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    • 5. 发明申请
    • System and method for processing specimens and images for optical tomography
    • 用于光学层析成像的标本和图像处理系统和方法
    • US20050085721A1
    • 2005-04-21
    • US10964508
    • 2004-10-13
    • Mark FauverJohn RahnEric SeibelAlan Nelson
    • Mark FauverJohn RahnEric SeibelAlan Nelson
    • A61B5/05G02B21/00G02B21/36
    • G02B21/002G02B21/365
    • A scanning method for scanning samples of biological cells using optical tomography includes preparing, acquiring, reconstructing and viewing three-dimensional images of cell samples. Concentration and enrichment of the cell sample follows. The cell sample is stained. Cells are isolated from the cell sample and purified. A cell/solvent mixture is injected into a gel by centrifugation. A cell/gel mixture is injected into a capillary tube until a cell appears centered in a field of view using a step flow method. An optical imaging system, such as a fixed or variable motion optical tomography system acquires a projection image. The sample is rotated about a tube axis to generate additional projections. Once image acquisition is completed, the acquired shawdowgrams or image projections are corrected for errors. A computer or other equivalent processor is used to compute filtered backprojection information for 3D reconstruction.
    • 使用光学断层摄影扫描生物细胞样本的扫描方法包括准备,获取,重建和观察细胞样品的三维图像。 细胞样品的浓缩和浓缩如下。 细胞样品染色。 细胞从细胞样品中分离并纯化。 通过离心将细胞/溶剂混合物注入凝胶。 将细胞/凝胶混合物注入毛细管中,直到细胞在视野中以步进流动法出现中心。 诸如固定或可变运动光学断层摄影系统的光学成像系统获取投影图像。 样品围绕管轴旋转以产生额外的突起。 一旦完成图像采集,就可以纠正所获取的肖像图或图像投影。 计算机或其他等效处理器用于计算用于3D重建的滤波反投影信息。