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1. 发明授权

US08510053B2 Method for modifying a property of a protein 有权
标题翻译：改变蛋白质性质的方法
公开(公告)号：US08510053B2
公开(公告)日：2013-08-13
申请号：US10933280
申请日：2004-09-03
申请人： Yosuke Nishio , Eiichiro Kimura , Yoshihiro Usuda , Kazuho Ikeo , Yoji Nakamura , Takashi Gojobori , Yutaka Kawarabayashi , Yumi Hino , Eiichi Hori , Jun Yamazaki
发明人： Yosuke Nishio , Eiichiro Kimura , Yoshihiro Usuda , Kazuho Ikeo , Yoji Nakamura , Takashi Gojobori , Yutaka Kawarabayashi , Yumi Hino , Eiichi Hori , Jun Yamazaki
IPC分类号： G01N33/50
CPC分类号： C12N9/88 , C12N9/00 , C12N9/0016 , C12N9/1205 , C12N15/1034 , C12N15/1089
摘要： The present invention relates to a method for modifying a property of a protein. The present invention also relates to a method for producing a protein which has a modified property, and a method for producing a microorganism which has a modified property. The present invention is useful in the field of microbial industrial production and the like.
摘要翻译：本发明涉及一种改变蛋白质性质的方法。本发明还涉及具有改性特性的蛋白质的制造方法，以及具有改性的微生物的制造方法。本发明在微生物工业生产等领域中是有用的。

2. 发明授权

US08944180B2 Power tool with a torque transmitting mechanism 有权
标题翻译：带扭矩传动机构的电动工具
公开(公告)号：US08944180B2
公开(公告)日：2015-02-03
申请号：US13333367
申请日：2011-12-21
申请人： Hiroki Ikuta , Tomohiro Ukai , Yuta Matsuura , Yosuke Nishio
发明人： Hiroki Ikuta , Tomohiro Ukai , Yuta Matsuura , Yosuke Nishio
IPC分类号： B25B21/00 , B25F5/00
CPC分类号： B25B21/00 , B25F5/001
摘要： The power tool has a power transmitting mechanism. When a tool bit is not pressed against a workpiece, the power transmitting mechanism is held in a power transmission interrupted state, and when the tool bit is pressed against the workpiece, the power transmitting mechanism is held in a power transmission state in which the tool bit moves together with the driven-side member in an axial direction of the tool bit so that the driving-side member receives the torque from the driven-side member and the tool bit is driven. Tapered portions are provided between the driving-side member and the driven-side member and inclined with respect to the axial direction of the tool bit. When the driven-side member moves in the axial direction of the tool bit, frictional force is caused on the tapered portions and the torque of the driving-side member is transmitted to the driven-side member by the frictional force.
摘要翻译：电动工具具有动力传递机构。当工具头没有被压在工件上时，动力传递机构保持在动力传递中断状态，并且当工具头被压靠在工件上时，动力传递机构保持在动力传递状态，其中工具与从动侧构件一起沿着工具头的轴向移动，使得驱动侧构件从被驱动侧构件接受扭矩并且驱动工具头。锥形部分设置在驱动侧构件和从动侧构件之间，并且相对于工具头的轴向倾斜。当从动侧构件沿工具头的轴向移动时，在锥形部分上产生摩擦力，并且驱动侧构件的扭矩通过摩擦力传递到从动侧构件。

3. 发明申请

US20120160530A1 POWER TOOL 有权
标题翻译：电动工具
公开(公告)号：US20120160530A1
公开(公告)日：2012-06-28
申请号：US13333367
申请日：2011-12-21
申请人： Hiroki IKUTA , Tomohiro UKAI , Yuta MATSUURA , Yosuke NISHIO
发明人： Hiroki IKUTA , Tomohiro UKAI , Yuta MATSUURA , Yosuke NISHIO
IPC分类号： B25F5/00
CPC分类号： B25B21/00 , B25F5/001
摘要： The power tool has a power transmitting mechanism. When a tool bit is not pressed against a workpiece, the power transmitting mechanism is held in a power transmission interrupted state, and when the tool bit is pressed against the workpiece, the power transmitting mechanism is held in a power transmission state in which the tool bit moves together with the driven-side member in an axial direction of the tool bit so that the driving-side member receives the torque from the driven-side member and the tool bit is driven. Tapered portions are provided between the driving-side member and the driven-side member and inclined with respect to the axial direction of the tool bit. When the driven-side member moves in the axial direction of the tool bit, frictional force is caused on the tapered portions and the torque of the driving-side member is transmitted to the driven-side member by the frictional force.
摘要翻译：电动工具具有动力传递机构。当工具头没有被压在工件上时，动力传递机构保持在动力传递中断状态，并且当工具头压在工件上时，动力传递机构保持在动力传递状态，其中工具与从动侧构件一起沿着工具头的轴向移动，使得驱动侧构件从被驱动侧构件接受扭矩并且驱动工具头。锥形部分设置在驱动侧构件和从动侧构件之间，并且相对于工具头的轴向倾斜。当从动侧构件沿工具头的轴向移动时，在锥形部分上产生摩擦力，并且驱动侧构件的扭矩通过摩擦力传递到从动侧构件。

