专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明申请

WO2014071286A1 MICROORGANISMS FOR ENHANCING THE AVAILABILITY OF REDUCING EQUIVALENTS IN THE PRESENCE OF METHANOL, AND FOR PRODUCING 1,2-PROPANEDIOL 审中-公开
标题翻译：用于提高甲醇存在下降低等效物质和生产1,2-丙二醇的可利用性的微生物
公开(公告)号：WO2014071286A1
公开(公告)日：2014-05-08
申请号：PCT/US2013/068254
申请日：2013-11-04
申请人： GENOMATICA, INC.
发明人： BURGARD, Anthony, P. , OSTERHOUT, Robin, E. , VAN DIEN, Stephen, J. , TRACEWELL, Cara, Ann , PHARKYA, Priti , ANDRE, Stefan
IPC分类号： C07C31/22 , C12P7/20
CPC分类号： C12P7/24 , C12N1/14 , C12N1/16 , C12N1/20 , C12N9/0006 , C12N9/0028 , C12N9/1007 , C12N15/52 , C12P7/04 , C12P7/18 , C12P7/20 , C12Y101/01244 , C12Y105/0102
摘要： Provided herein is a non-naturally occurring microbial organism (NNOMO) having a methanol metabolic pathway (MMP) that can enhance the availability of reducing equivalents in the presence of methanol. Such reducing equivalents can be used to increase the product yield of organic compounds produced by the microbial organism, such as 1,2-propanediol (1,2-PDO), n- propanol (n-PrOH), 1,3-propanediol (1,3-PDO) or glycerol. Also provided herein are methods for using such an organism to produce 1,2-PDO, n-PrOH, 1,3-PDO or glycerol.
摘要翻译：本文提供了具有甲醇代谢途径（MMP）的非天然存在的微生物生物体（NNOMO），其可以在甲醇存在下增强还原当量的可用性。这种还原当量可用于提高由微生物生产的有机化合物的产物产率，例如1,2-丙二醇（1,2-PDO），正丙醇（n-PrOH），1,3-丙二醇（ 1,3-PDO）或甘油。本文还提供了使用这种生物体产生1,2-PDO，n-PrOH，1,3-PDO或甘油的方法。

2. 发明申请

WO2010141920A2 MICROORGANISMS FOR THE PRODUCTION OF 1,4-BUTANEDIOL AND RELATED METHODS 审中-公开
标题翻译：用于生产1,4-丁二醇的微生物及相关方法
公开(公告)号：WO2010141920A2
公开(公告)日：2010-12-09
申请号：PCT/US2010/037544
申请日：2010-06-04
申请人： GENOMATICA, INC. , VAN DIEN, Stephen, J. , BURGARD, Anthony, P. , HASELBECK, Robert , PUJOL-BAXLEY, Catherine, J. , NIU, Wei , TRAWICK, John, D. , YIM, Harry , BURK, Mark, J. , OSTERHOUT, Robin, E. , SUN, Jun
发明人： VAN DIEN, Stephen, J. , BURGARD, Anthony, P. , HASELBECK, Robert , PUJOL-BAXLEY, Catherine, J. , NIU, Wei , TRAWICK, John, D. , YIM, Harry , BURK, Mark, J. , OSTERHOUT, Robin, E. , SUN, Jun
IPC分类号： C12P7/18
CPC分类号： C12P7/18 , C12N9/0006 , C12N9/0008 , C12N9/001 , C12N9/13 , C12N9/16 , C12N9/88 , C12N9/93 , C12N15/52
摘要： The invention provides non-naturally occurring microbial organisms comprising a 1,4-butanediol (BDO) pathway comprising at least one exogenous nucleic acid encoding a BDO pathway enzyme expressed in a sufficient amount to produce BDO and further optimized for expression of BDO. The invention additionally provides methods of using such microbial organisms to produce BDO.
摘要翻译：本发明提供包含1,4-丁二醇（BDO）途径的非天然存在的微生物生物，其包含至少一种编码足够量的BDO途径酶的外源核酸以产生BDO并进一步优化BDO的表达。本发明还提供了使用这种微生物生产BDO的方法。

