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1. 发明申请

WO2022183303A1 A METHOD FOR PREPARATION OF α-GLUCAN 审中-公开
公开(公告)号：WO2022183303A1
公开(公告)日：2022-09-09
申请号：PCT/CA2022/050554
申请日：2022-04-08
申请人： ZHU, Jianhua , LI, Xiaolin
发明人： ZENG, Jinghua
IPC分类号： C12P19/04 , A23L33/21 , C08B37/02 , C12N1/20 , C12N9/46 , C12P19/08
摘要： A production method of low molecular weight ɑ-glucan includes: inoculating an activated Leuconostoc mesenteroides in a 5L fermentor at a 10% inoculum. Fermentation broth is placed in the fermentor at an initial pH of 6.8-7.0, temperature of 25 ℃ to 28℃, stirring speed at 120r/min, and fermented for 20-40 hours. Dextranase is added after 5-30 hours of fermentation at a dosage of 1/10,000 to 5/10,000 by volume. The molecular weight of ɑ-glucan is controlled within 10000D by the amount of enzyme added, and the total fermentation process is about 20-40 hours. After the reaction is terminated, the fermentation liquid is concentrated and dried to prepare dietary fiber products with a molecular weight of 500-5000D. The viscosity of the fermentation liquid and concentration of ɑ-glucan in the fermentation liquid may be reduced to promote the forward reaction, accelerate the sucrose conversion rate and increase the product yield.

2. 发明申请

WO2015183724A1 ENZYMATIC SYNTHESIS OF SOLUBLE GLUCAN FIBER 审中-公开
标题翻译：可溶性葡萄糖纤维的酶合成
公开(公告)号：WO2015183724A1
公开(公告)日：2015-12-03
申请号：PCT/US2015/032133
申请日：2015-05-22
申请人： E. I. DU PONT DE NEMOURS AND COMPANY
发明人： CHENG, Qiong , DICOSIMO, Robert , OUWEHAND, Arthur , YOIU, Zheng , PAYNE, Mark S. , PRASAD, Jahnavi Chandra
IPC分类号： C12P19/18 , C12P19/08 , C08B37/00 , C12P19/04 , A23L1/054 , A61K8/36 , A61K31/716
CPC分类号： C12P19/18 , A23G1/40 , A23G3/42 , A23G9/34 , A23K20/10 , A23L2/52 , A23L2/60 , A23L5/00 , A23L7/00 , A23L7/139 , A23L9/00 , A23L21/00 , A23L27/30 , A23L29/30 , A23L29/37 , A23L33/10 , A23L33/105 , A23L33/115 , A23L33/125 , A23L33/17 , A23L33/18 , A23L33/21 , A23V2002/00 , A61K8/60 , A61K8/73 , A61K31/702 , A61K31/716 , A61K45/06 , A61K2800/10 , A61K2800/85 , A61Q19/00 , C07H3/06 , C08B37/0009 , C08L5/00 , C12P19/04 , C12P19/08 , C12Y204/01005 , C12Y301/01059 , C12Y302/01011 , C12Y302/01059 , A61K2300/00
摘要： An enzymatically produced soluble α-glucan fiber composition is provided suitable for use as a digestion resistant fiber in food and feed applications. The soluble α-glucan fiber composition can be blended with one or more additional food ingredients to produce fiber-containing compositions. Methods for the production and use of compositions comprising the soluble α-glucan fiber are also provided.
摘要翻译：提供了一种酶促生产的可溶性α-葡聚糖纤维组合物，适合用作食品和饲料应用中的消化纤维。可溶性α-葡聚糖纤维组合物可以与一种或多种另外的食物成分混合以制备含纤维组合物。还提供了生产和使用包含可溶性α-葡聚糖纤维的组合物的方法。

