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1. 发明申请

WO2022213721A1 人工油体固定化多酶生产塔格糖的方法审中-公开
公开(公告)号：WO2022213721A1
公开(公告)日：2022-10-13
申请号：PCT/CN2022/076063
申请日：2022-02-11
申请人：中国科学院天津工业生物技术研究所
发明人：马延和 , 石婷 , 韩平平 , 李运杰
IPC分类号： C12N11/18 , C12N11/06 , C12P19/24 , C12P19/18 , C12P19/16 , C12P19/02
摘要：提供了一种基于人工油体固定化多酶及其在塔格糖制备中的应用，具体为使用人工油体将表达的目标蛋白酶-油体蛋白的融合蛋白与油体混合，超声处理，通过人体蛋白的特异疏水性将融合蛋白锚定到油体表面，形成含有目标蛋白酶的人工油体，从而实现酶的纯化和固定化同步完成。所述可用于塔格糖生产的固定化多酶采用人工油体作为固定化酶基质，显著提高固定化酶的稳定性，降低了当前酶法制备塔格糖的生产成本，且制备工艺简单。

2. 发明申请

WO2021232667A1 一种淀粉基结肠靶向控释晶体包合物及其制备方法审中-公开
公开(公告)号：WO2021232667A1
公开(公告)日：2021-11-25
申请号：PCT/CN2020/121746
申请日：2020-10-19
申请人：华南理工大学 , 广州现代产业技术研究院
发明人：罗志刚 , 王萍萍
IPC分类号： A61K47/69 , A61K31/05 , A61K31/593 , A61K31/015 , A61P1/00 , A61P39/06 , A61P29/00 , C12P19/16 , C12P19/04 , C08B30/18 , A23L29/30 , A23L33/105 , A23L33/155
摘要：一种淀粉基结肠靶向控释晶体包合物及其制备方法。首次通过共结晶技术对螺旋糊精/脂溶性营养素包合物进行共结晶沉淀，包合物能够抵抗胃与小肠的消化吸收，实现脂溶性营养素在结肠靶向释放。以天然多糖高分子为原料，价廉易得，生物相容性好，拓宽了淀粉的应用领域，在结肠靶向控释载体方面具有重要的应用价值。

3. 发明申请

WO2018118854A1 CONTINUOUS PROCESS FOR HYDROGENATION OF MALTOSE TO MALTITOL 审中-公开
公开(公告)号：WO2018118854A1
公开(公告)日：2018-06-28
申请号：PCT/US2017/067208
申请日：2017-12-19
申请人： ARCHER DANIELS MIDLAND COMPANY
发明人： BLOOM, Paul, D. , MA, Chi Cheng , MARTIN, Kevin
IPC分类号： C12P19/14 , C12P19/16 , C12P19/22
摘要： A process for preparing maltitol is described. The process enables greater control of the reaction to minimized generation of glucose and sorbitol side products that can arise from the over-hydrogenation of maltitol. The process involves reacting a medium containing maltose at a concentration of ≤ 30% with hydrogen in a continuous manner using a fixed-bed reactor at a reaction temperature and pressure sufficient to produce a final product containing a yield of maltitol of at least 90 mol.%, at a conversion rate in which no more than about 2 mol.% of said maltose concentration remains, and a sorbitol concentration of less than 1.0 mol.%.