3. 发明申请

WO2013184602A2 MICROORGANISMS AND METHODS FOR PRODUCTION OF 4-HYDROXYBUTYRATE, 1,4-BUTANEDIOL AND RELATED COMPOUNDS 审中-公开
标题翻译：用于生产4-羟基丁酸酯，1,4-丁二醇和相关化合物的微生物和方法
公开(公告)号：WO2013184602A2
公开(公告)日：2013-12-12
申请号：PCT/US2013/043954
申请日：2013-06-03
申请人： GENOMATICA, INC. , BURK, Mark, J.
发明人： BURK, Mark, J. , PHARKYA, Priti , BURGARD, Anthony, P. , VAN DIEN, Stephen, J. , OSTERHOUT, Robin, E. , TRAWICK, John, D. , KUCHINSKAS, Michael, P. , STEER, Brian
IPC分类号： C12P7/42 , C12N15/63
CPC分类号： C12N1/20 , C07C31/207 , C07C59/01 , C08G18/284 , C08G18/3206 , C08G63/06 , C08G63/16 , C08G63/181 , C08G69/02 , C12N9/18 , C12N15/52 , C12N15/63 , C12P7/18 , C12P7/42 , C12Y301/01 , Y02P20/52
摘要： The invention provides non-naturally occurring microbial organisms having a 4- hydroxybutyrate, 1,4-butanediol, or other product pathway and being capable of producing 4- hydroxybutyrate, 1,4-butanediol, or other product, wherein the microbial organism comprises one or more genetic modifications. The invention additionally provides methods of producing 4- hydroxybutyrate, 1,4-butanediol, or other product or related products using the microbial organisms.
摘要翻译：本发明提供具有4-羟基丁酸酯，1,4-丁二醇或其它产物途径的非天然存在的微生物生物，并能够生产4-羟基丁酸酯，1,4-丁二醇或其它产品，其中微生物包含一种或更多遗传修饰。本发明另外提供了使用微生物生物制备4-羟基丁酸酯，1,4-丁二醇或其它产品或相关产品的方法。

4. 发明申请

WO2009045637A2 METHODS FOR THE SYNTHESIS OF OLEFINS AND DERIVATIVES 审中-公开
标题翻译：用于合成烯烃和衍生物的方法
公开(公告)号：WO2009045637A2
公开(公告)日：2009-04-09
申请号：PCT/US2008/072679
申请日：2008-08-08
申请人： GENOMATICA, INC. , BURK, Mark, J. , PHARKYA, Priti , VAN DIEN, Stephen, J. , BURGARD, Anthony, P. , SCHILLING, Christopher, H.
发明人： BURK, Mark, J. , PHARKYA, Priti , VAN DIEN, Stephen, J. , BURGARD, Anthony, P. , SCHILLING, Christopher, H.
IPC分类号： C07C51/347 , C07C67/333 , C12P7/46 , C07C57/04 , C07C69/54
CPC分类号： C07C51/353 , C07C51/38 , C07C57/04 , C07C67/08 , C07C67/32 , C07C67/333 , C12P7/40 , C12P7/46 , C12P7/62 , Y02P20/125 , C07C69/60 , C07C69/54
摘要： The invention provides a method of producing acrylic acid. The method includes contacting fumaric acid with a sufficient amount of ethylene in the presence of a cross-metathesis transformation catalyst to produce about two moles of acrylic acid per mole of fumaric acid. Also provided is an acrylate ester. The method includes contacting fumarate diester with a sufficient amount of ethylene in the presence of a cross-metathesis transformation catalyst to produce about two moles of acrylate ester per mole of fumarate diester. An integrated process for process for producing acrylic acid or acrylate ester is provided which couples bioproduction of fumaric acid with metathesis transformation. An acrylic acid and an acrylate ester production also is provided.
摘要翻译：本发明提供了一种生产丙烯酸的方法。该方法包括使富马酸与足够量的乙烯在交叉复分解转化催化剂存在下接触以每摩尔富马酸产生约2摩尔丙烯酸。还提供了丙烯酸酯。该方法包括使富马酸二酯与足够量的乙烯在交叉复分解转化催化剂存在下接触，以每摩尔富马酸二酯产生约2摩尔丙烯酸酯。提供了一种生产丙烯酸或丙烯酸酯的方法的综合方法，其将富马酸的生物生产与复分解转化相结合。还提供丙烯酸和丙烯酸酯生产。

