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1. 发明申请

WO2011143478A2 IDENTIFICATION OF NUCLEIC ACIDS 审中-公开
标题翻译：核酸鉴定
公开(公告)号：WO2011143478A2
公开(公告)日：2011-11-17
申请号：PCT/US2011/036320
申请日：2011-05-12
申请人： LIFE TECHNOLOGIES CORPORATION , SERGEEV, Nikolay V. , BREVNOV, Maxim G. , FURTADO, Manohar R.
发明人： SERGEEV, Nikolay V. , BREVNOV, Maxim G. , FURTADO, Manohar R.
IPC分类号： C12Q1/68 , C12N15/11 , G01N33/58
CPC分类号： C12Q1/6853 , C12Q1/6818 , C12Q1/686 , Y02A50/52 , C12Q2565/1025 , C12Q2563/173 , C12Q2527/107 , C12Q2561/101
摘要： This disclosure relates to methods for identifying target nucleic acids in a sample by detecting an amplified sequence corresponding to the target using a detectable probe and by monitoring its melting temperature (T m ).
摘要翻译：本公开涉及通过使用可检测的探针检测对应于靶的扩增序列并通过监测其解链温度（Tm）来鉴定样品中的靶核酸的方法。

2. 发明申请

WO2010002883A2 METHODS AND DEVICES FOR SINGLE-MOLECULE WHOLE GENOME ANALYSIS 审中-公开
标题翻译：用于单分子全基因组分析的方法和设备
公开(公告)号：WO2010002883A2
公开(公告)日：2010-01-07
申请号：PCT/US2009049244
申请日：2009-06-30
申请人： BIONANOMATRIX INC , XIAO MING , DESHPANDE PARIKSHIT A , CAO HAN , AUSTIN MICHAEL , VIJAYAN KANDASWAMY , SHARONOV ALEXEY Y , BOYCE-JACINO MICHAEL
发明人： XIAO MING , DESHPANDE PARIKSHIT A , CAO HAN , AUSTIN MICHAEL , VIJAYAN KANDASWAMY , SHARONOV ALEXEY Y , BOYCE-JACINO MICHAEL
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6818 , C12Q1/6806 , C12Q1/6825 , C12Q1/6869 , G06F19/18 , C12Q2565/629 , C12Q2565/1015 , C12Q2521/301 , C12Q2537/1376 , C12Q2561/109 , C12Q2565/1025 , C12Q2565/631
摘要： Provided are methods and devices for single-molecule genomic analysis. In one embodiment, the methods entail processing a double-stranded nucleic acid and characterizing said nucleic acid. These methods are useful in, e.g., determining structural variations and copy number variations between individuals.
摘要翻译：提供单分子基因组分析的方法和装置。在一个实施方案中，所述方法需要处理双链核酸并表征所述核酸。这些方法在例如确定个体之间的结构变化和拷贝数变化方面是有用的。

3. 发明申请

WO2005051967A2 OLIGONUCLEOTIDES LABELED WITH A PLURALITY OF FLUOROPHORES 审中-公开
标题翻译：标有多种荧光素的寡核苷酸
公开(公告)号：WO2005051967A2
公开(公告)日：2005-06-09
申请号：PCT/US2004038946
申请日：2004-11-19
申请人： ALLELOGIC BIOSCIENCES CORP , MAO FEI , XIN XING
发明人： MAO FEI , XIN XING
IPC分类号： C07H20060101 , C07H21/04 , C12Q1/68 , C07H
CPC分类号： C12Q1/6818 , C12Q1/6851 , Y10S435/975 , C12Q2565/1015 , C12Q2561/101 , C12Q2565/1025
摘要： An embodiment of the invention discloses new methods for designing labeled nucleic acid probes and primers by labeling oligonucleotides with a plurality of spectrally identical or similar dyes and optionally with one or more quencher dyes. Oligonucleotides labeled in accordance with some embodiments of the invention exhibit a detectable increase in signal, for example, fluorescent signal when the labeling dyes are separated from one another. Methods for separating the dye include cleaving the labeled oligonucleotides include using enzymes that have 5'-exonuclease activity. In one embodiment nucleic acid primers of the present invention may fluoresce upon hybridization to a target sequence and incorporation into the amplification product. Nucleic acid probes and primers of the present invention have wide applications ranging from general detection of a target nucleic acid sequence to clinical diagnostics. Major advantages of the oligonucleotides including nucleic acid probes and primers of many embodiments of the present invention are their synthetic simplicity, spectral versatility and superior fluorescent signal.
摘要翻译：本发明的一个实施方案公开了通过用多个光谱相同或相似的染料和任选地与一种或多种猝灭剂染料标记寡核苷酸来设计标记的核酸探针和引物的新方法。根据本发明的一些实施方案标记的寡核苷酸在标记染料彼此分离时表现出可检测的信号增加，例如荧光信号。用于分离染料的方法包括切割标记的寡核苷酸包括使用具有5'-外切核酸酶活性的酶。在一个实施方案中，本发明的核酸引物可以在与靶序列杂交时发荧光，并掺入扩增产物。本发明的核酸探针和引物具有广泛的应用范围，从靶核酸序列的一般检测到临床诊断。包括本发明许多实施方案的核酸探针和引物的寡核苷酸的主要优点是它们的合成简单性，光谱通用性和优异的荧光信号。

