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1. 发明申请

WO2015050744A2 NEUROGENESIS SCREENING METHOD AND USES THEREOF 审中-公开
标题翻译：神经发生筛查方法及其用途
公开(公告)号：WO2015050744A2
公开(公告)日：2015-04-09
申请号：PCT/US2014/057004
申请日：2014-09-23
申请人： MJN U.S. HOLDINGS LLC
发明人： KUANG, Chenzhong, , XIAO, Yan, , HONDMANN, Dirk, , POELS, Eduard, K., , JOUNI, Zeina,
IPC分类号： G01N33/92
CPC分类号： G01N33/92 , A61K35/28 , G01N33/5026 , G01N33/5073
摘要： Provided herein are methods for detecting and/or confirming the neurogenesis effect of cholesterol, at least one oxysterol, EPA, EGCG and/or resveratrol utilizing adipose-derived stem cells (ADSCs). Further provided are methods of promoting neurogenesis in ADSCs. Also provided are methods for the use of cholesterol, an oxysterol, EPA, EGCG and/or resveratrol for the production of a neurologic component that may be incorporated into a nutritional composition for promoting neurogenesis in a pediatric subject.
摘要翻译：本文提供了用于检测和/或确认使用脂肪来源的干细胞（ADSC）的胆固醇，至少一种氧固醇，EPA，EGCG和/或白藜芦醇的神经发生作用的方法。还提供了促进ADSC中神经发生的方法。还提供了使用胆固醇，氧固醇，EPA，EGCG和/或白藜芦醇来生产可并入营养组合物中以促进儿科受试者的神经发生的神经学成分的方法。

2. 发明申请

WO2014185661A1 ３차원 콜라겐 겔 환경 내에서 배양된 세포를 이용한 암 세포의 전이성 모니터링 방법 审中-公开
标题翻译：使用三维胶原环境培养的细胞监测癌细胞分化的方法
公开(公告)号：WO2014185661A1
公开(公告)日：2014-11-20
申请号：PCT/KR2014/004146
申请日：2014-05-09
申请人： 재단법인 의약바이오컨버젼스연구단
发明人： 이정원 , 김성훈 , 이미숙
IPC分类号： C12Q1/04 , C12N5/09 , C12M1/34
CPC分类号： G01N33/574 , C12N5/0693 , C12N2501/727 , C12N2503/02 , C12N2533/54 , G01N33/5011 , G01N33/5023 , G01N33/5026 , G01N33/5029 , G01N33/57415 , G01N2333/4703 , G01N2333/4704 , G01N2333/4706 , G01N2440/14 , G01N2500/10
摘要： 본 발명은 3차원적 환경에서 배양된 암 세포의 모양관찰, 인바도포디아(invadopodia) 형성 및 전이에 관련된 단백질의 활성, 발현 및 발현위치 변화, 세포외기질의 분해 측정을 이용한 암 세포의 이동, 침윤 및 전이성 모니터링, 및 암 전이억제제 스크리닝 방법에 관한 것으로, 보다 구체적으로 3차원 콜라겐 겔 환경 내에서 배양된 세포에서 c-Jun 인산화의 감소가 snail1의 발현 증가 및 코르탁틴(cortactin) 발현 감소를 유발하였고, 이러한 단백질들간의 발현조절 관계는 환자로부터 수득한 유방암 세포조직에서도 동일함을 확인하였다. 또한, 3차원적 콜라겐 겔 환경 내의 유방암 세포에 JNK 억제제를 처리하였을 때, 세포의 모양이 길어지고, 세포 및 세포외 기질의 접촉부분이 밋밋해지고 가늘어지며, 암세포의 이동이 감소하고, 단백질 발현 변화에 있어서는 TGFβ1의 발현증가, smad2 및 smad3의 발현 및 활성 증가, snail1의 발현 증가 및 코르탁틴의 발현 감소 및 이에 따른 인바도포디아의 형성 저하를 확인하였다. 또한, 3차원 콜라겐 겔 환경에서 코르탁틴외에도 MT1-MMP가 다른 인바도포디아 마커로 사용될 수 있고, 이는 JNK 억제가 코르탁틴의 발현을 감소시키고, snail1의 발현을 증가시키면서 동시에 MT1-MMP의 위치 및 역할에 부정적 영항을 미쳐 인바도포디아의 형성을 억제하며, 콜라겐 겔 기질의 분해능을 억제하는 것을 확인함으로써 암 세포의 이동, 침윤, 전이성 및 전이 정도를 모니터링하는 방법 및 암 전이억제제의 스크리닝 방법으로 사용할 수 있고, 이는 약물 개발에 필요한 전임상 실험 시 저비용 고효율의 부가가치를 창출할 수 있는 스크리닝 방법 중의 하나로 유용하게 사용될 것이다.
摘要翻译：本发明涉及通过观察在三维环境中培养的癌细胞的形状来监测癌细胞的迁移，侵袭和转移的方法，并且测量与侵入体形成相关的蛋白质的表达位点的活性，表达和变化和转移，以及细胞外基质的降解; 涉及筛选癌症转移抑制剂的方法。更具体地说，证实了c-Jun磷酸化的减少诱导了在三维环境中培养的细胞中蜗牛1的增加和皮质醇表达的降低，并且蛋白质之间的表达调节关系与乳腺癌中的相同从患者获得的组织。此外，证实当用JNK抑制剂处理三维胶原凝胶环境中的乳腺癌细胞时，细胞的形状变长; 细胞和细胞外基质的接触区域变平且变薄; 癌细胞迁移减少; 观察到蛋白质表达的变化，如TGF-bgr1表达增加，smad2和smad3表达和活性的增加，snail1表达的增加，cortactin表达的降低以及由此造成的invadopodia形成的减少。另外，在三维胶原凝胶环境中，除了皮质激素外，MT1-MMP可以作为侵袭过程的标记，证实JNK的抑制作用导致皮质激素表达的降低和snail1表达的增加，严重影响MT1-MMP的抑制形成侵袭性的位点和作用，抑制胶原凝胶底物的降解活性。因此，本发明可以用作监测癌细胞的迁移，侵袭，转移和转移程度的方法和用于筛选癌症转移抑制剂的方法，并且将作为能够产生低分子量的筛选方法之一 - 药物开发所需的临床前试验时的高效附加值。

