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1. 发明授权

US08510053B2 Method for modifying a property of a protein 有权
标题翻译：改变蛋白质性质的方法
公开(公告)号：US08510053B2
公开(公告)日：2013-08-13
申请号：US10933280
申请日：2004-09-03
申请人： Yosuke Nishio , Eiichiro Kimura , Yoshihiro Usuda , Kazuho Ikeo , Yoji Nakamura , Takashi Gojobori , Yutaka Kawarabayashi , Yumi Hino , Eiichi Hori , Jun Yamazaki
发明人： Yosuke Nishio , Eiichiro Kimura , Yoshihiro Usuda , Kazuho Ikeo , Yoji Nakamura , Takashi Gojobori , Yutaka Kawarabayashi , Yumi Hino , Eiichi Hori , Jun Yamazaki
IPC分类号： G01N33/50
CPC分类号： C12N9/88 , C12N9/00 , C12N9/0016 , C12N9/1205 , C12N15/1034 , C12N15/1089
摘要： The present invention relates to a method for modifying a property of a protein. The present invention also relates to a method for producing a protein which has a modified property, and a method for producing a microorganism which has a modified property. The present invention is useful in the field of microbial industrial production and the like.
摘要翻译：本发明涉及一种改变蛋白质性质的方法。本发明还涉及具有改性特性的蛋白质的制造方法，以及具有改性的微生物的制造方法。本发明在微生物工业生产等领域中是有用的。

2. 发明申请

US20050233308A1 Method for modifying a property of a protein 有权
标题翻译：改变蛋白质性质的方法
公开(公告)号：US20050233308A1
公开(公告)日：2005-10-20
申请号：US10933280
申请日：2004-09-03
申请人： Yosuke Nishio , Eiichiro Kimura , Yoshihiro Usuda , Kazuho Ikeo , Yoji Nakamura , Takashi Gojobori , Yutaka Kawarabayashi , Yumi Hino , Eiichi Hori , Jun Yamazaki
发明人： Yosuke Nishio , Eiichiro Kimura , Yoshihiro Usuda , Kazuho Ikeo , Yoji Nakamura , Takashi Gojobori , Yutaka Kawarabayashi , Yumi Hino , Eiichi Hori , Jun Yamazaki
IPC分类号： C12N15/09 , C12N1/21 , C12N9/00 , C12N15/10 , C12P21/02 , C12Q1/00 , G01N33/48 , G01N33/50 , G06F19/00
CPC分类号： C12N9/88 , C12N9/00 , C12N9/0016 , C12N9/1205 , C12N15/1034 , C12N15/1089
摘要： A property of a protein is modified by the following steps: (a) selecting 1000 or more genes from the genome of a first microorganism, and selecting 1000 or more genes from the genome of a second microorganism, wherein the genes from the first microorganism are orthologs to the genes from the second microorganism, and wherein the second microorganism is closely related to the first microorganism, but grows differently under at least one optimum growth condition when compared with the first microorganism, (b) comparing an amino acid sequence encoded by a gene from the first microorganism to an amino acid sequence encoded by the orthologous gene from the second microorganism, (c) detecting substitutions between the amino acid sequence encoded by a gene from the first microorganism and the amino acid sequence encoded by a gene from the second microorganism for each pair of orthologous genes, (d) compiling the detected amino acid substitutions for each amino acid substitution type, (e) calculating the frequency of each amino acid substitution type, wherein for each detected amino acid substitution type, a correction is made by subtracting the total number of substitution types which occur from the first microorganism to the second microorganism from the total number of the same substitution type which occurs in the reverse direction, or from the second microorganism to the first microorganism, (f) identifying and labelling the amino acid substitutions which occur at a high frequency as amino acid substitutions which are involved in said optimum growth condition, and (g) introducing one or more of the amino acid substitutions identified in (f) into the gene encoding the protein to modify a property of the protein.
摘要翻译：通过以下步骤修饰蛋白质的性质：（a）从第一微生物的基因组中选择1000个或更多个基因，并从第二微生物的基因组中选择1000个或更多个基因，其中来自第一个微生物的基因是与第二微生物的基因直系同源，其中第二微生物与第一微生物密切相关，但与第一微生物相比，在至少一个最佳生长条件下生长不同，（b）比较由第一微生物编码的氨基酸序列基因从第一微生物到由第二微生物的直系同源基因编码的氨基酸序列，（c）检测由第一微生物的基因编码的氨基酸序列与由第二微生物的基因编码的氨基酸序列之间的取代每对直系同源基因的微生物，（d）编码每个氨基酸取代类型的检测到的氨基酸取代，（e）cal 规定每个氨基酸取代型的频率，其中对于每个检测到的氨基酸取代类型，通过从相同的取代类型的总数中减去从第一微生物发生到第二微生物的替代类型的总数来进行校正（f）鉴定和标记高频发生的氨基酸取代作为参与所述最佳生长条件的氨基酸取代，和（g）将（f）中鉴定的一个或多个氨基酸取代引入编码蛋白质的基因中以改变蛋白质的性质。

