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1. 发明授权

US06632603B1 Non-captive substrate liquid phase immunoassay 失效
标题翻译：非捕获底物液相免疫测定
公开(公告)号：US06632603B1
公开(公告)日：2003-10-14
申请号：US08914700
申请日：1997-08-19
申请人： Thomas T. Hubscher , Erik P. Lillehoj
发明人： Thomas T. Hubscher , Erik P. Lillehoj
IPC分类号： G01N33533
CPC分类号： G01N33/585 , B01L3/5023 , B01L2300/0825 , B01L2400/0406 , G01N33/538 , G01N2333/4737 , Y10S435/971 , Y10S435/975 , Y10S436/824
摘要： An immunoassay of a liquid biological specimen for a specific analyte is conducted by forming a test sample by contacting the specimen with: (1) a binding substance of a ligand, antiligand or receptor capable of binding the analyte and (2) a detector substance of a colloidal gold or silver labeled ligand or antiligand to form a test sample. The test sample is applied by flowing onto a defined zone of an insoluble porous support film having a pore size impassable to a complex formed between the analyte, if present, with the binding substance and the detector substance, but passable to the binding substance and detector substance while remaining uncomplexed in the absence of the desired analyte. If the analyte is present in the test specimen, the detector substance binds with the analyte and the binding substance to form a visually inspectable complex on the surface of the porous support film. After application of the test sample to the porous support, the surface of the porous support is visually inspected for color to determine the presence and quantity or the absence of the analyte being assayed.
摘要翻译：对特定分析物的液体生物样品进行免疫测定，通过使样品与以下物质接触来形成测试样品：（1）能够结合分析物的配体，反配体或受体的结合物质和（2）检测物质胶体金或银标记的配体或反配体以形成测试样品。通过流动到不溶性多孔载体膜的限定区域上来施加测试样品，所述不溶性多孔载体膜的孔隙尺寸不能通过分析物（如果存在）与结合物质和检测物质形成的复合物通过，但可通过结合物质和检测器物质，同时在不存在所需分析物的情况下保持未络合。如果分析物存在于测试样品中，检测器物质与分析物和结合物质结合，以在多孔载体膜的表面上形成目视检查的复合物。在将测试样品施加到多孔载体上之后，目视检查多孔载体的表面的颜色，以确定待分析物的存在和数量或不存在。

2. 发明授权

US06528325B1 Method for the visual detection of specific antibodies in human serum by the use of lateral flow assays 有权
标题翻译：通过使用侧流测定法在人血清中目视检测特异性抗体的方法
公开(公告)号：US06528325B1
公开(公告)日：2003-03-04
申请号：US09689682
申请日：2000-10-13
申请人： Thomas T. Hubscher , Glen M Ford , Teri M Ruppenthal
发明人： Thomas T. Hubscher , Glen M Ford , Teri M Ruppenthal
IPC分类号： G01N33543
CPC分类号： G01N33/558 , Y10S435/97 , Y10S435/975 , Y10S436/807 , Y10S436/81 , Y10S436/821
摘要： This invention discloses a method and composition for detecting the presence of class specific antibodies reactive with analytes such as bacteria, allergens, autoimmune antigens, viral proteins, and carbohydrates by lateral flow techniques. In one embodiment of the invention, a test sample obtained from bodily fluids reacts with a gold labeled antigen. The resulting complex travels across the membrane, and along the lateral flow strip. Red colored lines formed in specific locations along the test strip indicate the presence of class specific antibodies in the test specimen. In another embodiment of the invention, the lateral flow assay serves as an immunochromatographic screening test for the detection of allergen-specific IgE antibodies in human serum. Test sample reacts with gold labeled anti-IgE antibody. The resulting complex travels across the membrane where immobilized allergens capture the allergen specific IgE complex. Colored lines are formed in the test areas to indicate the presence of allergen-specific IgE antibodies.
摘要翻译：本发明公开了一种通过侧流技术检测与分析物如细菌，过敏原，自身免疫抗原，病毒蛋白质和碳水化合物反应的类别特异性抗体的存在的方法和组合物。在本发明的一个实施方案中，从体液获得的测试样品与金标记的抗原反应。所得的复合物穿过膜并且沿着横向流动条移动。沿着测试条形成在特定位置的红色着色线表示试样中存在类别特异性抗体。在本发明的另一个实施方案中，侧流测定用作人血清中检测变应原特异性IgE抗体的免疫色谱筛选试验。测试样品与金标记的抗IgE抗体反应。所得复合物穿过膜，其中固定的变应原捕获变应原特异性IgE复合物。在测试区域中形成着色线，以指示过敏原特异性IgE抗体的存在。

