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1. 发明授权

US06610494B2 Solid-phase activity assay for biologically active substance 失效
标题翻译：生物活性物质的固相活性测定
公开(公告)号：US06610494B2
公开(公告)日：2003-08-26
申请号：US09180819
申请日：1999-05-20
申请人： Ronald R. Marquardt , Xiao Hao , Guojie Wang , Zhiqun Zhang , Zhibo Gan
发明人： Ronald R. Marquardt , Xiao Hao , Guojie Wang , Zhiqun Zhang , Zhibo Gan
IPC分类号： G01N33533
CPC分类号： C12Q1/34 , G01N33/54306 , G01N2333/924 , G01N2333/948 , G01N2400/24
摘要： A method of detecting via a solid-phase assay the amount of biological activity, identity and/or the quantity of a biologically active substance is disclosed. The method utilizes the biological activity of the substance itself to provide the method of detection. The method provides competitive and noncompetitive assays.
摘要翻译：公开了通过固相测定法检测生物活性的量，生物活性物质的身份和/或数量的方法。该方法利用物质本身的生物活性提供检测方法。该方法提供竞争性和非竞争性测定。

2. 发明授权

US06596546B1 Detection of antibodies to bacterial antigens by fluorescence polarization 有权
标题翻译：通过荧光偏振检测细菌抗原的抗体
公开(公告)号：US06596546B1
公开(公告)日：2003-07-22
申请号：US09400798
申请日：1999-09-22
申请人： Michael E. Jolley , Klaus H. Nielsen
发明人： Michael E. Jolley , Klaus H. Nielsen
IPC分类号： G01N33533
CPC分类号： G01N33/56916 , G01N33/56911 , G01N33/5695 , G01N2333/23 , G01N2333/35 , G01N2400/50 , Y02A50/56
摘要： In a homogeneous immunoassay, fluorophore-conjugated lipopolysaccharide derived bacterial antigens are reacted with antibodies specific for the antigens. Quantitative detection of the formation of an immune complex is obtained by measuring the change in fluorescence polarization after complex formation. The reaction occurs quickly (less than two minutes to equilibrium), and involves the addition of only one reagent to a diluted serum specimen. The absence of a solid phase separation step eliminates false positive results and increases throughput.
摘要翻译：在均相免疫测定中，使荧光团结合的脂多糖衍生的细菌抗原与抗原特异性抗体反应。通过测量复合物形成后荧光极化的变化，可以获得免疫复合物形成的定量检测。反应快速发生（不到两分钟至平衡），并且仅向稀释的血清样品中加入一种试剂。没有固相分离步骤消除了假阳性结果并增加了产量。

