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1. 发明申请

WO2016118085A1 SINGLE CELL RNA AND MUTATIONAL ANALYSIS PCR (SCRM-PCR): A METHOD FOR SIMULTANEOUS ANALYSIS OF DNA AND RNA AT THE SINGLE-CELL LEVEL 审中-公开
标题翻译：单细胞RNA和突变分析PCR（SCRM-PCR）：在单细胞水平上同时分析DNA和RNA的方法
公开(公告)号：WO2016118085A1
公开(公告)日：2016-07-28
申请号：PCT/SG2016/050026
申请日：2016-01-21
申请人： AGENCY FOR SCIENCE, TECHNOLOGY AND RESEARCH
发明人： TAN, Min-Han , CIMA, Igor
IPC分类号： C12Q1/68
CPC分类号： C12Q1/686 , C12Q2537/143 , C12Q2539/103 , C12Q2539/105 , C12Q2549/119
摘要： A method of simultaneously analyzing RNA and DNA in a sample, the method comprising the step (a) contacting the sample with a reverse primer from a first primer pair directed to a target RNA region to effect reverse transcription of RNA into cDNA with a reverse transcriptase; (b) subsequently contacting the sample with (i) a forward primer from the first primer pair directed to a second cDNA region, (ii) a forward and a reverse primers from a second primer pair targeted to a DNA region, and (ii) a DNA polymerase to simultaneously amplify the target cDNA and target DNA region; and (c) analyzing the amplified target cDNA region and/or amplified target DNA region. Also encompassed are uses of the method to analyze gene expression and mutations, kits comprising primers, enzymes, buffers.
摘要翻译：一种同时分析样品中的RNA和DNA的方法，所述方法包括步骤（a）使样品与来自指向靶RNA区域的第一引物对的反向引物接触，以通过逆转录酶将RNA逆转录成cDNA ; （b）随后使样品与（i）来自引导到第二cDNA区的第一引物对的正向引物接触，（ii）来自靶向DNA区的第二引物对的正向和反向引物，和（ii）同时扩增靶cDNA和靶DNA区域的DNA聚合酶; 和（c）分析扩增的靶cDNA区域和/或扩增的靶DNA区域。还包括分析基因表达和突变的方法的用途，包括引物，酶，缓冲液的试剂盒。

2. 发明申请

WO2010127186A1 NUCLEIC ACID CONSTRUCTS AND METHODS OF USE 审中-公开
标题翻译：核酸结构及其使用方法
公开(公告)号：WO2010127186A1
公开(公告)日：2010-11-04
申请号：PCT/US2010/033064
申请日：2010-04-29
申请人： PROGNOSYS BIOSCIENCES, INC. , CHEE, Mark, S.
发明人： CHEE, Mark, S.
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6869 , C12Q1/6806 , C12Q1/6809 , C12Q1/6827 , C12Q1/6834 , C12Q1/6874 , C12Q1/6876 , C12Q2563/179 , C12Q2563/149 , C12Q2539/103 , C12Q2537/143 , C12Q2525/155 , C12Q2525/131
摘要： The present invention provides oligonucleotide constructs, sets of such oligonucleotide constructs, and methods of using such oligonucleotide constructs to provide validated sequences or sets of validated sequences corresponding to desired ROIs. Such validated ROIs and constructs containing these have a wide variety of uses, including in synthetic biology, quantitative nucleic acid analysis, polymorphism and/or mutation screening, and the like.
摘要翻译：本发明提供寡核苷酸构建体，这样的寡核苷酸构建体的集合，以及使用这样的寡核苷酸构建体来提供与期望的ROI相对应的验证序列或经验证序列的方法。这些验证的ROI和包含它们的构建体具有广泛的用途，包括合成生物学，定量核酸分析，多态性和/或突变筛选等。