3. 发明授权

US5244788A Method and apparatus for performing determinations of immune rectants in biological fluids 失效
标题翻译：用于在生物体液中测定免疫整流剂的方法和装置
公开(公告)号：US5244788A
公开(公告)日：1993-09-14
申请号：US907355
申请日：1992-07-01
申请人： Thomas T. Hubscher
发明人： Thomas T. Hubscher
IPC分类号： G01N33/53 , G01N33/543
CPC分类号： G01N33/54366 , G01N33/5308
摘要： A method and apparatus for performing determinations of immune reactants (e.g., antigens, antibodies) in bodily fluids utilize multiple test units having respective elongated rods with transversely-expanded polymer tips at their distal ends. The tip surfaces contain microgrooves and are coated with respective immune reactants (e.g., allergens) of the type which react in a known manner with respective allergen-specific or allergen-binding antibodies in human serum. The supporting strip for the test units has through-holes which frictionally or adhesively engage the proximal ends of the test unit rods with a spacing that permits all of the supported test units to be simultaneously inserted into a reaction vessel permitting simultaneous determination of reactants and degrees thereof against multiple immune reactants of varying kinds (i.e., allergens, antigens, antibodies) in a common sample of body fluid (i.e., serum, plasma, whole blood, etc.). The shallow microgrooves on the test tip permit rapid penetration of dye and rinsing of the test units. After incubation in the sample fluid, the test units are washed and then incubate in an enzyme-antibody conjugate. Upon additional rinsing the test units are permitted to incubate in a chromogenic substrate specific to the enzyme. The intensity of the resulting color (typically blue) on the tip is a measure of the amount of specific antibodies in the tested fluid sample.
摘要翻译：用于在体液中进行免疫反应物（例如，抗原，抗体）的测定的方法和装置利用具有在其远端具有横向膨胀的聚合物末端的相应细长杆的多个测试单元。尖端表面包含微槽，并且涂覆有各种免疫反应物（例如变应原），其以已知方式与人血清中各自的变应原特异性或变应原结合抗体反应。用于测试单元的支撑条具有通孔，其以测试单位杆的近端摩擦或粘合地接合的间隙，允许所有受支撑的测试单元同时插入反应容器中，允许同时测定反应物和度其针对体液（即，血清，血浆，全血等）的常见样品中的多种免疫反应物（即，变应原，抗原，抗体）。测试头上的浅微槽允许染料快速渗透并冲洗测试单元。在样品液中孵育后，洗涤测试单元，然后在酶 - 抗体缀合物中孵育。在额外冲洗时，允许测试单元在特异于酶的显色底物中孵育。尖端上产生的颜色（通常为蓝色）的强度是测试流体样品中特异性抗体量的量度。

