专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明授权

US06243980B1 Protease inhibitor assay 失效
标题翻译：蛋白酶抑制剂测定
公开(公告)号：US06243980B1
公开(公告)日：2001-06-12
申请号：US09035820
申请日：1998-03-06
申请人： Irena Bronstein , John Voyta , Michelle Palmer , Bonnie Tillotson
发明人： Irena Bronstein , John Voyta , Michelle Palmer , Bonnie Tillotson
IPC分类号： G01N33533
CPC分类号： G01N33/582 , C12Q1/37 , G01N33/581 , G01N2333/81 , Y10S436/80 , Y10S436/805
摘要： Heterogenous and homogenous assays are provided for the detection of protease inhibitory activity in a sample or target compound, taking advantage of the chemiluminescent characteristics of 1,2-dioxetanes. In the heterogenous assay, a peptide bearing a cleavage site for the protease of interest is provided with a first member of a first ligand binding pair at one end, and a first member of a second ligand binding pair at the other end. The other member of the first ligand binding pair is attached to a surface, which binds the peptide, or protease substrate, to the surface. The peptide substrate is combined with the protease and target compound or sample. Substrate cleavage, if not inhibited, is allowed to occur, and any unbound cleaved fragments are removed. An enzyme complexed with the second member of the second ligand binding pair is added, and allowed to bind to any of the (uncleaved) first member of the second ligand binding pair remaining. Unbound complex is removed, and a 1,2-dioxetane substrate for the enzyme is added. If any peptide substrate has not been cleaved, the dioxetane will chemiluminesce, indicating inhibitory activity. In a homogenous assay, the same substrate bears at one end a fluorescent energy accepting moiety, and at the other end a 1,2-dioxetane or precursor. If the substrate is cleaved by the protease, the dioxetane and the fluorescent moiety are not in close physical relationship, and no energy transfer occurs when the dioxetane is caused to decompose. If cleavage has not occurred, indicating inhibition, when the dioxetane is caused to decompose, energy is transferred to the fluorescing entity, which releases light of a wavelength recognizably distinct from that of the dioxetane.
摘要翻译：提供异源和均一的测定法，用于检测样品或目标化合物中蛋白酶抑制活性，利用1,2-二氧杂环己烷的化学发光特性。在异源测定中，具有目的蛋白酶的切割位点的肽在一端具有第一配体结合对的第一个成员，另一端具有第二配体结合对的第一个成员。第一配体结合对的另一个成员连接到表面，其将肽或蛋白酶底物结合到表面。将肽底物与蛋白酶和目标化合物或样品组合。如果不抑制底物裂解，则可以发生底物裂解，并且去除任何未结合的切割的片段。加入与第二配体结合对的第二个成员配合的酶，并使其与残留的第二配体结合对的任何（未切割的）第一个成员结合。去除未结合的络合物，并加入酶的1,2-二氧环乙烷底物。如果任何肽底物未被切割，二氧杂环丁烷将化学发光，表明抑制活性。在同质测定中，相同的底物在一端承载荧光能量接受部分，另一端在1,2-二氧杂环丁烷或前体上。如果底物被蛋白酶切割，二氧杂环丁烷和荧光部分不是很密切的物理关系，当二氧杂环丁烷分解时，不会发生能量转移。如果没有发生裂解，表明抑制，当二氧杂环丁烷分解时，能量被转移到荧光实体，其释放与二氧杂环丁烷的波长可辨别的波长的光。