3. 发明申请

WO2014182897A2 COMPOSITIONS AND METHODS FOR THE PRODUCTION OF GLUTEN FREE FOOD PRODUCTS 审中-公开
标题翻译：用于生产无糖食品的组合物和方法
公开(公告)号：WO2014182897A2
公开(公告)日：2014-11-13
申请号：PCT/US2014/037296
申请日：2014-05-08
申请人： JM BIOLOGICALS
发明人： MESSING, Joachim , CICLITIRA, Paul, J.
IPC分类号： A01H5/00 , C12N15/82
CPC分类号： C12N15/8251 , A23L7/198 , A23V2002/00 , C07K14/415 , C12N15/8218 , C12N15/8257 , G01N33/5026 , G01N33/505 , G01N33/5097
摘要： Compositions and methods for the production of baked goods and flour, which do not induce CD are disclosed.
摘要翻译：公开了不诱导CD的焙烤食品和面粉生产的组合物和方法。

4. 发明申请

WO2014168788A1 BIOLOGICAL SPECIMEN EVALUATION METHODS USING CYTOLOGY AND IMMUNOLOGY 审中-公开
标题翻译：使用细胞学和免疫学的生物样本评估方法
公开(公告)号：WO2014168788A1
公开(公告)日：2014-10-16
申请号：PCT/US2014/032563
申请日：2014-04-01
申请人： CYTOCORE, INC.
发明人： JOLLEY, Michael E. , DOMANIK, Richard A.
IPC分类号： G01N33/50
CPC分类号： G01N33/5091 , G01N33/5023 , G01N33/5026 , G01N33/57496 , G01N33/6869 , G01N2333/525 , G01N2333/5428 , G01N2333/715 , G01N2800/50 , G01N2800/7028 , G01N2800/7095
摘要： Information on cytokines and cytology obtained from a biological specimen are combined as a method of predicting the risk that dysplasia will progress to cancer.Methods are disclosed herein to augment the evaluation of biological samples from subjects being tested for cancer. In addition to the cell types that are traditionally considered in the morphology-based cytological screening of specimens, methods disclosed herein add evaluations of certain cell types and cytokines that are traditionally discounted or ignored during screening.
摘要翻译：将从生物标本获得的关于细胞因子和细胞学的信息合并为预测发育不良将进展为癌症的风险的方法。本文公开了增加对被测试癌症的受试者的生物样品的评价的方法。除了在基于形态学的细胞学筛选标本中传统考虑的细胞类型之外，本文公开的方法增加了在筛选期间传统上折扣或忽略的某些细胞类型和细胞因子的评估。