3. 发明授权

US06243980B1 Protease inhibitor assay 失效
标题翻译：蛋白酶抑制剂测定
公开(公告)号：US06243980B1
公开(公告)日：2001-06-12
申请号：US09035820
申请日：1998-03-06
申请人： Irena Bronstein , John Voyta , Michelle Palmer , Bonnie Tillotson
发明人： Irena Bronstein , John Voyta , Michelle Palmer , Bonnie Tillotson
IPC分类号： G01N33533
CPC分类号： G01N33/582 , C12Q1/37 , G01N33/581 , G01N2333/81 , Y10S436/80 , Y10S436/805
摘要： Heterogenous and homogenous assays are provided for the detection of protease inhibitory activity in a sample or target compound, taking advantage of the chemiluminescent characteristics of 1,2-dioxetanes. In the heterogenous assay, a peptide bearing a cleavage site for the protease of interest is provided with a first member of a first ligand binding pair at one end, and a first member of a second ligand binding pair at the other end. The other member of the first ligand binding pair is attached to a surface, which binds the peptide, or protease substrate, to the surface. The peptide substrate is combined with the protease and target compound or sample. Substrate cleavage, if not inhibited, is allowed to occur, and any unbound cleaved fragments are removed. An enzyme complexed with the second member of the second ligand binding pair is added, and allowed to bind to any of the (uncleaved) first member of the second ligand binding pair remaining. Unbound complex is removed, and a 1,2-dioxetane substrate for the enzyme is added. If any peptide substrate has not been cleaved, the dioxetane will chemiluminesce, indicating inhibitory activity. In a homogenous assay, the same substrate bears at one end a fluorescent energy accepting moiety, and at the other end a 1,2-dioxetane or precursor. If the substrate is cleaved by the protease, the dioxetane and the fluorescent moiety are not in close physical relationship, and no energy transfer occurs when the dioxetane is caused to decompose. If cleavage has not occurred, indicating inhibition, when the dioxetane is caused to decompose, energy is transferred to the fluorescing entity, which releases light of a wavelength recognizably distinct from that of the dioxetane.
摘要翻译：提供异源和均一的测定法，用于检测样品或目标化合物中蛋白酶抑制活性，利用1,2-二氧杂环己烷的化学发光特性。在异源测定中，具有目的蛋白酶的切割位点的肽在一端具有第一配体结合对的第一个成员，另一端具有第二配体结合对的第一个成员。第一配体结合对的另一个成员连接到表面，其将肽或蛋白酶底物结合到表面。将肽底物与蛋白酶和目标化合物或样品组合。如果不抑制底物裂解，则可以发生底物裂解，并且去除任何未结合的切割的片段。加入与第二配体结合对的第二个成员配合的酶，并使其与残留的第二配体结合对的任何（未切割的）第一个成员结合。去除未结合的络合物，并加入酶的1,2-二氧环乙烷底物。如果任何肽底物未被切割，二氧杂环丁烷将化学发光，表明抑制活性。在同质测定中，相同的底物在一端承载荧光能量接受部分，另一端在1,2-二氧杂环丁烷或前体上。如果底物被蛋白酶切割，二氧杂环丁烷和荧光部分不是很密切的物理关系，当二氧杂环丁烷分解时，不会发生能量转移。如果没有发生裂解，表明抑制，当二氧杂环丁烷分解时，能量被转移到荧光实体，其释放与二氧杂环丁烷的波长可辨别的波长的光。

4. 发明授权

US06204002B1 Gangliosides having fluorescent-tagged ceramide moieties 失效
标题翻译：神经节苷脂具有荧光标记的神经酰胺部分
公开(公告)号：US06204002B1
公开(公告)日：2001-03-20
申请号：US09091780
申请日：1998-12-23
申请人： Takao Iida , Yutaka Ohira
发明人： Takao Iida , Yutaka Ohira
IPC分类号： G01N33533
CPC分类号： C07H15/04 , G01N33/56983 , Y02P20/55 , Y10S435/975 , Y10S436/80 , Y10S436/811 , Y10S436/818
摘要： The invention provides a compound represented by the following formula (I) wherein m is an integer of 2-15; n is an integer of 0-12; R represents a sugar chain or a derivative thereof; R′ represents a general formula (II) (wherein p is an integer of 0-4, q is an integer of 0-5; r is an integer of 2-5; R12 and R13 are the same or different and each represents a hydrogen atom, a lower alkyl group, an aliphatic acyl group, a nitro group or a nitrile group), R1 represents a hydrogen atom or a protective group of carboxylic acid, R2, R4 and R5 are the same or different and each represents a hydrogen atom, an aliphatic or aromatic acyl group; R3 represents an acetyl group, a glycolyl group or a trifluoroacetyl group; useful as reagent for detecting influenza virus A.
摘要翻译：本发明提供由下式（I）表示的化合物，其中m为2-15的整数; n为0-12的整数; R代表糖链或其衍生物; R'表示通式（II）（其中p为0-4的整数，q为0-5的整数; r为2-5的整数; R12和R13相同或不同，各自表示氢原子，低级烷基，脂族酰基，硝基或腈基），R 1表示氢原子或羧酸的保护基，R 2，R 4和R 5相同或不同，各自表示氢原子，脂族或芳族酰基; R3表示乙酰基，乙酰基或三氟乙酰基; 用作检测流感病毒A的试剂。

