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1. 发明公开

EP0519198A3 Immobilization of PH-sensitive dyes to solid supports 失效
标题翻译：将敏感性染料固定于固体支持物上
公开(公告)号：EP0519198A3
公开(公告)日：1993-09-08
申请号：EP92107900.0
申请日：1992-05-11
申请人： Hewlett-Packard Company
发明人： Robotti, Karla M. , Myerholtz, Carl A.
IPC分类号： C09B11/04 , G01N21/80 , G01N33/52 , C09B11/06 , C09B11/08 , C09B11/22 , C09B11/24 , G01N31/22
CPC分类号： C07D307/885 , C07D307/92 , C07D327/04 , C09B11/06 , G01N31/221
摘要： Disclosed are pH dyes containing a suitable compatible substituent which permits binding of the dye to a solid support. Also disclosed are methods for synthesizing such dyes prior to their coupling to the solid supports.

2. 发明公开

EP0793097A2 Apparatus for collecting analyte samples from capillary electrophoresis 失效
标题翻译： Vorrichtung zum Sammeln von Analytproben nach einer Kapillarelektrophorese
公开(公告)号：EP0793097A2
公开(公告)日：1997-09-03
申请号：EP96305721.1
申请日：1996-08-02
申请人： Hewlett-Packard Company
发明人： Robotti, Karla M. , Myerson, Joel
IPC分类号： G01N27/447
CPC分类号： H01J49/0418 , G01N27/44717 , G01N27/4473
摘要： An apparatus (10) for collecting samples from capillary electrophoresis (CE) for matrix-assisted laser desorption ionization (MALDI) mass spectrometry (MS) is disclosed. The apparatus has a capillary (18), a metallic support (12), a porous member (32), and a power supply (28) for applying an electrical potential between the porous member (32) and an inlet end (20) of the capillary (18) to drive the sample through the capillary during CE. The capillary (18) transmits a sample from the inlet end (20) to the exit end (38) during capillary electrophoresis. The metallic support (12) supports the porous membrane (14) such that the porous membrane (14) contacts the capillary exit end (38) during CE. The metallic support (12) (with the porous membrane (14)) is suitable for placing in a mass spectrometer (52) to act as a repeller for MALDI. The porous member (32) has at least a portion (34) thereof that is generally concentric with the capillary exit end (38) and contacts the porous membrane (14) during CE. The capillary exit end (38) and the metallic support (12) are adapted to lift the capillary exit end (38) from the porous membrane (14) and reposition it on the porous membrane (14) at a different location to deposit noncontinuously different fractions of the sample exiting the capillary (18) on the porous membrane (14). When an electrically conducting liquid is present in the porous member (32), it provides electrical communication between the capillary exit end (38) and the power supply (28).
摘要翻译：用于从毛细管电泳（CE）收集用于基质辅助激光解吸电离（MALDI）质谱（MS）的分析物样品的装置。它在CE期间具有用于分析物和流体的毛细管（18）和金属支撑件（12），在CE上安装多孔膜（14）以接触毛细管出口端（38）。该支持体适合于将多孔膜放置在质谱仪中以用作MALDI的排斥反应器。多孔构件（32）围绕毛细管出口端，并且它们一起使得它们在CE期间能够与多孔膜接触并且将出口端与膜分开以进行重新定位，使得末端在不同点与膜接触以沉积非连续不同的样品的分数。多孔构件能够在存在电解质的情况下导电，并且电源（28）在多孔构件和毛细管的入口端（20）之间施加电势，以在CE期间驱动分析物通过毛细管，当电解质提供毛细管出口和多孔构件之间的电连接。还要求的是：（i）用于分析CE和MALDI质谱的用于分析怀疑含有分析物的样品的方法; （ii）CE和MALDI质谱联用方法; 和（iii）制备用于从MALDI质谱法的CE收集级分的装置的方法。县。膜是亲水性聚合物膜，优选聚烯烃膜，特别是聚丙烯或聚乙烯。基质优选选自烟酸，2,5-二羟基苯甲酸，芥子酸，琥珀酸，甘油，α-氰基-4-羟基肉桂酸和3-羟基吡啶甲酸。

3. 发明公开

EP0519198A2 Immobilization of PH-sensitive dyes to solid supports 失效
标题翻译： Immobilisierung von pH-empfindlichen Farbstoffen a festenTrägern。
公开(公告)号：EP0519198A2
公开(公告)日：1992-12-23
申请号：EP92107900.0
申请日：1992-05-11
申请人： Hewlett-Packard Company
发明人： Robotti, Karla M. , Myerholtz, Carl A.
IPC分类号： C09B11/04 , G01N21/80 , G01N33/52 , C09B11/06 , C09B11/08 , C09B11/22 , C09B11/24 , G01N31/22
CPC分类号： C07D307/885 , C07D307/92 , C07D327/04 , C09B11/06 , G01N31/221
摘要： Disclosed are pH dyes containing a suitable compatible substituent which permits binding of the dye to a solid support. Also disclosed are methods for synthesizing such dyes prior to their coupling to the solid supports.
摘要翻译：公开了含有合适的相容取代基的pH染料，其允许染料与固体支持物结合。还公开了在与固体支持物偶联之前合成这些染料的方法。

