冰晶生长抑制物质组合物，食品，生物样品保护剂和含有冰晶生长抑制物质或多肽的化妆品。本发明的冰晶生长抑制物质的特征在于，冰晶生长抑制物质来自植物，通过凝胶过滤色谱法测定分子量为400kDa以上。

4. 发明申请

US20120070561A1 METHOD FOR PRODUCING HEAT PROCESSED FOOD 审中-公开
标题翻译：生产加热食品的方法
公开(公告)号：US20120070561A1
公开(公告)日：2012-03-22
申请号：US13321528
申请日：2010-05-17
申请人： Naoki Arai , Katsuaki Abe , Jun Tomono , Shinichi Yokota , Hideaki Kegasa
发明人： Naoki Arai , Katsuaki Abe , Jun Tomono , Shinichi Yokota , Hideaki Kegasa
IPC分类号： A23J3/00 , A23J1/18 , C07K14/37 , A23J1/00 , C07K14/00 , C07K14/415
CPC分类号： A23L3/37 , A23L5/00 , A23L5/15 , A23L7/00 , A23L7/196 , A23L9/00 , A23L21/00 , A23L23/00 , A23L29/281 , A23L29/30 , A23L31/00 , A23V2002/00 , A23V2200/206 , A23V2250/54
摘要： An objective to be achieved by the present invention is to provide a method for producing a heat processed food which can be uniformly heated in a short time without causing “unevenness of heating” on microwave oven cooking. Another objective of the present invention is also to provide a heat professed food, use of an antifreeze protein in producing a heat processed food, a method for improving an effect of heating treatment on a heat professed food, and an agent for improving an effect of heating treatment. The method for producing a heat processed food according to the present invention is characterized in comprising the steps of adding an antifreeze protein to a food material or a food; and cooling the resulting mixture.
摘要翻译：本发明要实现的目的是提供一种能够在短时间内均匀加热而不会在微波炉烹饪中引起“不均匀加热”的热加工食品的制造方法。本发明的另一个目的还在于提供热食品，防冻蛋白在热加工食品生产中的应用，改善对热食品的加热处理效果的方法，以及提高其效果的试剂加热处理。本发明的热加工食品的制造方法的特征在于，包括以下步骤：在食品或食品中加入防冻蛋白质; 并冷却所得混合物。

5. 发明申请

US20110117549A1 METHOD FOR DETECTING NUCLEIC ACID, AND DEVICE OR KIT 有权
标题翻译：检测核酸的方法，以及装置或试剂盒
公开(公告)号：US20110117549A1
公开(公告)日：2011-05-19
申请号：US12726680
申请日：2010-03-18
申请人： Shigehiko Miyamoto , Tomohisa Kato , Koji Takahashi , Jun Tomono
发明人： Shigehiko Miyamoto , Tomohisa Kato , Koji Takahashi , Jun Tomono
IPC分类号： G01N33/52 , C12M1/34
CPC分类号： G01N21/78 , C12Q1/6816 , G01N33/52 , G01N33/5308 , C12Q2563/173 , C12Q2537/101 , C12Q2563/137
摘要： The present invention has its object to provide a method and a device or kit for detecting a nucleic acid, which enable simple and precise visual detection of a nucleic acid amplified by a nucleic acid amplification method, without necessity of a special device.The present invention relates to a method for detecting a nucleic acid in a sample, comprising the steps of: (1) contacting a sample with a dye to react with each other; and (2) observing a substance produced by the reaction with visible light, and evaluating the presence of a nucleic acid by eye. The present invention also relates to a device or kit for detecting a nucleic acid in a sample, comprising: a carrier (a) that holds a dye which can bind to a nucleic acid; a path (c) for passing a sample through (a) the carrier; and an evaluation part (d) for observing a substance produced by the reaction between the sample and the dye with visible light, and evaluating the presence of a nucleic acid by eye.
摘要翻译：本发明的目的是提供用于检测核酸的方法和装置或试剂盒，其能够简单且精确地通过核酸扩增方法扩增的核酸的视觉检测，而不需要特殊的装置。本发明涉及一种用于检测样品中核酸的方法，包括以下步骤：（1）使样品与染料接触以进行反应; 和（2）观察通过可见光反应产生的物质，并通过眼睛评估核酸的存在。本发明还涉及用于检测样品中的核酸的装置或试剂盒，其包含：载体（a），其保持可与核酸结合的染料; 用于使样品通过（a）载体的路径（c）; 以及用可见光观察由样品和染料之间的反应产生的物质的评价部分（d），并且通过眼睛评估核酸的存在。

6. 发明授权

US07604962B2 α-agarase and process for producing the same 失效
标题翻译： α-琼脂糖酶及其制备方法
公开(公告)号：US07604962B2
公开(公告)日：2009-10-20
申请号：US12027738
申请日：2008-02-07
申请人： Jun Tomono , Yoshiko Nomura , Hiroaki Sagawa , Takeshi Sakai , Ikunoshin Kato
发明人： Jun Tomono , Yoshiko Nomura , Hiroaki Sagawa , Takeshi Sakai , Ikunoshin Kato
IPC分类号： C12P19/44 , C12N9/24
CPC分类号： C12Y302/01158 , C12N9/2465 , C12P19/04 , C12P19/24
摘要： A polypeptide having an α-agarase activity, a gene encoding the polypeptide, a method for producing the polypeptide by genetic engineering and a method for producing an agarooligosaccharide using the polypeptide.
摘要翻译：具有α-琼脂糖酶活性的多肽，编码该多肽的基因，通过遗传工程生产该多肽的方法以及使用该多肽制备琼脂低聚糖的方法。

