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1. 发明申请

US20040152149A1 Textiles for use in bioreactors for expansion and maintenance of cells 审中-公开
标题翻译：生物反应器用于细胞扩增和维持的纺织品
公开(公告)号：US20040152149A1
公开(公告)日：2004-08-05
申请号：US10697870
申请日：2003-10-31
发明人： Lola M. Reid , Bhupender S. Gupta , Jeffrey M. MacDonald
IPC分类号： C12N005/00 , C12M001/12
CPC分类号： C12M23/06 , A61F2/06 , C12M21/08 , C12M23/20 , C12M23/34 , C12M23/58 , C12M25/06 , C12M29/10 , C12N5/0671 , C12N2501/11 , C12N2501/12 , C12N2501/148 , C12N2533/30 , C12N2535/10
摘要： A bioreactor for three-dimensional culture of liver cells is disclosed. The device is characterized by the use of textile vasculatures. A model and method for optimizing vasculature parameters is also disclosed. Liver acinar structure and physiological parameters are mimicked by sandwiching cells in the space between the two innermost woven textile hollow fibers, and creating radial flow of media from an outer compartment, through the cell mass compartment, and to an inner compartment. The theoretical optimum hydraulic permeability for the two innermost semi-permeable membranes is determined based on physiological hepatic sinusoidal blood flow and pressures. Experimental studies using a flow rate and pressure monitoring systems in conjunction with phase-contrast velocity-encoded MRI confirm theoretical results. Novel woven vascular tubes with optimum hydraulic permeability are disclosed for culturing hepatocytes in the multi-coaxial bioreactor.
摘要翻译：公开了用于肝细胞三维培养的生物反应器。该装置的特征在于使用纺织品血管系统。还公开了优化血管系统参数的模型和方法。通过将细胞夹在两个最内层的织造中空纤维之间的空间中，并且从外隔室，通过细胞块隔室和内隔室产生介质的径向流动来模拟肝腺泡结构和生理参数。基于生理肝脏正弦血流和压力确定两个最内半透膜的理论最佳水力渗透性。使用流速和压力监测系统结合相位对比度速度编码MRI的实验研究证实了理论结果。公开了具有最佳水力渗透性的新型编织血管，用于在多同轴生物反应器中培养肝细胞。

2. 发明申请

US20040115760A1 Method and device for carrying out biochemical reactions with a high throughput 审中-公开
标题翻译：用于以高产量进行生化反应的方法和装置
公开(公告)号：US20040115760A1
公开(公告)日：2004-06-17
申请号：US10722715
申请日：2003-11-25
发明人： Thomas Metzler , Hans Schels , Rolf Reichhuber , Jochen Kluge
IPC分类号： C12P021/06 , C12M001/12
CPC分类号： C12M23/34 , B01J19/0046 , B01J19/2475 , B01J2219/00051 , B01J2219/00286 , B01J2219/00315 , B01J2219/00423 , B01J2219/00481 , B01J2219/00484 , B01J2219/00495 , B01J2219/00511 , B01J2219/00585 , B01J2219/00599 , B01J2219/0072 , B01J2219/00725 , B01L3/50255 , B01L3/50855 , C12M23/12 , C12M23/38 , C12M27/02 , C12M27/16 , C12M29/04 , C12P21/02 , C40B60/14
摘要： Device, method and kit for carrying out biochemical reactions, in particular for cell-free polypeptide biosynthesis composed of an external housing which encloses an inner housing with incorporated wells and a supply chamber, wherein the wells of the inner housing each contain a producing system during the biochemical reaction, the supply chamber contains a supply liquid during the biochemical reaction and the wells of the inner housing and the supply chamber are separated by a semipermeable membrane characterized in that the inner housing has at least two wells the lower ends of which are closed by a semipermeable membrane and the upper ends of which protrude from the supply liquid contained in the supply chamber, and is connected to means for moving and incubating the producing systems and to the supply liquid.
摘要翻译：用于进行生物化学反应的装置，方法和试剂盒，特别是用于封闭具有合并孔的内部壳体的外部壳体的无细胞多肽生物合成，其中内部壳体的孔各自包含生产系统生化反应，供应室在生化反应期间含有供应液体，并且内壳体和供应室的孔由半透膜隔开，其特征在于，内壳体具有至少两个井，其下端封闭通过半透膜，其上端从包含在供给室中的供给液体突出，并且连接到用于移动和孵育生产系统和供应液体的装置。

