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1. 发明授权

US08510053B2 Method for modifying a property of a protein 有权
标题翻译：改变蛋白质性质的方法
公开(公告)号：US08510053B2
公开(公告)日：2013-08-13
申请号：US10933280
申请日：2004-09-03
申请人： Yosuke Nishio , Eiichiro Kimura , Yoshihiro Usuda , Kazuho Ikeo , Yoji Nakamura , Takashi Gojobori , Yutaka Kawarabayashi , Yumi Hino , Eiichi Hori , Jun Yamazaki
发明人： Yosuke Nishio , Eiichiro Kimura , Yoshihiro Usuda , Kazuho Ikeo , Yoji Nakamura , Takashi Gojobori , Yutaka Kawarabayashi , Yumi Hino , Eiichi Hori , Jun Yamazaki
IPC分类号： G01N33/50
CPC分类号： C12N9/88 , C12N9/00 , C12N9/0016 , C12N9/1205 , C12N15/1034 , C12N15/1089
摘要： The present invention relates to a method for modifying a property of a protein. The present invention also relates to a method for producing a protein which has a modified property, and a method for producing a microorganism which has a modified property. The present invention is useful in the field of microbial industrial production and the like.
摘要翻译：本发明涉及一种改变蛋白质性质的方法。本发明还涉及具有改性特性的蛋白质的制造方法，以及具有改性的微生物的制造方法。本发明在微生物工业生产等领域中是有用的。

2. 发明申请

US20050233308A1 Method for modifying a property of a protein 有权
标题翻译：改变蛋白质性质的方法
公开(公告)号：US20050233308A1
公开(公告)日：2005-10-20
申请号：US10933280
申请日：2004-09-03
申请人： Yosuke Nishio , Eiichiro Kimura , Yoshihiro Usuda , Kazuho Ikeo , Yoji Nakamura , Takashi Gojobori , Yutaka Kawarabayashi , Yumi Hino , Eiichi Hori , Jun Yamazaki
发明人： Yosuke Nishio , Eiichiro Kimura , Yoshihiro Usuda , Kazuho Ikeo , Yoji Nakamura , Takashi Gojobori , Yutaka Kawarabayashi , Yumi Hino , Eiichi Hori , Jun Yamazaki
IPC分类号： C12N15/09 , C12N1/21 , C12N9/00 , C12N15/10 , C12P21/02 , C12Q1/00 , G01N33/48 , G01N33/50 , G06F19/00
CPC分类号： C12N9/88 , C12N9/00 , C12N9/0016 , C12N9/1205 , C12N15/1034 , C12N15/1089
摘要： A property of a protein is modified by the following steps: (a) selecting 1000 or more genes from the genome of a first microorganism, and selecting 1000 or more genes from the genome of a second microorganism, wherein the genes from the first microorganism are orthologs to the genes from the second microorganism, and wherein the second microorganism is closely related to the first microorganism, but grows differently under at least one optimum growth condition when compared with the first microorganism, (b) comparing an amino acid sequence encoded by a gene from the first microorganism to an amino acid sequence encoded by the orthologous gene from the second microorganism, (c) detecting substitutions between the amino acid sequence encoded by a gene from the first microorganism and the amino acid sequence encoded by a gene from the second microorganism for each pair of orthologous genes, (d) compiling the detected amino acid substitutions for each amino acid substitution type, (e) calculating the frequency of each amino acid substitution type, wherein for each detected amino acid substitution type, a correction is made by subtracting the total number of substitution types which occur from the first microorganism to the second microorganism from the total number of the same substitution type which occurs in the reverse direction, or from the second microorganism to the first microorganism, (f) identifying and labelling the amino acid substitutions which occur at a high frequency as amino acid substitutions which are involved in said optimum growth condition, and (g) introducing one or more of the amino acid substitutions identified in (f) into the gene encoding the protein to modify a property of the protein.
摘要翻译：通过以下步骤修饰蛋白质的性质：（a）从第一微生物的基因组中选择1000个或更多个基因，并从第二微生物的基因组中选择1000个或更多个基因，其中来自第一个微生物的基因是与第二微生物的基因直系同源，其中第二微生物与第一微生物密切相关，但与第一微生物相比，在至少一个最佳生长条件下生长不同，（b）比较由第一微生物编码的氨基酸序列基因从第一微生物到由第二微生物的直系同源基因编码的氨基酸序列，（c）检测由第一微生物的基因编码的氨基酸序列与由第二微生物的基因编码的氨基酸序列之间的取代每对直系同源基因的微生物，（d）编码每个氨基酸取代类型的检测到的氨基酸取代，（e）cal 规定每个氨基酸取代型的频率，其中对于每个检测到的氨基酸取代类型，通过从相同的取代类型的总数中减去从第一微生物发生到第二微生物的替代类型的总数来进行校正（f）鉴定和标记高频发生的氨基酸取代作为参与所述最佳生长条件的氨基酸取代，和（g）将（f）中鉴定的一个或多个氨基酸取代引入编码蛋白质的基因中以改变蛋白质的性质。