3. 发明申请

WO2013017330A1 NEW METHOD FOR MANUFACTURE OF DEXTRAN, DEXTRAN SOLUTION OBTAINED, AND USES 审中-公开
标题翻译： DEXTRAN，DEXTRAN溶液的制造新方法及用途
公开(公告)号：WO2013017330A1
公开(公告)日：2013-02-07
申请号：PCT/EP2012/061619
申请日：2012-06-18
申请人： S.P.C.M. SA , WIESER, Marco
发明人： WIESER, Marco
IPC分类号： C12P19/08 , B01D21/01 , C01F7/34 , C08B37/00 , C08L5/02 , C01F7/06
CPC分类号： C12P19/08 , C01F7/0653 , C01F7/144 , C08B37/0003 , C08B37/0021 , C08L5/02
摘要： Microbiological method of production of a dextran solution, according to which a culture medium containing sucrose is inoculated with a preculture of a bacterial strain that is able to produce dextran, then the dextran solution obtained at the end of fermentation is recovered directly, without a subsequent concentration step, characterized in that: - before inoculation, the culture medium contains at least 10 wt.% of sucrose, - after inoculation, sucrose is added again in conditions such that the total amount of sucrose in the medium, including that present before inoculation, is at least 16 wt.%, - the dextran solution obtained contains at least 10 wt.% of dextran. The native solution of dextran obtained and use of the solution as a flocculant.
摘要翻译：生产葡聚糖溶液的微生物学方法，其中含有能够产生葡聚糖的细菌菌株的预培养将含有蔗糖的培养基接种，然后直接回收在发酵结束时获得的葡聚糖溶液，而不用随后浓缩步骤，其特征在于：在接种之前，培养基含有至少10重量％的蔗糖， - 接种后，再次加入蔗糖，使得培养基中的蔗糖总量，包括接种前存在的蔗糖总量，至少为16重量％， - 获得的葡聚糖溶液含有至少10重量％的葡聚糖。获得的葡聚糖的天然溶液和溶液用作絮凝剂。

4. 发明申请

WO2011140212A1 FAT REPLACERS AND FILLING MATERIALS 审中-公开
标题翻译：脂肪替代物和填充材料
公开(公告)号：WO2011140212A1
公开(公告)日：2011-11-10
申请号：PCT/US2011/035180
申请日：2011-05-04
申请人： CARGILL, INCORPORATED , DENDOOVEN, Els, Ginette, Alexander , NGUYEN, Van, Sau , VAN-NIEUWENHUYZE, Lutgart , VERCAUTEREN, Ronny, Leontina, Marcel
发明人： DENDOOVEN, Els, Ginette, Alexander , NGUYEN, Van, Sau , VAN-NIEUWENHUYZE, Lutgart , VERCAUTEREN, Ronny, Leontina, Marcel
IPC分类号： A23L1/054 , A23L1/30 , A23L1/307 , A23L1/308 , C08B37/00 , C12P19/08 , C12P19/18 , C07H3/06 , C12N9/10 , A21D2/18
CPC分类号： A23L29/273 , A21D2/181 , A23L33/10 , A23L33/20 , A23L33/21 , A23V2002/00 , C08B37/0021 , C12P19/08 , C12P19/18 , C12Y204/01005 , A23V2200/124 , A23V2250/28 , A23V2250/5042
摘要： The present invention relates generally to fat replacers and their use in various food products. Aspects of the disclosure are particularly directed to oligodextran-based fat replacers that are lower in calories, heat stable, and increase fiber. They can either be used alone or in combination with other additives to decrease the fat content while maintaining good organoleptic properties.
摘要翻译：本发明一般涉及脂肪替代物及其在各种食品中的用途。本公开的方面特别涉及低聚糖基脂肪替代物，其更低的热量，热稳定性和增加纤维。它们可以单独使用或与其他添加剂组合使用，以降低脂肪含量，同时保持良好的感官特性。

