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1. 发明授权

US08440068B2 Amino-acid biosensor, fischer-ratio biosensor and health information management system 有权
标题翻译：氨基酸生物传感器，费 - 比生物传感器和健康信息管理系统
公开(公告)号：US08440068B2
公开(公告)日：2013-05-14
申请号：US12585638
申请日：2009-09-21
申请人： Eiichiro Kimura
发明人： Eiichiro Kimura
IPC分类号： G01N27/26 , C12Q1/32
CPC分类号： C12Q1/32
摘要： Disclosed is a biosensor capable of measuring a total concentration of plural types of amino acids. An amino-acid biosensor (200) for measuring a total concentration of a plurality of specific amino acids, comprises a measuring electrode (202) which includes as components a mediator and an enzyme which selectively act on the plurality of specific amino acids each serving as a substrate, and a counter electrode (203). The enzyme has a substrate affinity to each of the plurality of specific amino acids. The enzyme is operable to catalyze a reaction in each of the plurality of specific amino acids as a substrate so as to form a reaction product, and the mediator is operable, during amino-acid concentration measurement, to carry electrons between the reaction product and the measuring electrode. The amino-acid biosensor is designed to apply a voltage between the measuring electrode and the counter electrode during the measurement in such a manner that, in an analytical curve representing a relationship between an applied voltage and a current value for each of the plurality of specific amino acids, the applied voltage includes a voltage allowing the variety of the current values for the amino acids at the same applied voltage to fall within a given range.
摘要翻译：公开了能够测定多种氨基酸的总浓度的生物传感器。用于测量多种特定氨基酸的总浓度的氨基酸生物传感器（200）包括测量电极（202），其包含作为成分的介质和选择性地作用于多个特定氨基酸的酶，每个氨基酸用作基板和对电极（203）。该酶对多种特定氨基酸中的每一种具有底物亲和力。该酶可操作地催化多种特定氨基酸中的每一种作为底物的反应以形成反应产物，并且在氨基酸浓度测量期间，介体可操作地在反应产物和测量电极。氨基酸生物传感器被设计成在测量期间在测量电极和对电极之间施加电压，使得在分析曲线中，表示施加的电压与多个特定的电极中的每一个的电流值之间的关系氨基酸，所施加的电压包括允许在相同施加电压下的氨基酸的各种电流值落在给定范围内的电压。

2. 发明申请

US20120077708A1 BETA-2 MICROGLOBULIN AS A BIOMARKER FOR PERIPHERAL ARTERY DISEASE 失效
标题翻译： BETA-2 MICROGLOBULIN作为外周动脉疾病的生物标志物
公开(公告)号：US20120077708A1
公开(公告)日：2012-03-29
申请号：US13243593
申请日：2011-09-23
申请人： John Cooke , Eric T. Fung , Eiichiro Kimura
发明人： John Cooke , Eric T. Fung , Eiichiro Kimura
IPC分类号： C40B30/10 , G01N33/566 , G01N33/573
CPC分类号： G01N33/564 , G01N33/6893 , G01N2800/323 , G01N2800/52
摘要： The present invention provides β2 microglobulin as a biomarker for qualifying or assessing peripheral artery disease in a subject.
摘要翻译：本发明提供了一种2微球蛋白作为用于在受试者中鉴定或评估外周动脉疾病的生物标志物。

3. 发明申请

US20110151481A1 BETA-2 MICROGLOBULIN AS A BIOMARKER FOR PERIPHERAL ARTERY DISEASE 失效
标题翻译： BETA-2 MICROGLOBULIN作为外周动脉疾病的生物标志物
公开(公告)号：US20110151481A1
公开(公告)日：2011-06-23
申请号：US12972867
申请日：2010-12-20
申请人： John Cooke , Eric T. Fung , Eiichiro Kimura
发明人： John Cooke , Eric T. Fung , Eiichiro Kimura
IPC分类号： G01N33/573 , G01N33/543 , G01N33/68 , G01N33/92 , G06F19/00 , A61B5/00
CPC分类号： G01N33/564 , G01N33/6893 , G01N2800/323 , G01N2800/52
摘要： The present invention provides β2 microglobulin as a biomarker for qualifying or assessing peripheral artery disease in a subject.
摘要翻译：本发明提供了一种2微球蛋白作为用于在受试者中鉴定或评估外周动脉疾病的生物标志物。