3. 发明授权

US5977331A .alpha.-Ketoglutarate dehydrogenase gene 失效
标题翻译： α-酮戊二酸脱氢酶基因
公开(公告)号：US5977331A
公开(公告)日：1999-11-02
申请号：US750152
申请日：1996-12-12
申请人： Yoko Asakura , Yoshihiro Usuda , Nobuharu Tsujimoto , Eiichiro Kimura , Chizu Abe , Yoshio Kawahara , Tsuyoshi Nakamatsu , Osamu Kurahashi
发明人： Yoko Asakura , Yoshihiro Usuda , Nobuharu Tsujimoto , Eiichiro Kimura , Chizu Abe , Yoshio Kawahara , Tsuyoshi Nakamatsu , Osamu Kurahashi
IPC分类号： C12N1/21 , C12N9/02 , C12N15/53 , C12P13/08 , C12P13/14 , C07H21/02 , C12N1/20 , C12P13/04
CPC分类号： C12N9/0008 , C12P13/08 , C12P13/14
摘要： Disclosed are coryneform L-glutamic acid-producing bacteria deficient in .alpha.-ketoglutarate dehydrogenase, a method of producing L-glutamic acid by using the bacteria, a gene coding for an enzyme having .alpha.-KGDH activity originating from coryneform L-glutamic acid-producing bacteria, recombinant DNA containing the gene, coryneform bacteria harboring the recombinant DNA, and a method of producing L-lysine by using bacteria harboring the recombinant DNA and having L-lysine productivity.
摘要翻译： PCT No.PCT / JP95 / 01131 Sec。 371日期：1996年12月12日 102（e）日期1996年12月12日PCT提交1995年6月7日PCT公布。 WO95 / 34672 PCT公开号日期1995年12月21日公开是α-酮戊二酸脱氢酶缺乏的棒状L-谷氨酸生产菌，使用细菌生产L-谷氨酸的方法，编码具有源自棒状杆菌L的α-KGDH活性的酶的基因谷氨酸生产菌，含有该基因的重组DNA，携带重组DNA的棒状细菌，以及通过使用含有重组DNA并具有L-赖氨酸生产力的细菌产生L-赖氨酸的方法。

4. 发明申请

US20110201062A1 BACTERIUM CAPABLE OF PRODUCING L-AMINO ACID AND METHOD FOR PRODUCING L-AMINO ACID 有权
标题翻译：能生产L-氨基酸的细菌和生产L-氨基酸的方法
公开(公告)号：US20110201062A1
公开(公告)日：2011-08-18
申请号：US13037772
申请日：2011-03-01
申请人： Yousuke Nishio , Yoshihiro Usuda
发明人： Yousuke Nishio , Yoshihiro Usuda
IPC分类号： C12N1/21 , C12P13/14 , C12P13/08 , C12P13/10
CPC分类号： C12P13/08 , C12P13/10 , C12P13/14
摘要： A bacterium is described which belongs to the Enterobacteriaceae family, and has an ability to produce an L-amino acid, such as L-glutamic acid, L-arginine and L-threonine. The bacterium is modified so that the activity of a protein encoded by ydcl gene is decreased, thereby producing and accumulating the L-amino acid selected from L-glutamic acid, L-arginine, and L-threonine in the culture medium or cells of the bacterium when cultured in a culture medium. Subsequently, the L-amino acid is collected from the culture medium or the bacterium.
摘要翻译：描述属于肠杆菌科的细菌，并且具有产生L-氨基酸的能力，例如L-谷氨酸，L-精氨酸和L-苏氨酸。修饰细菌使得由ydcl基因编码的蛋白质的活性降低，从而在培养基或细胞中产生并积累选自L-谷氨酸，L-精氨酸和L-苏氨酸的L-氨基酸细菌培养在培养基中。随后，从培养基或细菌收集L-氨基酸。