4. 发明公开

EP0657737A3 Method and reagents for binding chemical analytes to a substrate surface, and related analytical devices and diagnostic techniques 失效
标题翻译：程序和化学到基材表面上的分析物的结合试剂，以及相关的分析装置和诊断方法。
公开(公告)号：EP0657737A3
公开(公告)日：1996-04-10
申请号：EP94308986.2
申请日：1994-12-02
申请人： Hewlett-Packard Company
发明人： Tom-Moy, May , Robotti, Karla M. , Myerson, Joel
IPC分类号： G01N33/543 , G01N27/00 , G01N33/58 , G01N33/531 , C07D495/04 , G01N33/53
CPC分类号： C07D495/04 , G01N29/022 , G01N33/54353 , G01N33/5438 , G01N2291/0255 , G01N2291/0256 , G01N2291/0423 , G01N2291/0425 , Y10S435/962 , Y10S436/807 , Y10S436/822
摘要： A method for detecting an analyte of interest in a sample includes the steps of binding the analyte to the surface of a substrate through a biotin-biotin binding protein interaction, contacting the surface-bound analyte with a quantitatively detectable analyte-binding moiety that binds thereto, measuring the quantity of detectable moiety bound to the substrate surface (11) and deriving therefrom the quantity of analyte in solution. A preferred use for the present method is in conjunction with a piezoelectric surface transverse wave device.

5. 发明公开

EP0657737A2 Method and reagents for binding chemical analytes to a substrate surface, and related analytical devices and diagnostic techniques 失效
标题翻译：程序和化学到基材表面上的分析物的结合试剂，以及相关的分析装置和诊断方法。
公开(公告)号：EP0657737A2
公开(公告)日：1995-06-14
申请号：EP94308986.2
申请日：1994-12-02
申请人： Hewlett-Packard Company
发明人： Tom-Moy, May , Robotti, Karla M. , Myerson, Joel
IPC分类号： G01N33/543 , G01N27/00 , G01N33/58 , G01N33/531 , C07D495/04 , G01N33/53
CPC分类号： C07D495/04 , G01N29/022 , G01N33/54353 , G01N33/5438 , G01N2291/0255 , G01N2291/0256 , G01N2291/0423 , G01N2291/0425 , Y10S435/962 , Y10S436/807 , Y10S436/822
摘要： A method for detecting an analyte of interest in a sample includes the steps of binding the analyte to the surface of a substrate through a biotin-biotin binding protein interaction, contacting the surface-bound analyte with a quantitatively detectable analyte-binding moiety that binds thereto, measuring the quantity of detectable moiety bound to the substrate surface (11) and deriving therefrom the quantity of analyte in solution. A preferred use for the present method is in conjunction with a piezoelectric surface transverse wave device.
摘要翻译：一种用于样品中感兴趣的分析物的检测方法包括通过生物素 - 生物素结合蛋白相互作用的分析物结合到衬底的表面上，用定量检测的分析物 - 结合部分接触的表面结合的分析物的步骤做与其结合，测量结合于基板表面（11）可检测部分的量和分析物从在溶液中的量导出那里。用于本发明方法的优选用途是在有压电表面横波设备结合。

6. 发明公开

EP0596700A1 Cis-diol detection method and solid supports 失效
标题翻译： CIS-DIOL-Nachweismethode und festeTräger。
公开(公告)号：EP0596700A1
公开(公告)日：1994-05-11
申请号：EP93308746.2
申请日：1993-11-02
申请人： Hewlett-Packard Company
发明人： Robotti, Karla M.
IPC分类号： G01N33/52 , G01N33/66 , C09B69/10 , C09B46/00 , C09B31/00
CPC分类号： G01N33/66 , C09B29/0007 , G01N31/22
摘要： The present invention is directed to methods and kits for measurement of cis-diols in a sample by means of dyes which produce a spectral change upon reaction or complexation with cis-diols. The present invention is also directed to solid supports having such dyes as substituents thereon. In particular, measurement of glucose using azo boronic dyes is disclosed.
摘要翻译：本发明涉及用于通过在与顺式二醇反应或与顺式二醇络合时产生光谱变化的染料测量样品中顺式二醇的方法和试剂盒。本发明还涉及具有这样的染料作为其上的取代基的固体支持物。特别地，公开了使用偶氮硼染料测量葡萄糖。

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