5. 发明申请

WO2022121962A1 杂聚寡糖及其在提高植物抗病性中的应用审中-公开
公开(公告)号：WO2022121962A1
公开(公告)日：2022-06-16
申请号：PCT/CN2021/136586
申请日：2021-12-08
申请人：南京理工大学 NANJING UNIVERSITY OF SCIENCE AND TECHNOLOGY [CN]/[CN]
发明人：张建法 ZHANG, Jianfa , 付任杰 FU, Renjie , 李晶 LI, Jing , 程瑞 CHENG, Rui
IPC分类号： C08B37/02 , C12P19/08 , C12P1/00 , C12N1/20 , C12P19/04 , A01N43/16 , A01N43/90 , A01P21/00 , C12R1/41
摘要：本发明公开了一种杂聚寡糖及其在提高植物抗病性中的应用。所述的杂聚寡糖含有7个D-葡萄糖残基和1个D-半乳糖残基，每个杂聚寡糖分子含有或不含有一分子的丙酮酰基团，和一分子的琥珀酰基团，其结构为式（I）。本发明的杂聚寡糖能够诱导植物叶片产生H2O2，增强植物抗性酶的活性，激发植物的植保素的产生，从而提高植物对病原菌感染的抗性，适用于防治病原菌引起的多种农作物和植物的病害。

6. 发明申请

WO2017091533A1 POLYPEPTIDES CAPABLE OF PRODUCING GLUCANS HAVING ALPHA-1,2 BRANCHES AND USE OF THE SAME 审中-公开
标题翻译：能够产生具有α-1,2分支的葡聚糖的多肽及其用途
公开(公告)号：WO2017091533A1
公开(公告)日：2017-06-01
申请号：PCT/US2016/063233
申请日：2016-11-22
申请人： E. I. DU PONT DE NEMOURS AND COMPANY
发明人： CHENG, Qiong , DICOSIMO, Robert , PRASAD, Jahnavi Chandra , ZHANG, Zhenghong
IPC分类号： A23L33/10 , A61K31/716 , C08B37/00 , C12P19/04 , C12P19/08 , C12P19/16 , C12P19/18 , C12N9/10 , C08L5/00 , C08L5/02 , A23L33/135 , A23L33/21 , A61K8/73
CPC分类号： A23L33/10 , A23L33/135 , A23L33/21 , A23V2002/00 , A23V2200/00 , A61K8/60 , A61K8/64 , A61K8/73 , A61K31/716 , A61K2800/10 , A61Q19/00 , C08B37/0009 , C08L5/00 , C08L5/02 , C12N9/1051 , C12P19/04 , C12P19/08 , C12P19/16 , C12P19/18 , C12Y204/00 , C12Y204/01 , C12Y204/01005 , C12Y302/01 , A23V2200/32 , A23V2200/3202 , A23V2200/328 , A23V2250/00 , A23V2250/282 , A23V2250/284 , A23V2250/5116
摘要： Disclosed herein are proteins capable of forming glucans having alpha-1,2 linkages/branches, reactions and methods for producing such glucan, compositions comprising such glucan, and various applications thereof.
摘要翻译：本文公开了能够形成具有α-1,2键/分支的葡聚糖的蛋白质，用于生产这种葡聚糖的反应和方法，包含这种葡聚糖的组合物，以及其各种应用。

