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1. 发明授权

US07060252B2 Functionalized encapsulated fluorescent nanocrystals 有权
公开(公告)号：US07060252B2
公开(公告)日：2006-06-13
申请号：US10866929
申请日：2004-06-14
申请人： Emilio Barbera-Guillem
发明人： Emilio Barbera-Guillem
IPC分类号： A61B10/00 , B32B9/04
CPC分类号： C12Q1/6816 , B82Y15/00 , C12Q1/6818 , G01N33/586 , G01N33/588 , Y10T428/2989 , Y10T428/2991 , Y10T428/2993 , C12Q2563/161 , C12Q2563/107 , C12Q2523/107
摘要： Provided are a functionalized, encapsulated fluorescent nanocrystal comprising a liposome having encapsulated therein one or more fluorescent nanocrystals; use of the functionalized, encapsulated fluorescent nanocrystals in detection systems; and a method of producing functionalized, encapsulated fluorescent nanocrystals. A method of using the functionalized encapsulated fluorescent nanocrystals having affinity molecule bound thereto comprises contacting the functionalized encapsulated fluorescent nanocrystals with a sample so that complexes are formed between the functionalized encapsulated fluorescent nanocrystals and substrate for which the affinity molecule has binding specificity, if the substrate is present; exposing the complexes in the detection system to an excitation light source, and detecting a fluorescence peak emitted from the complexes, if present.

2. 发明申请

US20030008388A1 Cell culture apparatus and methods of use 有权
公开(公告)号：US20030008388A1
公开(公告)日：2003-01-09
申请号：US10183132
申请日：2002-06-25
申请人： BioCrystal, Ltd.
发明人： Emilio Barbera-Guillem , M. Bud Nelson
IPC分类号： C12M001/12
CPC分类号： C12N13/00 , C12M23/10 , C12M23/22 , C12M23/24 , C12M23/58 , C12M25/02 , C12M47/04 , C12N15/87
摘要： Provided is a cell culture apparatus for culturing cells, and optionally, for performing magnetic separation of cells desired to be cultured. The cell culture apparatus preferably comprises a frame; at least one gas permeable membrane securedly sealed in a leak-proof sealing to a frame and an opposing surface comprising a rigid surface in forming a culture chamber therebetween; and at least one resealable aperture to allow substances to be introduced into, or withdrawn from, the culture chamber.

3. 发明授权

US6126835A Device and method for magnetic separation of biological molecules 有权
标题翻译：用于分离生物分子的装置和方法
公开(公告)号：US6126835A
公开(公告)日：2000-10-03
申请号：US240811
申请日：1999-02-01
申请人： Emilio Barbera-Guillem , Marlin O. Thurston
发明人： Emilio Barbera-Guillem , Marlin O. Thurston
IPC分类号： B01D35/06 , B03C1/00 , B03C1/28 , C12N13/00 , G01N33/543
CPC分类号： A61M1/0056 , B03C1/0332 , B03C1/288 , B03C2201/18 , B03C2201/22 , Y10S435/803 , Y10S436/824
摘要： Provided is a magnetic separation device comprising a container having one or more outer surfaces; at least one magnetic sheet; and a physical coupler that is used to detachably secure the container to the magnetic sheet. A method of using the magnetic separation device according to the present invention comprises obtaining a fluid containing a mixed population of biological molecules, from which it is desired to separate at least one subpopulation of biological molecules; mixing the fluid with a magnetic separation reagent; contacting the mixture with the fluid holding chamber of the magnetic separation device; incubating the mixture for a sufficient time to allow for complexes to form between the subpopulation of biological molecules and the magnetic separation reagent; positioning the magnetic separation device in a position that magnetically attracts the complexes towards the magnetic sheet, and thereby holds them in position in the container; and removing the remainder of the fluid from the magnetic separation device.
摘要翻译：提供一种磁分离装置，其包括具有一个或多个外表面的容器; 至少一个磁性片; 以及用于将容器可拆卸地固定到磁性片的物理耦合器。使用根据本发明的磁分离装置的方法包括获得含有生物分子的混合群体的流体，期望从其分离至少一个生物分子亚群; 将流体与磁性分离试剂混合; 使混合物与磁选机的流体保持室接触; 将混合物温育足够的时间以允许在生物分子的亚群与磁分离试剂之间形成复合物; 将磁分离装置定位在磁性吸引复合体朝向磁性片的位置，从而将它们保持在容器中的适当位置; 以及从磁分离装置中除去剩余的流体。

