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1. 发明申请

WO2015181310A3 ITACONIC ACID AND ITACONATE METHYLESTER AND DIMETHYLESTER PRODUCTION 审中-公开
标题翻译：异柠檬酸和甲基丙烯酸甲酯和二甲醚生产
公开(公告)号：WO2015181310A3
公开(公告)日：2016-02-18
申请号：PCT/EP2015061882
申请日：2015-05-28
申请人： DSM IP ASSETS BV
发明人： ZHAO ZHENG , MEIJRINK BERNARD , HOEVEN VAN DER ROBERTUS ANTONIUS MIJNDERT , WU LIANG , ROUBOS JOHANNES ANDRIES
IPC分类号： C12N15/63 , C12P1/00
CPC分类号： C12P7/44 , C07K14/395 , C12N9/0008 , C12N9/1025 , C12N9/1029 , C12N9/1217 , C12N9/88 , C12N9/93 , C12N15/52 , C12P7/62 , C12Y203/03001 , C12Y401/01006 , C12Y401/01031 , C12Y401/01032 , C12Y604/01001
摘要： The present invention relates to a recombinant yeast cell which is capable of producing one or more of 4-methyl itaconate, 1-methyl itaconate or 1,4-dimethyl itaconate. The invention also relates to a recombinant yeast cell which is capable of producing itaconic acid and which overexpresses: - a nucleic acid encoding a polypeptide having cis-aconitate decarboxylase activity; and - a nucleic acid encoding a polypeptide which catalyzes a reaction towards acetyl CoA. These recombinant yeast cells may be used in processes for the production of itaconic acid, 4-methyl itaconate, 1-methyl itaconate or 1,4-dimethyl itaconate.
摘要翻译：本发明涉及能够产生衣康酸4-甲酯，衣康酸1-甲酯或衣康酸1,4-二甲酯中的一种或多种的重组酵母细胞。本发明还涉及能够产生衣康酸并且过表达的重组酵母细胞： - 编码具有顺 - 乌头酸脱羧酶活性的多肽的核酸; 以及 - 编码催化向乙酰辅酶A反应的多肽的核酸。这些重组酵母细胞可用于生产衣康酸，衣康酸4-甲酯，衣康酸1-甲酯或衣康酸1,4-二甲酯的方法。

2. 发明申请

WO2015155791A2 PRODUCTION OF SUCCINIC ACID FROM ORGANIC WASTE OR BIOGAS OR METHANE USING RECOMBINANT METHANOTROPHIC BACTERIUM 审中-公开
标题翻译：从有机废物或生物或甲烷使用重组甲烷生物细菌生产己酸
公开(公告)号：WO2015155791A2
公开(公告)日：2015-10-15
申请号：PCT/IN2015/000169
申请日：2015-04-13
申请人： STRING BIO PRIVATE LIMITED
发明人： SUBBIAN, Ezhilkani
IPC分类号： C12P7/46 , C12N1/21
CPC分类号： C12P7/46 , C12N9/0006 , C12N9/88 , C12N9/93 , C12N15/52 , C12N15/74 , C12Y101/01037 , C12Y401/01031 , C12Y604/01001 , Y02E50/343
摘要： The present disclosure provides for production of succinic acid from organic waste or biogas or methane using recombinant methanotrophic bacterium. In one embodiment, the recombinant methanotrophic bacterium includes exogenous nucleic acid(s) or gene(s) encoding for specified enzymes. In a further embodiment, succinic acid producing capacity of the recombinant methanotrophic bacterium is increased above the basal level by overexpression or/and down-regulation of selected gene(s). In another embodiment, a process of producing succinic acid using the recombinant methanotrophic bacterium is disclosed. The present invention successfully solves the problems in converting organic waste to a useful chemical thereby providing an environment-friendly and commercially viable solution for waste management.
摘要翻译：本公开提供了使用重组甲烷营养细菌从有机废物或沼气或甲烷生产琥珀酸。在一个实施方案中，重组甲烷营养细菌包括编码特定酶的外源核酸或基因。在另一个实施方案中，重组甲烷营养细菌的琥珀酸产生能力通过过表达和/或下调所选择的基因而增加到基础水平以上。在另一个实施方案中，公开了使用重组甲烷营养细菌生产琥珀酸的方法。本发明成功地解决了将有机废物转化为有用化学品的问题，从而为废物管理提供了环境友好且商业上可行的解决方案。