5. 发明申请

WO2015100338A2 METHODS AND ORGANISMS WITH INCREASED CARBON FLUX EFFICIENCIES 审中-公开
标题翻译：具有增加碳通量效率的方法和有机体
公开(公告)号：WO2015100338A2
公开(公告)日：2015-07-02
申请号：PCT/US2014/072178
申请日：2014-12-23
申请人： GENOMATICA, INC.
发明人： BURGARD, Anthony, P. , OSTERHOUT, Robin, E. , VAN DIEN, Stephen, J. , PHARKYA, Priti , YANG, Tae, Hoon , CHOI, Jungik
IPC分类号： C12N1/21
CPC分类号： C12P7/18 , C07K14/245 , C12N1/20 , C12N9/0036 , C12N9/0057 , C12N9/1205 , C12N9/16 , C12N9/52 , C12N9/88 , C12N9/93 , C12N15/52 , C12P7/42 , C12Y106/01002 , C12Y110/03 , C12Y207/0104 , C12Y301/00 , C12Y301/02 , C12Y301/02002 , C12Y304/21092 , C12Y401/01031 , C12Y401/01032
摘要： The invention is directed to a non-naturally occurring microbial organism comprising a first attenuation of a succinyl-CoA synthetase or transferase and at least a second attenuation of a succinyl-CoA converting enzyme or a gene encoding a succinate producing enzyme within a multi-step pathway having a net conversion of succinyl-CoA to succinate.
摘要翻译：本发明涉及一种非天然存在的微生物生物体，其包含琥珀酰-CoA合成酶或转移酶的第一次衰减，以及琥珀酰-CoA转化酶或编码多步骤中产琥珀酸酶的基因的至少第二次衰减途径具有琥珀酰-CoA向琥珀酸盐的净转化。

6. 发明申请

WO2014066235A1 MICROORGANISMS AND METHODS FOR ENHANCING THE AVAILABILITY OF REDUCING EQUIVALENTS IN THE PRESENCE OF METHANOL, AND FOR PRODUCING SUCCINATE RELATED THERETO 审中-公开
标题翻译：用于提高甲醇存在下减少等效物的可行性的微生物和方法以及用于生产与其相关的成分
公开(公告)号：WO2014066235A1
公开(公告)日：2014-05-01
申请号：PCT/US2013/065887
申请日：2013-10-21
申请人： GENOMATICA, INC.
发明人： BURGARD, Anthony, P. , OSTERHOUT, Robin, E. , VAN DIEN, Stephen, J. , TRACEWELL, Cara, Ann , PHARKYA, Priti , ANDRAE, Stefan
IPC分类号： C12P1/04
CPC分类号： C12P7/46 , C07C55/10 , C07C59/235 , C08G63/00 , C12N9/0006 , C12N9/0028 , C12N9/1007 , C12N15/70 , C12N15/74 , C12N15/77 , C12N15/81 , C12N15/815 , C12P7/24 , C12Y101/01244 , C12Y105/0102
摘要： Provided herein is a non-naturally occurring microbial organism (NNOMO) having a methanol metabolic pathway (MMP) that can enhance the availability of reducing equivalents in the presence of methanol. Such reducing equivalents can be used to increase the product yield of organic compounds produced by the microbial organism, such as succinate. Also provided herein are methods for using such an organism to produce succinate.
摘要翻译：本文提供了具有甲醇代谢途径（MMP）的非天然存在的微生物生物体（NNOMO），其可以在甲醇存在下增强还原当量的可用性。这种还原当量可以用于增加由微生物生物如琥珀酸酯产生的有机化合物的产物产率。本文还提供了使用这种生物体来生产琥珀酸盐的方法。

