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1. 发明申请

US20100124777A1 Bacterium producing L-glutamic acid and method for producing L-glutamic acid 审中-公开
标题翻译：产生L-谷氨酸的细菌和L-谷氨酸的生产方法
公开(公告)号：US20100124777A1
公开(公告)日：2010-05-20
申请号：US11785643
申请日：2007-04-19
申请人： Hiromi Ohtaki , Jun Nakamura , Hiroshi Izui , Tsuyoshi Nakamatsu
发明人： Hiromi Ohtaki , Jun Nakamura , Hiroshi Izui , Tsuyoshi Nakamatsu
IPC分类号： C12N1/21 , C07H21/04
CPC分类号： C12N9/1051 , C12N9/90 , C12P13/14 , C12R1/13 , C12R1/15
摘要： L-Glutamic acid is produced by culturing a coryneform bacterium having L-glutamic acid producing ability, in which trehalose synthesis ability is decreased or deleted by, for example, disrupting a gene coding for trehalose-6-phosphate synthase, a gene coding for maltooligosyltrehalose synthase, or both of these genes to produce and accumulate L-glutamic acid in the medium, and collecting the L-glutamic acid from the medium.
摘要翻译： L-谷氨酸是通过培养具有L-谷氨酸生产能力的棒状细菌产生的，其中海藻糖合成能力通过例如破坏编码海藻糖-6-磷酸合酶的基因减少或缺失，编码麦芽糖糖基海藻糖的基因合成酶或这两种基因，以在培养基中产生和积累L-谷氨酸，并从培养基中收集L-谷氨酸。

2. 发明授权

US07432085B2 Method for producing L-amino acid 有权
标题翻译：生产L-氨基酸的方法
公开(公告)号：US07432085B2
公开(公告)日：2008-10-07
申请号：US11281495
申请日：2005-11-18
申请人： Yoshihiko Hara , Hiroshi Izui , Takahiro Asano , Yasuyuki Watanabe , Tsuyoshi Nakamatsu
发明人： Yoshihiko Hara , Hiroshi Izui , Takahiro Asano , Yasuyuki Watanabe , Tsuyoshi Nakamatsu
IPC分类号： C12P13/04 , C12P13/06 , C12P13/16 , C12P13/20 , C12P13/24
CPC分类号： C12P13/04 , C12N9/88 , C12P13/14
摘要： In a method for producing an L-amino acid by culturing a microorganism having an ability to produce an L-amino acid in a medium to produce and accumulate the L-amino acid in the medium and collecting the L-amino acid from the medium, a Gram-negative bacterium having the Entner-Doudoroff pathway and modified so that 6-phosphogluconate dehydratase activity or 2-keto-3-deoxy-6-phosphogluconate aldolase activity, or activities of the both are enhanced is used as the microorganism.
摘要翻译：通过培养具有在培养基中产生L-氨基酸的能力的微生物来生产L-氨基酸的方法，以在培养基中产生和积累L-氨基酸并从培养基中收集L-氨基酸，具有Entner-Doudoroff途径并被修饰的革兰氏阴性细菌被用作微生物，使得6-磷酸葡萄糖酸脱水酶活性或2-酮-3-脱氧-6-磷酸葡萄糖酸醛缩酶活性或两者的活性增强。

3. 发明授权

US07351571B2 Process for the production of β-amino acids using acylase 有权
标题翻译：使用酰基转移酶生产β-氨基酸的方法
公开(公告)号：US07351571B2
公开(公告)日：2008-04-01
申请号：US11198242
申请日：2005-08-08
申请人： Tsuyoshi Nakamatsu , Hisashi Kawasaki , Kunihiko Watanabe , Masakazu Nakazawa , Kunisuke Izawa
发明人： Tsuyoshi Nakamatsu , Hisashi Kawasaki , Kunihiko Watanabe , Masakazu Nakazawa , Kunisuke Izawa
IPC分类号： C12N1/20 , C12P13/04 , C07H21/04
CPC分类号： C12P13/04 , C12P41/006 , C12R1/01
摘要： The present invention relates to a Variovorax sp. which produces an acylase having an asymmetric hydrolysis activity on an N-acetyl β-amino acid to selectively produce an R-β-amino acid, and a Burkholderia sp. which produces both an acylase having an asymmetric hydrolysis activity on an N-acetyl β-amino acid to selectively produce an S-β-amino acid and an acylase having an asymmetric hydrolysis activity of an N-acetyl β-amino acid to selectively produce an R-β-amino acid, and a process for the selective production of an S-, or R-β-amino acid using the above strains.
摘要翻译：本发明涉及一种Variovorax sp。其产生对N-乙酰基β-氨基酸具有不对称水解活性以选择性地产生R-β-氨基酸的酰基转移酶和伯克霍尔德氏菌属（Burkholderia sp。其产生对N-乙酰基β-氨基酸具有不对称水解活性的酰基转移酶以选择性地产生具有N-乙酰基β-氨基酸的不对称水解活性的S-β-氨基酸和酰基转移酶，以选择性地产生 R-β-氨基酸，以及使用上述菌株选择性生产S-或R-β-氨基酸的方法。