3. 发明申请

WO2015100338A3 METHODS AND ORGANISMS WITH INCREASED CARBON FLUX EFFICIENCIES 审中-公开
标题翻译：具有增加的碳通量效率的方法和生物
公开(公告)号：WO2015100338A3
公开(公告)日：2015-09-03
申请号：PCT/US2014072178
申请日：2014-12-23
申请人： GENOMATICA INC
发明人： BURGARD ANTHONY P , OSTERHOUT ROBIN E , VAN DIEN STEPHEN J , PHARKYA PRITI , YANG TAE HOON , CHOI JUNGIK
IPC分类号： C12N1/21 , C12P1/04 , C12P7/16 , C12P7/52
CPC分类号： C12P7/18 , C07K14/245 , C12N1/20 , C12N9/0036 , C12N9/0057 , C12N9/1205 , C12N9/16 , C12N9/52 , C12N9/88 , C12N9/93 , C12N15/52 , C12P7/42 , C12Y106/01002 , C12Y110/03 , C12Y207/0104 , C12Y301/00 , C12Y301/02 , C12Y301/02002 , C12Y304/21092 , C12Y401/01031 , C12Y401/01032
摘要： The invention is directed to a non-naturally occurring microbial organism comprising a first attenuation of a succinyl-CoA synthetase or transferase and at least a second attenuation of a succinyl-CoA converting enzyme or a gene encoding a succinate producing enzyme within a multi-step pathway having a net conversion of succinyl-CoA to succinate.
摘要翻译：本发明涉及非天然存在的微生物生物体，其包含琥珀酰-CoA合成酶或转移酶的第一次减毒以及多步骤中琥珀酰-CoA转化酶或编码琥珀酸生产酶的基因的至少第二次减毒具有琥珀酰-CoA到琥珀酸的净转化的途径。

4. 发明申请

WO2014018902A2 METHODS AND COMPOSITIONS FOR THE AUGMENTATION OF PYRUVATE AND ACETYL-COA FORMATION 审中-公开
标题翻译：增加丙酮酸和乙酰-CoA形成的方法和组合物
公开(公告)号：WO2014018902A2
公开(公告)日：2014-01-30
申请号：PCT/US2013052346
申请日：2013-07-26
申请人： JOULE UNLTD TECHNOLOGIES INC
发明人： SKRALY FRANK A
IPC分类号： C12P7/08 , C12N1/21
CPC分类号： C12N9/0006 , C12N9/0008 , C12N9/88 , C12P7/065 , C12P7/40 , C12P19/40 , C12Y101/01037 , C12Y101/0104 , C12Y101/01082 , C12Y102/04001 , C12Y102/07005 , C12Y401/01003 , C12Y401/01031 , Y02E50/17
摘要： The present disclosure identifies methods and compositions for modifying photoautotrophic organisms as hosts, such that the organisms efficiently convert carbon dioxide and light into pyruvate or acetyl-CoA, and in particular the use of such organisms for the commercial production of molecules derived from these precursors, e.g., ethanol.
摘要翻译：本公开鉴定用于修饰光自养生物作为宿主的方法和组合物，使得所述生物将二氧化碳和光有效地转化为丙酮酸或乙酰-CoA，并且特别是使用这些生物用于商业生产源自这些前体的分子，如乙醇。