4. 发明申请

WO2015130883A9 ENZYMATIC HYDROLYSIS OF DISACCHARIDES AND OLIGOSACCHARIDES USING ALPHA-GLUCOSIDASE ENZYMES 审中-公开
标题翻译：使用阿尔法 - 葡萄糖苷酶的酶解和低聚糖的酶促水解
公开(公告)号：WO2015130883A9
公开(公告)日：2016-08-18
申请号：PCT/US2015017648
申请日：2015-02-26
申请人： DU PONT
发明人： NAGY KEVIN D , HAGO ERWIN COLUMBUS , SHETTY JAYARAMA K , HENNESSEY SUSAN MARIE , DICOSIMO ROBERT , HUA LING , RAMIREZ RODRIGO , TANG ZHONGMEI , YU ZHEYONG
IPC分类号： C12P19/16 , C12P19/04 , C12P19/08 , C12P19/12 , C12P19/18
CPC分类号： C12P19/16 , C07H3/06 , C12N9/1051 , C12N9/2402 , C12N9/2428 , C12P19/02 , C12P19/04 , C12P19/08 , C12P19/12 , C12P19/18 , C12Y204/01005 , C12Y204/01024 , C12Y302/01003 , C12Y302/0102 , C12Y302/0107 , C12Y302/01084 , C12Y302/01115
摘要： A method is disclosed for hydro!yzing an alpha-1,5 giucosyl-fructose linkage in a saccharide (disaccharide or oligosaccharide) such as leucrose. This method comprises contacting the saccharide with an alpha-glucosidase enzyme such as transgiucosidase or glucoamylase under suitable conditions, during which contacting step the enzyme hydrolyzes at least one aipha-1,5 glucosyl-fructose linkage of the saccharide. This method is useful for reducing the amount of leucrose in a filtrate isolated from a glucan synthesis reaction, for example.
摘要翻译：公开了一种在糖（二糖或寡糖）如白芥糖中加氢α-1,5糖基 - 果糖键的方法。该方法包括在合适的条件下将糖与α-葡糖苷酶如转谷氨酰胺酶或葡糖淀粉酶接触，在此步骤期间酶水解糖的至少一种aipha-1,5葡糖基 - 果糖键。该方法例如可用于减少从葡聚糖合成反应分离的滤液中的白兰地的量。

5. 发明申请

WO2015110473A3 PULLULANASE VARIANTS AND POLYNUCLEOTIDES ENCODING SAME 审中-公开
标题翻译： PULLULANASE变体和编码它的多核苷酸
公开(公告)号：WO2015110473A3
公开(公告)日：2015-09-17
申请号：PCT/EP2015051145
申请日：2015-01-21
申请人： NOVOZYMES AS
发明人： MATSUI TOMOKO , YAMAGISHI AKIHIKO
IPC分类号： C12N9/44 , A23L1/09 , C12P19/16
CPC分类号： C12N9/2457 , C12P7/06 , C12P19/02 , C12P19/16 , C12P2203/00 , C12Y302/01041 , Y02E50/17
摘要： The present invention relates to pullulanase variants having increased thermoactivity and comprising substitutions of the parent pullulanase at one or more positions corresponding to positions 393, 143, 150, 243, 244, 345, 346, 368, 370, 373, 381, 382, 385, 387, 402, 429, 430, 431, 432, 456, 486, 492, 610, 624, 631, 632, 665 and 699 of the polypeptide of SEQ ID NO: 3. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants. The variants are mainly derived from pullulanases from Bacillus deramificans, Bacillus acidopullulyticus or hybrid pullulanases.
摘要翻译：本发明涉及具有增加的热活性的支链淀粉酶变体，并且包括在对应于位置393,143,150,243,244,345,346,368,370,373,381,368,385,337,381,382,385的一个或多个位置上的母体支链淀粉酶的取代，387,402,49,430,431,432,456,468,492,610,624,626,632,665和699的多核苷酸。本发明还涉及编码变体的多核苷酸 ; 核酸构建体，载体和包含多核苷酸的宿主细胞; 以及使用这些变体的方法。变异体主要衍生自来自芽孢杆菌属（Bacillus deramificans），Bacillus acidulullulyticus或杂合支链淀粉酶的支链淀粉酶。