3. 发明授权

US5977331A .alpha.-Ketoglutarate dehydrogenase gene 失效
标题翻译： α-酮戊二酸脱氢酶基因
公开(公告)号：US5977331A
公开(公告)日：1999-11-02
申请号：US750152
申请日：1996-12-12
申请人： Yoko Asakura , Yoshihiro Usuda , Nobuharu Tsujimoto , Eiichiro Kimura , Chizu Abe , Yoshio Kawahara , Tsuyoshi Nakamatsu , Osamu Kurahashi
发明人： Yoko Asakura , Yoshihiro Usuda , Nobuharu Tsujimoto , Eiichiro Kimura , Chizu Abe , Yoshio Kawahara , Tsuyoshi Nakamatsu , Osamu Kurahashi
IPC分类号： C12N1/21 , C12N9/02 , C12N15/53 , C12P13/08 , C12P13/14 , C07H21/02 , C12N1/20 , C12P13/04
CPC分类号： C12N9/0008 , C12P13/08 , C12P13/14
摘要： Disclosed are coryneform L-glutamic acid-producing bacteria deficient in .alpha.-ketoglutarate dehydrogenase, a method of producing L-glutamic acid by using the bacteria, a gene coding for an enzyme having .alpha.-KGDH activity originating from coryneform L-glutamic acid-producing bacteria, recombinant DNA containing the gene, coryneform bacteria harboring the recombinant DNA, and a method of producing L-lysine by using bacteria harboring the recombinant DNA and having L-lysine productivity.
摘要翻译： PCT No.PCT / JP95 / 01131 Sec。 371日期：1996年12月12日 102（e）日期1996年12月12日PCT提交1995年6月7日PCT公布。 WO95 / 34672 PCT公开号日期1995年12月21日公开是α-酮戊二酸脱氢酶缺乏的棒状L-谷氨酸生产菌，使用细菌生产L-谷氨酸的方法，编码具有源自棒状杆菌L的α-KGDH活性的酶的基因谷氨酸生产菌，含有该基因的重组DNA，携带重组DNA的棒状细菌，以及通过使用含有重组DNA并具有L-赖氨酸生产力的细菌产生L-赖氨酸的方法。

4. 发明授权

US07244569B2 Plasmid autonomously replicable in coryneform bacteria 失效
标题翻译：质粒在棒状细菌中自主复制
公开(公告)号：US07244569B2
公开(公告)日：2007-07-17
申请号：US10832299
申请日：2004-04-27
申请人： Yumi Matsuzaki , Eiichiro Kimura , Tsuyoshi Nakamatsu , Osamu Kurahashi , Yoshio Kawahara , Shinichi Sugimoto
发明人： Yumi Matsuzaki , Eiichiro Kimura , Tsuyoshi Nakamatsu , Osamu Kurahashi , Yoshio Kawahara , Shinichi Sugimoto
IPC分类号： C12N15/74 , C12N15/77 , C12N15/10 , C12N15/31 , C07H21/04 , C07K14/195 , C07K14/34
CPC分类号： C12N15/77
摘要： A plasmid isolatable from Corynebacterium thermoaminogenes, which comprises a gene coding for a Rep protein having the amino acid sequence shown in SEQ ID NO: 8 or an amino acid sequence having homology of 90% or more to the amino acid sequence shown in SEQ ID NO: 8, and has a size of about 4.4 kb or about 6 kb, or a derivative thereof.
摘要翻译：可从热棒状杆菌分离的质粒，其包含编码具有SEQ ID NO：8所示氨基酸序列的Rep蛋白的基因或与SEQ ID NO所示的氨基酸序列具有90％以上同源性的氨基酸序列：8，并且具有约4.4kb或约6kb的大小，或其衍生物。