3. 发明申请

US20040058437A1 Materials and reactor systems having humidity and gas control 审中-公开
标题翻译：具有湿度和气体控制的材料和反应堆系统
公开(公告)号：US20040058437A1
公开(公告)日：2004-03-25
申请号：US10457049
申请日：2003-06-05
发明人： Seth T. Rodgers , A. Peter Russo , Howard B. Schreyer , Andrey J. Zarur
IPC分类号： C12M001/12
CPC分类号： C12M25/02 , C12M23/12 , C12M23/24 , C12M29/04 , C12M41/34
摘要： The present invention is directed to materials and reactor systems having humidity and/or gas control. The material may have high oxygen permeability and/or low water vapor permeability. In some cases, the material may have sufficient permeance and/or permeability to allow cell culture to occur in a chip or other reactor system using the material. In certain embodiments, the material may be positioned adjacent to or abut a reaction site within a chip or reactor; in other embodiments, the material may be positioned such that it is in fluidic communication with the reaction site. The material may also be porous and/or transparent in some cases. In one set of embodiments, the material include a polymer that is branched, and/or contains bulky side groups that allow the polymer to have a more open structure. In some cases, the material may include two or more layers. Each layer may have a desired property, which may include, for example, permeability, transparency, cytophilicity, biophilicity, hydrophilicity, or a structural feature. In some embodiments, the material may be chosen so as to promote cell growth within the chip or reactor.
摘要翻译：本发明涉及具有湿气和/或气体控制的材料和反应器系统。该材料可能具有高的透氧性和/或低水蒸气渗透性。在一些情况下，该材料可具有足够的渗透性和/或渗透性，以允许细胞培养在使用该材料的芯片或其它反应器系统中发生。在某些实施方案中，材料可以位于与芯片或反应器内的反应位置相邻或邻接的位置; 在其它实施方案中，材料可以被定位成使其与反应部位流体连通。在某些情况下，材料也可以是多孔的和/或透明的。在一组实施方案中，材料包括分支的聚合物和/或包含允许聚合物具有更开放结构的庞大的侧基。在一些情况下，材料可以包括两层或更多层。每个层可以具有期望的性质，其可以包括例如渗透性，透明度，细胞毒性，生物亲和力，亲水性或结构特征。在一些实施方案中，可以选择材料以促进芯片或反应器内的细胞生长。

4. 发明申请

US20030157709A1 Chamber with adjustable volume for cell culture and organ assist 失效
标题翻译：具有可调节体积的细胞培养和器官辅助的腔室
公开(公告)号：US20030157709A1
公开(公告)日：2003-08-21
申请号：US10325437
申请日：2002-12-19
申请人： Organogenesis, Inc.
发明人： Paul A. DiMilla , Maury D. Cosman , Rachel Halych , Lisa Romito , Chris Gemmiti , Kevin Odlum
IPC分类号： C12N005/08 , C12M001/12
CPC分类号： C12M29/10 , C12M41/44
摘要： The invention features modular chambers for culturing cells in which the volume of a chamber can be adjusted without compromising the seal or sterility of the chamber. The invention is based on the principle that the volume of a chamber formed between two plates sandwiching a compressible gasket and a substantially incompressible stop can be adjusted using a gasket that forms a fluid-tight seal between the plates at a plurality of levels of compression. The invention enables the culture of cells between substantially parallel and rigid plates in which a relatively large volume can be used to seed the cells and the holdup volume reduced for perfusion without opening or otherwise disassembling the system to compromise its liquidtightness and sterility. The new closed, modular and scalable cell-culturing chamber can be thus perfused and used to culture cells (e.g., hepatocytes) with high levels of cell function in organ (e.g., liver) assist systems, for production of cells, for production of cell-derived products, such as proteins or viruses, or for systems to treat biological liquids to remove toxins, such as ammonia, add cell-synthesized products, or both.
摘要翻译：本发明提供了用于培养细胞的模块化室，其中可以调节室的体积而不损害室的密封或不可靠性。本发明基于这样的原理，即可以使用在多个压缩水平下在板之间形成流体密封的垫圈来调节形成在夹住可压缩垫圈的两个板和基本上不可压缩的止挡件之间的腔室的体积。本发明使得能够在基本上平行的和刚性的板之间培养细胞，其中可以使用相对较大体积的细胞来种植细胞，并且减少用于灌注的保留体积，而不打开或以其他方式分解系统以损害其液体密度和不育。因此可以将新的封闭的，模块化和可扩展的细胞培养室灌注并用于培养器官（例如肝）辅助系统中具有高水平细胞功能的细胞（例如肝细胞），用于细胞的产生，用于生产细胞或衍生的产品，如蛋白质或病毒，或用于处理生物液体以除去毒素（如氨），添加细胞合成产物或两者的系统。