4. 发明申请

US20100017147A1 Amino-acid biosensor, Fischer-ratio biosensor and health information management system 有权
公开(公告)号：US20100017147A1
公开(公告)日：2010-01-21
申请号：US12585638
申请日：2009-09-21
申请人： Eiichiro Kimura
发明人： Eiichiro Kimura
IPC分类号： G01N33/48
CPC分类号： C12Q1/32
摘要： Disclosed is a biosensor capable of measuring a total concentration of plural types of amino acids. An amino-acid biosensor (200) for measuring a total concentration of a plurality of specific amino acids, comprises a measuring electrode (202) which includes as components a mediator and an enzyme which selectively act on the plurality of specific amino acids each serving as a substrate, and a counter electrode (203). The enzyme has a substrate affinity to each of the plurality of specific amino acids. The enzyme is operable to catalyze a reaction in each of the plurality of specific amino acids as a substrate so as to form a reaction product, and the mediator is operable, during amino-acid concentration measurement, to carry electrons between the reaction product and the measuring electrode. The amino-acid biosensor is designed to apply a voltage between the measuring electrode and the counter electrode during the measurement in such a manner that, in an analytical curve representing a relationship between an applied voltage and a current value for each of the plurality of specific amino acids, the applied voltage includes a voltage allowing the variety of the current values for the amino acids at the same applied voltage to fall within a given range.

5. 发明授权

US07608437B2 Method of constructing amino acid producing bacterial strains, and method of preparing amino acids by fermentation with the constructed amino acid producing bacterial strains 有权
标题翻译：构建产氨酸的细菌菌株的方法，以及用构建的产生氨基酸的菌株通过发酵制备氨基酸的方法
公开(公告)号：US07608437B2
公开(公告)日：2009-10-27
申请号：US11218433
申请日：2005-09-06
申请人： Yoko Asakura , Jun Nakamura , Sohei Kanno , Mikiko Suga , Eiichiro Kimura , Hisao Ito , Kazuhiko Matsui , Tsuyoshi Ohsumi , Tsuyoshi Nakamatsu , Osamu Kurahashi
发明人： Yoko Asakura , Jun Nakamura , Sohei Kanno , Mikiko Suga , Eiichiro Kimura , Hisao Ito , Kazuhiko Matsui , Tsuyoshi Ohsumi , Tsuyoshi Nakamatsu , Osamu Kurahashi
IPC分类号： C12P13/14 , C12Q1/48 , C12Q1/32 , C12Q1/68 , C12N9/02 , C12N9/10 , C12N1/21 , C12P21/00 , C12N15/00 , C07K14/00 , C07H21/00
CPC分类号： C12P13/14 , C12N15/67
摘要： A method of producing coryneform bacteria having an improved amino acid or nucleic acid-productivity comprises the steps of introducing a mutation in a promoter sequence of amino acid- or nucleic acid-biosynthesizing genes on a chromosome of a coryneform bacterium so that it is close to a consensus sequence or introducing a change in the promoter sequence of amino acid- or nucleic acid-biosynthesizing genes on the chromosome of a coryneform bacterium by gene recombination so that it is close to a consensus sequence, obtaining mutants of the coryneform amino acid- or nucleic acid-producing microorganism, culturing the mutants and select a mutant capable of producing the intended amino acid or nucleic acid in a large amount. This method allows one of skill in the art to construct a mutant capable of enriching or controlling the expression of an intended gene without using a plasmid and to promote production of amino acids in a high yield, by the recombination or mutation.
摘要翻译：具有改进的氨基酸或核酸生产力的棒状杆菌细菌的方法包括以下步骤：将氨基酸或核酸 - 生物合成基因的启动子序列中的突变引入棒状细菌的染色体上，使其接近共有序列或通过基因重组在棒状细菌的染色体上引入氨基酸或核酸 - 生物合成基因的启动子序列的变化，使得其接近共有序列，获得棒状杆菌型氨基酸的突变体或产生核酸的微生物，培养突变体并选择能够大量产生所需氨基酸或核酸的突变体。该方法允许本领域技术人员通过重组或突变来构建能够富集或控制预期基因的表达而不使用质粒并以高产率促进氨基酸产生的突变体。