5. 发明授权

US06190923B1 Diethylenetriamine-N,N′,N″-triacetic acid derivatives 失效
标题翻译：二亚乙基三胺-N，N'，N“ - 三乙酸衍生物
公开(公告)号：US06190923B1
公开(公告)日：2001-02-20
申请号：US09148733
申请日：1998-09-04
申请人： David K. Johnson
发明人： David K. Johnson
IPC分类号： G01N33533
CPC分类号： C07D417/12 , C07D277/46 , G01N33/84
摘要： The present invention relates to the field of immunoassays for metal ions. The invention presents: chelators, chelates, antibodies specific for the chelates, tracers comprising chelates conjugated to detectable labels, and immunoassays utilizing the foregoing.
摘要翻译：本发明涉及金属离子的免疫测定领域。本发明提供：螯合剂，螯合物，对螯合物特异的抗体，包含与可检测标记物缀合的螯合物的示踪剂，以及利用上述的免疫测定法。

6. 发明授权

US06673629B2 Neutralization of polycations in a chromatographic device for whole blood use 失效
标题翻译：用于全血使用的色谱装置中的聚阳离子中和
公开(公告)号：US06673629B2
公开(公告)日：2004-01-06
申请号：US09007651
申请日：1998-01-15
申请人： Toru Yoshimura , Toshihiro Ogasawara , Michihiro Saito , John P. Groff
发明人： Toru Yoshimura , Toshihiro Ogasawara , Michihiro Saito , John P. Groff
IPC分类号： G01N33533
CPC分类号： G01N33/56988 , G01N30/90 , G01N33/558 , G01N33/5695 , G01N33/571 , G01N33/5764 , G01N2333/162 , G01N2333/20 , G01N2333/35 , Y10S435/962 , Y10S436/824 , Y10S436/825 , Y10T436/105831 , Y10T436/106664
摘要： A chromatography assay device and method for use with whole blood samples utilizing a red blood cell separating agent to aggregate red blood cells and permit plasma or serum to flow by capillary action and a neutralizing agent to neutralize any effects the red blood cell separating agent may have on the device and method.
摘要翻译：一种色谱分析装置和方法，用于利用红细胞分离剂聚集红细胞并允许血浆或血清通过毛细作用流动的全血样品和中和剂以中和红细胞分离剂可能具有的任何作用在设备和方法上。

7. 发明授权

US06632603B1 Non-captive substrate liquid phase immunoassay 失效
标题翻译：非捕获底物液相免疫测定
公开(公告)号：US06632603B1
公开(公告)日：2003-10-14
申请号：US08914700
申请日：1997-08-19
申请人： Thomas T. Hubscher , Erik P. Lillehoj
发明人： Thomas T. Hubscher , Erik P. Lillehoj
IPC分类号： G01N33533
CPC分类号： G01N33/585 , B01L3/5023 , B01L2300/0825 , B01L2400/0406 , G01N33/538 , G01N2333/4737 , Y10S435/971 , Y10S435/975 , Y10S436/824
摘要： An immunoassay of a liquid biological specimen for a specific analyte is conducted by forming a test sample by contacting the specimen with: (1) a binding substance of a ligand, antiligand or receptor capable of binding the analyte and (2) a detector substance of a colloidal gold or silver labeled ligand or antiligand to form a test sample. The test sample is applied by flowing onto a defined zone of an insoluble porous support film having a pore size impassable to a complex formed between the analyte, if present, with the binding substance and the detector substance, but passable to the binding substance and detector substance while remaining uncomplexed in the absence of the desired analyte. If the analyte is present in the test specimen, the detector substance binds with the analyte and the binding substance to form a visually inspectable complex on the surface of the porous support film. After application of the test sample to the porous support, the surface of the porous support is visually inspected for color to determine the presence and quantity or the absence of the analyte being assayed.
摘要翻译：对特定分析物的液体生物样品进行免疫测定，通过使样品与以下物质接触来形成测试样品：（1）能够结合分析物的配体，反配体或受体的结合物质和（2）检测物质胶体金或银标记的配体或反配体以形成测试样品。通过流动到不溶性多孔载体膜的限定区域上来施加测试样品，所述不溶性多孔载体膜的孔隙尺寸不能通过分析物（如果存在）与结合物质和检测物质形成的复合物通过，但可通过结合物质和检测器物质，同时在不存在所需分析物的情况下保持未络合。如果分析物存在于测试样品中，检测器物质与分析物和结合物质结合，以在多孔载体膜的表面上形成目视检查的复合物。在将测试样品施加到多孔载体上之后，目视检查多孔载体的表面的颜色，以确定待分析物的存在和数量或不存在。