7. 发明申请

WO2014035925A1 MICROORGANISMS AND METHODS FOR ENHANCING THE AVAILABILITY OF REDUCING EQUIVALENTS IN THE PRESENCE OF METHANOL, AND FOR PRODUCING 1,4-BUTANEDIOL RELATED THERETO 审中-公开
标题翻译：用于提高甲醇存在下降低等效物质和生产1,4-丁二醇相关物质的可行性的微生物和方法
公开(公告)号：WO2014035925A1
公开(公告)日：2014-03-06
申请号：PCT/US2013/056725
申请日：2013-08-27
申请人： GENOMATICA, INC.
发明人： BURGARD, Anthony, P. , OSTERHOUT, Robin, E. , VAN DIEN, Stephen, J. , TRACEWELL, Cara, Ann , PHARKYA, Priti , ANDRAE, Stefan
IPC分类号： C12P7/18 , C07C31/18 , C12N1/21
CPC分类号： C12P7/18 , C07D207/12 , C08G18/3206 , C08G63/06 , C08G63/12 , C08G63/66 , C08G69/26 , C12N9/0006 , C12N9/0028 , C12N9/1007 , C12N9/88 , C12P7/24 , C12P19/02 , C12Y101/01224 , C12Y101/01244 , C12Y101/02007 , C12Y105/0102 , C12Y201/01 , C12Y201/0109 , C12Y402/01 , Y02P20/52
摘要： Provided herein is a non-naturally occurring microbial organism having a methanol metabolic pathway (MMP) that can enhance the availability of reducing equivalents in the presence of methanol. Such reducing equivalents can be used to increase the product yield of organic compounds produced by the microbial organism, such as 1,4-butanediol (BDO). Also provided herein are methods for using such an organism to produce BDO.
摘要翻译：本文提供了具有甲醇代谢途径（MMP）的非天然存在的微生物生物，其可以在甲醇存在下增强还原当量的可用性。这种还原当量可用于增加由微生物生物例如1,4-丁二醇（BDO）产生的有机化合物的产物产率。本文还提供了使用这种生物体产生BDO的方法。

8. 发明申请

WO2009023493A1 METHODS AND ORGANISMS FOR THE GROWTH-COUPLED PRODUCTION OF 1,4-BUTANEDIOL 审中-公开
标题翻译：用于1,4-丁二醇的生长联合生产的方法和有机体
公开(公告)号：WO2009023493A1
公开(公告)日：2009-02-19
申请号：PCT/US2008/072362
申请日：2008-08-06
申请人： GENOMATICA, INC. , BURGARD, Anthony, P. , VAN DIEN, Stephen, J. , BURK, Mark, J.
发明人： BURGARD, Anthony, P. , VAN DIEN, Stephen, J. , BURK, Mark, J.
IPC分类号： C12N15/52 , C12P7/18 , C12N1/00
CPC分类号： C12P7/18 , B01D3/002 , C12N9/0006
摘要： The invention provides a non- naturally occurring microorganism comprising one or more gene disruptions, the one or more gene disruptions occurring in genes encoding an enzyme obligatory to coupling 1,4-butanediol production to growth of the microorganism when the gene disruption reduces an activity of the enzyme, whereby theone or more gene disruptions confers stable growth-coupled production of 1,4-butanediol onto the non- naturally occurring microorganism. The microorganism can further comprise a gene encoding an enzyme in a 1,4- butanediol (BDO) biosynthetic pathway. The invention additionally relates to methods of using microorganisms to produce BDO.
摘要翻译：本发明提供了一种非天然存在的微生物，其包含一种或多种基因破坏，所述一个或多个基因破坏发生在编码需要将1,4-丁二醇产生与微生物生长偶联的酶的基因中，当基因破坏降低活性时所述酶，其中所述一种或多种基因破坏赋予所述非天然存在的微生物上1,4-丁二醇稳定的生长偶联产生。微生物还可以包含编码1,4-丁二醇（BDO）生物合成途径中的酶的基因。本发明还涉及使用微生物来生产BDO的方法。

