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1. 发明授权

US06632603B1 Non-captive substrate liquid phase immunoassay 失效
标题翻译：非捕获底物液相免疫测定
公开(公告)号：US06632603B1
公开(公告)日：2003-10-14
申请号：US08914700
申请日：1997-08-19
申请人： Thomas T. Hubscher , Erik P. Lillehoj
发明人： Thomas T. Hubscher , Erik P. Lillehoj
IPC分类号： G01N33533
CPC分类号： G01N33/585 , B01L3/5023 , B01L2300/0825 , B01L2400/0406 , G01N33/538 , G01N2333/4737 , Y10S435/971 , Y10S435/975 , Y10S436/824
摘要： An immunoassay of a liquid biological specimen for a specific analyte is conducted by forming a test sample by contacting the specimen with: (1) a binding substance of a ligand, antiligand or receptor capable of binding the analyte and (2) a detector substance of a colloidal gold or silver labeled ligand or antiligand to form a test sample. The test sample is applied by flowing onto a defined zone of an insoluble porous support film having a pore size impassable to a complex formed between the analyte, if present, with the binding substance and the detector substance, but passable to the binding substance and detector substance while remaining uncomplexed in the absence of the desired analyte. If the analyte is present in the test specimen, the detector substance binds with the analyte and the binding substance to form a visually inspectable complex on the surface of the porous support film. After application of the test sample to the porous support, the surface of the porous support is visually inspected for color to determine the presence and quantity or the absence of the analyte being assayed.
摘要翻译：对特定分析物的液体生物样品进行免疫测定，通过使样品与以下物质接触来形成测试样品：（1）能够结合分析物的配体，反配体或受体的结合物质和（2）检测物质胶体金或银标记的配体或反配体以形成测试样品。通过流动到不溶性多孔载体膜的限定区域上来施加测试样品，所述不溶性多孔载体膜的孔隙尺寸不能通过分析物（如果存在）与结合物质和检测物质形成的复合物通过，但可通过结合物质和检测器物质，同时在不存在所需分析物的情况下保持未络合。如果分析物存在于测试样品中，检测器物质与分析物和结合物质结合，以在多孔载体膜的表面上形成目视检查的复合物。在将测试样品施加到多孔载体上之后，目视检查多孔载体的表面的颜色，以确定待分析物的存在和数量或不存在。

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