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1. 发明申请

WO1996040902A1 NUCLEIC ACID REPAIR ENZYME METHODS FOR POINT MUTATION DETECTION AND IN VITRO MUTAGENESIS 审中-公开
标题翻译：用于点突变检测和体外诱变的核酸修复酶
公开(公告)号：WO1996040902A1
公开(公告)日：1996-12-19
申请号：PCT/US1996008694
申请日：1996-06-07
申请人： TREVIGEN, INC.
发明人： TREVIGEN, INC. , CHIRIKJIAN, Jack, G. , COLLIER, Bruce, G.
IPC分类号： C12N15/10
CPC分类号： C12Q1/6823 , C12N15/102 , C12Q1/6818 , C12Q1/682 , C12Q1/6827 , C12Q1/6858 , C12Q2565/1025 , C12Q2537/113 , C12Q2521/307 , C12Q2535/131
摘要： The present invention provides several methods employing nucleic acid repair enzymes. The present invention provides a method for detecting point mutations in nucleic acid sequences. The present invention further provides a method for detecting non-mutated or wild-type nucleic acid sequences. The present invention also enhances target polynucleotide detection using an oscillation reaction and tail labeling techniques, as well as by linking the nucleic acid repair enzyme to a probe molecule. The present invention also provides helix destabilizing molecule and similar molecules to enhance the hybridization of the probe to the target polynucleotides. This invention further provides for the use of thermally stable nucleic acid repair enzymes which will facilitate reactions at elevated temperatures. This invention also provides a method for determining the repair index for a mismatched or damaged oligonucleotide probe. Finally, this invention renders a method for effecting in vitro mutagenesis of a target polynucleotide.
摘要翻译：本发明提供使用核酸修复酶的几种方法。本发明提供了一种用于检测核酸序列中的点突变的方法。本发明还提供了用于检测非突变或野生型核酸序列的方法。本发明还使用振荡反应和尾标记技术以及通过将核酸修复酶与探针分子连接来增强靶多核苷酸检测。本发明还提供螺旋不稳定分子和类似分子，以增强探针与目标多核苷酸的杂交。本发明进一步提供热稳定性核酸修复酶的使用，其将促进在升高的温度下的反应。本发明还提供了用于确定错配或受损的寡核苷酸探针的修复指数的方法。最后，本发明提供了一种用于实现靶多核苷酸的体外诱变的方法。

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