6. 发明授权

US06338956B1 Stress-resistant microorganism and method of producing fermentative product 有权
标题翻译：耐应力微生物和发酵产物的生产方法
公开(公告)号：US06338956B1
公开(公告)日：2002-01-15
申请号：US09634632
申请日：2000-08-08
申请人： Eiichiro Kimura , Yoshimi Kikuchi , Yoshio Kawahara , Shinya Goto , Osamu Kurahashi , Tsuyoshi Nakamatsu
发明人： Eiichiro Kimura , Yoshimi Kikuchi , Yoshio Kawahara , Shinya Goto , Osamu Kurahashi , Tsuyoshi Nakamatsu
IPC分类号： C12P1304
CPC分类号： C12P13/222 , C12P13/04 , C12P13/08 , C12P13/14
摘要： A microorganism is utilized to fermentatively produce useful substances such as amino acids by cultivating the microorganism in a medium to allow a fermentative product to be produced and accumulated in the medium, and collecting the fermentative product, wherein the microorganism to be used is modified by introduction of at least one of a gene coding for a heat shock protein and a gene coding for a &sgr; factor which specifically functions for the heat shock protein gene to enhance expression amount of the heat shock protein in cells, whereby the microorganism is allowed to have added resistance to stress which would otherwise restrain growth of the microorganism and/or production of the fermentative product.
摘要翻译：利用微生物通过在培养基中培养微生物发酵生产有用物质如氨基酸，使发酵产物在培养基中产生和积累，收集发酵产物，其中所用的微生物通过引入进行修饰编码热休克蛋白的基因中的至少一种和编码特异性用于热休克蛋白基因的西格玛因子的基因以增强细胞中的热休克蛋白的表达量，由此允许微生物添加否则会抑制微生物的生长和/或发酵产物的产生。

7. 发明授权

US5977331A .alpha.-Ketoglutarate dehydrogenase gene 失效
标题翻译： α-酮戊二酸脱氢酶基因
公开(公告)号：US5977331A
公开(公告)日：1999-11-02
申请号：US750152
申请日：1996-12-12
申请人： Yoko Asakura , Yoshihiro Usuda , Nobuharu Tsujimoto , Eiichiro Kimura , Chizu Abe , Yoshio Kawahara , Tsuyoshi Nakamatsu , Osamu Kurahashi
发明人： Yoko Asakura , Yoshihiro Usuda , Nobuharu Tsujimoto , Eiichiro Kimura , Chizu Abe , Yoshio Kawahara , Tsuyoshi Nakamatsu , Osamu Kurahashi
IPC分类号： C12N1/21 , C12N9/02 , C12N15/53 , C12P13/08 , C12P13/14 , C07H21/02 , C12N1/20 , C12P13/04
CPC分类号： C12N9/0008 , C12P13/08 , C12P13/14
摘要： Disclosed are coryneform L-glutamic acid-producing bacteria deficient in .alpha.-ketoglutarate dehydrogenase, a method of producing L-glutamic acid by using the bacteria, a gene coding for an enzyme having .alpha.-KGDH activity originating from coryneform L-glutamic acid-producing bacteria, recombinant DNA containing the gene, coryneform bacteria harboring the recombinant DNA, and a method of producing L-lysine by using bacteria harboring the recombinant DNA and having L-lysine productivity.
摘要翻译： PCT No.PCT / JP95 / 01131 Sec。 371日期：1996年12月12日 102（e）日期1996年12月12日PCT提交1995年6月7日PCT公布。 WO95 / 34672 PCT公开号日期1995年12月21日公开是α-酮戊二酸脱氢酶缺乏的棒状L-谷氨酸生产菌，使用细菌生产L-谷氨酸的方法，编码具有源自棒状杆菌L的α-KGDH活性的酶的基因谷氨酸生产菌，含有该基因的重组DNA，携带重组DNA的棒状细菌，以及通过使用含有重组DNA并具有L-赖氨酸生产力的细菌产生L-赖氨酸的方法。

8. 发明授权

US07244569B2 Plasmid autonomously replicable in coryneform bacteria 失效
标题翻译：质粒在棒状细菌中自主复制
公开(公告)号：US07244569B2
公开(公告)日：2007-07-17
申请号：US10832299
申请日：2004-04-27
申请人： Yumi Matsuzaki , Eiichiro Kimura , Tsuyoshi Nakamatsu , Osamu Kurahashi , Yoshio Kawahara , Shinichi Sugimoto
发明人： Yumi Matsuzaki , Eiichiro Kimura , Tsuyoshi Nakamatsu , Osamu Kurahashi , Yoshio Kawahara , Shinichi Sugimoto
IPC分类号： C12N15/74 , C12N15/77 , C12N15/10 , C12N15/31 , C07H21/04 , C07K14/195 , C07K14/34
CPC分类号： C12N15/77
摘要： A plasmid isolatable from Corynebacterium thermoaminogenes, which comprises a gene coding for a Rep protein having the amino acid sequence shown in SEQ ID NO: 8 or an amino acid sequence having homology of 90% or more to the amino acid sequence shown in SEQ ID NO: 8, and has a size of about 4.4 kb or about 6 kb, or a derivative thereof.
摘要翻译：可从热棒状杆菌分离的质粒，其包含编码具有SEQ ID NO：8所示氨基酸序列的Rep蛋白的基因或与SEQ ID NO所示的氨基酸序列具有90％以上同源性的氨基酸序列：8，并且具有约4.4kb或约6kb的大小，或其衍生物。