5. 发明申请

US20020197713A1 Apparatus and method for growing cells 有权
标题翻译：用于培养细胞的装置和方法
公开(公告)号：US20020197713A1
公开(公告)日：2002-12-26
申请号：US09885920
申请日：2001-06-22
申请人： Fibercell System, Inc.
发明人： John J.S. Cadwell
IPC分类号： C12N005/06 , C12N005/00 , C12M001/12
CPC分类号： C12M23/08 , C12M25/10 , C12M27/16
摘要： A cell-culture apparatus forms an extra-capillary space between at least one hollow fiber and an enclosed chamber. Cells are placed in the extra-capillary space to grow. A media reservoir holds a cell-culture medium. The cell-culture medium is allowed to pass through a lumen of the at least one hollow fiber and to pass nutrients through the walls of the at least one hollow fiber to the cells in the extra-capillary space. Flow through the at least one hollow fiber is produced by action of gravity when a rocking or rotating motion is imparted to the media reservoir.
摘要翻译：细胞培养装置在至少一个中空纤维和封闭室之间形成毛细管外空间。将细胞置于毛细血管外空间中以生长。介质储存器容纳细胞培养基。允许细胞培养基通过至少一个中空纤维的内腔，并将营养素通过至少一个中空纤维的壁传递到毛细血管空间内的细胞。当向介质储存器施加摇摆或旋转运动时，通过重力作用产生通过至少一个中空纤维的流动。

6. 发明申请

US20030215941A1 Assay device that analyzes the absorption, metabolism, permeability and/or toxicity of a candidate compound 审中-公开
标题翻译：分析装置，分析候选化合物的吸收，代谢，渗透性和/或毒性
公开(公告)号：US20030215941A1
公开(公告)日：2003-11-20
申请号：US10387949
申请日：2003-03-12
发明人： Stewart Campbell , Enoch Kim , Gregory L. Kirk , Emanuele Ostuni , Olivier Schueller , Rocco Casagrande , Evelyn Wang , Paul Sweetnam
IPC分类号： C12M001/12 , C12N005/08
CPC分类号： B82Y30/00 , C12M23/12 , C12M23/16 , C12M25/02 , C12M29/10 , C12M35/08 , C12N2503/00 , C12N2503/02 , G01N33/5014
摘要： This invention provides device for co-culturing at least two different cell types in a two-dimensional configuration, methods of patterning at least two different cell types in a two-dimensional co-culture configuration, and uses of these devices and methods for analyzing an effect of candidate compound on such cellular cocultures. Also provided is a transmigration and extravasation device. Assay devices for analyzing the absorption, permeability, metabolism and/or toxicity of a candidate compound by a cell are provided. A microfluidic network, which is adaptable for integration with a device for coculturing is provided.
摘要翻译：本发明提供了用于以二维配置共培养至少两种不同细胞类型的装置，在二维共培养构型中构图至少两种不同细胞类型的方法，以及这些装置和方法用于分析候选化合物对这种细胞共培养物的作用。还提供了一种迁移和外渗装置。提供了用于分析细胞的候选化合物的吸收，渗透性，代谢和/或毒性的测定装置。提供了适用于与用于共同培养的装置集成的微流控网络。