5. 发明申请

WO2014033546A3 METHODS FOR PRODUCING CELLS HAVING A PHENOTYPE OF A PRIMARY HUMAN HEPATOCYTES AND COMPOSITIONS 审中-公开
标题翻译：生产具有原代人类肝细胞表型的细胞和组合物的方法
公开(公告)号：WO2014033546A3
公开(公告)日：2014-04-17
申请号：PCT/IB2013002501
申请日：2013-08-30
申请人： UNIV ALBERTA
发明人： TYRRELL LORNE D , STEENBERGEN HENDRIKJE GEESJE , JOYCE MICHAEL A
IPC分类号： C12N5/071 , C12N5/10 , C12N7/00 , C12Q1/02 , C12Q1/70 , G01N33/15 , G01N33/48
CPC分类号： C12N5/067 , C12N7/00 , C12N2500/84 , C12N2503/02 , C12N2506/30 , C12N2730/10152 , C12N2770/24252 , G01N33/5014 , G01N33/5026 , G01N33/5067
摘要： The present disclosure provides methods and compositions relating to in vitro cultures of human hepatocyte cell lines which exhibit a primary human hepatocyte phenotype. Such cell lines are susceptible to infection by a hepatotrophic virus, such as HCV or HBV, and support both viral replication and high levels of viral particle production. Such in vitro cultures find use in production and study of hepatotrophic virus, as well as methods of screening (e.g., for antiviral drugs, assessing drug metabolism), and study of primary human hepatocytes.
摘要翻译：本公开提供了涉及显示初级人肝细胞表型的人肝细胞系的体外培养物的方法和组合物。此类细胞系易受肝细胞病毒（如HCV或HBV）感染，并且支持病毒复制和高水平的病毒颗粒产生。这种体外培养物可用于肝营养型病毒的生产和研究，以及筛选（例如，抗病毒药物，评估药物代谢）和研究原代人肝细胞的方法。

6. 发明申请

WO2013188748A1 TECHNOLOGY FOR CELL TRANSDIFFERENTIATION 审中-公开
标题翻译：细胞转导技术
公开(公告)号：WO2013188748A1
公开(公告)日：2013-12-19
申请号：PCT/US2013/045848
申请日：2013-06-14
申请人： FLUIDIGM CORPORATION
发明人： LI, Nianzhen , UNGER, Marc
IPC分类号： C12N5/10 , C12N5/02 , C12Q1/68 , C12M3/02
CPC分类号： C12N5/0618 , B01L3/5027 , B01L2200/0647 , B01L2300/0816 , B01L2300/0829 , B01L2300/0864 , C12M23/16 , C12N5/0619 , C12N15/111 , C12N2310/141 , C12N2320/12 , C12N2501/65 , C12N2503/02 , C12N2506/02 , C12N2506/1307 , C12Q1/6881 , C12Q2600/158 , G01N33/5026 , G01N33/57438
摘要： This invention provides technology for transdifferentiating cells from one cell type to another. The cells are cultured with one or more vector-free gene regulator oligonucleotides concurrently or in succession, and then harvested when cell markers or the morphology of the culture shows that transdifferentiation is complete. Suitable gene regular oligonucleotides include microRNAs and messenger RNAs that encode a differentiation factor. Conditions for transdifferentiation can be optimized by dividing cells into different culture chambers of a microfluidic device. Cells are cultured with different additives in each chamber, and then compared. Transdifferentiated cells produced according to this invention can provide a consistent source of tissue for use in regenerative medicine.
摘要翻译：本发明提供了将细胞从一种细胞类型转移到另一种细胞类型的技术。细胞与一种或多种无载体的基因调节寡核苷酸同时或相继培养，然后当细胞标记物或培养物的形态显示转分化完成时收获细胞。合适的基因正常寡核苷酸包括编码分化因子的微小RNA和信使RNA。可以通过将细胞分成微流体装置的不同培养室来优化用于转分化的条件。每个室中用不同的添加剂培养细胞，然后进行比较。根据本发明生产的转分化细胞可提供用于再生医学的一致的组织来源。

