专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明授权

US08278114B2 Method for measuring luminescence at a luminescence detection workstation 有权
标题翻译：用于在发光检测工作站测量发光的方法
公开(公告)号：US08278114B2
公开(公告)日：2012-10-02
申请号：US12716219
申请日：2010-03-02
申请人： Michael Gambini , Jeff Levi , John Voyta , John Atwood , Susan Atwood-Stone, legal representative , Bruce De Simas , Edward Lakatos , Israel Metal , George Sabak , Yongdong Wang
发明人： Michael Gambini , Jeff Levi , John Voyta , John Atwood , Bruce De Simas , Edward Lakatos , Israel Metal , George Sabak , Yongdong Wang
IPC分类号： G01N21/76 , G01T1/10
CPC分类号： G01N21/763 , G01N21/15 , G01N21/274 , G01N21/276 , G01N21/31 , G01N21/6452 , G01N21/6456 , G01N21/6486 , G01N21/76 , G01N33/581 , G01N2021/6471 , G01N2333/90241 , Y10T436/115831
摘要： A luminescence detecting apparatus and method for analyzing luminescent samples is disclosed. A detecting apparatus may be configured so that light from luminescent samples pass through a collimator, a a first lens, a filter, and a camera lens, whereupon an image is created by the optics on the charge-coupled device (CCD) camera. The detecting apparatus may further include central processing control of all operations, multiple wavelength filter wheel, and/or a robot for handling of samples and reagents.
摘要翻译：公开了一种用于分析发光样品的发光检测装置和方法。检测装置可以被配置为使得来自发光样品的光通过准直仪，第一透镜，滤光器和相机透镜，于是由电荷耦合器件（CCD）照相机上的光学器件产生图像。检测装置还可以包括用于处理样品和试剂的所有操作，多波长滤光轮和/或机器人的中央处理控制。

2. 发明申请

US20060088444A1 Luminescence detection workstation 有权
标题翻译：发光检测工作站
公开(公告)号：US20060088444A1
公开(公告)日：2006-04-27
申请号：US11251873
申请日：2005-10-18
申请人： Michael Gambini , John Voyta , John Atwood , Susan Atwood Stone , Bruce DeSimas , Edward Lakatos , Jeff Levi , Israel Metal , George Sabak , Yongdong Wang
发明人： Michael Gambini , John Voyta , John Atwood , Susan Atwood Stone , Bruce DeSimas , Edward Lakatos , Jeff Levi , Israel Metal , George Sabak , Yongdong Wang
IPC分类号： G01N21/64
CPC分类号： G01N21/763 , G01N21/15 , G01N21/274 , G01N21/276 , G01N21/31 , G01N21/6452 , G01N21/6456 , G01N21/6486 , G01N21/76 , G01N33/581 , G01N2021/6471 , G01N2333/90241 , Y10T436/115831
摘要： In a luminescence detecting apparatus and method for analyzing luminescent samples, luminescent samples are placed in a plurality of sample wells in a tray, and the tray is placed in a visible-light impervious chamber containing a charge coupled device camera. In the chamber, light from the luminescent samples pass through a collimator, a Fresnel field lens, an infrared filter, and a camera lens, whereupon a focused image is created by the optics on the camera. The use of an infrared filter suppresses stray IR radiation resulting from plate phosphorescence (which can result in abnormally high backgrounds and/or alteration of the image received by the camera).
摘要翻译：在用于分析发光样品的发光检测装置和方法中，将发光样品放置在托盘中的多个样品孔中，并将该托盘放置在包含电荷耦合器件相机的可见光不透过室中。在室中，来自发光样品的光通过准直仪，菲涅尔场透镜，红外滤光镜和照相机镜头，由相机上的光学器件产生聚焦图像。使用红外滤光器可抑制板状磷光产生的杂散红外辐射（可能导致摄像机接收的图像异常高的背景和/或变化）。