4. 发明授权

US07297521B2 Carbamoyl-phosphate synthetase gene of coryneform bacteria and method for producing L-arginine 有权
标题翻译：棒状细菌的氨基甲酰磷酸合成酶基因及L-精氨酸的制备方法
公开(公告)号：US07297521B2
公开(公告)日：2007-11-20
申请号：US11011701
申请日：2004-12-15
申请人： Yoko Kuwabara , Kenichi Hashiguchi , Tsuyoshi Nakamatsu , Osamu Kurahashi , Yukiko Mori , Hisao Ito
发明人： Yoko Kuwabara , Kenichi Hashiguchi , Tsuyoshi Nakamatsu , Osamu Kurahashi , Yukiko Mori , Hisao Ito
IPC分类号： C12P13/10
CPC分类号： C12N9/93 , C12P13/10
摘要： A DNA fragment which encodes a polypeptide defined in the following (a) or (b), and a polypeptide defined in the following (c) or (d): (a) a polypeptide which has at least the amino acid sequence of the amino acid numbers 50 to 393 in SEQ ID NO: 2 shown in Sequence Listing, (b) a polypeptide which has at least the amino acid sequence of the amino acid numbers 50 to 393 in SEQ ID NO: 2 shown in Sequence Listing including substitution, deletion, insertion, addition, or inversion of one or several amino acids, and can constitute a protein having a carbamoyl-phosphate synthetase activity with a large subunit of carbamoyl-phosphate synthetase having the amino acid sequence of SEQ ID NO: 3, (c) a polypeptide which has the amino acid sequence of SEQ ID NO: 3 shown in Sequence Listing, (d) a polypeptide which has the amino acid sequence of SEQ ID NO: 3 shown in Sequence Listing including substitution, deletion, insertion, addition, or inversion of one or several amino acids, and can constitute a protein having a carbamoyl-phosphate synthetase activity with a small subunit of carbamoyl-phosphate synthetase having the amino acid sequence of the amino acid numbers 50 to 393 in SEQ ID NO: 2.
摘要翻译：编码以下（a）或（b）中定义的多肽的DNA片段和以下（c）或（d）中定义的多肽：（a）至少具有氨基酸的氨基酸序列的多肽序列表所示的SEQ ID NO：2中的50〜393的酸值，（b）至少具有序列表所示的序列号2所示的氨基酸序列号为50〜393的氨基酸序列的多肽，包括取代，一个或几个氨基酸的缺失，插入，添加或倒位，并且可以构成具有氨基甲酰磷酸合成酶活性的蛋白质，其具有氨基酸序列SEQ ID NO：3的氨基甲酰磷酸合成酶的大亚基，（c ）具有序列表所示的SEQ ID NO：3的氨基酸序列的多肽，（d）具有序列表所示的SEQ ID NO：3的氨基酸序列的多肽，包括取代，缺失，插入，添加，或反转一个或几个氨基酸，并可构成具有氨基酸 - 磷酸合成酶活性的蛋白质，其具有SEQ ID NO：2中氨基酸序列号为50〜393的氨基酸序列的氨基甲酰磷酸合成酶的小亚基。