6. 发明申请

WO2013114223A3 PROCESS FOR PRODUCING MALTITOL FROM STARCH 审中-公开
标题翻译：从淀粉生产MALTITOL的方法
公开(公告)号：WO2013114223A3
公开(公告)日：2014-01-23
申请号：PCT/IB2013000962
申请日：2013-01-24
申请人： CARGILL INC
发明人： FURLAN TIZIANO , NATALONI LUIGI , TOLOMELLI PATRIZIA
IPC分类号： C12P19/12 , C07H15/04 , C12P19/14 , C12P19/16 , C12P19/22
CPC分类号： C12P19/22 , C07H15/04 , C12P19/12 , C12P19/14 , C12P19/16
摘要： The present invention relates to a process for producing a syrup which is rich in maltitol. The process is comprising the successive steps of liquefaction of a starch milk and saccharification of the liquefied starch milk in the presence of alpha-amylase, beta-amylase and a debranching enzyme selected from the group of pullulanase, iso-amylase and mixtures thereof, preferably pullulanase and further adding maltogenic alpha-amylase and/or iso-amylase, for obtaining a maltose containing syrup comprising at least 85% maltose based on dry matter and less than 1.5% glucose based on dry matter, preferably less than 1 % glucose based on dry matter, followed by molecular sieving of the maltose containing syrup to obtain a fraction (A) comprising at least 95% maltose based on dry substance of fraction (A), and further hydrogenating catalytically fraction (A) for obtaining a liquid maltitol enriched product (B).
摘要翻译：本发明涉及富含麦芽糖醇的糖浆的制造方法。该方法包括在α-淀粉酶，β-淀粉酶和选自支链淀粉酶，异淀粉酶及其混合物的脱支酶的存在下，液化淀粉乳和液化淀粉的糖化的连续步骤，优选地支链淀粉酶和进一步添加麦芽糖α-淀粉酶和/或异淀粉酶，用于获得含麦芽糖的糖浆，其包含基于干物质的至少85％的麦芽糖和基于干物质的小于1.5％的葡萄糖，优选小于1％的葡萄糖，基于干燥物质，然后进行含有麦芽糖的糖浆的分子筛分，得到基于馏分（A）的干物质的至少95％麦芽糖的馏分（A），并进一步氢化催化馏分（A）以获得液体麦芽糖醇富集产物（B）。

7. 发明申请

WO2012124520A1 改変型α－グルコシダーゼ及びその用途审中-公开
标题翻译：改性的LPHA-葡萄糖苷酶及其应用
公开(公告)号：WO2012124520A1
公开(公告)日：2012-09-20
申请号：PCT/JP2012/055558
申请日：2012-03-05
申请人：天野エンザイム株式会社 , 石原聡
发明人：石原　聡
IPC分类号： C12N15/09 , C12N1/15 , C12N1/19 , C12N1/21 , C12N5/10 , C12N9/24 , C12P19/16 , C12P19/18
CPC分类号： C12N9/2408 , C12N9/1051 , C12N9/2428 , C12P19/04 , C12P19/16 , C12P19/18 , C12P19/20 , C12Y302/0102 , Y02P20/52
摘要：糖転移活性が優位なα－グルコシダーゼ及びその用途などを提供することを課題とする。α－グルコシダーゼのアミノ酸配列において、以下の群より選択される一又は二以上のアミノ酸が他のアミノ酸に置換されたアミノ酸配列からなる改変型α－グルコシダーゼ：配列番号１に示すアミノ酸配列の385位、491 位、535 位、450 位、534 位、537 位、538 位、554 位、556 位、630位、683 位若しくは689 位のアミノ酸に相当するアミノ酸、又は、配列番号２に示すアミノ酸配列の579 位若しくは585 位アミノ酸に相当するアミノ酸。
摘要翻译：本发明解决了提供具有显性转糖基活性的α-葡萄糖苷酶和α-葡糖苷酶的应用的问题。该修饰的α-葡糖苷酶包含氨基酸序列，其中一个或多个选自下列基团的氨基酸已被α-葡糖苷酶的氨基酸序列中的另一个氨基酸取代：对应于氨基酸位置的氨基酸由SEQ ID NO：1表示的氨基酸序列的385,491,535,450,534,537,538,554,556,630,683或689或对应于SEQ ID NO：1的氨基酸位置579或585的氨基酸由SEQ ID NO：2表示的氨基酸序列。

8. 发明申请

WO0063445A8 D-GALACTOSE COMPOSITION AND PROCESS FOR ITS MANUFACTURE 审中-公开
标题翻译： D-胶体组合物及其制备方法
公开(公告)号：WO0063445A8
公开(公告)日：2001-11-22
申请号：PCT/NL0000237
申请日：2000-04-12
申请人： CARGILL BV , MOLLEE PETER WILLEM , KEMPENER SASKIA
发明人： MOLLEE PETER WILLEM , KEMPENER SASKIA
IPC分类号： C12P19/02 , C12P19/16 , C13K13/00
CPC分类号： C13K13/00 , C12P19/02 , C12P19/16
摘要： The invention relates to procedures for the manufacturing of D-galactose and D-galactose containing compositions. More specifically, the invention provides a method for isolating D-galactose from legume material, which method comprises subjecting a legume composition containg non-homologous soluble polysaccharide, commonly referred to as oligosaccharide, to one or more treatments, resulting in a preparation comprising at least 30 % oligosaccharides, and subsequent hydrolysis of the oligosaccharides in this preparation into mainly monosaccharides.
摘要翻译：本发明涉及制备含D-半乳糖和D-半乳糖的组合物的方法。更具体地说，本发明提供了从豆科植物材料中分离D-半乳糖的方法，该方法包括将含有通常称为寡糖的非同源可溶性多糖的豆类组合物进行一次或多次处理，得到至少包含至少 30％的寡糖，随后将该制剂中的寡糖水解成主要是单糖。