5. 发明申请

US20070031913A1 Amino-acid biosensor, fischer-ratio biosensor and health information management system 失效
标题翻译：氨基酸生物传感器，费 - 比生物传感器和健康信息管理系统
公开(公告)号：US20070031913A1
公开(公告)日：2007-02-08
申请号：US11498982
申请日：2006-08-04
申请人： Eiichiro Kimura
发明人： Eiichiro Kimura
IPC分类号： C12Q1/37
CPC分类号： C12Q1/32
摘要： Disclosed is a biosensor capable of measuring a total concentration of plural types of amino acids. An amino-acid biosensor (200) for measuring a total concentration of a plurality of specific amino acids, comprises a measuring electrode (202) which includes as components a mediator and an enzyme which selectively act on the plurality of specific amino acids each serving as a substrate, and a counter electrode (203). The enzyme has a substrate affinity to each of the plurality of specific amino acids. The enzyme is operable to catalyze a reaction in each of the plurality of specific amino acids as a substrate so as to form a reaction product, and the mediator is operable, during amino-acid concentration measurement, to carry electrons between the reaction product and the measuring electrode. The amino-acid biosensor is designed to apply a voltage between the measuring electrode and the counter electrode during the measurement in such a manner that, in an analytical curve representing a relationship between an applied voltage and a current value for each of the plurality of specific amino acids, the applied voltage includes a voltage allowing the variety of the current values for the amino acids at the same applied voltage to fall within a given range.
摘要翻译：公开了能够测定多种氨基酸的总浓度的生物传感器。用于测量多种特定氨基酸的总浓度的氨基酸生物传感器（200）包括测量电极（202），其包含作为成分的介质和选择性地作用于多个特定氨基酸的酶，每个氨基酸用作基板和对电极（203）。该酶对多种特定氨基酸中的每一种具有底物亲和力。该酶可操作地催化多种特定氨基酸中的每一种作为底物的反应以形成反应产物，并且在氨基酸浓度测量期间，介体可操作地在反应产物和测量电极。氨基酸生物传感器被设计成在测量期间在测量电极和对电极之间施加电压，使得在分析曲线中，表示施加的电压与多个特定的电极中的每一个的电流值之间的关系氨基酸，所施加的电压包括允许在相同施加电压下的氨基酸的各种电流值落在给定范围内的电压。

6. 发明授权

US06905819B1 Plasmid autonomously replicable in coryneform bacteria 失效
标题翻译：质粒在棒状细菌中自主复制
公开(公告)号：US06905819B1
公开(公告)日：2005-06-14
申请号：US09636458
申请日：2000-08-11
申请人： Yumi Matsuzaki , Eiichiro Kimura , Tsuyoshi Nakamatsu , Osamu Kurahashi , Yoshio Kawahara , Shinichi Sugimoto
发明人： Yumi Matsuzaki , Eiichiro Kimura , Tsuyoshi Nakamatsu , Osamu Kurahashi , Yoshio Kawahara , Shinichi Sugimoto
IPC分类号： C12N15/00 , C12N1/21 , C12N15/77 , C12R1/15 , C12N15/10 , C12N1/20 , C12Q1/68
CPC分类号： C12N15/77
摘要： A plasmid which is able to be isolated from Corynebacterium thermoaminogenes, and which comprises a gene coding for a Rep protein having the amino acid sequence shown in SEQ ID NO: 4 or an amino acid sequence having homology of 90% or more to the amino acid sequence shown in SEQ ID NO: 4. and has a size of about 4.4 kb or about 6 kb, or a derivative thereof.
摘要翻译：能够从嗜热棒杆菌分离的质粒，其包含编码具有SEQ ID NO：4所示的氨基酸序列的Rep蛋白的基因或与氨基酸同源性为90％以上的氨基酸序列 SEQ ID NO：4所示的序列，其大小为约4.4kb或约6kb，或其衍生物。