5. 发明申请

WO2013063487A1 METHODS AND COMPOSITIONS FOR SILENCING GENES USING ARTIFICIAL MICRORNAS 审中-公开
标题翻译：使用人造微生物沉默基因的方法和组合物
公开(公告)号：WO2013063487A1
公开(公告)日：2013-05-02
申请号：PCT/US2012/062249
申请日：2012-10-26
申请人： E. I. DU PONT DE NEMOURS AND COMPANY , PIONEER HI-BRED INTERNATIONAL
发明人： KUREK, Itzhak , MCGONIGLE, Brian , ZHU, Genhai
IPC分类号： C12N15/63 , C12N15/82
CPC分类号： C07K14/415 , C12N9/14 , C12N9/88 , C12N15/8218 , C12N15/8269 , C12Y306/01 , C12Y401/01031
摘要： Methods and compositions are provided that employ microRNA (miRNA) that, when expressed in a plant cell, is capable of reducing the level of mRNA of a target sequence (i.e. endogenous sequence) without reducing the level of mRNA of one or more closely related sequences. While miRNAs can be designed with specificity for a particular target sequence, the instant application demonstrates that a miRNA can specifically silence a target sequence without silencing a closely related sequence having high sequence identity to the target sequence. In certain embodiments, an endogenous target sequence can be suppressed with a recombinant miRNA expression construct without silencing a recombinant polynucleotide of interest having a sequence closely related to the target sequence. Such methods and compositions employ recombinant miRNA expression constructs which produce a 21-nt miRNA. Transgenic plant cells, plants and seeds incorporating miRNA expression constructs and recombinant polynucleotide constructs comprising polynucleotides of interest are also provided.
摘要翻译：提供了使用微小RNA（miRNA）的方法和组合物，其在植物细胞中表达时能够降低靶序列（即内源序列）的mRNA水平而不降低一个或多个密切相关序列的mRNA水平。虽然miRNA可以针对特定靶序列被设计为特异性，但是本申请表明miRNA可以特异性沉默靶序列而不沉默与靶序列具有高序列同一性的紧密相关的序列。在某些实施方案中，可以用重组miRNA表达构建体抑制内源靶序列，而不沉默具有与靶序列密切相关的序列的目标重组多核苷酸。这些方法和组合物使用产生21-nt miRNA的重组miRNA表达构建体。还提供了转基因植物细胞，植物和掺入miRNA表达构建体的种子和包含感兴趣的多核苷酸的重组多核苷酸构建体。

6. 发明申请

WO2018126610A1 利用木糖诱导产生四氢嘧啶的基因工程菌及其应用审中-公开
公开(公告)号：WO2018126610A1
公开(公告)日：2018-07-12
申请号：PCT/CN2017/088284
申请日：2017-06-14
申请人：天津科技大学
发明人：谢希贤 , 吴雪娇 , 陈宁 , 鄢芳清 , 马倩 , 麻杰 , 张红超
IPC分类号： C12N1/21 , C12P17/12 , C12R1/19
CPC分类号： C12N9/0006 , C12N9/1029 , C12N9/1096 , C12N9/1217 , C12N9/127 , C12N9/88 , C12P17/12 , C12Y101/01003 , C12Y203/01178 , C12Y206/01076 , C12Y207/02004 , C12Y207/07048 , C12Y401/01031 , C12Y402/01108
摘要：一种利用木糖诱导产生四氢嘧啶的基因工程菌及其构建方法与应用。所述菌株是在大肠杆菌染色体上异源表达来自伸长盐单胞菌中的ectABC基因簇，重构四氢嘧啶合成通路，构建无质粒系统，利用木糖转运蛋白编码基因的启动子控制来源于T7噬菌体的RNA聚合酶，同时结合T7强启动子系统启动目的基因高效表达，从而达到摇瓶发酵20-28h后四氢嘧啶的产量达到了12-16g/L，5L发酵罐发酵24-40h后四氢嘧啶的产量达到了35-50g/L的效果。

7. 发明申请

WO2015181310A2 ITACONIC ACID AND ITACONATE METHYLESTER AND DIMETHYLESTER PRODUCTION 审中-公开
标题翻译：异柠檬酸和甲基丙烯酸甲酯和二甲醚生产
公开(公告)号：WO2015181310A2
公开(公告)日：2015-12-03
申请号：PCT/EP2015/061882
申请日：2015-05-28
申请人： DSM IP ASSETS B.V.
发明人： ZHAO, Zheng , MEIJRINK, Bernard , HOEVEN, VAN DER, Robertus Antonius Mijndert , WU, Liang , ROUBOS, Johannes Andries
IPC分类号： C12N15/63
CPC分类号： C12P7/44 , C07K14/395 , C12N9/0008 , C12N9/1025 , C12N9/1029 , C12N9/1217 , C12N9/88 , C12N9/93 , C12N15/52 , C12P7/62 , C12Y203/03001 , C12Y401/01006 , C12Y401/01031 , C12Y401/01032 , C12Y604/01001
摘要： The present invention relates to a recombinant yeast cell which is capable of producing one or more of 4-methyl itaconate, 1-methyl itaconate or 1,4-dimethyl itaconate. The invention also relates to a recombinant yeast cell which is capable of producing itaconic acid and which overexpresses: - a nucleic acid encoding a polypeptide having cis-aconitate decarboxylase activity; and - a nucleic acid encoding a polypeptide which catalyzes a reaction towards acetyl CoA. These recombinant yeast cells may be used in processes for the production of itaconic acid, 4-methyl itaconate, 1-methyl itaconate or 1,4-dimethyl itaconate.
摘要翻译：本发明涉及能够产生衣康酸4-甲酯，衣康酸1-甲酯或衣康酸1,4-二甲酯中的一种或多种的重组酵母细胞。本发明还涉及能够产生衣康酸并且过表达的重组酵母细胞： - 编码具有顺 - 乌头酸脱羧酶活性的多肽的核酸; 以及 - 编码催化向乙酰辅酶A反应的多肽的核酸。这些重组酵母细胞可用于生产衣康酸，衣康酸4-甲酯，衣康酸1-甲酯或衣康酸1,4-二甲酯的方法。