4. 发明申请

US20100068797A1 Method for the visual detection of specific antibodies by the use of lateral flow assays 审中-公开
标题翻译：通过使用侧流测定法可视化检测特异性抗体的方法
公开(公告)号：US20100068797A1
公开(公告)日：2010-03-18
申请号：US12458868
申请日：2009-07-24
申请人： Thomas T. Hubscher
发明人： Thomas T. Hubscher
IPC分类号： C12M1/34 , G01N33/53
CPC分类号： G01N33/558 , G01N2800/52 , Y10S435/97 , Y10S435/975 , Y10S436/807 , Y10S436/81 , Y10S436/821
摘要： This disclosure teaches a method and composition for detecting the presence of class specific antibodies reactive with analytes such as bacteria, allergens, autoimmune antigens, viral proteins, haptens, and carbohydrates by lateral flow techniques. In one embodiment of the disclosure, a test sample is added to the test strip, which then migrates to the site of the immobilized allergens, thereby forming a first antibody IgE-allergen complex. A chase release buffer is added upstream to or from the site of the labeled anti-IgE antibodies, which is itself upstream from the sample site. The anti-IgE antibodies migrate downstream to the site of immobilized first complex, thereby forming a second complex indicating the presence of class specific IgE antibodies in the test specimen. In another embodiment of the disclosure, a liquid form of the labeled anti-IgE antibodies can be added to the test strip after the first complex has been formed.These techniques can be used to perform other lateral flow tests.
摘要翻译：本公开教导了用于通过侧流技术检测与分析物例如细菌，变应原，自身免疫抗原，病毒蛋白，半抗原和碳水化合物反应的类别特异性抗体的存在的方法和组合物。在本公开的一个实施方案中，将测试样品加入到测试条中，然后将其移植到固定的变应原的位点，从而形成第一抗体IgE-变应原复合物。在标记的抗IgE抗体的位置上游或从其位点加入追踪释放缓冲液，其本身在样品位点的上游。抗IgE抗体向下游迁移到固定化的第一复合物的位点，从而形成第二个复合物，表明在试样中存在类别特异性IgE抗体。在本公开的另一个实施方案中，在形成第一配合物之后，可以将标记的抗IgE抗体的液体形式加入到测试条中。这些技术可用于执行其他侧向流动测试。

5. 发明授权

US5494830A Methods for performing determinations of immune reactants in biological fluids 失效
标题翻译：在生物流体中进行免疫反应物测定的方法
公开(公告)号：US5494830A
公开(公告)日：1996-02-27
申请号：US296209
申请日：1994-08-25
申请人： Thomas T. Hubscher
发明人： Thomas T. Hubscher
IPC分类号： G01N33/53 , G01N33/543
CPC分类号： G01N33/54366 , G01N33/5302 , G01N33/5304 , Y10S435/81 , Y10S435/973 , Y10S436/805 , Y10S436/807 , Y10S436/809 , Y10S436/81
摘要： Apparatus for performing determinations of immune reactants (e.g., antigens, antibodies) in bodily fluids includes multiple test units having respective elongated rods with transversely-expanded tips at their distal ends. The tips are each coated with respective immune reactants (e.g., allergens) which react in a known manner with respective allergen-specific or allergen-binding antibodies in human serum. The test units are color-coded to identify the allergen coatings and are supported at their proximal ends and positionally identified on a strip. By correlating the color code to a chart, the specific immune reactant (e.g., allergen) coating can be easily identified. The supporting strip for the test unit has through-holes which frictionally engage the proximal ends of the test unit rods with a spacing that permits all of the supported test units to be simultaneously inserted into an assembly of reaction containers arranged in a linear array. Additionally, the through-hole spacing in the strip permits alternate test units to be simultaneously placed in an array of test tubes that are transversely larger than the reaction containers and supported in a linear array in a test tube rack. The tip of each test unit may take the form of two frusto-conical sections joined by a short cylindrical section extending longitudinally between their larger ends. Alternatively, the frusto-conical sections may be multi-faceted to minimize scraping of the allergen coating from the tip during insertion and removal of the test units into the reaction containers/test tubes.
摘要翻译：用于在体液中测定免疫反应物（例如抗原，抗体）的装置包括多个测试单元，其具有在其远端具有横向扩张的尖端的相应细长杆。每个末端各自涂覆有各自的免疫反应物（例如变应原），其以已知方式与人血清中各自的变应原特异性或变应原结合抗体反应。测试单元采用颜色编码，以识别变应原涂层，并在其近端支撑并在条带上位置识别。通过将颜色代码与图表相关联，可以容易地鉴定特异性免疫反应物（例如变应原）涂层。用于测试单元的支撑条具有通孔，该通孔与测试单元棒的近端摩擦接合，间隔允许所有受支撑的测试单元同时插入到以线性阵列布置的反应容器的组件中。此外，条带中的通孔间隔允许替代的测试单元同时放置在横向大于反应容器并且以线性阵列支撑在试管架中的试管阵列中。每个测试单元的尖端可以采用通过在其较大端部之间纵向延伸的短圆柱形部分连接的两个截头圆锥形部分的形式。或者，截头圆锥形部分可以是多面的，以在插入和移除测试单元进入反应容器/试管期间最小化从尖端刮去变应原涂层。

