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    • 10. 发明申请
    • COMPOSITIONS AND METHODS FOR THE BIOSYNTHESIS OF 1,4-BUTANEDIOL AND ITS PRECURSORS
    • 1,4-丁二醇及其前体的生物合成的组合物和方法
    • WO2008115840A2
    • 2008-09-25
    • PCT/US2008/057168
    • 2008-03-14
    • GENOMATICA, INC.BURK, Mark, J.VAN DIEN, Stephen, J.BURGARD, AnthonyNIU, Wei
    • BURK, Mark, J.VAN DIEN, Stephen, J.BURGARD, AnthonyNIU, Wei
    • C12N1/21
    • C12N15/52B01D3/002C12N9/0006C12N9/0008C12N9/88C12N9/93C12N15/70C12N15/81C12P7/18C12P7/42C12P7/52C12P17/04C12Y101/01061C12Y102/01076C12Y401/01071C12Y602/01004Y02P20/52
    • The invention provides a non-naturally occurring microbial biocatalyst including a microbial organism having a 4-hydroxybutanoic acid (4-HB) biosynthetic pathway having at least one exogenous nucleic acid encoding 4-hydroxybutanoate dehydrogenase, succinyl-CoA synthetase, CoA-dependent succinic semialdehyde dehydrogenase, or α-ketoglutarate decarboxylase, wherein the exogenous nucleic acid is expressed in sufficient amounts to produce monomeric 4-hydroxybutanoic acid (4-HB). Also provided is a non-naturally occurring microbial biocatalyst including a microbial organism having 4-hydroxybutanoic acid (4-HB) and 1,4-butanediol (BDO) biosynthetic pathways, the pathways include at least one exogenous nucleic acid encoding 4-hydroxybutanoate dehydrogenase, succinyl-CoA synthetase, CoA-dependent succinic semialdehyde dehydrogenase, 4-hydroxybutyrate:CoA transferase, 4-butyrate kinase, phosphotransbutyrylase, α-ketoglutarate decarboxylase, aldehyde dehydrogenase, alcohol dehydrogenase or an aldehyde/alcohol dehydrogenase, wherein the exogenous nucleic acid is expressed in sufficient amounts to produce 1,4- butanediol (BDO). Additionally provided is a method for the production of 4-HB. The method includes culturing a non-naturally occurring microbial organism having a A- hydroxybutanoic acid (4-HB) biosynthetic pathway including at least one exogenous nucleic acid encoding 4-hydroxybutanoate dehydrogenase, succinyl-CoA synthetase, CoA-dependent succinic semialdehyde dehydrogenase or α-ketoglutarate decarboxylase under substantially anaerobic conditions for a sufficient period of time to produce monomeric A- hydroxybutanoic acid (4-HB). Further provided is a method for the production of BDO. The method includes culturing a non-naturally occurring microbial biocatalyst, comprising a microbial organism having 4-hydroxybutanoic acid (4-HB) and 1,4-butanediol (BDO) biosynthetic pathways, the pathways including at least one exogenous nucleic acid encoding 4-hydroxybutanoate dehydrogenase, succinyl-CoA synthetase, CoA-dependent succinic semialdehyde dehydrogenase, 4-hydroxybutyrate:CoA transferase, 4-hydroxybutyrate kinase, phosphotranshydroxybutyrylase, α-ketoglutarate decarboxylase, aldehyde dehydrogenase, alcohol dehydrogenase or an aldehyde/alcohol dehydrogenase for a sufficient period of time to produce 1,4-butanediol (BDO). The 4-HB and/or BDO products can be secreted into the culture medium.
    • 本发明提供一种非天然存在的微生物生物催化剂,其包括具有4-羟基丁酸(4-HB)生物合成途径的微生物生物,其具有至少一种编码4-羟基丁酸脱氢酶的外源核酸,琥珀酰辅酶A合成酶,CoA依赖性琥珀酸半醛 脱氢酶或α-酮戊二酸脱羧酶,其中外源核酸以足够的量表达以产生单体4-羟基丁酸(4-HB)。 还提供了非天然存在的微生物生物催化剂,其包括具有4-羟基丁酸(4-HB)和1,4-丁二醇(BDO)生物合成途径的微生物生物,该途径包括至少一种编码4-羟基丁酸脱氢酶的外源核酸 ,琥珀酰辅酶A合成酶,CoA依赖性琥珀酸半醛脱氢酶,4-羟基丁酸酯:CoA转移酶,4-丁酸激酶,磷酸转移酶,α-酮戊二酸脱羧酶,醛脱氢酶,醇脱氢酶或醛/醇脱氢酶,其中外源核酸为 表达量足以生产1,4-丁二醇(BDO)。 另外提供了用于生产4-HB的方法。 所述方法包括培养具有4-羟基丁酸(4-HB)生物合成途径的非天然存在的微生物生物,其包含至少一种编码4-羟基丁酸脱氢酶的外源核酸,琥珀酰辅酶A合成酶,辅酶A依赖性琥珀酸半醛脱氢酶或 - 酮戊二酸脱羧酶在充分的厌氧条件下持续足够的时间以产生单体的4-羟基丁酸(4-HB)。 进一步提供一种生产BDO的方法。 该方法包括培养非天然存在的微生物生物催化剂,其包含具有4-羟基丁酸(4-HB)和1,4-丁二醇(BDO)生物合成途径的微生物生物,所述途径包括至少一种编码4- 羟基丁酸脱氢酶,琥珀酰辅酶A合成酶,CoA依赖性琥珀酸脱氢酶,4-羟基丁酸酯:CoA转移酶,4-羟基丁酸酯激酶,磷酸转移羟基丁酸酶,α-酮戊二酸脱羧酶,醛脱氢酶,醇脱氢酶或醛/醇脱氢酶, 时间来生产1,4-丁二醇(BDO)。 4-HB和/或BDO产物可以分泌到培养基中。