8. 发明授权

US06350579B1 Extended dynamic range assays 有权
标题翻译：扩展动态范围测定
公开(公告)号：US06350579B1
公开(公告)日：2002-02-26
申请号：US09649636
申请日：2000-08-28
申请人： Norman C. Nelson
发明人： Norman C. Nelson
IPC分类号： G01N33533
CPC分类号： G01N33/54306 , C12Q1/6816 , Y10S435/962 , Y10S435/968 , Y10S436/80 , Y10S436/814 , Y10S436/815 , Y10S436/817
摘要： A method and compositions for the detection and/or quantification of an analyte through the use of a plurality of labeled probes, with two or more said probes targeted to different regions of said analyte. In specific embodiments, the labels are separately distinguishable, and/or are present at different specific activities on the labels.
摘要翻译：一种用于通过使用多个标记探针来检测和/或定量分析物的方法和组合物，其中两个或多个所述探针靶向所述分析物的不同区域。在具体实施方案中，标签是分开区分的，和/或以不同的特定活性存在于标签上。

9. 发明授权

US06235541B1 Patterning antibodies on a surface 失效
标题翻译：在表面上形成抗体
公开(公告)号：US06235541B1
公开(公告)日：2001-05-22
申请号：US09306386
申请日：1999-05-06
申请人： Robert A. Brizzolara
发明人： Robert A. Brizzolara
IPC分类号： G01N33533
CPC分类号： G01N33/54386 , Y10S435/973 , Y10S977/857
摘要： Substrates are patterned with antibodies attached thereto at discrete locations from which absorption resistant coating is removed by selectively controlled mechanical scribing contact to avoid chemical removal so as to decrease fabrication costs and increase fabrication speed.
摘要翻译：在离散的位置对底物进行图案化，其中通过选择性地受控的机械划线接触除去吸收层，以避免化学去除，从而降低制造成本并提高制造速度。

10. 发明授权

US06200765B1 Non-invasive methods to detect prostate cancer 失效
标题翻译：非侵入性检测前列腺癌的方法
公开(公告)号：US06200765B1
公开(公告)日：2001-03-13
申请号：US09073213
申请日：1998-05-04
申请人： Gerald P. Murphy , Alton L. Boynton , Eric H. Holmes , Robert J. Barren, III
发明人： Gerald P. Murphy , Alton L. Boynton , Eric H. Holmes , Robert J. Barren, III
IPC分类号： G01N33533
CPC分类号： G01N33/57434
摘要： The present invention is directed to methods of detecting prostate cancer in a sample of a body fluid with prostate cell marker-specific and epithelial cell marker-specific antibodies as well as to kits comprising such antibodies for use in the detection of prostate cancer. The present invention is also directed to methods of detecting prostate cancer in a sample of a body fluid with prostate cell marker-specific and tumor associated marker-specific antibodies as well as to kits comprising such antibodies for use in the detection of prostate cancer.
摘要翻译：本发明涉及用前列腺细胞标记特异性和上皮细胞标志物特异性抗体检测体液样品中前列腺癌的方法，以及包含用于检测前列腺癌的抗体的试剂盒。本发明还涉及用前列腺细胞标志物特异性和肿瘤相关标志物特异性抗体检测体液样品中前列腺癌的方法，以及包含用于检测前列腺癌的抗体的试剂盒。

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