7. 发明申请

WO2013135809A1 DEVICES AND METHODS FOR OBSERVING EUKARYOTIC CELLS WITHOUT CELL WALL 审中-公开
标题翻译：用于观察没有细胞壁的真核细胞的装置和方法
公开(公告)号：WO2013135809A1
公开(公告)日：2013-09-19
申请号：PCT/EP2013/055227
申请日：2013-03-14
申请人： CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE , UNIVERSITE DE STRASBOURG , INSERM (INSTITUT NATIONAL DE LA SANTÉ ET DE LA RECHERCHE MÉDICALE)
发明人： RIVELINE, Daniel , WOLLRAB, Viktoria
IPC分类号： C12M3/00 , G01N33/50
CPC分类号： G01N33/5005 , G01N33/5014 , G01N33/5023 , G01N33/5026 , G01N33/5091 , G01N2500/04 , G01N2500/10 , G01N2800/7023
摘要： The present invention relates to methods and devices for observing eukaryotic cells devoid of cell wall, in particular for observing the cytokinetic ring, the device comprising a plurality of wells suitable for containing only one single eukaryotic cell and characterized in that the dimensions of the wells constrain the cells into an oblong shape with a long axis parallel to the depth of the wells.
摘要翻译：本发明涉及用于观察没有细胞壁的真核细胞的方法和装置，特别是用于观察细胞运动环的装置，该装置包括多个适于仅含有一个单个真核细胞的孔，其特征在于，孔的尺寸约束细胞成长椭圆形，长轴平行于孔的深度。

8. 发明申请

WO2013056253A1 HIGH THROUGHPUT INSTRUMENTATION TO SCREEN CELLS AND PARTICLES BASED ON THEIR MECHANICAL PROPERTIES 审中-公开
标题翻译：基于其机械性能的高电导率仪器筛选细胞和颗粒
公开(公告)号：WO2013056253A1
公开(公告)日：2013-04-18
申请号：PCT/US2012/060297
申请日：2012-10-15
申请人： THE REGENTS OF THE UNIVERSITY OF CALIFORNIA
发明人： ROWAT, Amy , QI, Dongping
IPC分类号： C12Q1/24 , C12Q1/02 , B07B1/00
CPC分类号： G01N33/5091 , B07B1/46 , B07B13/04 , G01N33/5005 , G01N33/5011 , G01N33/5026 , G01N2500/10
摘要： A system for the high-throughput screening of mechanical properties of cells and/or particles is provided. In certain embodiments the system comprises a first structure comprising a plurality of sample receiving wells; a second structure comprising a plurality of exit ports; a porous structure disposed between said plurality of sample receiving wells and said plurality of exit ports and in communication with said plurality of sample receiving wells and in communication with said plurality of exit ports, and said first structure, said second structure and said porous structure are sealed or configured such that when sufficient pressure is applied to sample receiving wells in said first structure said cells or particles migrate through the porous structure into said exit ports, and wherein the mean or the median pore size of said porous structure is smaller than the mean or median cross-section of a cell or particle that is to be screened.
摘要翻译：提供了用于高通量筛选细胞和/或颗粒的机械性能的系统。在某些实施例中，系统包括包括多个样品接收孔的第一结构; 第二结构，包括多个出口; 设置在所述多个样品接收孔和所述多个出口之间并与所述多个样品接收阱连通并与所述多个出口连通的多孔结构，所述第一结构，所述第二结构和所述多孔结构是密封或构造使得当对所述第一结构中的样品接收孔施加足够的压力时，所述细胞或颗粒通过多孔结构迁移到所述出口中，并且其中所述多孔结构的平均孔径或中值孔径小于平均值或要筛选的细胞或颗粒的中间截面。