4. 发明申请

WO2005030996A2 PROGRAMMABLE MOLECULAR BARCODES 审中-公开
标题翻译：可编程分子杆
公开(公告)号：WO2005030996A2
公开(公告)日：2005-04-07
申请号：PCT/US2004031289
申请日：2004-09-23
申请人： INTEL CORP
发明人： SU XING , KOO TAE-WOONG , BERLIN ANDREW , SUN LEI , SUNDARARAJAN NARAYANAN , YAMAKAWA MINEO
IPC分类号： C12Q1/68 , G06K19/06
CPC分类号： B82Y10/00 , B82Y5/00 , C12Q1/6816 , G06K19/06028 , C12Q2525/161 , C12Q2537/143 , C12Q2565/1025
摘要： The present disclosure concerns methods for producing and/or using molecular barcodes. In certain embodiments of the invention, the barcodes comprise polymer backbones that may contain one or more branch structures. Tags may be attached to the backbone and/or branch structures. The barcode may also comprise a probe that can bind to a target, such as proteins, nucleic acids and other biomolecules or aggregates. Different barcodes may be distinguished by the type and location of the tags. In other embodiments, barcodes may be produced by hybridization of one or more tagged oligonucleotides to a template, comprising a container section and a probe section. The tagged oligonucleotides may be designed as modular code sections, to form different barcodes specific for different targets. In alternative embodiments, barcodes may be prepared by polymerization of monomeric units. Bound barcodes may be detected by various imaging modalities, such as, surface plasmon resonance, fluorescent or Raman spectroscopy.
摘要翻译：本公开涉及用于生产和/或使用分子条形码的方法。在本发明的某些实施方案中，条形码包含可包含一个或多个分支结构的聚合物主链。标签可以附加到骨干和/或分支结构。条形码还可以包含可以与靶标结合的探针，例如蛋白质，核酸和其他生物分子或聚集体。可以通过标签的类型和位置区分不同的条形码。在其它实施方案中，条形码可以通过将一个或多个标记的寡核苷酸与包含容器部分和探针部分的模板杂交来产生。标记的寡核苷酸可以被设计为模块代码部分，以形成针对不同靶标的不同条形码。在替代实施例中，条形码可以通过单体单元的聚合来制备。可以通过各种成像方式，例如表面等离子体共振，荧光或拉曼光谱来检测结合条形码。