4. 发明授权

US06955855B2 Nanocrystals 有权
公开(公告)号：US06955855B2
公开(公告)日：2005-10-18
申请号：US10245082
申请日：2002-09-17
申请人： Imad Naasani
发明人： Imad Naasani
IPC分类号： G01N33/543 , B82B1/00 , C01B19/04 , C09K11/08 , G01N33/58 , B32B5/16
CPC分类号： B82Y15/00 , G01N33/588 , Y10T428/25 , Y10T428/2991 , Y10T428/2993 , Y10T428/2998 , Y10T428/31721
摘要： Provided herein are compositions of functionalized, fluorescent nanocrystals comprising fluorescent nanocrystals coated with an imidazole-containing compound; compositions of functionalized, fluorescent nanocrystals comprising fluorescent nanocrystals coated with an imidazole-containing compound and cross-linked with a phosphine cross-linking compound; compositions of functionalized fluorescent nanocrystals operably bound to molecular probe; a process of making functionalized, fluorescent nanocrystals; and a process of using functionalized, fluorescent nanocrystals in a detection system.

5. 发明申请

US20020155108A1 Method for ex vivo loading of antigen presenting cells with antigen, and a vaccine comprising the loaded cells 审中-公开
标题翻译：抗原呈递细胞用抗原体外负载的方法，以及包含负载细胞的疫苗
公开(公告)号：US20020155108A1
公开(公告)日：2002-10-24
申请号：US10118379
申请日：2002-04-08
申请人： BioCrystal, Ltd.
发明人： Emilio Barbera-Guillem
IPC分类号： A61K039/395 , C12N005/08
CPC分类号： A61K39/0011 , A61K2039/505 , A61K2039/5154
摘要： Provided is a method for loading dendritic cells ex vivo which utilizes immune complex-mediated uptake of a soluble antigen by dendritic cells. The method involves coculturing target cells in the presence of, but not in physical contact with, expanded dendritic cells. Added to the coculture is human antibody against the soluble antigen(s), resulting in formation of immune complexes. The immune complexes bind to, and are uptaken by, the expanded dendritic cells; and with subsequent processing of the antigen into peptides, the peptide is presented as part of a peptide-MHC complex on the dendritic cell surface. The method of the present invention allows for dendritic cell presentation by MHCI and/or MHCII complexes, depending on the nature of the one or more antigens. Additionally, disclosed is a vaccine which comprises autologous dendritic cells loaded according to the method of the present invention, and autologous, irradiated, DNP-conjugated tumor cells; and a method of vaccinating an individual comprising administering the vaccine to the individual.
摘要翻译：提供了一种离体负载树突状细胞的方法，其利用免疫复合物介导的树突状细胞对可溶性抗原的摄取。该方法涉及在扩增的树突状细胞存在但不与物理接触的情况下共培养靶细胞。加入共培养物是针对可溶性抗原的人抗体，导致形成免疫复合物。免疫复合物与扩增的树突状细胞结合并被吸收; 并且随后将抗原处理成肽，肽作为树突状细胞表面上的肽-MHC复合物的一部分存在。根据本发明的一种或多种抗原的性质，本发明的方法允许由MHCI和/或MHCII复合物呈递树突状细胞。此外，公开了一种疫苗，其包含根据本发明的方法负载的自体树突状细胞和自体照射的DNP缀合的肿瘤细胞; 以及接种个体的方法，包括向个体施用疫苗。