7. 发明申请

US20030203478A1 Apparatus and method for growing cells 失效
公开(公告)号：US20030203478A1
公开(公告)日：2003-10-30
申请号：US10436991
申请日：2003-05-14
申请人： Fibercell Systems, Inc.
发明人： John J.S. Cadwell
IPC分类号： C12M001/12 , C12N005/02
CPC分类号： C12M23/08 , C12M25/10 , C12M27/16
摘要： A cell-culture apparatus forms an extra-capillary space between at least one hollow fiber and an enclosed chamber. Cells are placed in the extra-capillary space to grow. A media reservoir holds a cell-culture medium. The cell-culture medium is allowed to pass through a lumen of the at least one hollow fiber and to pass nutrients through the walls of the at least one hollow fiber to the cells in the extra-capillary space. Flow through the at least one hollow fiber is produced by action of gravity when a rocking or rotating motion is imparted to the media reservoir.

8. 发明申请

US20030186405A1 Micro/nano-embossing process and useful applications thereof 审中-公开
标题翻译：微/纳米压花工艺及其有用的应用
公开(公告)号：US20030186405A1
公开(公告)日：2003-10-02
申请号：US10113233
申请日：2002-04-01
申请人： The Ohio State University Research Foundation
发明人： L. James Lee , Siyi Lai
IPC分类号： C12N011/04 , C12M001/12 , A61K009/48 , A01N063/00 , A01N065/00 , A61K038/28 , B01D039/00 , B01D071/06 , G03F007/26 , B28B001/48 , B29D019/08 , B29C033/40 , F25C001/24 , B28B007/06 , A23G009/00 , B29C033/00
CPC分类号： B29C33/3857 , A61K9/0097 , B01D67/0025 , B01D69/02 , B01D2325/021 , B01D2325/08 , B29C45/02 , B29C2045/0094 , B82Y30/00
摘要： The present invention relates to a method of producing micro and nano-porous polymeric articles with well-defined pore structures.
摘要翻译：本发明涉及一种制备具有明确定义孔结构的微孔和纳米多孔聚合物制品的方法。

9. 发明申请

US20030054545A1 Cell and tissue culture modeling device and apparatus and method of using same 失效
公开(公告)号：US20030054545A1
公开(公告)日：2003-03-20
申请号：US09957063
申请日：2001-09-19
发明人： Damir Janigro , Mark S. McAllister
IPC分类号： C12M001/12 , C12M003/06
CPC分类号： C12M29/16 , C12M25/10
摘要： A cell and tissue culture modeling device comprising a housing having an interior chamber, an inlet port in fluid communication with the internal chamber, an outlet port in fluid communication with the internal chamber, a plurality of hollow fibers disposed within the interior chamber and traversing the length of the housing between the inlet port and the outlet port. Each of the plurality of hollow fibers has an interior defining an intracapillary space and the interior chamber defines an extracapillary space unoccupied by the plurality of hollow fibers. To access the extracapillary space of the device, a portion of the housing is removable. The device can be used to conduct permeability, drug efficacy, and gene expression studies.

10. 发明申请

US20030008388A1 Cell culture apparatus and methods of use 有权
公开(公告)号：US20030008388A1
公开(公告)日：2003-01-09
申请号：US10183132
申请日：2002-06-25
申请人： BioCrystal, Ltd.
发明人： Emilio Barbera-Guillem , M. Bud Nelson
IPC分类号： C12M001/12
CPC分类号： C12N13/00 , C12M23/10 , C12M23/22 , C12M23/24 , C12M23/58 , C12M25/02 , C12M47/04 , C12N15/87
摘要： Provided is a cell culture apparatus for culturing cells, and optionally, for performing magnetic separation of cells desired to be cultured. The cell culture apparatus preferably comprises a frame; at least one gas permeable membrane securedly sealed in a leak-proof sealing to a frame and an opposing surface comprising a rigid surface in forming a culture chamber therebetween; and at least one resealable aperture to allow substances to be introduced into, or withdrawn from, the culture chamber.

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