9. 发明申请

WO2014099725A1 MICROORGANISMS AND METHODS FOR ENHANCING THE AVAILABILITY OF REDUCING EQUIVALENTS IN THE PRESENCE OF METHANOL, AND FOR PRODUCING ADIPATE, 6-AMINOCAPROATE, HEXAMETHYLENEDIAMINE OR CAPROLACTAM RELATED THERETO 审中-公开
标题翻译：用于提高甲醇存在下降低等效物的可行性的微生物和方法，以及用于生产与其相关的丙氨酸，6-氨基己酸，十六烷二胺或其衍生物的微生物和方法
公开(公告)号：WO2014099725A1
公开(公告)日：2014-06-26
申请号：PCT/US2013/075287
申请日：2013-12-16
申请人： GENOMATICA, INC.
发明人： BURGARD, Anthony, P. , OSTERHOUT, Robin, E. , VAN DIEN, Stephen, J. , TRACEWELL, Cara, Ann , PHARKYA, Priti , ANDRAE, Stefan
IPC分类号： C12N1/21
CPC分类号： C12P17/10 , A23L29/065 , C08G63/78 , C08G69/08 , C08G69/16 , C08G73/0213 , C12N9/0006 , C12N15/52 , C12P7/24 , C12P7/44 , C12P13/001 , C12P13/005 , C12Y101/01244
摘要： Provided herein is a non-naturally occurring microbial organism having a methanol metabolic pathway that can enhance the availability of reducing equivalents in the presence of methanol. Such reducing equivalents can be used to increase the product yield of organic compounds produced by the microbial organism, such as adipate, 6-aminocaproate, hexamethylenediamine or caprolactam. Also provided herein are methods for using such an organism to produce adipate, 6-aminocaproate, hexamethylenediamine or caprolactam.
摘要翻译：本文提供了具有甲醇代谢途径的非天然存在的微生物生物，其可以在甲醇存在下增强还原当量的可用性。这样的还原当量可用于增加微生物生物体如己二酸，6-氨基己酸，六亚甲基二胺或己内酰胺产生的有机化合物的产物产率。本文还提供了使用这种生物体产生己二酸，6-氨基己酸，六亚甲基二胺或己内酰胺的方法。