2. 发明授权

US5800999A Dioxetane-precursor-labeled probes and detection assays employing the same 失效
标题翻译：二氧杂环丁烷 - 前体标记的探针和使用其的检测测定
公开(公告)号：US5800999A
公开(公告)日：1998-09-01
申请号：US767479
申请日：1996-12-16
申请人： Irena Bronstein , Brooks Edwards , Christopher Martin , John Voyta
发明人： Irena Bronstein , Brooks Edwards , Christopher Martin , John Voyta
IPC分类号： G01N33/532 , C07D321/00 , C07H21/00 , C07H21/02 , C07H21/04 , C12N15/09 , C12Q1/68 , C12N15/11
CPC分类号： C07H21/00 , C07D321/00 , C12Q1/6816 , C12Q1/686 , Y02P20/55
摘要： Probes labeled with 1,2-dioxetane precursors can be employed in a variety of assays. The probes may be nucleic acid, peptide nucleic acid, proteins including enzyme, antibody or antigen, steroid, carbohydrate, drug or non-drug hapten. The probe is provided with a 1,2-dioxetane precursor bound thereto, generally either covalently, or a strong ligand bond. The dioxetane precursor moiety is converted to a bound 1,2-dioxetane by exposure to singlet oxygen. These dioxetane (labels) either spontaneously decompose, or are induced to decompose by an appropriate trigger to release light. The trigger may be a change in pH temperature, or an agent which removes a protective group. Assay formats in which these 1,2-dioxetane labeled probes and referents may be used to include hybridization assays, immuno assays, gel-based assays and Capillary Zone Electrophoresis.
摘要翻译：用1,2-二氧杂环丁烷前体标记的探针可用于多种测定。探针可以是核酸，肽核酸，蛋白质，包括酶，抗体或抗原，类固醇，碳水化合物，药物或非药物半抗原。探针具有与其结合的1,2-二氧杂环丁烷前体，通常共价或强配体键。通过暴露于单线态氧将二氧杂环丁烷前体部分转化为结合的1,2-二氧环乙烷。这些二氧杂环丁烷（标记）会自发分解，或被适当的触发剂诱导分解以释放光。触发可能是pH温度的变化，或者是除去保护基团的试剂。这些1,2-二氧杂环丁烷标记的探针和指示物可用于包括杂交测定，免疫测定，基于凝胶的测定和毛细管区域电泳的测定形式。

3. 发明授权

US5753436A Chemiluminescent energy transfer assays 失效
公开(公告)号：US5753436A
公开(公告)日：1998-05-19
申请号：US401285
申请日：1995-03-09
申请人： Irena Bronstein , Brooks Edwards , John Voyta
发明人： Irena Bronstein , Brooks Edwards , John Voyta
IPC分类号： G01N33/531 , C07D321/00 , C12Q1/34 , G01N33/532 , G01N33/535 , G01N33/543 , G01N33/58 , G01N33/53
CPC分类号： G01N33/581 , C07D321/00 , C12Q1/34 , G01N33/535 , Y10S435/966 , Y10S435/968 , Y10S435/975
摘要： A chemiluminescent assays for the determination of the presence or amount of a biopolymer in bound assays using 1,2-dioxetanes in connection with AttoPhos.TM. as chemiluminescent substrates for enzyme-labeled targets or probes is provided. Further disclosed is a kit for conducting a bioassay for the presence or concentration of a biopolymer comprising a) an enzyme complex; b) a 1,2-dioxetane; and c) AttoPhos.TM..

4. 发明授权

US06287767B1 Chemiluminescent energy transfer assays 有权
标题翻译：化学发光能量转移测定
公开(公告)号：US06287767B1
公开(公告)日：2001-09-11
申请号：US09190181
申请日：1998-11-12
申请人： Irena Bronstein , Brooks Edwards , John Voyta
发明人： Irena Bronstein , Brooks Edwards , John Voyta
IPC分类号： G01N3353
CPC分类号： G01N33/581 , C07D321/00 , C12Q1/34 , G01N33/535 , Y10S435/966 , Y10S435/968 , Y10S435/975
摘要： A chemiluminescent assays for the determination of the presence or amount of a biopolymer in bound assays using 1,2-dioxetanes in connection with AttoPhos™ as chemiluminescent substrates for enzyme-labeled targets or probes is provided. Further disclosed is a kit for conducting a bioassay for the presence or concentration of a biopolymer comprising a) an enzyme complex; b) a 1,2-dioxetane; and c) AttoPhos™.
摘要翻译：提供了一种化学发光测定法，用于在结合测定中测定生物聚合物的存在或数量，使用与作为酶标记的靶标或探针的化学发光底物的AttoPhos TM相关的1,2-二氧杂环己烷。还公开了用于进行生物聚合物的存在或浓缩的试剂盒，其包含a）酶复合物; b）1,2-二氧环乙烷; 和c）AttoPhos TM。

5. 发明授权

US6063574A Dioxetane labeled probes and detection assays employing the same 失效
公开(公告)号：US6063574A
公开(公告)日：2000-05-16
申请号：US18180
申请日：1998-02-03
申请人： Irena Bronstein , Brooks Edwards , Christopher Martin , John Voyta
发明人： Irena Bronstein , Brooks Edwards , Christopher Martin , John Voyta
IPC分类号： G01N33/532 , C07D321/00 , C07H21/00 , C07H21/02 , C07H21/04 , C12N15/09 , C12Q1/68 , C12N15/11
CPC分类号： C07H21/00 , C07D321/00 , C12Q1/6816 , C12Q1/686 , Y02P20/55
摘要： Probes labeled with 1,2-dioxetane precursors can be employed in a variety of assays. The probes may be nucleic acid, peptide nucleic acid, proteins including enzyme, antibody or antigen, steroid, carbohydrate, drug or non-drug hapten. The probe is provided with a 1,2-dioxetane precursor bound thereto, generally either covalently, or a strong ligand bond. The dioxetane precursor moiety is converted to a bound 1,2-dioxetane by exposure to singlet oxygen. These dioxetane (labels) either spontaneously decompose, or are induced to decompose by an appropriate trigger to release light. The trigger may be a change in pH temperature, or an agent which removes a protective group. Assay formats in which these 1,2-dioxetane labeled probes and referents may be used to include hybridization assays, immuno assays, gel-based assays and Capillary Zone Electrophoresis.