5. 发明授权

US07183403B2 Genes for heat resistant enzymes of amino acid biosynthetic pathway derived from thermophilic coryneform bacteria 有权
标题翻译：来自嗜热棒状杆菌的氨基酸生物合成途径耐热酶基因
公开(公告)号：US07183403B2
公开(公告)日：2007-02-27
申请号：US11073560
申请日：2005-03-08
申请人： Seiko Hirano , Eiichiro Kimura , Tsuyoshi Osumi , Kazuhiko Matsui , Yoshio Kawahara , Gen Nonaka , Yumi Matsuzaki , Naoki Akiyoshi , Kanae Nakamura , Osamu Kurahashi , Tsuyoshi Nakamatsu , Shinichi Sugimoto
发明人： Seiko Hirano , Eiichiro Kimura , Tsuyoshi Osumi , Kazuhiko Matsui , Yoshio Kawahara , Gen Nonaka , Yumi Matsuzaki , Naoki Akiyoshi , Kanae Nakamura , Osamu Kurahashi , Tsuyoshi Nakamatsu , Shinichi Sugimoto
IPC分类号： C07H21/04 , C12P21/06
CPC分类号： C12Y108/01004 , C07K14/34 , C12N9/0006 , C12N9/0008 , C12N9/0051 , C12N9/1205 , C12N9/2408 , C12N9/88 , C12Y101/01041 , C12Y102/01051 , C12Y102/04002 , C12Y104/01004 , C12Y203/03001 , C12Y207/01011 , C12Y302/01026 , C12Y401/01031 , C12Y401/03001 , C12Y402/01003 , C12Y604/01001 , C12Y604/01002
摘要： A plurality of primer sets are designed based on a region where conservation at the amino acid level is observed among various microorganisms for known gene sequences corresponding to a gene coding for an enzyme of the L-amino acid biosynthetic pathway derived from Corynebacterium thermoaminogenes, preferably an enzyme that functions at a higher temperature compared with that of Corynebacterium glutamicum. PCR is performed by using the primers and chromosomal DNA of Corynebacterium thermoaminogenes as a template. The primers with which an amplification fragment has been obtained are used as primers for screening to select a clone containing a target DNA fragment from a plasmid library of chromosomal DNA of Corynebacterium thermoaminogenes.
摘要翻译：基于对应于编码源自热棒状棒杆菌的L-氨基酸生物合成途径的酶的基因的已知基因序列的各种微生物，观察到在氨基酸水平上保持的区域的多个引物组，优选为与谷氨酸棒杆菌相比在更高的温度下起作用的酶。通过使用热源性棒状杆菌的引物和染色体DNA作为模板进行PCR。使用已经获得扩增片段的引物作为引物用于筛选，从嗜热氨基酸棒杆菌的染色体DNA的质粒文库中选择含有靶DNA片段的克隆。

6. 发明授权

US06881566B2 Carbamoyl-phosphate synthetase gene of coryneform bacteria and method for producing L-arginine 有权
标题翻译：棒状细菌的氨基甲酰磷酸合成酶基因及L-精氨酸的制备方法
公开(公告)号：US06881566B2
公开(公告)日：2005-04-19
申请号：US09836470
申请日：2001-04-18
申请人： Yoko Kuwabara , Kenichi Hashiguchi , Tsuyoshi Nakamatsu , Osamu Kurahashi , Yukiko Mori , Hisao Ito
发明人： Yoko Kuwabara , Kenichi Hashiguchi , Tsuyoshi Nakamatsu , Osamu Kurahashi , Yukiko Mori , Hisao Ito
IPC分类号： C12N9/00 , C12P13/10 , C12N1/20 , C07H21/04
CPC分类号： C12P13/10 , C12N9/93
摘要： A DNA fragment which encodes a polypeptide defined in the following (a) or (b), and a polypeptide defined in the following (c) or (d): (a) a polypeptide which has at least the amino acid sequence of the amino acid numbers 50 to 393 in SEQ ID NO: 2 shown in Sequence Listing, (b) a polypeptide which has at least the amino acid sequence of the amino acid numbers 50 to 393 in SEQ ID No: 2 shown in Sequence Listing including substitution, deletion, insertion, addition, or inversion of one or several amino acids, and can constitute a protein having a carbamoyl-phosphate synthetase activity with a large subunit of carbamoyl-phosphate synthetase having the amino acid sequence comprising at least the amino acid numbers 55 to 1113 of SEQ ID NO: 3, (c) a polypeptide which has the amino acid sequence comprising at least the amino acid numbers 55 to 1113 of SEQ ID NO: 3 shown in Sequence Listing, (d) a polypeptide which has the amino acid sequence comprising at least the amino acid numbers 55 to 1113 of SEQ ID NO: 3 shown in Sequence Listing including substitution, deletion, insertion, addition, or inversion of one or several amino acids, and can constitute a protein having a carbamoyl-phosphate synthetase activity with a small subunit of carbamoyl-phosphate synthetase having the amino acid sequence of the amino acid numbers 50 to 393 in SEQ ID NO: 2.
摘要翻译：编码以下（a）或（b）中定义的多肽的DNA片段和以下（c）或（d）中定义的多肽：（a）至少具有氨基酸的氨基酸序列的多肽序列表所示的SEQ ID NO：2中的酸值为50〜393，（b）至少具有序列表所示的SEQ ID No：2的氨基酸序列号为50〜393的氨基酸序列的多肽，包括取代，一个或几个氨基酸的缺失，插入，添加或倒位，并且可以构成具有氨基甲酰基 - 磷酸合成酶活性的蛋白质，其具有氨基酸 - 磷酸合成酶的大亚基，其氨基酸序列至少包含氨基酸数55至 SEQ ID NO：3的1113，（c）具有至少包含序列表所示的SEQ ID NO：3的氨基酸编号55至1113的氨基酸序列的多肽，（d）具有氨基酸的多肽序列至少包含氨基酸编号55至11 包括序列表中所示的SEQ ID NO：3的13个氨基酸残基，其包含一个或多个氨基酸的取代，缺失，插入，添加或倒位，并且可以构成具有氨甲酰磷酸合成酶活性的氨基甲酰磷酸合成酶的小亚基具有SEQ ID NO：2中氨基酸序列号50至393的氨基酸序列。