8. 发明申请

WO2015100338A2 METHODS AND ORGANISMS WITH INCREASED CARBON FLUX EFFICIENCIES 审中-公开
标题翻译：具有增加碳通量效率的方法和有机体
公开(公告)号：WO2015100338A2
公开(公告)日：2015-07-02
申请号：PCT/US2014/072178
申请日：2014-12-23
申请人： GENOMATICA, INC.
发明人： BURGARD, Anthony, P. , OSTERHOUT, Robin, E. , VAN DIEN, Stephen, J. , PHARKYA, Priti , YANG, Tae, Hoon , CHOI, Jungik
IPC分类号： C12N1/21
CPC分类号： C12P7/18 , C07K14/245 , C12N1/20 , C12N9/0036 , C12N9/0057 , C12N9/1205 , C12N9/16 , C12N9/52 , C12N9/88 , C12N9/93 , C12N15/52 , C12P7/42 , C12Y106/01002 , C12Y110/03 , C12Y207/0104 , C12Y301/00 , C12Y301/02 , C12Y301/02002 , C12Y304/21092 , C12Y401/01031 , C12Y401/01032
摘要： The invention is directed to a non-naturally occurring microbial organism comprising a first attenuation of a succinyl-CoA synthetase or transferase and at least a second attenuation of a succinyl-CoA converting enzyme or a gene encoding a succinate producing enzyme within a multi-step pathway having a net conversion of succinyl-CoA to succinate.
摘要翻译：本发明涉及一种非天然存在的微生物生物体，其包含琥珀酰-CoA合成酶或转移酶的第一次衰减，以及琥珀酰-CoA转化酶或编码多步骤中产琥珀酸酶的基因的至少第二次衰减途径具有琥珀酰-CoA向琥珀酸盐的净转化。

9. 发明申请

WO2014062564A1 MICROORGANISMS AND METHODS FOR PRODUCTION OF SPECIFIC LENGTH FATTY ALCOHOLS AND RELATED COMPOUNDS 审中-公开
标题翻译：用于生产特定长度脂肪醇及相关化合物的微生物和方法
公开(公告)号：WO2014062564A1
公开(公告)日：2014-04-24
申请号：PCT/US2013/064827
申请日：2013-10-14
申请人： GENOMATICA, INC.
发明人： OSTERHOUT, Robin, E. , BURGARD, Anthony, P.
IPC分类号： C12P7/04 , C12P7/40 , C12P7/64 , C07C53/00 , C07C57/00 , C07C29/00 , C07C31/02 , C07C33/00 , C07C47/263 , C12N1/00
CPC分类号： C12P7/64 , C07C31/207 , C12N9/0006 , C12N9/0008 , C12N9/001 , C12N9/1025 , C12N9/1029 , C12N9/1217 , C12N9/1294 , C12N9/13 , C12N9/16 , C12N9/88 , C12N9/93 , C12N15/52 , C12P7/04 , C12P7/24 , C12P7/6409 , C12Y101/01035 , C12Y101/01037 , C12Y101/05004 , C12Y101/05006 , C12Y101/99033 , C12Y102/01 , C12Y102/01002 , C12Y102/01003 , C12Y102/0101 , C12Y102/01015 , C12Y102/01018 , C12Y102/01027 , C12Y102/01042 , C12Y102/0105 , C12Y102/01076 , C12Y102/05001 , C12Y103/01044 , C12Y103/99012 , C12Y203/01008 , C12Y203/01016 , C12Y203/01054 , C12Y203/01174 , C12Y203/03008 , C12Y207/02001 , C12Y207/02007 , C12Y207/09002 , C12Y208/03003 , C12Y301/03001 , C12Y301/03002 , C12Y301/0302 , C12Y301/0306 , C12Y401/01 , C12Y401/01001 , C12Y401/01003 , C12Y401/01007 , C12Y401/01009 , C12Y401/01031 , C12Y401/01032 , C12Y401/01049 , C12Y401/01074 , C12Y401/01082 , C12Y401/02005 , C12Y401/03006 , C12Y401/03034 , C12Y402/01017 , C12Y602/01001 , C12Y602/01013 , C12Y602/01018 , C12Y604/01001
摘要： The invention provides non-naturally occurring microbial organisms containing a fatty alcohol, fatty aldehyde or fatty acid pathway, wherein the microbial organisms selectively produce a fatty alcohol, fatty aldehyde or fatty acid of a specified length. Also provided are non-naturally occurring microbial organisms having a fatty alcohol, fatty aldehyde or fatty acid pathway, wherein the microbial organisms further include an acetyl-CoA pathway. In some aspects, the microbial organisms of the invention have select gene disruptions or enzyme attenuations that increase production of fatty alcohols, fatty aldehydes or fatty acids. The invention additionally provides methods of using the above microbial organisms to produce a fatty alcohol, a fatty aldehyde or a fatty acid.
摘要翻译：本发明提供了包含脂肪醇，脂肪醛或脂肪酸途径的非天然存在的微生物，其中微生物有选择地产生一定长度的脂肪醇，脂肪醛或脂肪酸。还提供了具有脂肪醇，脂肪醛或脂肪酸途径的非天然存在的微生物，其中所述微生物进一步包括乙酰辅酶A途径。在一些方面，本发明的微生物有选择性的基因破坏或酶减弱，其增加脂肪醇，脂肪醛或脂肪酸的产生。本发明另外提供使用上述微生物生产脂肪醇，脂肪醛或脂肪酸的方法。