7. 发明申请

WO2015130881A1 ENZYMATIC HYDROLYSIS OF DISACCHARIDES AND OLIGOSACCHARIDES USING ALPHA-GLUCOSIDASE ENZYMES 审中-公开
标题翻译：使用阿尔法 - 葡萄糖苷酶的酶解和低聚糖的酶促水解
公开(公告)号：WO2015130881A1
公开(公告)日：2015-09-03
申请号：PCT/US2015/017644
申请日：2015-02-26
申请人： E. I. DU PONT DE NEMOURS AND COMPANY
发明人： NAGY, Kevin D. , HAGO, Erwin Columbus , SHETTY, Jayarama K. , HENNESSEY, Susan Marie , DICOSIMO, Robert , HUA, Ling , RAMIREZ, Rodrigo , TANG, Zhongmei , YU, Zheyong
IPC分类号： C12P19/16 , C12P19/18 , C12P19/04 , C12P19/12 , C12P19/08
CPC分类号： C12P19/16 , C07H3/06 , C12N9/1051 , C12N9/2402 , C12N9/2428 , C12P19/02 , C12P19/04 , C12P19/08 , C12P19/12 , C12P19/18 , C12Y204/01005 , C12Y204/01024 , C12Y302/01003 , C12Y302/0102 , C12Y302/0107 , C12Y302/01084 , C12Y302/01115
摘要： A method is disclosed for hydrolyzing an alpha-1,3 or alpha-1,6 glucosyl-glucose linkage in a saccharide (disaccharide or oligosaccharide). This method comprises contacting the saccharide with an alpha-glucosidase enzyme such as transglucosidase under suitable conditions, during which contacting step the enzyme hydrolyzes at least one alpha-1,3 or alpha-1,6 glucosyl-glucose linkage of the saccharide. This method is useful for reducing the amount of oligosaccharides in a filtrate isolated from a glucan synthesis reaction, for example.
摘要翻译：公开了用于水解糖类（二糖或寡糖）中的α-1,3或α-1,6糖基 - 葡萄糖键的方法。该方法包括在合适的条件下使糖与α-葡糖苷酶如转葡糖苷酶接触，在该步骤期间酶水解糖的至少一个α-1,3或α-1,6葡糖基 - 葡萄糖键。该方法例如可用于减少从葡聚糖合成反应分离的滤液中的寡糖的量。

8. 发明申请

WO2006096269A3 TARGETED GENE DELETIONS FOR POLYSACCHARIDE SLIME FORMERS 审中-公开
标题翻译：针对多糖制剂的目标基因删除
公开(公告)号：WO2006096269A3
公开(公告)日：2008-12-04
申请号：PCT/US2006003982
申请日：2006-02-03
申请人： CP KELCO US INC
发明人： HARDING NANCY E , YAMINI PATEL , COLEMAN RUSSELL J
IPC分类号： C12Q1/68 , A01N43/04 , A23G4/18 , A61K9/14 , C07G99/00 , C12N1/20 , C12N9/10 , C12N15/00 , C12N15/74 , C12P19/04 , C12P19/08 , C12P19/34
CPC分类号： C12N15/74 , C07K14/195 , C12N15/52 , C12P19/04
摘要： Sphingomonas strains have extracellular polysaccharide (e.g., gellan, diutan) that is firmly attached to the cell surface. This attachment may limit polysaccharide production by impairing uptake of nutrients into the cell or due to limited sites for polysaccharide biosynthesis on the cell surface. Two genes for polysaccharide biosynthesis, designated gelM and gelN in gellan- producing strains and dpsM and dpsN in diutan-producing strains, have been inactivated by deletion mutations and shown to produce polysaccharide that is not firmly attached to the cell surface, i.e., slime form. Another gene for polysaccharide biosynthesis, designated gell in gellan producing strains, was inactivated by insertion mutation and also shown to produce the slime phenotype. The homologous gene dpsl in the diutan producing strain should also be involved in the attachment of the polysaccharide to the cell surface. The slime characteristic was demonstrated by the ability of the cells to be centrifuged and the lack of cell clumping as seen under the microscope or in diluted suspensions. The diutan slime mutants had somewhat increased productivity and the recovered diutan product had significantly improved rheology. Gellan slime mutants had lower broth viscosity which facilitates mixing during fermentation; however, the recovered gellan product had lower gel strength than the gellan produced from a capsular strain. A deletion in a gene gelR, which encodes a protein with homology to surface proteins and outer membrane proteins and weak homology to proteins with polysaccharide.
摘要翻译：鞘氨醇单胞菌属菌株具有牢固附着于细胞表面的细胞外多糖（例如，结冷糖，二氘）。该附件可能通过损害营养物质进入细胞或由于细胞表面上多糖生物合成的有限位点而限制多糖产生。用于多糖生物合成的两个基因，在凝胶产生菌株中命名为gelM和gelN，在diutan产生菌株中命名为dpsM和dpsN，已被缺失突变失活，并显示产生不牢固附着于细胞表面的多糖，即粘液形式。用于多糖生物合成的另一个基因，在凝胶产生菌株中称为凝胶，通过插入突变失活，并且还显示产生粘液表型。二萜生产菌株中的同源基因dps1也应参与多糖与细胞表面的连接。通过细胞离心的能力和在显微镜下或在稀释的悬浮液中观察到的细胞团聚的缺乏证明了粘液特性。 di an sl ime mut mut ants。。。。。。。。。。。。。。。。。。。。凝胶粘液突变体具有较低的肉汤粘度，有利于发酵期间的混合; 然而，回收的结冷胶产品的凝胶强度低于从荚膜菌株产生的结冷胶。在基因gelR中的缺失，其编码与表面蛋白质和外膜蛋白质具有同源性的蛋白质以及与多糖蛋白质的弱同源性。