6. 发明授权

US4891321A Apparatus for performing determinations of immune reactants in biological fluids 失效
标题翻译：用于在生物流体中测定免疫反应物的装置
公开(公告)号：US4891321A
公开(公告)日：1990-01-02
申请号：US110846
申请日：1987-10-21
申请人： Thomas T. Hubscher
发明人： Thomas T. Hubscher
IPC分类号： G01N33/53 , G01N33/543
CPC分类号： G01N33/54366 , G01N33/5302 , G01N33/5304 , Y10S435/81 , Y10S435/973 , Y10S436/805 , Y10S436/807 , Y10S436/809 , Y10S436/81
摘要： Apparatus for performing determinations of immune reactants (e.g., antigens, antibodies) in bodily fluids includes multiple test units having respective elongated rods with transversely-expanded tips at their distal ends. The tips are each coated with respective immune reactants (e.g., allergens of the type which react in a known manner with respective allergen-specific or allergen-binding antibodies in human serum. The test units are color-coded. By correlating the color code to a chart, the specific immune reactant (e.g., allergen) coating can be easily identified. The supporting strip for the test unit has through-holes which frictionally engage the proximal ends of the test unit rods with a spacing that permits all of the supported test units to be simultaneously inserted into an assembly of reaction containers arranged in a linear array. The through-hole spacing in the strip permits alternate test units to be simultaneously placed in an array of test tubes that are transversely larger than the reaction containers and supported in a linear array in a test tube rack. The tip of each test unit may take the form of two frusto-conical joined by a short cylindrical section extending longitudinally beween their larger ends. The frusto-conical sections may be multi-faceted to minimize scraping of the allergen coating from the tip during insertion and removal of the test units into the reaction containers/test tubes.
摘要翻译：用于在体液中测定免疫反应物（例如抗原，抗体）的装置包括多个测试单元，其具有在其远端具有横向扩张的尖端的相应细长杆。每个末端各自涂覆有各自的免疫反应物（例如，以已知方式与人血清中各自的变应原特异性或变应原结合抗体反应的类型的变应原，测试单元是彩色编码的，通过将颜色代码与可以容易地识别特定的免疫反应物（例如变应原）涂层，用于测试单元的支撑条具有通孔，该通孔与测试单元棒的近端摩擦接合，间隔允许所有支持的测试单元同时插入到以线性阵列布置的反应容器的组件中。条带中的通孔间隔允许替代的测试单元同时放置在横向大于反应容器的试管阵列中，并支撑在每个测试单元的尖端可以采取两个截头圆锥形的形式，该两个截头圆锥体通过纵向延伸的短圆柱形部分连接他们的大端。截头圆锥形截面可以是多面的，以便在将测试单元插入和移除到反应容器/试管中时，最小化从尖端刮去变应原涂层。