7. 发明授权

US07262035B2 Method for producing L-glutamine by fermentation and L-glutamine producing bacterium 有权
标题翻译：通过发酵和L-谷氨酰胺生产菌生产L-谷氨酰胺的方法
公开(公告)号：US07262035B2
公开(公告)日：2007-08-28
申请号：US10062458
申请日：2002-02-05
申请人： Jun Nakamura , Hiroshi Izui , Kayo Moriguchi , Hiroki Kawashima , Tsuyoshi Nakamatsu , Osamu Kurahashi
发明人： Jun Nakamura , Hiroshi Izui , Kayo Moriguchi , Hiroki Kawashima , Tsuyoshi Nakamatsu , Osamu Kurahashi
IPC分类号： C12P13/14 , C12N9/00 , C12N1/20 , C12N15/00 , C07H21/04
CPC分类号： C12N9/0016 , C12N9/1241 , C12N9/93 , C12P13/04 , C12P13/14
摘要： L-Glutamine is produced by culturing a coryneform bacterium which has L-glutamine producing ability and has been modified so that its intracellular glutamine synthetase activity should be enhanced, preferably which has been further modified so that its intracellular glutamate dehydrogenase activity should be enhanced, in a medium to produce and accumulate L-glutamine in the medium and collecting the L-glutamine.
摘要翻译： L-谷氨酰胺通过培养具有L-谷氨酰胺生产能力并已被修饰以使其细胞内谷氨酰胺合成酶活性得到增强的棒状细菌产生，优选已被进一步修饰以使其细胞内谷氨酸脱氢酶活性应当增强，在培养基中产生和积累L-谷氨酰胺并收集L-谷氨酰胺的培养基。

8. 发明授权

US07244569B2 Plasmid autonomously replicable in coryneform bacteria 失效
标题翻译：质粒在棒状细菌中自主复制
公开(公告)号：US07244569B2
公开(公告)日：2007-07-17
申请号：US10832299
申请日：2004-04-27
申请人： Yumi Matsuzaki , Eiichiro Kimura , Tsuyoshi Nakamatsu , Osamu Kurahashi , Yoshio Kawahara , Shinichi Sugimoto
发明人： Yumi Matsuzaki , Eiichiro Kimura , Tsuyoshi Nakamatsu , Osamu Kurahashi , Yoshio Kawahara , Shinichi Sugimoto
IPC分类号： C12N15/74 , C12N15/77 , C12N15/10 , C12N15/31 , C07H21/04 , C07K14/195 , C07K14/34
CPC分类号： C12N15/77
摘要： A plasmid isolatable from Corynebacterium thermoaminogenes, which comprises a gene coding for a Rep protein having the amino acid sequence shown in SEQ ID NO: 8 or an amino acid sequence having homology of 90% or more to the amino acid sequence shown in SEQ ID NO: 8, and has a size of about 4.4 kb or about 6 kb, or a derivative thereof.
摘要翻译：可从热棒状杆菌分离的质粒，其包含编码具有SEQ ID NO：8所示氨基酸序列的Rep蛋白的基因或与SEQ ID NO所示的氨基酸序列具有90％以上同源性的氨基酸序列：8，并且具有约4.4kb或约6kb的大小，或其衍生物。