9. 发明申请

WO2013019984A1 RAPID, MASSIVELY PARALLEL SINGLE-CELL DRUG RESPONSE MEASUREMENTS VIA LIVE CELL INTERFEROMETRY 审中-公开
标题翻译：快速，大规模并行单细胞药物反应测量通过活细胞干扰
公开(公告)号：WO2013019984A1
公开(公告)日：2013-02-07
申请号：PCT/US2012/049388
申请日：2012-08-02
申请人： THE REGENTS OF THE UNIVERSITY OF CALIFORNIA , REED, Jason C. , TEITELL, Michael A.
发明人： REED, Jason C. , TEITELL, Michael A.
IPC分类号： G01B9/02 , G01N33/483
CPC分类号： G01N33/57492 , G01B9/02057 , G01B9/02089 , G01B9/0209 , G01B11/0675 , G01N33/502 , G01N33/5026
摘要： A central question in cancer therapy is how individual cells within a population of tumor cells respond to drugs designed to arrest their growth. However, the absolute growth of cells, their change in physical mass, whether cancerous or physiologic, is difficult to measure directly with traditional techniques. Embodiments of the invention provide live cell interferometry (LCI) for rapid, realtime quantification of cell mass in cells exposed to a changing environment. Overall, LCI provides a conceptual advance for assessing cell populations to identify, monitor, and measure single cell responses, such as to therapeutic drugs.
摘要翻译：癌症治疗中的一个核心问题是肿瘤细胞群体内的单个细胞如何响应旨在阻止其生长的药物。然而，传统技术难以直接测量细胞的绝对生长，物理量的变化，无论是癌性还是生理学上的变化。本发明的实施例提供了用于在暴露于不断变化的环境中的细胞中快速，实时定量细胞质量的活细胞干涉测量（LCI）。总体而言，LCI提供了评估细胞群体以鉴别，监测和测量单细胞反应（如治疗药物）的概念性进展。

10. 发明申请

WO2013006828A1 METHOD OF ANALYZING CARDIOVASCULAR DISORDERS AND USES THEREOF 审中-公开
标题翻译：分析心血管疾病的方法及其用途
公开(公告)号：WO2013006828A1
公开(公告)日：2013-01-10
申请号：PCT/US2012/045832
申请日：2012-07-06
申请人： SCRIPPS HEALTH , VERIDEX LLC , TOPOL, Eric , DAMANI, Samir , CONNELLY, Mark , RAO, Galla, Chandra
发明人： TOPOL, Eric , DAMANI, Samir , CONNELLY, Mark , RAO, Galla, Chandra
IPC分类号： G01N33/50 , G01N1/28
CPC分类号： C12Q1/04 , G01N15/1475 , G01N33/5026 , G01N33/5064 , G01N2800/32 , G01N2800/52
摘要： Compositions, systems, and methods comprising circulating endothelial cells (CECs) are provided. The compositions described herein utilize isolated CECs, including compositions in a form that allows analysis of the CECs. The systems described herein utilize isolated CECs and an analytical tool or an output from an analytical tool. The methods described herein are related to the use of isolated CECs and analytical tools for providing information, to a health care provider or the CEC donor, that is relevant to the cardiovascular health of the CEC donor. Thus, the compositions, systems and methods described herein involve both a transformation (e.g., non-isolated CECs to isolated CECs, or isolated CECs to analyzed CECs) and a machine (e.g., isolation tools and analytical tools).
摘要翻译：提供了包含循环内皮细胞（CEC）的组合物，系统和方法。本文描述的组合物使用分离的CEC，包括允许分析CEC的形式的组合物。本文描述的系统使用分离的CEC和分析工具或来自分析工具的输出。本文描述的方法涉及使用分离的CEC和分析工具来向医疗保健提供者或CEC供体提供与CEC供体的心血管健康相关的信息。因此，本文所述的组合物，系统和方法涉及转化（例如，分离的CEC与分离的CEC的分离的CEC或分离的CEC的分离的CEC）和机器（例如，隔离工具和分析工具）。

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