10. 发明申请

WO2011099006A2 ENZYMATIC SYSTEMS FOR CARBON FIXATION AND METHODS OF GENERATING SAME 审中-公开
标题翻译：碳固定的ENZYMATIC系统及其生成方法
公开(公告)号：WO2011099006A2
公开(公告)日：2011-08-18
申请号：PCT/IL2011/000145
申请日：2011-02-10
申请人： YEDA RESEARCH AND DEVELOPMENT CO. LTD. , MILO, Ron , BAR-EVEN, Arren , NOOR, Elad
发明人： MILO, Ron , BAR-EVEN, Arren , NOOR, Elad
IPC分类号： C12N15/82 , C12N9/00 , C12N9/88 , C12N9/02 , C12N9/04 , C12N9/10 , C12N9/18 , C12N9/90 , G06F19/12
CPC分类号： C12N9/93 , C12N9/0006 , C12N9/0008 , C12N9/1018 , C12N9/1205 , C12N9/13 , C12N9/18 , C12N9/88 , C12N9/90 , C12N15/70 , C12N15/8245 , C12N15/8247 , C12N15/8269 , C12P7/40 , C12P7/42 , C12P7/46 , C12P11/00 , C12Y101/01059 , C12Y102/01018 , C12Y201/03001 , C12Y208/03001 , C12Y401/01031 , C12Y402/01017 , C12Y402/01054 , C12Y604/01001 , C12Y604/01002 , G06F19/12 , Y02P20/52
摘要： A system for carbon fixation is provided. The system comprises enzymes which catalyze reactions of a carbon fixation pathway, wherein at least one of the reactions of the carbon fixation pathway is a carboxylation reaction, wherein products of the reactions of the carbon fixation pathway comprise oxaloacetate and malonyl-CoA, wherein an enzyme which performs the carboxylation reaction is selected from the group consisting of phophoenolpyruvate (PEP) carboxlase, pyruvate carboxylase and acetyl-CoA carboxylase and wherein an export product of the carbon fixation pathway is glyoxylate. Additional carbon fixation pathways are also provided and methods of generating same.
摘要翻译：提供了一种用于碳固定的系统。该系统包含催化碳固定途径的反应的酶，其中碳固定途径的至少一个反应是羧化反应，其中碳固定途径的反应产物包含草酰乙酸和丙二酰-CoA，其中酶其中进行羧化反应的酶选自磷酸烯醇式丙酮酸（PEP）羧化酶，丙酮酸羧化酶和乙酰-CoA羧化酶，并且其中碳固定途径的出口产物是乙醛酸盐。还提供了额外的碳固定途径和产生同样的方法。

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