4. 发明申请

US20050233308A1 Method for modifying a property of a protein 有权
标题翻译：改变蛋白质性质的方法
公开(公告)号：US20050233308A1
公开(公告)日：2005-10-20
申请号：US10933280
申请日：2004-09-03
申请人： Yosuke Nishio , Eiichiro Kimura , Yoshihiro Usuda , Kazuho Ikeo , Yoji Nakamura , Takashi Gojobori , Yutaka Kawarabayashi , Yumi Hino , Eiichi Hori , Jun Yamazaki
发明人： Yosuke Nishio , Eiichiro Kimura , Yoshihiro Usuda , Kazuho Ikeo , Yoji Nakamura , Takashi Gojobori , Yutaka Kawarabayashi , Yumi Hino , Eiichi Hori , Jun Yamazaki
IPC分类号： C12N15/09 , C12N1/21 , C12N9/00 , C12N15/10 , C12P21/02 , C12Q1/00 , G01N33/48 , G01N33/50 , G06F19/00
CPC分类号： C12N9/88 , C12N9/00 , C12N9/0016 , C12N9/1205 , C12N15/1034 , C12N15/1089
摘要： A property of a protein is modified by the following steps: (a) selecting 1000 or more genes from the genome of a first microorganism, and selecting 1000 or more genes from the genome of a second microorganism, wherein the genes from the first microorganism are orthologs to the genes from the second microorganism, and wherein the second microorganism is closely related to the first microorganism, but grows differently under at least one optimum growth condition when compared with the first microorganism, (b) comparing an amino acid sequence encoded by a gene from the first microorganism to an amino acid sequence encoded by the orthologous gene from the second microorganism, (c) detecting substitutions between the amino acid sequence encoded by a gene from the first microorganism and the amino acid sequence encoded by a gene from the second microorganism for each pair of orthologous genes, (d) compiling the detected amino acid substitutions for each amino acid substitution type, (e) calculating the frequency of each amino acid substitution type, wherein for each detected amino acid substitution type, a correction is made by subtracting the total number of substitution types which occur from the first microorganism to the second microorganism from the total number of the same substitution type which occurs in the reverse direction, or from the second microorganism to the first microorganism, (f) identifying and labelling the amino acid substitutions which occur at a high frequency as amino acid substitutions which are involved in said optimum growth condition, and (g) introducing one or more of the amino acid substitutions identified in (f) into the gene encoding the protein to modify a property of the protein.
摘要翻译：通过以下步骤修饰蛋白质的性质：（a）从第一微生物的基因组中选择1000个或更多个基因，并从第二微生物的基因组中选择1000个或更多个基因，其中来自第一个微生物的基因是与第二微生物的基因直系同源，其中第二微生物与第一微生物密切相关，但与第一微生物相比，在至少一个最佳生长条件下生长不同，（b）比较由第一微生物编码的氨基酸序列基因从第一微生物到由第二微生物的直系同源基因编码的氨基酸序列，（c）检测由第一微生物的基因编码的氨基酸序列与由第二微生物的基因编码的氨基酸序列之间的取代每对直系同源基因的微生物，（d）编码每个氨基酸取代类型的检测到的氨基酸取代，（e）cal 规定每个氨基酸取代型的频率，其中对于每个检测到的氨基酸取代类型，通过从相同的取代类型的总数中减去从第一微生物发生到第二微生物的替代类型的总数来进行校正（f）鉴定和标记高频发生的氨基酸取代作为参与所述最佳生长条件的氨基酸取代，和（g）将（f）中鉴定的一个或多个氨基酸取代引入编码蛋白质的基因中以改变蛋白质的性质。

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