5. 发明申请

US20110014663A1 METHOD FOR PRODUCING AN L-AMINO ACID 有权
标题翻译：生产L-氨基酸的方法
公开(公告)号：US20110014663A1
公开(公告)日：2011-01-20
申请号：US12842355
申请日：2010-07-23
申请人： Shigeo Suzuki , Yoshihiro Usuda , Shuhei Hashiro
发明人： Shigeo Suzuki , Yoshihiro Usuda , Shuhei Hashiro
IPC分类号： C12P13/14 , C12P13/04 , C12P13/08
CPC分类号： C12P13/04 , C12P13/08 , C12P13/14
摘要： An L-amino acid is produced by culturing a bacterium having an L-amino acid-producing ability in a medium containing a processed product of a microalga which promotes production and accumulation of the L-amino acid by the bacterium. The process product is produced by disrupting the culture of the microalga, and/or extracting the culture of the microalga, or fractionating the culture of the microalga or the disrupted culture. The processed product contains a mixture of organic substances produced by the microalga, a hydrolysate of the disrupted microalga culture, and/or an extract or fractionation product of the microalga culture. The processed product can also contain a saccarification product of starch or a hydrolysate of fats and oils. The bacterium is cultured to produce and accumulate the L-amino acid in culture, and the L-amino acid is collected from the culture.
摘要翻译：通过在含有促进L-氨基酸的生成和积累的微藻的加工物的培养基中培养具有L-氨基酸生产能力的细菌来生产L-氨基酸。通过破坏微藻的培养和/或提取微藻的培养物，或分馏微藻的培养物或破坏的培养物来生产该过程产物。经加工的产品含有由微藻生产的有机物质，破碎的微藻培养物的水解产物和/或微藻培养物的提取物或分馏产物的混合物。经加工的产品还可以含有淀粉或脂肪和油的水解产物的糖化产物。培养细菌以在培养物中产生和积累L-氨基酸，并从培养物中收集L-氨基酸。

6. 发明申请

US20100112647A1 METHOD FOR PRODUCING AN ACIDIC SUBSTANCE HAVING A CARBOXYL GROUP 审中-公开
标题翻译：生产具有羧基的酸性物质的方法
公开(公告)号：US20100112647A1
公开(公告)日：2010-05-06
申请号：US12579577
申请日：2009-10-15
申请人： Yoshihiko Hara , Keita Fukui , Yoshinori Tajima , Kazue Kawamura , Yoshihiro Usuda , Kazuhiko Matsui
发明人： Yoshihiko Hara , Keita Fukui , Yoshinori Tajima , Kazue Kawamura , Yoshihiro Usuda , Kazuhiko Matsui
IPC分类号： C12P13/20 , C12N1/20 , C12N1/21 , C12P13/14 , C12P7/40 , C12P7/58 , C12P7/42 , C12P7/46 , C12P7/48 , C12P7/50
CPC分类号： C12P13/14 , C07K14/195 , C07K14/245 , C07K14/265 , C12P7/46 , C12P7/48 , C12P7/50 , C12P13/20
摘要： An acidic substance having a carboxyl group is produced by culturing in a medium a microorganism which has been modified to enhance expression of the ybjL gene, and collecting the acidic substance having a carboxyl group from the medium.
摘要翻译：具有羧基的酸性物质是通过在培养基中培养已被改良以增强ybjL基因表达的微生物，并从培养基中收集具有羧基的酸性物质产生的。

7. 发明授权

US07468262B2 Polynucleotides encoding useful polypeptides in corynebacterium glutamicum ssp. lactofermentum 有权
标题翻译：编码谷氨酸棒杆菌中有用多肽的多核苷酸乳发酵
公开(公告)号：US07468262B2
公开(公告)日：2008-12-23
申请号：US10439247
申请日：2003-05-16
申请人： Yoshihiro Usuda , Yousuke Nishio , Kazuhiko Matsui , Shinichi Sugimoto , Chie Koseki
发明人： Yoshihiro Usuda , Yousuke Nishio , Kazuhiko Matsui , Shinichi Sugimoto , Chie Koseki
IPC分类号： C12P13/04 , C12N1/21 , C12N15/63
CPC分类号： C12N9/0016 , C07H21/04 , C07K14/34 , C12N9/1007 , C12N9/22 , C12P13/04 , C12P21/00 , C12P21/02
摘要： The present invention provides novel polypeptides and polynucleotides useful as biotechnological tools, specifically identified in a coryneform bacterium Corynebacterium glutamicum ssp. lactofermentum and methods of producing substances in organisms having enhanced or attenuated expression of these polypeptides and/or polynucleotides.
摘要翻译：本发明提供了用作生物技术工具的新型多肽和多核苷酸，其特异性在棒状杆菌型谷氨酸棒杆菌（Corynebacterium glutamicum）乳发酵和在这些多肽和/或多核苷酸具有增强或减弱的表达的生物体中产生物质的方法。