7. 发明授权

US08440068B2 Amino-acid biosensor, fischer-ratio biosensor and health information management system 有权
标题翻译：氨基酸生物传感器，费 - 比生物传感器和健康信息管理系统
公开(公告)号：US08440068B2
公开(公告)日：2013-05-14
申请号：US12585638
申请日：2009-09-21
申请人： Eiichiro Kimura
发明人： Eiichiro Kimura
IPC分类号： G01N27/26 , C12Q1/32
CPC分类号： C12Q1/32
摘要： Disclosed is a biosensor capable of measuring a total concentration of plural types of amino acids. An amino-acid biosensor (200) for measuring a total concentration of a plurality of specific amino acids, comprises a measuring electrode (202) which includes as components a mediator and an enzyme which selectively act on the plurality of specific amino acids each serving as a substrate, and a counter electrode (203). The enzyme has a substrate affinity to each of the plurality of specific amino acids. The enzyme is operable to catalyze a reaction in each of the plurality of specific amino acids as a substrate so as to form a reaction product, and the mediator is operable, during amino-acid concentration measurement, to carry electrons between the reaction product and the measuring electrode. The amino-acid biosensor is designed to apply a voltage between the measuring electrode and the counter electrode during the measurement in such a manner that, in an analytical curve representing a relationship between an applied voltage and a current value for each of the plurality of specific amino acids, the applied voltage includes a voltage allowing the variety of the current values for the amino acids at the same applied voltage to fall within a given range.
摘要翻译：公开了能够测定多种氨基酸的总浓度的生物传感器。用于测量多种特定氨基酸的总浓度的氨基酸生物传感器（200）包括测量电极（202），其包含作为成分的介质和选择性地作用于多个特定氨基酸的酶，每个氨基酸用作基板和对电极（203）。该酶对多种特定氨基酸中的每一种具有底物亲和力。该酶可操作地催化多种特定氨基酸中的每一种作为底物的反应以形成反应产物，并且在氨基酸浓度测量期间，介体可操作地在反应产物和测量电极。氨基酸生物传感器被设计成在测量期间在测量电极和对电极之间施加电压，使得在分析曲线中，表示施加的电压与多个特定的电极中的每一个的电流值之间的关系氨基酸，所施加的电压包括允许在相同施加电压下的氨基酸的各种电流值落在给定范围内的电压。

8. 发明申请

US20120077708A1 BETA-2 MICROGLOBULIN AS A BIOMARKER FOR PERIPHERAL ARTERY DISEASE 失效
标题翻译： BETA-2 MICROGLOBULIN作为外周动脉疾病的生物标志物
公开(公告)号：US20120077708A1
公开(公告)日：2012-03-29
申请号：US13243593
申请日：2011-09-23
申请人： John Cooke , Eric T. Fung , Eiichiro Kimura
发明人： John Cooke , Eric T. Fung , Eiichiro Kimura
IPC分类号： C40B30/10 , G01N33/566 , G01N33/573
CPC分类号： G01N33/564 , G01N33/6893 , G01N2800/323 , G01N2800/52
摘要： The present invention provides β2 microglobulin as a biomarker for qualifying or assessing peripheral artery disease in a subject.
摘要翻译：本发明提供了一种2微球蛋白作为用于在受试者中鉴定或评估外周动脉疾病的生物标志物。

9. 发明申请

US20110151481A1 BETA-2 MICROGLOBULIN AS A BIOMARKER FOR PERIPHERAL ARTERY DISEASE 失效
标题翻译： BETA-2 MICROGLOBULIN作为外周动脉疾病的生物标志物
公开(公告)号：US20110151481A1
公开(公告)日：2011-06-23
申请号：US12972867
申请日：2010-12-20
申请人： John Cooke , Eric T. Fung , Eiichiro Kimura
发明人： John Cooke , Eric T. Fung , Eiichiro Kimura
IPC分类号： G01N33/573 , G01N33/543 , G01N33/68 , G01N33/92 , G06F19/00 , A61B5/00
CPC分类号： G01N33/564 , G01N33/6893 , G01N2800/323 , G01N2800/52
摘要： The present invention provides β2 microglobulin as a biomarker for qualifying or assessing peripheral artery disease in a subject.
摘要翻译：本发明提供了一种2微球蛋白作为用于在受试者中鉴定或评估外周动脉疾病的生物标志物。

10. 发明申请

US20100017147A1 Amino-acid biosensor, Fischer-ratio biosensor and health information management system 有权
公开(公告)号：US20100017147A1
公开(公告)日：2010-01-21
申请号：US12585638
申请日：2009-09-21
申请人： Eiichiro Kimura
发明人： Eiichiro Kimura
IPC分类号： G01N33/48
CPC分类号： C12Q1/32
摘要： Disclosed is a biosensor capable of measuring a total concentration of plural types of amino acids. An amino-acid biosensor (200) for measuring a total concentration of a plurality of specific amino acids, comprises a measuring electrode (202) which includes as components a mediator and an enzyme which selectively act on the plurality of specific amino acids each serving as a substrate, and a counter electrode (203). The enzyme has a substrate affinity to each of the plurality of specific amino acids. The enzyme is operable to catalyze a reaction in each of the plurality of specific amino acids as a substrate so as to form a reaction product, and the mediator is operable, during amino-acid concentration measurement, to carry electrons between the reaction product and the measuring electrode. The amino-acid biosensor is designed to apply a voltage between the measuring electrode and the counter electrode during the measurement in such a manner that, in an analytical curve representing a relationship between an applied voltage and a current value for each of the plurality of specific amino acids, the applied voltage includes a voltage allowing the variety of the current values for the amino acids at the same applied voltage to fall within a given range.

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