5. 发明申请

WO00005357A1 LABELED COMPLEX, PROCESS FOR PRODUCING THE SAME AND PROCESS FOR UTILIZING THE SAME 审中-公开
标题翻译：标记复合物，其制备方法和使用该方法的方法
公开(公告)号：WO00005357A1
公开(公告)日：2000-02-03
申请号：PCT/JP1999/003824
申请日：1999-07-15
IPC分类号： C07K1/04 , C12Q1/68 , C12N15/10 , C07K1/22 , C12N11/00
CPC分类号： C07K1/047 , C12Q1/6816 , C12Q2537/143 , C12Q2565/1025 , C12Q2563/149 , C12Q2563/143
摘要： A labeled complex which makes it possible, as a multimolecular marking in combinatorial chemistry, to stably and clearly distinguish various fine substances of several thousand or several ten thousand order or more at a high sensitivity and a high accuracy and to simultaneously satisfy the requirements for improving the ability to capture targets, enhancing the distinguishing sensitivity and elevating the number of substances to be distinguished. The above labeled substance is composed of a fine particle, a number of target-carrying substances each bonding at one end to the fine particle, and a label bonding to each target-carrying substance at another end, wherein each of the target-carrying substances carries one or more targets or is capable of carrying the same, and the whole labeled substance is constituted so that definite targets are contained therein at a definite ratio and distributed to the support and all of the target-carrying substances bonded thereto.
摘要翻译：标记的复合物，可以作为组合化学中的多分子标记，以高灵敏度和高精度稳定和清晰地分辨数千或数万级以上的各种细小物质，同时满足改善的要求捕获目标的能力，增强区分灵敏度和提高待区分的物质数量。上述标记物质由微粒，一端与微粒接合的目标载体物质数量，另一端与各载带物质结合的标记物构成，其中，各载带物质携带一个或多个靶标或能够携带它们，并且构成整个标记物质，以确定的比例含有确定的靶标并分配给载体和与其结合的所有目标载体物质。

6. 发明申请

WO1996040902A1 NUCLEIC ACID REPAIR ENZYME METHODS FOR POINT MUTATION DETECTION AND IN VITRO MUTAGENESIS 审中-公开
标题翻译：用于点突变检测和体外诱变的核酸修复酶
公开(公告)号：WO1996040902A1
公开(公告)日：1996-12-19
申请号：PCT/US1996008694
申请日：1996-06-07
申请人： TREVIGEN, INC.
发明人： TREVIGEN, INC. , CHIRIKJIAN, Jack, G. , COLLIER, Bruce, G.
IPC分类号： C12N15/10
CPC分类号： C12Q1/6823 , C12N15/102 , C12Q1/6818 , C12Q1/682 , C12Q1/6827 , C12Q1/6858 , C12Q2565/1025 , C12Q2537/113 , C12Q2521/307 , C12Q2535/131
摘要： The present invention provides several methods employing nucleic acid repair enzymes. The present invention provides a method for detecting point mutations in nucleic acid sequences. The present invention further provides a method for detecting non-mutated or wild-type nucleic acid sequences. The present invention also enhances target polynucleotide detection using an oscillation reaction and tail labeling techniques, as well as by linking the nucleic acid repair enzyme to a probe molecule. The present invention also provides helix destabilizing molecule and similar molecules to enhance the hybridization of the probe to the target polynucleotides. This invention further provides for the use of thermally stable nucleic acid repair enzymes which will facilitate reactions at elevated temperatures. This invention also provides a method for determining the repair index for a mismatched or damaged oligonucleotide probe. Finally, this invention renders a method for effecting in vitro mutagenesis of a target polynucleotide.
摘要翻译：本发明提供使用核酸修复酶的几种方法。本发明提供了一种用于检测核酸序列中的点突变的方法。本发明还提供了用于检测非突变或野生型核酸序列的方法。本发明还使用振荡反应和尾标记技术以及通过将核酸修复酶与探针分子连接来增强靶多核苷酸检测。本发明还提供螺旋不稳定分子和类似分子，以增强探针与目标多核苷酸的杂交。本发明进一步提供热稳定性核酸修复酶的使用，其将促进在升高的温度下的反应。本发明还提供了用于确定错配或受损的寡核苷酸探针的修复指数的方法。最后，本发明提供了一种用于实现靶多核苷酸的体外诱变的方法。

7. 发明申请

WO1994017397A1 DNA COMPLEXES WITH DYES DESIGNED FOR ENERGY TRANSFER AS FLUORESCENT MARKERS 审中-公开
标题翻译： DNA复合物与设计用于作为荧光标记的能量转移的染料
公开(公告)号：WO1994017397A1
公开(公告)日：1994-08-04
申请号：PCT/US1994000234
申请日：1994-01-07
申请人： THE REGENTS OF THE UNIVERSITY OF CALIFORNIA
发明人： THE REGENTS OF THE UNIVERSITY OF CALIFORNIA , GLAZER, Alexander, N. , BENSON, Scott, C.
IPC分类号： G01N27/26
CPC分类号： C07D417/14 , C07D221/12 , C07D405/12 , C07D417/06 , C09B23/04 , C12Q1/6816 , C12Q1/6818 , G01N27/44726 , G01N27/44747 , C12Q2565/1025 , C12Q2565/102
摘要： Heteromultimeric fluorophores are provided for binding to DNA, which allow for the detection of DNA in electrical separations and preparation of probes having high-fluorescent efficiencies and large Stokes shifts. In addition, by appropriate choice of fluorescent molecules, one can use a single narrow wavelength band excitation light source, while obtaining fluorescent emissions having sufficient separation to be readily discriminated.
摘要翻译：提供了异源多聚体荧光团用于结合DNA，其允许在电分离中检测DNA并制备具有高荧光效率和大的斯托克斯位移的探针。此外，通过适当选择荧光分子，可以使用单个窄波长带激发光源，同时获得具有足够分离的荧光发射以易于鉴别。