3. 发明授权

US5800999A Dioxetane-precursor-labeled probes and detection assays employing the same 失效
标题翻译：二氧杂环丁烷 - 前体标记的探针和使用其的检测测定
公开(公告)号：US5800999A
公开(公告)日：1998-09-01
申请号：US767479
申请日：1996-12-16
申请人： Irena Bronstein , Brooks Edwards , Christopher Martin , John Voyta
发明人： Irena Bronstein , Brooks Edwards , Christopher Martin , John Voyta
IPC分类号： G01N33/532 , C07D321/00 , C07H21/00 , C07H21/02 , C07H21/04 , C12N15/09 , C12Q1/68 , C12N15/11
CPC分类号： C07H21/00 , C07D321/00 , C12Q1/6816 , C12Q1/686 , Y02P20/55
摘要： Probes labeled with 1,2-dioxetane precursors can be employed in a variety of assays. The probes may be nucleic acid, peptide nucleic acid, proteins including enzyme, antibody or antigen, steroid, carbohydrate, drug or non-drug hapten. The probe is provided with a 1,2-dioxetane precursor bound thereto, generally either covalently, or a strong ligand bond. The dioxetane precursor moiety is converted to a bound 1,2-dioxetane by exposure to singlet oxygen. These dioxetane (labels) either spontaneously decompose, or are induced to decompose by an appropriate trigger to release light. The trigger may be a change in pH temperature, or an agent which removes a protective group. Assay formats in which these 1,2-dioxetane labeled probes and referents may be used to include hybridization assays, immuno assays, gel-based assays and Capillary Zone Electrophoresis.
摘要翻译：用1,2-二氧杂环丁烷前体标记的探针可用于多种测定。探针可以是核酸，肽核酸，蛋白质，包括酶，抗体或抗原，类固醇，碳水化合物，药物或非药物半抗原。探针具有与其结合的1,2-二氧杂环丁烷前体，通常共价或强配体键。通过暴露于单线态氧将二氧杂环丁烷前体部分转化为结合的1,2-二氧环乙烷。这些二氧杂环丁烷（标记）会自发分解，或被适当的触发剂诱导分解以释放光。触发可能是pH温度的变化，或者是除去保护基团的试剂。这些1,2-二氧杂环丁烷标记的探针和指示物可用于包括杂交测定，免疫测定，基于凝胶的测定和毛细管区域电泳的测定形式。

4. 发明授权

US5753436A Chemiluminescent energy transfer assays 失效
公开(公告)号：US5753436A
公开(公告)日：1998-05-19
申请号：US401285
申请日：1995-03-09
申请人： Irena Bronstein , Brooks Edwards , John Voyta
发明人： Irena Bronstein , Brooks Edwards , John Voyta
IPC分类号： G01N33/531 , C07D321/00 , C12Q1/34 , G01N33/532 , G01N33/535 , G01N33/543 , G01N33/58 , G01N33/53
CPC分类号： G01N33/581 , C07D321/00 , C12Q1/34 , G01N33/535 , Y10S435/966 , Y10S435/968 , Y10S435/975
摘要： A chemiluminescent assays for the determination of the presence or amount of a biopolymer in bound assays using 1,2-dioxetanes in connection with AttoPhos.TM. as chemiluminescent substrates for enzyme-labeled targets or probes is provided. Further disclosed is a kit for conducting a bioassay for the presence or concentration of a biopolymer comprising a) an enzyme complex; b) a 1,2-dioxetane; and c) AttoPhos.TM..

5. 发明授权

US06287767B1 Chemiluminescent energy transfer assays 有权
标题翻译：化学发光能量转移测定
公开(公告)号：US06287767B1
公开(公告)日：2001-09-11
申请号：US09190181
申请日：1998-11-12
申请人： Irena Bronstein , Brooks Edwards , John Voyta
发明人： Irena Bronstein , Brooks Edwards , John Voyta
IPC分类号： G01N3353
CPC分类号： G01N33/581 , C07D321/00 , C12Q1/34 , G01N33/535 , Y10S435/966 , Y10S435/968 , Y10S435/975
摘要： A chemiluminescent assays for the determination of the presence or amount of a biopolymer in bound assays using 1,2-dioxetanes in connection with AttoPhos™ as chemiluminescent substrates for enzyme-labeled targets or probes is provided. Further disclosed is a kit for conducting a bioassay for the presence or concentration of a biopolymer comprising a) an enzyme complex; b) a 1,2-dioxetane; and c) AttoPhos™.
摘要翻译：提供了一种化学发光测定法，用于在结合测定中测定生物聚合物的存在或数量，使用与作为酶标记的靶标或探针的化学发光底物的AttoPhos TM相关的1,2-二氧杂环己烷。还公开了用于进行生物聚合物的存在或浓缩的试剂盒，其包含a）酶复合物; b）1,2-二氧环乙烷; 和c）AttoPhos TM。