3. 发明申请

WO2005079357A9 NUCLEIC ACID REPRESENTATIONS UTILIZING TYPE IIB RESTRICTION ENDONUCLEASE CLEAVAGE PRODUCTS 审中-公开
标题翻译：使用IIB型限制性内切酶产品的核酸代表
公开(公告)号：WO2005079357A9
公开(公告)日：2007-11-01
申请号：PCT/US2005004571
申请日：2005-02-15
申请人： DANA FARBER CANCER INST INC , MEYERSON MATTHEW L , TENGS TORSTEIN
发明人： MEYERSON MATTHEW L , TENGS TORSTEIN
IPC分类号： C12Q1/68 , C12N15/11
CPC分类号： C12Q1/6806 , C12N15/1093 , C12N15/66 , C12Q2539/103 , C12Q2521/313
摘要： This invention encompasses nucleic acid libraries comprising Type IIB restriction endonuclease cleavage products, or tags, including concatenated tags, and using concatenated and single Type IIB restriction endonuclease cleavage tags in diverse methods including karyotyping, pathogen discovery, identification of novel genes, subtraction techniques and transcript profiling.
摘要翻译：本发明包括包含IIB限制性内切核酸酶切割产物或包括连接标签的标签的核酸文库，并且使用包括核型分析，病原体发现，新基因鉴定，减法技术和转录物在内的不同方法中的连接和单一IIB型限制性内切酶切割标签剖析。

4. 发明申请

WO00029621A2 METHOD FOR MEASURING TARGET POLYNUCLEOTIDES AND NOVEL ASTHMA BIOMOLECULES 审中-公开
标题翻译：用于测量目标多核苷酸和新型ASTHMA生物分子的方法
公开(公告)号：WO00029621A2
公开(公告)日：2000-05-25
申请号：PCT/US1999/026931
申请日：1999-11-12
IPC分类号： C07K14/52 , C12Q1/68
CPC分类号： C12Q1/6883 , C07K14/521 , C12Q1/6809 , C12Q1/683 , C12Q2600/158 , C12Q2539/103
摘要： The present invention provides a method for simultaneously determining the levels of selected target polynucleotide sequences in a sample. In the method, selected target sequences are amplified using sequence-selective primer pairs to form double-stranded copies. The copies are cleaved with one or more endonucleases, and first and second aliquots of the cleaved fragment mixture are ligated to first and second adaptors, respectively, to form mixtures of first and second adaptor-fragment conjugates. The aliquots are combined and ligated to form conjugate dimers containing a conjugate from each of the first and second conjugate mixtures. After optional amplification, the conjugates are treated to release the adaptor segments, yielding target-identifier dimers. The dimers are polymerized to form target-identifier dimer multimers, and the relative abundances of the target-identifiers in one or more dimer multimers to provide an estimate of the levels of the selected target sequences in the sample. The present invention is particularly useful for determining expression levels of mRNA gene sequences in a sample, and for evaluating changes in mRNA expression levels among different samples or in response to changes in sample conditions. Also disclosed are certain polynucleotides and polypeptides that are useful for a variety of applications, particularly relating to asthma.
摘要翻译：本发明提供了一种用于同时测定样品中所选择的靶多核苷酸序列的水平的方法。在该方法中，使用序列选择性引物对扩增选择的靶序列以形成双链拷贝。用一种或多种内切核酸酶切割拷贝，将分离的片段混合物的第一和第二等分试样分别连接到第一和第二衔接子，以形成第一和第二衔接子 - 片段缀合物的混合物。将等分试样合并并连接以形成含有来自第一和第二共轭物混合物中的每一种的缀合物的共轭二聚体。在任选扩增后，处理缀合物以释放适配器片段，产生靶标识号二聚体。聚合二聚体以形成靶标识二聚体多聚体，以及一个或多个二聚体多聚体中靶标识别符的相对丰度，以提供样品中所选靶序列水平的估计。本发明特别可用于确定样品中mRNA基因序列的表达水平，以及用于评估不同样品中mRNA表达水平的变化或响应样品条件变化。还公开了可用于各种应用，特别是与哮喘有关的某些多核苷酸和多肽。