6. 发明授权

US6114038A Functionalized nanocrystals and their use in detection systems 有权
标题翻译：功能化纳米晶体及其在检测系统中的应用
公开(公告)号：US6114038A
公开(公告)日：2000-09-05
申请号：US372729
申请日：1999-08-11
申请人： Stephanie L. Castro , Emilio Barbera-Guillem
发明人： Stephanie L. Castro , Emilio Barbera-Guillem
IPC分类号： A61K49/00 , B05D7/00 , B32B9/00 , B32B9/04 , H01L29/04
CPC分类号： B82Y30/00 , B82Y10/00 , B82Y5/00 , Y10T428/2989 , Y10T428/2993
摘要： Provided are compositions comprising water-soluble, functionalized nanocrystals. The water-soluble functionalized nanocrystals comprise quantum dots capped with a layer of a capping compound, and further comprise, by operably linking and in a successive manner, one or more additional compounds. The one or more additional compounds is comprised of at least a diaminocarboxylic acid which is operatively linked to the capping compound, and may further comprise an amino acid, an affinity ligand, or a combination thereof. Also provided are methods of using the functionalized nanocrystals having an affinity ligand to detect the presence or absence of a target substrate in a sample. The method comprises contacting the functionalized nanocrystals with the sample so that complexes are formed between the functionalized nanocrystals and substrate, if the substrate is present; exposing the complexes in the detection system to a wavelength of light suitable for exciting the functionalized nanocrystals of the complexes to emit a luminescence peak; and detecting the emitted luminescence peak.
摘要翻译：提供了包含水溶性官能化纳米晶体的组合物。水溶性官能化纳米晶体包括用封端化合物层覆盖的量子点，并且还通过可操作地连接并以连续的方式包含一种或多种另外的化合物。一种或多种另外的化合物由至少一种与封端化合物有效连接的二氨基羧酸组成，并且还可包含氨基酸，亲和配体或其组合。还提供了使用具有亲和配体的功能化纳米晶体来检测样品中目标底物的存在或不存在的方法。该方法包括使官能化的纳米晶体与样品接触，使得如果存在底物，则在官能化的纳米晶体和衬底之间形成复合物; 将检测系统中的复合物暴露于适于激发复合物的官能化纳米晶体的光的波长以发射发光峰; 并检测发射的发光峰。

7. 发明申请

US20050191313A1 Vaccine and immunotherapy for solid nonlymphoid tumor and related immune dysregulation 审中-公开
标题翻译：疫苗和免疫治疗固体非淋巴瘤及相关免疫失调
公开(公告)号：US20050191313A1
公开(公告)日：2005-09-01
申请号：US11097873
申请日：2005-04-01
申请人： Emilio Barbera-Guillem
发明人： Emilio Barbera-Guillem
IPC分类号： A61K39/395 , C07K16/28 , A61K39/00 , A61K39/385
CPC分类号： C07K16/2896 , A61K39/395 , A61K2039/505 , A61K2300/00
摘要： Provided are vaccines, methods of making the vaccines and methods for administering the vaccines for immunotherapy of an individual bearing, or at risk for developing, solid nonlymphoid tumor. The vaccine comprises an immunotherapeutic composition and tumor-associated antigen, and may further comprise one or more of an immunomodulator or a pharmaceutically acceptable carrier. A method of immunotherapy of an individual comprises administering to the individual an amount of the vaccine effective to suppress a TH2 response, and to induce a TH1 response against solid nonlymphoid tumor, in an individual having a TH2/TH1 imbalance.
摘要翻译：提供疫苗，制备疫苗的方法和用于施用个体携带者的免疫治疗疫苗，或处于发展中的固体非淋巴瘤肿瘤的风险的方法。疫苗包含免疫治疗组合物和肿瘤相关抗原，并且还可以包含免疫调节剂或药学上可接受的载体中的一种或多种。个体的免疫治疗方法包括在具有TH2 / TH1不平衡的个体中给予个体一定量的有效抑制TH2应答的疫苗，并诱导针对固体非淋巴瘤肿瘤的TH1应答。

8. 发明申请

US20030138859A1 Assay kits and methods for immune complex-mediate activation involving shed antigens 审中-公开
标题翻译：用于免疫复合物的测定试剂盒和方法 - 涉及脱落抗原的介导活化
公开(公告)号：US20030138859A1
公开(公告)日：2003-07-24
申请号：US10289732
申请日：2002-11-07
申请人： BioCrystal, Ltd.
发明人： Emilio Barbera-Guillem , M. Bud Nelson
IPC分类号： G01N033/53 , G01N033/567
CPC分类号： G01N33/564 , C07K16/30 , G01N33/6854
摘要： Methods and assay kits are provided for assaying for a measurable biological response inducible by activation of FcnullRI-expressing cells by immune complexes comprising shed antigen and anti-shed antigen antibody of an IgG subtype capable of binding FcnullRI. An assay kit comprises shed antigen, anti-shed antigen antibody of an IgG subtype capable of binding FcnullRI, and FcnullRI-expressing cells.
摘要翻译：提供了方法和测定试剂盒，用于通过包含能够结合FcγRRI的IgG亚型的脱落抗原和抗脱落抗体的免疫复合物活化来诱导可诱导的可测量的生物学应答。测定试剂盒包括脱落抗原，能够结合FcγRRI的IgG亚型的抗脱落抗原抗体和表达FcgammaRI的细胞。