8. 发明授权

US07220570B2 Polynucleotides encoding polypeptides involved in amino acid biosynthesis in Methylophilus methylotrophus 失效
标题翻译：编码涉及甲基嗜球菌中氨基酸生物合成的多肽的多核苷酸
公开(公告)号：US07220570B2
公开(公告)日：2007-05-22
申请号：US11232405
申请日：2005-09-22
申请人： Yoshihiro Usuda , Yousuke Nishio , Hisashi Yasueda , Shinichi Sugimoto
发明人： Yoshihiro Usuda , Yousuke Nishio , Hisashi Yasueda , Shinichi Sugimoto
IPC分类号： C07H21/04 , C12N9/12 , C12N9/00 , C12P13/04 , C07H21/02 , C12N1/20 , C12N15/00
CPC分类号： C12N15/52 , C07K14/195 , C12N9/1205 , C12N9/93 , C12P13/04
摘要： The present invention provides polypeptides and polynucleotides involved in amino acid biosynthesis in Methylophilus methylotrophus and methods of producing amino acids in microorganisms having enhanced or attenuated expression of these polypeptides and/or polynucleotides.
摘要翻译：本发明提供参与甲基嗜球细胞嗜甲基病毒的氨基酸生物合成的多肽和多核苷酸，以及在这些多肽和/或多核苷酸具有增强或减弱的表达的微生物中产生氨基酸的方法。

9. 发明申请

US20060030010A1 Polynucleotides encoding polypeptides involved in intermediates metabolism of central metabolic pathway in Methylophilus methylotrophus 审中-公开
标题翻译：编码多肽涉及中间代谢途径在甲基嗜球菌中的多核苷酸
公开(公告)号：US20060030010A1
公开(公告)日：2006-02-09
申请号：US11232382
申请日：2005-09-22
申请人： Yoshihiro Usuda , Yousuke Nishio , Hisashi Yasueda , Shinichi Sugimoto
发明人： Yoshihiro Usuda , Yousuke Nishio , Hisashi Yasueda , Shinichi Sugimoto
IPC分类号： C12P13/04 , C07H21/04 , C12N9/10 , C12N15/74 , C12N1/21
CPC分类号： C12N9/00 , C07K14/195 , C12P13/04
摘要： The present invention provides polypeptides and polynucleotides involved in central intermediates metabolism in Methylophilus methylotrophus and methods of producing amino acids in microorganisms having enhanced or attenuated expression of these polypeptides and/or polynucleotides.
摘要翻译：本发明提供参与甲基嗜球细胞嗜甲基病毒的中枢中间体代谢的多肽和多核苷酸以及在具有这些多肽和/或多核苷酸的增强或减弱的表达的微生物中产生氨基酸的方法。

10. 发明授权

US06673576B1 Process for producing nucleic acids 失效
标题翻译：生产核酸的方法
公开(公告)号：US06673576B1
公开(公告)日：2004-01-06
申请号：US08913816
申请日：1998-05-13
申请人： Yoshihiro Usuda , Hisashi Kawasaki , Megumi Shimaoka , Takashi Utagawa
发明人： Yoshihiro Usuda , Hisashi Kawasaki , Megumi Shimaoka , Takashi Utagawa
IPC分类号： C12D1930
CPC分类号： C12N9/12 , C12P19/32
摘要： The present invention relates to a process for producing 5′-inosinic acid or 5′-guanylic acid for use in seasonings or the like from inosine or guanosine or a precursor thereof using an adenosine triphosphate (ATP)-regenerating microorganism containing a DNA encoding a protein that has the activity of forming 5′-inosinic acid or 5′-guanylic acid from inosine or guanosine. Further, the present invention relates to a novel protein having the inosine-guanosine kinase activity, a gene encoding said protein, a recombinant DNA containing said gene, and a microorganism which is transformed with said recombinant DNA.
摘要翻译：本发明涉及一种使用三磷酸腺苷（ATP）再生微生物从肌苷或鸟苷或其前体制备用于调味料等的5'-肌苷酸或5'-鸟苷酸的方法，该微生物含有编码具有从肌苷或鸟苷形成5'-肌苷酸或5'-鸟苷酸活性的蛋白质。此外，本发明涉及具有肌苷 - 鸟苷激酶活性的新型蛋白质，编码所述蛋白质的基因，含有所述基因的重组DNA和用所述重组DNA转化的微生物。

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