7. 发明申请

US20080206811A1 Process For Producing Polypeptide 审中-公开
标题翻译：生产多肽的方法
公开(公告)号：US20080206811A1
公开(公告)日：2008-08-28
申请号：US11597318
申请日：2005-08-19
申请人： Toshihiro Shodai , Hiroshi Kobori , Takehiro Sagara , Hiroshi Endo , Hikaru Takakura , Jun Tomono , Hiroaki Sagawa , Hiroyuki Mukai , Ikunoshin Kato
发明人： Toshihiro Shodai , Hiroshi Kobori , Takehiro Sagara , Hiroshi Endo , Hikaru Takakura , Jun Tomono , Hiroaki Sagawa , Hiroyuki Mukai , Ikunoshin Kato
IPC分类号： C12P21/04 , C12N15/00
CPC分类号： C12N15/70 , C12N9/1276 , C12N9/22 , C12P21/02
摘要： A process for producing a target protein at low temperature, comprising inducing expression of not only a vector having introduced therein a gene coding for the target protein but also a vector having a chaperone gene introduced therein.
摘要翻译：一种在低温下生产靶蛋白的方法，包括不仅引入其中引入了编码靶蛋白的基因的载体，还诱导其中引入伴侣伴侣基因的载体。

8. 外观设计

USD725286S1 Combined micro tube and cap 有权
标题翻译：组合微管和帽
公开(公告)号：USD725286S1
公开(公告)日：2015-03-24
申请号：US29425581
申请日：2012-06-25
申请人： Shigehiko Miyamoto , Jun Tomono , Sotaro Sano
设计人： Shigehiko Miyamoto , Jun Tomono , Sotaro Sano

9. 发明申请

US20140058043A1 NUCLEIC ACID DETECTION METHOD, AND DEVICE AND KIT FOR USE IN SAME 有权
标题翻译：核酸检测方法，以及用于其的装置和试剂盒
公开(公告)号：US20140058043A1
公开(公告)日：2014-02-27
申请号：US14002886
申请日：2012-03-05
申请人： Shigehiko Miyamoto , Jun Tomono , Koji Takahashi , Sotaro Sano , Takaaki Jikihara
发明人： Shigehiko Miyamoto , Jun Tomono , Koji Takahashi , Sotaro Sano , Takaaki Jikihara
IPC分类号： C08F222/10
CPC分类号： G01N21/78 , C08F222/10 , C12Q1/6806 , C12Q1/6846 , C12Q1/6848 , G01N21/03 , Y10T436/143333 , C12Q2547/101 , C12Q2563/103 , C12Q2563/173
摘要： The present invention aims to solve problems in the analysis of amplified nucleic acids, i.e., cost, workability, and contamination and pollution of samples. In the present invention, a sample containing a nucleic acid and a reagent for detecting the nucleic acid are mixed in a closed system. In this regard, the nucleic acid is amplified in the same closed system, and then mixed with the detecting reagent without opening the system. In order to carry out this method, for example, a device that includes the detecting reagent shielded by a coating material or the like is used.
摘要翻译：本发明旨在解决扩增核酸分析中的问题，即成本，可加工性以及样品的污染和污染。在本发明中，含有核酸和检测核酸的试剂的样品在封闭系统中混合。在这方面，核酸在相同的封闭系统中扩增，然后与检测试剂混合而不打开系统。为了实施该方法，例如，使用包含被涂料等遮蔽的检测试剂的装置。

10. 发明申请

US20090035417A1 Emulsifying Agent 有权
标题翻译：乳化剂
公开(公告)号：US20090035417A1
公开(公告)日：2009-02-05
申请号：US12087586
申请日：2007-01-18
申请人： Hidehisa Kawahara , Jun Tomono , Hiroaki Inoue , Hitoshi Obata
发明人： Hidehisa Kawahara , Jun Tomono , Hiroaki Inoue , Hitoshi Obata
IPC分类号： A23L1/035 , A23L1/28
CPC分类号： B01F17/005 , A23L29/10 , A61K8/06 , A61K8/99 , A61Q19/00 , B01F17/0021
摘要： The present invention has its object to provide a novel emulsifier which is obtainable from an edible yeast and is highly safe and shows by itself a high level of emulsifying property and high emulsion stability; a water-soluble composition containing a liposoluble substance, together with the emulsifier; and methods of producing these. The present invention includes: an emulsifier which comprises, as an active ingredient, a carbohydrate- and protein-based complex derived from a culture fluid obtained by cultivating an edible yeast; a water-soluble composition which comprises an emulsifier and a liposoluble substance; and a method of producing the emulsifier and the water-soluble composition.
摘要翻译：本发明的目的是提供一种可食用酵母获得的新型乳化剂，其安全性高，本身具有高水平的乳化性和高乳化稳定性; 与乳化剂一起含有脂溶性物质的水溶性组合物; 以及生产这些的方法。本发明包括：乳化剂，其包含作为活性成分的来自通过培养可食用酵母获得的培养液的碳水化合物和蛋白质的复合物; 包含乳化剂和脂溶性物质的水溶性组合物; 以及乳化剂和水溶性组合物的制造方法。

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