9. 发明授权

US06905819B1 Plasmid autonomously replicable in coryneform bacteria 失效
标题翻译：质粒在棒状细菌中自主复制
公开(公告)号：US06905819B1
公开(公告)日：2005-06-14
申请号：US09636458
申请日：2000-08-11
申请人： Yumi Matsuzaki , Eiichiro Kimura , Tsuyoshi Nakamatsu , Osamu Kurahashi , Yoshio Kawahara , Shinichi Sugimoto
发明人： Yumi Matsuzaki , Eiichiro Kimura , Tsuyoshi Nakamatsu , Osamu Kurahashi , Yoshio Kawahara , Shinichi Sugimoto
IPC分类号： C12N15/00 , C12N1/21 , C12N15/77 , C12R1/15 , C12N15/10 , C12N1/20 , C12Q1/68
CPC分类号： C12N15/77
摘要： A plasmid which is able to be isolated from Corynebacterium thermoaminogenes, and which comprises a gene coding for a Rep protein having the amino acid sequence shown in SEQ ID NO: 4 or an amino acid sequence having homology of 90% or more to the amino acid sequence shown in SEQ ID NO: 4. and has a size of about 4.4 kb or about 6 kb, or a derivative thereof.
摘要翻译：能够从嗜热棒杆菌分离的质粒，其包含编码具有SEQ ID NO：4所示的氨基酸序列的Rep蛋白的基因或与氨基酸同源性为90％以上的氨基酸序列 SEQ ID NO：4所示的序列，其大小为约4.4kb或约6kb，或其衍生物。

10. 发明授权

US6004773A Method for producing L-lysine 失效
标题翻译：生产L-赖氨酸的方法
公开(公告)号：US6004773A
公开(公告)日：1999-12-21
申请号：US985908
申请日：1997-12-05
申请人： Masayuki Araki , Masakazu Sugimoto , Yasuhiko Yoshihara , Tsuyoshi Nakamatsu
发明人： Masayuki Araki , Masakazu Sugimoto , Yasuhiko Yoshihara , Tsuyoshi Nakamatsu
IPC分类号： C12N1/21 , C12N9/02 , C12N9/10 , C12N9/12 , C12N9/88 , C12N15/53 , C12N15/54 , C12N15/60 , C12N15/77 , C12P13/08 , C12P1/00 , C07H21/04 , C12N15/63 , C12P21/02
CPC分类号： C12N9/001 , C12N15/77 , C12N9/1096 , C12N9/1217 , C12N9/88 , C12P13/08
摘要： A coryneform bacterium harboring an aspartokinase in which feedback inhibition by L-lysine and L-threonine is substantially desensitized, and comprising an enhanced DNA sequence coding for a dihydrodipicolinate reductase, an enhanced DNA sequence coding for dihydropicolinate reductase, an enhance DNA sequence coding for dihydropicolinate synthase, an enhanced DNA sequence coding for diaminopimelate decarboxylase and an enhanced DNA sequence coding for aspartate aminotransferase; a method for producing L-lysine comprising the steps of cultivating the coryneform bacterium in an appropriate medium to allow L-lysine to be produced and accumulated in a culture of the bacterium, and collecting L-lysine from the culture; and a recombinant DNA usable for production of the coryneform bacterium.
摘要翻译：含有赖氨酸和L-苏氨酸的反馈抑制基本脱敏的天冬氨酸激酶的棒状细菌，包含编码二氢吡啶二羧酸还原酶的增强型DNA序列，编码二氢吡啶啉酮还原酶的增强型DNA序列，编码二氢吡啶酸盐的增强型DNA序列合成酶，编码二氨基庚二酸脱羧酶的增强的DNA序列和编码天冬氨酸转氨酶的增强的DNA序列; 一种生产L-赖氨酸的方法，包括以下步骤：在合适的培养基中培养棒状细菌，使L-赖氨酸在细菌培养物中产生并积累，并从培养物中收集L-赖氨酸; 以及可用于生产棒状细菌的重组DNA。

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