6. 发明授权

US06451531B1 Dioxetane labeled probes and detection assays employing the same 有权
标题翻译：二氧杂环丁烷标记的探针和使用其的检测测定
公开(公告)号：US06451531B1
公开(公告)日：2002-09-17
申请号：US09340726
申请日：1999-06-29
申请人： Irena Bronstein , Brooks Edwards , Christopher Martin , John Voyta
发明人： Irena Bronstein , Brooks Edwards , Christopher Martin , John Voyta
IPC分类号： C12Q168
CPC分类号： C07H21/00 , C07D321/00 , C12Q1/6816 , C12Q1/686 , Y02P20/55 , C12Q2561/101 , C12Q2565/113
摘要： Probes labeled with 1,2-dioxetane precursors can be employed in a variety of assays. The probes may be nucleic acid, peptide nucleic acid, proteins including enzyme, antibody or antigen, steroid, carbohydrate, drug or non-drug hapten. The probe is provided with a 1,2-dioxetane precursor bound thereto, generally either covalently, or a strong ligand bond. The dioxetane precursor moiety is converted to a bound 1,2-dioxetane by exposure to singlet oxygen. These dioxetane (labels) either spontaneously decompose, or are induced to decompose by an appropriate trigger to release light. The trigger may be a change in pH temperature, or an agent which removes a protective group. Assay formats in which these 1,2-dioxetane labeled probes and referents may be used to include hybridization assays, immuno assays, gel-based assays and Capillary Zone Electrophoresis.
摘要翻译：用1,2-二氧杂环丁烷前体标记的探针可用于多种测定。探针可以是核酸，肽核酸，蛋白质，包括酶，抗体或抗原，类固醇，碳水化合物，药物或非药物半抗原。探针具有与其结合的1,2-二氧杂环丁烷前体，通常共价或强配体键。通过暴露于单线态氧将二氧杂环丁烷前体部分转化为结合的1,2-二氧环乙烷。这些二氧杂环丁烷（标记）会自发分解，或被适当的触发剂诱导分解以释放光。触发可能是pH温度的变化，或者是除去保护基团的试剂。这些1,2-二氧杂环丁烷标记的探针和指示物可用于包括杂交测定，免疫测定，基于凝胶的测定和毛细管区域电泳的测定形式。

7. 发明授权

US5336596A Membrane for chemiluminescent blotting applications 失效
标题翻译：用于化学发光印迹应用的膜
公开(公告)号：US5336596A
公开(公告)日：1994-08-09
申请号：US811620
申请日：1991-12-23
申请人： Irena Bronstein , Brooks Edwards , John Voyta
发明人： Irena Bronstein , Brooks Edwards , John Voyta
IPC分类号： G01N21/78 , B32B27/30 , C09D125/00 , C09D125/18 , G01N33/545 , G01N33/58 , C12B1/68 , B32B27/00
CPC分类号： G01N33/582 , G01N33/545 , Y10T428/31536 , Y10T428/31743 , Y10T428/31884
摘要： A new and improved polymeric membrane for use in biological assays is provided. A blotting assay employing 1,2-dioxetanes as a source of chemiluminescence employs, as an improved membrane, a polymer comprised of at least one monomer of the formula: ##STR1## The membranes reduce background signal, improve sensitivity and reliability.
摘要翻译：提供了用于生物测定的新的和改进的聚合物膜。使用1,2-二氧杂环丁烷作为化学发光源的印迹测定使用由至少一种下式的单体组成的聚合物作为改进的膜：提高了灵敏度和可靠性。