10. 发明申请

WO2008115840A2 COMPOSITIONS AND METHODS FOR THE BIOSYNTHESIS OF 1,4-BUTANEDIOL AND ITS PRECURSORS 审中-公开
标题翻译： 1,4-丁二醇及其前体的生物合成的组合物和方法
公开(公告)号：WO2008115840A2
公开(公告)日：2008-09-25
申请号：PCT/US2008/057168
申请日：2008-03-14
申请人： GENOMATICA, INC. , BURK, Mark, J. , VAN DIEN, Stephen, J. , BURGARD, Anthony , NIU, Wei
发明人： BURK, Mark, J. , VAN DIEN, Stephen, J. , BURGARD, Anthony , NIU, Wei
IPC分类号： C12N1/21
CPC分类号： C12N15/52 , B01D3/002 , C12N9/0006 , C12N9/0008 , C12N9/88 , C12N9/93 , C12N15/70 , C12N15/81 , C12P7/18 , C12P7/42 , C12P7/52 , C12P17/04 , C12Y101/01061 , C12Y102/01076 , C12Y401/01071 , C12Y602/01004 , Y02P20/52
摘要： The invention provides a non-naturally occurring microbial biocatalyst including a microbial organism having a 4-hydroxybutanoic acid (4-HB) biosynthetic pathway having at least one exogenous nucleic acid encoding 4-hydroxybutanoate dehydrogenase, succinyl-CoA synthetase, CoA-dependent succinic semialdehyde dehydrogenase, or α-ketoglutarate decarboxylase, wherein the exogenous nucleic acid is expressed in sufficient amounts to produce monomeric 4-hydroxybutanoic acid (4-HB). Also provided is a non-naturally occurring microbial biocatalyst including a microbial organism having 4-hydroxybutanoic acid (4-HB) and 1,4-butanediol (BDO) biosynthetic pathways, the pathways include at least one exogenous nucleic acid encoding 4-hydroxybutanoate dehydrogenase, succinyl-CoA synthetase, CoA-dependent succinic semialdehyde dehydrogenase, 4-hydroxybutyrate:CoA transferase, 4-butyrate kinase, phosphotransbutyrylase, α-ketoglutarate decarboxylase, aldehyde dehydrogenase, alcohol dehydrogenase or an aldehyde/alcohol dehydrogenase, wherein the exogenous nucleic acid is expressed in sufficient amounts to produce 1,4- butanediol (BDO). Additionally provided is a method for the production of 4-HB. The method includes culturing a non-naturally occurring microbial organism having a A- hydroxybutanoic acid (4-HB) biosynthetic pathway including at least one exogenous nucleic acid encoding 4-hydroxybutanoate dehydrogenase, succinyl-CoA synthetase, CoA-dependent succinic semialdehyde dehydrogenase or α-ketoglutarate decarboxylase under substantially anaerobic conditions for a sufficient period of time to produce monomeric A- hydroxybutanoic acid (4-HB). Further provided is a method for the production of BDO. The method includes culturing a non-naturally occurring microbial biocatalyst, comprising a microbial organism having 4-hydroxybutanoic acid (4-HB) and 1,4-butanediol (BDO) biosynthetic pathways, the pathways including at least one exogenous nucleic acid encoding 4-hydroxybutanoate dehydrogenase, succinyl-CoA synthetase, CoA-dependent succinic semialdehyde dehydrogenase, 4-hydroxybutyrate:CoA transferase, 4-hydroxybutyrate kinase, phosphotranshydroxybutyrylase, α-ketoglutarate decarboxylase, aldehyde dehydrogenase, alcohol dehydrogenase or an aldehyde/alcohol dehydrogenase for a sufficient period of time to produce 1,4-butanediol (BDO). The 4-HB and/or BDO products can be secreted into the culture medium.
摘要翻译：本发明提供一种非天然存在的微生物生物催化剂，其包括具有4-羟基丁酸（4-HB）生物合成途径的微生物生物，其具有至少一种编码4-羟基丁酸脱氢酶的外源核酸，琥珀酰辅酶A合成酶，CoA依赖性琥珀酸半醛脱氢酶或α-酮戊二酸脱羧酶，其中外源核酸以足够的量表达以产生单体4-羟基丁酸（4-HB）。还提供了非天然存在的微生物生物催化剂，其包括具有4-羟基丁酸（4-HB）和1,4-丁二醇（BDO）生物合成途径的微生物生物，该途径包括至少一种编码4-羟基丁酸脱氢酶的外源核酸，琥珀酰辅酶A合成酶，CoA依赖性琥珀酸半醛脱氢酶，4-羟基丁酸酯：CoA转移酶，4-丁酸激酶，磷酸转移酶，α-酮戊二酸脱羧酶，醛脱氢酶，醇脱氢酶或醛/醇脱氢酶，其中外源核酸为表达量足以生产1,4-丁二醇（BDO）。另外提供了用于生产4-HB的方法。所述方法包括培养具有4-羟基丁酸（4-HB）生物合成途径的非天然存在的微生物生物，其包含至少一种编码4-羟基丁酸脱氢酶的外源核酸，琥珀酰辅酶A合成酶，辅酶A依赖性琥珀酸半醛脱氢酶或 - 酮戊二酸脱羧酶在充分的厌氧条件下持续足够的时间以产生单体的4-羟基丁酸（4-HB）。进一步提供一种生产BDO的方法。该方法包括培养非天然存在的微生物生物催化剂，其包含具有4-羟基丁酸（4-HB）和1,4-丁二醇（BDO）生物合成途径的微生物生物，所述途径包括至少一种编码4- 羟基丁酸脱氢酶，琥珀酰辅酶A合成酶，CoA依赖性琥珀酸脱氢酶，4-羟基丁酸酯：CoA转移酶，4-羟基丁酸酯激酶，磷酸转移羟基丁酸酶，α-酮戊二酸脱羧酶，醛脱氢酶，醇脱氢酶或醛/醇脱氢酶，时间来生产1,4-丁二醇（BDO）。 4-HB和/或BDO产物可以分泌到培养基中。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式