9. 发明申请

WO00066633A1 BRANCHED GLUCOSE SOLUBLE POLYMERS AND METHOD FOR THE PRODUCTION THEREOF 审中-公开
标题翻译：分支葡萄糖可溶性聚合物及其生产方法
公开(公告)号：WO00066633A1
公开(公告)日：2000-11-09
申请号：PCT/FR2000/001109
申请日：2000-04-26
IPC分类号： A23L1/09 , C08B30/12 , C08B30/18 , C08B31/00 , C08B37/00 , C12P19/04 , C12P19/16
CPC分类号： C08B37/00 , C08B30/18 , C12P19/16
摘要： The invention relates to glucose soluble polymers which do not substantially contain any beta glucosidic bonds, characterized in that they comprise 2.5 -10 % alpha -1,6 gluocosidic bonds, have a very low or zero tendency to retrograde in an aqueous solution determined according to a test A, possess an MP which is determined according to a test C having a median value of the distribution profile of the molecular masses ranging from 104 and 105 Daltons and have a reducing sugar content that is at most 9 %.
摘要翻译：本发明涉及基本上不含有任何β糖苷键的葡萄糖可溶性聚合物，其特征在于它们包含2.5-10％的α-1,6糖苷键，在根据以下方法确定的水溶液中具有非常低或零的逆向倾向：测试A具有根据具有104和105道尔顿分子量分布曲线的中值的测试C确定的MP，并且具有至多9％的还原糖含量。

10. 发明申请

WO00022140A1 NUCLEIC ACID MOLECULES WHICH CODE A BRANCHING ENZYME FROM BACTERIA OF THE GENUS NEISSERIA, AND A METHOD FOR PRODUCING alpha -1,6-BRANCHED alpha -1,4-GLUCANS 审中-公开
标题翻译：核酸分子从该属奈瑟氏球菌，和方法的细菌编码分支酶用于生产α-1,6分支α-1,4-葡聚糖
公开(公告)号：WO00022140A1
公开(公告)日：2000-04-20
申请号：PCT/EP1999/007562
申请日：1999-10-08
IPC分类号： A01H5/00 , C07K16/40 , C12N1/15 , C12N1/19 , C12N1/21 , C12N5/10 , C12N9/10 , C12N15/09 , C12N15/54 , C12P19/04 , C12P19/16 , C12P19/20 , C07K16/12 , C08B30/00 , C12N15/63 , C12P21/02
CPC分类号： C12N9/1051 , C07K14/22 , C12N9/107 , C12P19/04 , C12P19/16 , C12P19/20
摘要： The invention relates to nucleic acid molecules which code a branching enzyme from a bacterium of the genus Neisseria, to vectors, host cells, plant cells and plants containing such nucleic acid molecules, as well as to starch which can be obtained from said plants. The invention also relates to an in-vitro method for producing alpha -1,6-branched alpha -1,4-glucans based on saccharose and an enzyme combination comprised of an amylosucrase and of a branching enzyme. In addition, the invention relates to the alpha -1,6-branched alpha -1,4-glucans which can be obtained using the method.
摘要翻译：描述的是编码来自奈瑟氏球菌属的细菌分支酶的核酸分子，载体，宿主细胞，植物细胞和植物，包括来自所描述的植物淀粉这样的核酸分子，以及获得。还公开了用于蔗糖的基础和淀粉蔗糖酶的酶组合和分支酶上产生α-1,6支化的α-1,4-葡聚糖的体外处理，并且α-1,6的方法获得支链的α-1,4-葡聚糖。

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