8. 发明授权

US07053208B2 Dioxetane labeled probes and detection assays employing the same 有权
公开(公告)号：US07053208B2
公开(公告)日：2006-05-30
申请号：US10083474
申请日：2002-02-27
申请人： Irena Bronstein , Brooks Edwards , Christopher Martin , John Voyta
发明人： Irena Bronstein , Brooks Edwards , Christopher Martin , John Voyta
IPC分类号： C12Q1/68 , C07H21/00 , C12M1/00 , C12M1/34 , C12M3/00
CPC分类号： C07H21/00 , C07D321/00 , C12Q1/6816 , C12Q1/686 , Y02P20/55 , C12Q2561/101 , C12Q2565/113
摘要： Probes labeled with 1,2-dioxetane precursors can be employed in a variety of assays. The probes may be nucleic acid, peptide nucleic acid, proteins including enzyme, antibody or antigen, steroid, carbohydrate, drug or non-drug hapten. The probe is provided with a 1,2-dioxetane precursor bound thereto, generally either covalently, or a strong ligand bond. The dioxetane precursor moiety is converted to a bound 1,2-dioxetane by exposure to singlet oxygen. These dioxetane (labels) either spontaneously decompose, or are induced to decompose by an appropriate trigger to release light. The trigger may be a change in pH temperature, or an agent which removes a protective group. Assay formats in which these 1,2-dioxetane labeled probes and referents may be used to include hybridization assays, immuno assays, gel-based assays and Capillary Zone Electrophoresis.

9. 发明授权

US6162610A Xanthan-ester and acridan substrates for horseradish peroxidase 失效
标题翻译：黄原酸酯和辣根过氧化物酶的吖啶底物
公开(公告)号：US6162610A
公开(公告)日：2000-12-19
申请号：US152247
申请日：1998-09-11
申请人： Irena Bronstein , Brooks Edwards , John Voyta , Rouh-Rong Juo
发明人： Irena Bronstein , Brooks Edwards , John Voyta , Rouh-Rong Juo
IPC分类号： G01N33/53 , C07D219/06 , C07D311/84 , C09K11/07 , C12Q1/28 , G01N21/76 , G01N33/535 , G01N33/577 , G01N33/533 , C07D219/04 , C07D311/82 , C07D335/12 , C12N9/96
CPC分类号： C07D219/06 , C07D311/84 , C12Q1/28 , G01N2333/59 , Y10S435/968
摘要： Xanthan esters and acridans are substrates for horseradish peroxidase. These stable, enzymatically cleavable chemiluminescent esters are substrates for horseradish peroxidase which, together with peroxide is among the extensively used enzyme in enzyme-linked detection methods, including immunoassays, oligonucleotide detection and nucleic acid hybridization. The novel compounds are used, together with peroxide, alkali and the peroxidase, to indicate the presence and/or concentration of target compounds. The assays may be enhanced by the use of polymeric quaternary onium enhancement compounds or similar compounds selected to enhance the chemiluminescence emitted.
摘要翻译：黄原胶和吖啶是辣根过氧化物酶的底物。这些稳定的，酶促切割的化学发光酯是辣根过氧化物酶的底物，与过氧化物一起是酶联检测方法中广泛使用的酶，包括免疫测定，寡核苷酸检测和核酸杂交。这些新型化合物与过氧化物，碱和过氧化物酶一起使用，以表明目标化合物的存在和/或浓度。可以通过使用聚合季铵增强化合物或选择用于增强所发射的化学发光的类似化合物来增强测定。

10. 发明授权

US5849495A Chemiluminescent energy transfer assays 失效
标题翻译：化学发光能量转移测定
公开(公告)号：US5849495A
公开(公告)日：1998-12-15
申请号：US811539
申请日：1997-03-04
申请人： Irena Bronstein , Brooks Edwards , John Voyta
发明人： Irena Bronstein , Brooks Edwards , John Voyta
IPC分类号： G01N33/531 , C07D321/00 , C12Q1/34 , G01N33/532 , G01N33/535 , G01N33/543 , G01N33/58 , G01N33/53
CPC分类号： G01N33/581 , C07D321/00 , C12Q1/34 , G01N33/535 , Y10S435/966 , Y10S435/968 , Y10S435/975
摘要： A chemiluminescent assays for the determination of the presence or amount of a biopolymer in bound assays using 1,2-dioxetanes in connection with AttoPhos.TM. as chemiluminescent substrates for enzyme-labeled targets or probes is provided. Further disclosed is a kit for conducting a bioassay for the presence or concentration of a biopolymer comprising a) an enzyme complex; b) a 1,2-dioxetane; and c) AttoPhos.TM..
摘要翻译：提供了一种化学发光测定法，用于测定使用与作为酶标记靶或探针的化学发光底物的AttoPhos TM相关的1,2-二氧杂环己烷的结合测定中的生物聚合物的存在或量。还公开了用于进行生物聚合物的存在或浓缩的试剂盒，其包含a）酶复合物; b）1,2-二氧环乙烷; 和c）AttoPhos TM。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式