7. 发明授权

US07629127B2 Method for the visual detection of specific antibodies by the use of lateral flow assays 有权
标题翻译：通过使用侧流测定法可视化检测特异性抗体的方法
公开(公告)号：US07629127B2
公开(公告)日：2009-12-08
申请号：US11038076
申请日：2005-01-21
申请人： Thomas T. Hubscher
发明人： Thomas T. Hubscher
IPC分类号： G01N33/53
CPC分类号： G01N33/558 , G01N2800/52 , Y10S435/97 , Y10S435/975 , Y10S436/807 , Y10S436/81 , Y10S436/821
摘要： This disclosure teaches a method and composition for detecting the presence of class specific antibodies reactive with analytes such as bacteria, allergens, autoimmune antigens, viral proteins, haptens, and carbohydrates by lateral flow techniques. In one embodiment of the disclosure, a test sample is added to the test strip, which then migrates to the site of the immobilized allergens, thereby forming a first antibody IgE-allergen complex. A chase release buffer is added upstream to or from the site of the labeled anti-IgE antibodies, which is itself upstream from the sample site. The anti-IgE antibodies migrate downstream to the site of immobilized first complex, thereby forming a second complex indicating the presence of class specific IgE antibodies in the test specimen. In another embodiment of the disclosure, a liquid form of the labeled anti-IgE antibodies can be added to the test strip after the first complex has been formed.
摘要翻译：本公开教导了用于通过横向流技术检测与分析物如细菌，变应原，自身免疫抗原，病毒蛋白，半抗原和碳水化合物反应的类别特异性抗体的存在的方法和组合物。在本公开的一个实施方案中，将测试样品加入到测试条中，然后将其移植到固定的变应原的位点，从而形成第一抗体IgE-变应原复合物。在标记的抗IgE抗体的位置上游或从其位点加入追踪释放缓冲液，其本身在样品位点的上游。抗IgE抗体向下游迁移到固定化的第一复合物的位点，从而形成第二个复合物，表明在试样中存在类别特异性IgE抗体。在本公开的另一个实施方案中，在形成第一配合物之后，可以将标记的抗IgE抗体的液体形式加入到测试条中。

8. 发明授权

US06824975B2 Incorporation of selective binding substances in a solid phase assay device 有权
标题翻译：在固相测定装置中引入选择性结合物质
公开(公告)号：US06824975B2
公开(公告)日：2004-11-30
申请号：US09974913
申请日：2001-10-12
申请人： Thomas T. Hubscher , Glen M Ford , Teri M Ruppenthal
发明人： Thomas T. Hubscher , Glen M Ford , Teri M Ruppenthal
IPC分类号： G01N3353
CPC分类号： G01N33/558 , Y10S435/97 , Y10S435/975 , Y10S436/807 , Y10S436/81 , Y10S436/821
摘要： This invention discloses a method and composition for detecting the presence of class specific antibodies reactive with analytes such as bacteria, allergens, autoimmune antigens, viral proteins, and carbohydrates by lateral flow techniques. In one embodiment of the invention, a test sample obtained from bodily fluids reacts with a gold labeled antigen. The resulting complex travels across the membrane, and along the lateral flow strip. Red colored lines formed in specific locations along the test strip indicate the presence of class specific antibodies in the test specimen. In another embodiment of the invention, the lateral flow assay serves as an immunochromatographic screening test for the detection of allergen-specific IgE antibodies in human serum. Test sample reacts with gold labeled anti-IgE antibody. The resulting complex travels across the membrane where immobilized allergens capture the allergen specific IgE complex. Colored lines are formed int the test areas to indicate the presence of allergen-specific IgE antibodies.
摘要翻译：本发明公开了一种通过侧流技术检测与分析物如细菌，过敏原，自身免疫抗原，病毒蛋白质和碳水化合物反应的类别特异性抗体的存在的方法和组合物。在本发明的一个实施方案中，从体液获得的测试样品与金标记的抗原反应。所得的复合物穿过膜并且沿着横向流动条移动。沿着测试条形成在特定位置的红色着色线表示试样中存在类别特异性抗体。在本发明的另一个实施方案中，侧流测定用作人血清中检测变应原特异性IgE抗体的免疫色谱筛选试验。测试样品与金标记的抗IgE抗体反应。所得复合物穿过膜，其中固定的变应原捕获变应原特异性IgE复合物。在测试区域内形成有色线，以指示过敏原特异性IgE抗体的存在。

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