8. 发明申请

WO1994002640A1 MULTI REPORTER-LABELED NUCLEIC ACID PROBES 审中-公开
标题翻译：多个报告标签核酸探针
公开(公告)号：WO1994002640A1
公开(公告)日：1994-02-03
申请号：PCT/US1993006682
申请日：1993-07-16
申请人： APROGENEX, INC.
发明人： APROGENEX, INC. , COLVIN, David , JU, Shyh, Chen , CUBBAGE, Michael, Lee , WEBER, William, Dugald , ASGARI, Morteza , PRASHAD, Nagindra , BRESSER, Joel , BLICK, Mark
IPC分类号： C12Q01/68
CPC分类号： C12Q1/6876 , C12Q1/6816 , C12Q2565/1025 , C12Q2525/125
摘要： Nucleic acid probes, processes for using them, and processes for making them. The probes have reporter moieties such as fluorescent dyes covalently linked to phosphate atoms on the backbone of a single-stranded nucleic acid moiety.
摘要翻译：核酸探针，使用它们的方法和制备它们的方法。探针具有报道部分，例如与单链核酸部分的主链上的磷酸根原子共价连接的荧光染料。

9. 发明申请

WO2017050934A1 IMPROVED DETECTION OF SHORT HOMOPOLYMERIC REPEATS 审中-公开
标题翻译：改进短链多糖重复的检测
公开(公告)号：WO2017050934A1
公开(公告)日：2017-03-30
申请号：PCT/EP2016/072605
申请日：2016-09-22
申请人： BIOCARTIS NV
发明人： CLAES, Bart , ROSSAU, Rudi
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6858 , C12Q1/6886 , C12Q2600/158 , C12Q2527/107 , C12Q2537/143 , C12Q2565/1025 , C12Q2565/107
摘要： The present application relates to detection of changes in the number of nucleotides in short homopolymeric nucleic acid repeats, in particular in short homopolymeric microsatellites, for example for the purpose of diagnosing microsatellite instability (MSI) and/or mismatch repair (MMR-) deficiency in tumors. Accordingly, methods are provided for detecting changes in the number of nucleotides present in short homopolymeric nucleotide repeat sequences as well as kits and cartridges for automated detection of said changes.
摘要翻译：本申请涉及检测短均聚核酸重复序列（特别是短均聚微卫星）中核苷酸数量的变化，例如用于诊断微卫星不稳定性（MSI）和/或错配修复（MMR-）缺陷的目的肿瘤。因此，提供了用于检测短均聚核苷酸重复序列中存在的核苷酸数目的变化的方法以及用于自动检测所述变化的试剂盒和滤芯。

10. 发明申请

WO2014123822A1 METHODS FOR SINGLE-MOLECULE ANALYSIS 审中-公开
标题翻译：单分子分析方法
公开(公告)号：WO2014123822A1
公开(公告)日：2014-08-14
申请号：PCT/US2014/014501
申请日：2014-02-03
申请人： BIONANO GENOMICS, INC.
发明人： CAO, Han , XIAO, Ming , HASTIE, Alex, R. , SAGHBINI, Michael, G. , SADOWSKI, Henry, B.
IPC分类号： C12Q1/68 , C07H21/00
CPC分类号： C12Q1/6869 , C12Q1/6806 , C12Q1/6809 , C12Q2521/301 , C12Q2565/1025
摘要： Methods for single-molecule preparation and analysis are disclosed herein. The methods can, for example, be used for isolating and analyzing DNA from various biological samples.
摘要翻译：本文公开了单分子制备和分析的方法。该方法可以例如用于分离和分析来自各种生物样品的DNA。

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