9. 发明申请

WO0142309A8 METHOD OF INCREASING THE alpha -AMYLASE-RESISTANT STARCH CONTENT (RS CONTENT) OF A POLYSACCHARIDE, POLYSACCHARIDES, THE USE THEREOF AND FOOD CONTAINING SAID POLYSACCHARIDES 审中-公开
标题翻译：法提高α-淀粉酶耐压强度（RS-SALARY）多糖，多糖，它们的用途和食物的含量随这些多糖
公开(公告)号：WO0142309A8
公开(公告)日：2001-07-12
申请号：PCT/EP0012459
申请日：2000-12-09
申请人： CELANESE VENTURES GMBH , BENGS HOLGER , JACOBASCH GISELA , SCHMIEDL DETLEF
发明人： BENGS HOLGER , JACOBASCH GISELA , SCHMIEDL DETLEF
IPC分类号： A23L1/308 , C08B30/12 , C08B37/00 , C12P19/08 , C08B
CPC分类号： C08B30/12
摘要： The invention relates to a method of increasing the alpha -amylase-resistant starch content (RS content) of a polysaccharide. The inventive method is characterized by incubating the polysaccharide used with less than an equivalent weight of water, cooling it, optionally drying it and obtaining a polysaccharide that has an increased RS content vis-à-vis the polysaccharide used.
摘要翻译：本发明涉及一种用于将多糖，其特征在于，温育在水缺乏使用的，然后冷却多糖，任选地进行干燥和多糖相对于所述的增加的α-淀粉酶抗性淀粉（RS含量）的含量多糖就业人口增加RS含量胜。

10. 发明申请

WO9504155A3 A METHOD FOR SYNTHESIS OF POLYSACCHARIDES 审中-公开
标题翻译：一种合成多糖的方法
公开(公告)号：WO9504155A3
公开(公告)日：1995-03-16
申请号：PCT/US9408731
申请日：1994-08-02
申请人： BROWN MALCOLM R JR , KOBAYASHI SHIRO , KUDLICKA KRYSTYNA , KUGA SHIGENORI , LEE JONG , LI LIKUN , OKUDA KAZUO , SHODA SHIN ICHIRO
发明人： BROWN R MALCOLM JR , KOBAYASHI SHIRO , KUDLICKA KRYSTYNA , KUGA SHIGENORI , LEE JONG , LI LIKUN , OKUDA KAZUO , SHODA SHIN-ICHIRO
IPC分类号： C12N9/10 , C12N9/24 , C12N11/12 , C12P19/04 , C12P19/08 , C12P19/18 , C12N11/06
CPC分类号： C12P19/08 , C12N9/10 , C12N9/2437 , C12N11/12 , C12P19/04 , C12Y302/01004
摘要： Methods of synthesizing cellulose I in vitro are disclosed. One method comprises contacting an activated saccharide substrate with an endoglucanase in an appropriate organic solvent/buffer ratio. The invention also encompasses a purified endoglucanase. A second method comprises contacting a nucleotide sugar with a purified glycosyl transferase in an appropriate buffer medium to insure polymerization and crystallization of parallel chains from the enzyme/micelle complex to form cellulose I.

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