9. 发明申请

US20070031913A1 Amino-acid biosensor, fischer-ratio biosensor and health information management system 失效
标题翻译：氨基酸生物传感器，费 - 比生物传感器和健康信息管理系统
公开(公告)号：US20070031913A1
公开(公告)日：2007-02-08
申请号：US11498982
申请日：2006-08-04
申请人： Eiichiro Kimura
发明人： Eiichiro Kimura
IPC分类号： C12Q1/37
CPC分类号： C12Q1/32
摘要： Disclosed is a biosensor capable of measuring a total concentration of plural types of amino acids. An amino-acid biosensor (200) for measuring a total concentration of a plurality of specific amino acids, comprises a measuring electrode (202) which includes as components a mediator and an enzyme which selectively act on the plurality of specific amino acids each serving as a substrate, and a counter electrode (203). The enzyme has a substrate affinity to each of the plurality of specific amino acids. The enzyme is operable to catalyze a reaction in each of the plurality of specific amino acids as a substrate so as to form a reaction product, and the mediator is operable, during amino-acid concentration measurement, to carry electrons between the reaction product and the measuring electrode. The amino-acid biosensor is designed to apply a voltage between the measuring electrode and the counter electrode during the measurement in such a manner that, in an analytical curve representing a relationship between an applied voltage and a current value for each of the plurality of specific amino acids, the applied voltage includes a voltage allowing the variety of the current values for the amino acids at the same applied voltage to fall within a given range.
摘要翻译：公开了能够测定多种氨基酸的总浓度的生物传感器。用于测量多种特定氨基酸的总浓度的氨基酸生物传感器（200）包括测量电极（202），其包含作为成分的介质和选择性地作用于多个特定氨基酸的酶，每个氨基酸用作基板和对电极（203）。该酶对多种特定氨基酸中的每一种具有底物亲和力。该酶可操作地催化多种特定氨基酸中的每一种作为底物的反应以形成反应产物，并且在氨基酸浓度测量期间，介体可操作地在反应产物和测量电极。氨基酸生物传感器被设计成在测量期间在测量电极和对电极之间施加电压，使得在分析曲线中，表示施加的电压与多个特定的电极中的每一个的电流值之间的关系氨基酸，所施加的电压包括允许在相同施加电压下的氨基酸的各种电流值落在给定范围内的电压。

10. 发明授权

US06905819B1 Plasmid autonomously replicable in coryneform bacteria 失效
标题翻译：质粒在棒状细菌中自主复制
公开(公告)号：US06905819B1
公开(公告)日：2005-06-14
申请号：US09636458
申请日：2000-08-11
申请人： Yumi Matsuzaki , Eiichiro Kimura , Tsuyoshi Nakamatsu , Osamu Kurahashi , Yoshio Kawahara , Shinichi Sugimoto
发明人： Yumi Matsuzaki , Eiichiro Kimura , Tsuyoshi Nakamatsu , Osamu Kurahashi , Yoshio Kawahara , Shinichi Sugimoto
IPC分类号： C12N15/00 , C12N1/21 , C12N15/77 , C12R1/15 , C12N15/10 , C12N1/20 , C12Q1/68
CPC分类号： C12N15/77
摘要： A plasmid which is able to be isolated from Corynebacterium thermoaminogenes, and which comprises a gene coding for a Rep protein having the amino acid sequence shown in SEQ ID NO: 4 or an amino acid sequence having homology of 90% or more to the amino acid sequence shown in SEQ ID NO: 4. and has a size of about 4.4 kb or about 6 kb, or a derivative thereof.
摘要翻译：能够从嗜热棒杆菌分离的质粒，其包含编码具有SEQ ID NO：4所示的氨基酸序列的Rep蛋白的基因或与氨基酸同源性为90％以上的氨基酸序列 SEQ ID NO：4所示的序列，其大小为约4.4kb或约6kb，或其衍生物。

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