5. 发明申请

WO00024940A1 CELLULAR ARRAYS AND METHODS OF DETECTING AND USING GENETIC DISORDER MARKERS 审中-公开
标题翻译：细胞阵列和检测和使用遗传病标记的方法
公开(公告)号：WO00024940A1
公开(公告)日：2000-05-04
申请号：PCT/US1999/025370
申请日：1999-10-28
IPC分类号： C12N15/09 , B01L3/00 , C12M1/00 , G01N1/08 , G01N1/28 , G01N1/31 , G01N1/36 , G01N33/50 , G01N33/543 , G02B21/34 , C12Q1/68
CPC分类号： C12Q1/6886 , B01J2219/00659 , B01J2219/00702 , B01J2219/00743 , B01L3/5085 , C12Q1/6809 , C12Q1/6837 , C12Q1/6841 , C12Q2600/106 , C12Q2600/112 , C12Q2600/118 , C12Q2600/156 , G01N1/08 , G01N1/2813 , G01N1/312 , G01N33/5005 , G01N33/54306 , G01N2001/282 , G01N2001/288 , G01N2001/368 , G02B21/34 , C12Q2543/10 , C12Q2539/103 , C12Q2537/157 , C12Q2565/501
摘要： A method is disclosed for rapid molecular profiling of tissue or other cellular specimens by placing a donor specimen in an assigned location in a recipient array, providing copies of the array, and performing a different biological analysis of each copy. The results of the different biological analyses are compared to determine if there are correlations between the results of the different biological analyses at each assigned location. In some embodiment, the specimens may be tissue specimens from different tumors, which are subjected to multiple parallel molecular (including genetic and immunological) analyses. The results of the parallel analyses are then used to detect common molecular characteristics of the genetic disorder type, which can subsequently be used in the diagnosis or treatment of the disease. The biological characteristics of the tissue can be correlated with clinical or other information, to detect characteristics associated with the tissue, such as susceptibility or resistance to particular types of drug treatment. Other examples of suitable tissues which can be placed in the matrix include tissue from transgenic or model organisms, or cellular suspensions (such as cytological preparations or specimens of liquid malignancies or cell lines).
摘要翻译：公开了一种用于通过将供体样本放置在受体阵列中的分配位置以提供阵列的拷贝以及对每个拷贝执行不同生物学分析的组织或其它细胞标本的快速分子分析的方法。比较不同生物分析的结果，以确定每个分配位置的不同生物分析结果之间是否存在相关性。在一些实施方案中，样品可以是来自不同肿瘤的组织标本，其经受多重平行分子（包括遗传和免疫）分析。然后并行分析的结果用于检测遗传病症类型的常见分子特征，随后可用于诊断或治疗疾病。组织的生物学特征可以与临床或其他信息相关联，以检测与组织相关的特征，例如对特定类型的药物治疗的易感性或抗性。可以放置在基质中的合适组织的其他实例包括来自转基因或模型生物体的细胞或细胞悬浮液（例如细胞学制剂或液体恶性肿瘤或细胞系的标本）。

6. 发明申请

WO2014108850A2 HIGH THROUGHPUT TRANSCRIPTOME ANALYSIS 审中-公开
标题翻译：高通量转录分析
公开(公告)号：WO2014108850A2
公开(公告)日：2014-07-17
申请号：PCT/IB2014/058153
申请日：2014-01-09
申请人： YEDA RESEARCH AND DEVELOPMENT CO. LTD.
发明人： JAITIN, Diego , AMIT, Ido , KEREN-SHAUL, Hadas
IPC分类号： C12N15/10
CPC分类号： C12N15/1065 , C12N15/1096 , C12Q1/6806 , C12Q1/6809 , C12Q2521/119 , C12Q2521/501 , C12Q2525/143 , C12Q2525/173 , C12Q2539/103 , C12Q2563/179
摘要： Kits and methods for single cell or multiple cell transcriptome analysis are provided. An adapter polynucleotide is disclosed which comprises a double- stranded DNA portion of 15 base pairs and no more than 100 base pairs with a 3'single stranded overhang of at least 3 bases and no more than 10 bases, wherein said double stranded DNA portion is at the 5' end of the polynucleotide and wherein the sequence of said 3'single stranded overhang is selected from the group consisting of SEQ ID NOs: 1-8 and 9, wherein the 5' end of the strand of said double-stranded DNA which is devoid of said 3'single stranded overhang comprises a free phosphate.
摘要翻译：提供单细胞或多细胞转录组分析的试剂盒和方法。公开了一种接头多核苷酸，其包含15个碱基对和不超过100个碱基对的双链DNA部分，其具有至少3个碱基和不超过10个碱基的3'单链突出端，其中所述双链DNA部分是在多核苷酸的5'端，并且其中所述3'单链突出端的序列选自SEQ ID NO：1-8和9，其中所述双链DNA链的5'端其中没有所述3'单链突出端包含游离磷酸盐。