6. 发明授权

US06243980B1 Protease inhibitor assay 失效
标题翻译：蛋白酶抑制剂测定
公开(公告)号：US06243980B1
公开(公告)日：2001-06-12
申请号：US09035820
申请日：1998-03-06
申请人： Irena Bronstein , John Voyta , Michelle Palmer , Bonnie Tillotson
发明人： Irena Bronstein , John Voyta , Michelle Palmer , Bonnie Tillotson
IPC分类号： G01N33533
CPC分类号： G01N33/582 , C12Q1/37 , G01N33/581 , G01N2333/81 , Y10S436/80 , Y10S436/805
摘要： Heterogenous and homogenous assays are provided for the detection of protease inhibitory activity in a sample or target compound, taking advantage of the chemiluminescent characteristics of 1,2-dioxetanes. In the heterogenous assay, a peptide bearing a cleavage site for the protease of interest is provided with a first member of a first ligand binding pair at one end, and a first member of a second ligand binding pair at the other end. The other member of the first ligand binding pair is attached to a surface, which binds the peptide, or protease substrate, to the surface. The peptide substrate is combined with the protease and target compound or sample. Substrate cleavage, if not inhibited, is allowed to occur, and any unbound cleaved fragments are removed. An enzyme complexed with the second member of the second ligand binding pair is added, and allowed to bind to any of the (uncleaved) first member of the second ligand binding pair remaining. Unbound complex is removed, and a 1,2-dioxetane substrate for the enzyme is added. If any peptide substrate has not been cleaved, the dioxetane will chemiluminesce, indicating inhibitory activity. In a homogenous assay, the same substrate bears at one end a fluorescent energy accepting moiety, and at the other end a 1,2-dioxetane or precursor. If the substrate is cleaved by the protease, the dioxetane and the fluorescent moiety are not in close physical relationship, and no energy transfer occurs when the dioxetane is caused to decompose. If cleavage has not occurred, indicating inhibition, when the dioxetane is caused to decompose, energy is transferred to the fluorescing entity, which releases light of a wavelength recognizably distinct from that of the dioxetane.
摘要翻译：提供异源和均一的测定法，用于检测样品或目标化合物中蛋白酶抑制活性，利用1,2-二氧杂环己烷的化学发光特性。在异源测定中，具有目的蛋白酶的切割位点的肽在一端具有第一配体结合对的第一个成员，另一端具有第二配体结合对的第一个成员。第一配体结合对的另一个成员连接到表面，其将肽或蛋白酶底物结合到表面。将肽底物与蛋白酶和目标化合物或样品组合。如果不抑制底物裂解，则可以发生底物裂解，并且去除任何未结合的切割的片段。加入与第二配体结合对的第二个成员配合的酶，并使其与残留的第二配体结合对的任何（未切割的）第一个成员结合。去除未结合的络合物，并加入酶的1,2-二氧环乙烷底物。如果任何肽底物未被切割，二氧杂环丁烷将化学发光，表明抑制活性。在同质测定中，相同的底物在一端承载荧光能量接受部分，另一端在1,2-二氧杂环丁烷或前体上。如果底物被蛋白酶切割，二氧杂环丁烷和荧光部分不是很密切的物理关系，当二氧杂环丁烷分解时，不会发生能量转移。如果没有发生裂解，表明抑制，当二氧杂环丁烷分解时，能量被转移到荧光实体，其释放与二氧杂环丁烷的波长可辨别的波长的光。

7. 发明授权

US6063574A Dioxetane labeled probes and detection assays employing the same 失效
公开(公告)号：US6063574A
公开(公告)日：2000-05-16
申请号：US18180
申请日：1998-02-03
申请人： Irena Bronstein , Brooks Edwards , Christopher Martin , John Voyta
发明人： Irena Bronstein , Brooks Edwards , Christopher Martin , John Voyta
IPC分类号： G01N33/532 , C07D321/00 , C07H21/00 , C07H21/02 , C07H21/04 , C12N15/09 , C12Q1/68 , C12N15/11
CPC分类号： C07H21/00 , C07D321/00 , C12Q1/6816 , C12Q1/686 , Y02P20/55
摘要： Probes labeled with 1,2-dioxetane precursors can be employed in a variety of assays. The probes may be nucleic acid, peptide nucleic acid, proteins including enzyme, antibody or antigen, steroid, carbohydrate, drug or non-drug hapten. The probe is provided with a 1,2-dioxetane precursor bound thereto, generally either covalently, or a strong ligand bond. The dioxetane precursor moiety is converted to a bound 1,2-dioxetane by exposure to singlet oxygen. These dioxetane (labels) either spontaneously decompose, or are induced to decompose by an appropriate trigger to release light. The trigger may be a change in pH temperature, or an agent which removes a protective group. Assay formats in which these 1,2-dioxetane labeled probes and referents may be used to include hybridization assays, immuno assays, gel-based assays and Capillary Zone Electrophoresis.