9. 发明授权

US6110380A Device and method for magnetic separation of biological molecules 失效
标题翻译：用于分离生物分子的装置和方法
公开(公告)号：US6110380A
公开(公告)日：2000-08-29
申请号：US79469
申请日：1998-05-15
申请人： Emilio Barbera-Guillem
发明人： Emilio Barbera-Guillem
IPC分类号： B01D35/06 , B03C1/00 , C12N13/00
CPC分类号： B03C1/01 , B03C1/288 , B03C2201/18 , B03C2201/26 , Y10S435/803 , Y10S436/824
摘要： Provided is a magnetic separation device comprising a container having one or more outer surfaces; at least one flexible magnetic sheet; and a non-permanent adhesive that is used to detachably secure an outer surface of the container to a flexible magnetic sheet. A method of using the magnetic separation device according to the present invention comprises obtaining a fluid containing a mixed population of biological molecules, from which it is desired to separate at least one subpopulation of biological molecules; mixing the fluid with a magnetic separation reagent; contacting the mixture with the fluid holding chamber of the magnetic separation device; incubating the mixture for a sufficient time to allow for complexes to form between the subpopulation of biological molecules and the magnetic separation reagent; positioning the magnetic separation device in a position that magnetically attracts the complexes towards the flexible magnetic sheet, and thereby holds them in position in the container; and removing the remainder of the fluid from the magnetic separation device.
摘要翻译：提供一种磁分离装置，其包括具有一个或多个外表面的容器; 至少一个柔性磁性片; 以及用于将容器的外表面可拆卸地固定到柔性磁性片的非永久性粘合剂。使用根据本发明的磁分离装置的方法包括获得含有生物分子的混合群体的流体，期望从其分离至少一个生物分子亚群; 将流体与磁性分离试剂混合; 使混合物与磁选机的流体保持室接触; 将混合物温育足够的时间以允许在生物分子的亚群与磁分离试剂之间形成复合物; 将磁分离装置定位在磁性吸引复合物朝向柔性磁性片的位置，从而将其保持在容器中的适当位置; 以及从磁分离装置中除去剩余的流体。

10. 发明申请

US20050148036A1 Method for detecting a mononuclear cell phenotype related to a pro-tumor immune response 审中-公开
标题翻译：用于检测与前肿瘤免疫应答有关的单核细胞表型的方法
公开(公告)号：US20050148036A1
公开(公告)日：2005-07-07
申请号：US10997648
申请日：2004-11-24
申请人： Emilio Barbera-Guillem , M. Nelson
发明人： Emilio Barbera-Guillem , M. Nelson
IPC分类号： G01N33/569 , C12N5/00 , G01N33/574
CPC分类号： G01N33/56972 , G01N2800/52
摘要： A method for screening an individual for a pathological condition comprising a pro-tumor immune response by assaying a clinical sample, obtained from the individual, with a plurality of affinity ligands for detecting and determining an amount of mononuclear cell phenotype. The amount of mononuclear cell phenotype determined in the clinical sample is then compared to a reference value for the mononuclear cell phenotype, wherein a difference in the amount of mononuclear cell phenotype determined from the clinical sample as compared to the reference value comprises an indicator of the presence of a pro-tumor immune response.
摘要翻译：一种用于通过用多个亲和配体测定从个体获得的临床样品检测和测定单核细胞表型的量来筛选个体的病理状况的方法，其包括前肿瘤免疫应答。然后将临床样品中确定的单核细胞表型的量与单核细胞表型的参考值进行比较，其中与参考值相比，从临床样品确定的单核细胞表型的量的差异包括存在前肿瘤免疫反应。

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