8. 发明申请

US20050026151A1 Simultaneous generation of multiple chemiluminescent signals on solid supports 审中-公开
标题翻译：在固体支持物上同时产生多种化学发光信号
公开(公告)号：US20050026151A1
公开(公告)日：2005-02-03
申请号：US10620333
申请日：2003-07-17
申请人： John Voyta , Robert Smith , Gary Schroth , Alison Sparks , Brooks Edwards
发明人： John Voyta , Robert Smith , Gary Schroth , Alison Sparks , Brooks Edwards
IPC分类号： G01N33/58 , C12Q1/68
CPC分类号： G01N33/581
摘要： A chemiluminescent assay to determine the presence and/or amount of one or more labeled target molecules in a sample is described in which the surface layer of a solid support is contacted with a composition comprising first and second chemiluminescent substrates capable of being activated by first and second enzymes, respectively. A plurality of probes are disposed on the surface layer in discrete areas. At least some of the probes are bound to a first enzyme conjugate comprising the first enzyme and at least some of the probes are bound to a second enzyme conjugate comprising the second enzyme. The resulting chemiluminescent signals are then detected. The method can be used to compare two biological samples (e.g., mRNA populations from different cells) on the same support surface or to provide a chemiluminescent control signal for normalizing chemiluminescent assay data from a biological sample.
摘要翻译：描述了用于确定样品中一种或多种标记的靶分子的存在和/或量的化学发光测定法，其中固体支持物的表面层与包含能够被第一和第二化学发光底物活化的第一和第二化学发光底物的组合物接触，第二种酶。多个探针设置在离散区域的表面层上。至少一些探针与包含第一酶的第一酶结合物结合，并且至少一些探针与包含第二酶的第二酶缀合物结合。然后检测所得到的化学发光信号。该方法可用于比较两个生物样品（例如，来自不同细胞的mRNA群体）在相同的支持物表面上，或提供化学发光控制信号，用于从生物样品中归一化化学发光测定数据。

9. 发明授权

US06451531B1 Dioxetane labeled probes and detection assays employing the same 有权
标题翻译：二氧杂环丁烷标记的探针和使用其的检测测定
公开(公告)号：US06451531B1
公开(公告)日：2002-09-17
申请号：US09340726
申请日：1999-06-29
申请人： Irena Bronstein , Brooks Edwards , Christopher Martin , John Voyta
发明人： Irena Bronstein , Brooks Edwards , Christopher Martin , John Voyta
IPC分类号： C12Q168
CPC分类号： C07H21/00 , C07D321/00 , C12Q1/6816 , C12Q1/686 , Y02P20/55 , C12Q2561/101 , C12Q2565/113
摘要： Probes labeled with 1,2-dioxetane precursors can be employed in a variety of assays. The probes may be nucleic acid, peptide nucleic acid, proteins including enzyme, antibody or antigen, steroid, carbohydrate, drug or non-drug hapten. The probe is provided with a 1,2-dioxetane precursor bound thereto, generally either covalently, or a strong ligand bond. The dioxetane precursor moiety is converted to a bound 1,2-dioxetane by exposure to singlet oxygen. These dioxetane (labels) either spontaneously decompose, or are induced to decompose by an appropriate trigger to release light. The trigger may be a change in pH temperature, or an agent which removes a protective group. Assay formats in which these 1,2-dioxetane labeled probes and referents may be used to include hybridization assays, immuno assays, gel-based assays and Capillary Zone Electrophoresis.
摘要翻译：用1,2-二氧杂环丁烷前体标记的探针可用于多种测定。探针可以是核酸，肽核酸，蛋白质，包括酶，抗体或抗原，类固醇，碳水化合物，药物或非药物半抗原。探针具有与其结合的1,2-二氧杂环丁烷前体，通常共价或强配体键。通过暴露于单线态氧将二氧杂环丁烷前体部分转化为结合的1,2-二氧环乙烷。这些二氧杂环丁烷（标记）会自发分解，或被适当的触发剂诱导分解以释放光。触发可能是pH温度的变化，或者是除去保护基团的试剂。这些1,2-二氧杂环丁烷标记的探针和指示物可用于包括杂交测定，免疫测定，基于凝胶的测定和毛细管区域电泳的测定形式。

10. 发明授权

US5336596A Membrane for chemiluminescent blotting applications 失效
标题翻译：用于化学发光印迹应用的膜
公开(公告)号：US5336596A
公开(公告)日：1994-08-09
申请号：US811620
申请日：1991-12-23
申请人： Irena Bronstein , Brooks Edwards , John Voyta
发明人： Irena Bronstein , Brooks Edwards , John Voyta
IPC分类号： G01N21/78 , B32B27/30 , C09D125/00 , C09D125/18 , G01N33/545 , G01N33/58 , C12B1/68 , B32B27/00
CPC分类号： G01N33/582 , G01N33/545 , Y10T428/31536 , Y10T428/31743 , Y10T428/31884
摘要： A new and improved polymeric membrane for use in biological assays is provided. A blotting assay employing 1,2-dioxetanes as a source of chemiluminescence employs, as an improved membrane, a polymer comprised of at least one monomer of the formula: ##STR1## The membranes reduce background signal, improve sensitivity and reliability.
摘要翻译：提供了用于生物测定的新的和改进的聚合物膜。使用1,2-二氧杂环丁烷作为化学发光源的印迹测定使用由至少一种下式的单体组成的聚合物作为改进的膜：提高了灵敏度和可靠性。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式