7. 发明申请

WO2007142608A1 NUCLEIC ACID CONCATENATION 审中-公开
标题翻译：核酸定位
公开(公告)号：WO2007142608A1
公开(公告)日：2007-12-13
申请号：PCT/SG2007/000159
申请日：2007-06-04
申请人： AGENCY FOR SCIENCE, TECHNOLOGY AND RESEARCH , RUAN, Yijun , NG, Patrick Wei Pern , FULLWOOD, Melissa Jane , LEE, Yen Ling
发明人： RUAN, Yijun , NG, Patrick Wei Pern , FULLWOOD, Melissa Jane , LEE, Yen Ling
IPC分类号： C12Q1/68 , C12N15/10
CPC分类号： C12Q1/6804 , C12Q1/6809 , C12Q2525/151 , C12Q2525/131 , C12Q2539/103
摘要： The present invention provides a method of manipulating nucleic acids. In particular, of length-controlled concatenating of nucleotide fragments, the method comprising: (a) providing at least two nucleotide fragments, wherein each fragment has one ligatable end and one non-ligatable end; and (b) allowing the two fragments to ligate at the ligatable ends to form an oligonucleotide comprising at least two concatenated nucleotide fragments. The present invention also provides an isolated oligonucleotide comprising at least two nucleotide fragments, wherein each fragment has at least one ligatable end and one non-ligatable end, and the fragments are ligated at the ligatable ends to form the oligonucleotide.
摘要翻译：本发明提供了一种操纵核酸的方法。特别地，长度控制的核苷酸片段连接，所述方法包括：（a）提供至少两个核苷酸片段，其中每个片段具有一个可连接末端和一个不可连接末端; 和（b）允许两个片段在可连接的末端连接以形成包含至少两个连接的核苷酸片段的寡核苷酸。本发明还提供了包含至少两个核苷酸片段的分离的寡核苷酸，其中每个片段具有至少一个可连接末端和一个不可连接的末端，并且将片段在可连接末端连接以形成寡核苷酸。

8. 发明申请

WO2006069346A3 METHYLATION-SENSITIVE RESTRICTION ENZYME ENDONUCLEASE METHOD OF WHOLE GENOME METHYLATION ANALYSIS 审中-公开
标题翻译：全基因组甲基化分析的甲基敏感性限制酶内切酶
公开(公告)号：WO2006069346A3
公开(公告)日：2006-08-31
申请号：PCT/US2005046896
申请日：2005-12-20
申请人： ILLUMINA INC , GUNDERSON KEVIN
发明人： GUNDERSON KEVIN
IPC分类号： C12Q1/68 , C12P19/34
CPC分类号： C12Q1/6827 , C12Q2521/331 , C12Q2521/101 , C12Q2539/103
摘要： The invention provides methods of identifying a plurality of reactive recognition sites for a restriction endonuclease in genomic DNA. In particular embodiments, the methods can be used to identify methylation state of a plurality of CpG target sites in genomic DNA. The method can include steps of treating genomic DNA with a restriction endonuclease, thereby producing genomic DNA fragments; ligating the fragments, thereby forming a concatenated DNA; and identifying sequence portions of the concatenated DNA that are re-ordered compared to the genomic DNA.
摘要翻译：本发明提供鉴定基因组DNA中限制性内切核酸酶的多个反应性识别位点的方法。在具体实施方案中，该方法可用于鉴定基因组DNA中多个CpG靶位点的甲基化状态。该方法可以包括用限制性内切核酸酶处理基因组DNA的步骤，从而产生基因组DNA片段; 连接片段，从而形成连接的DNA; 以及鉴定与基因组DNA相比重新排序的连接DNA的序列部分。

9. 发明申请

WO2004024953A1 METHOD FOR PREPARATION OF CDNA TAGS FOR IDENTIFYING EXPRESSED GENES AND METHOD FOR ANALYSIS OF GENE EXPRESSION 审中-公开
标题翻译：用于鉴定用于鉴定表达基因的CDNA标签的方法和用于分析基因表达的方法
公开(公告)号：WO2004024953A1
公开(公告)日：2004-03-25
申请号：PCT/JP2003/009901
申请日：2003-08-05
申请人： KUREHA CHEMICAL INDUSTRY COMPANY, LIMITED , YAMAMOTO, Mikio , YAMAMOTO, Naoki , HIROSE, Kunitaka , SAKAI, Jun
发明人： YAMAMOTO, Mikio , YAMAMOTO, Naoki , HIROSE, Kunitaka , SAKAI, Jun
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6809 , C12N15/1096 , C12N15/66 , C12Q1/6855 , C12Q2539/103 , C12Q2521/313
摘要： There are provided a method for the preparation of cDNA tags for identifying expressed genes and a method for analysis of gene expression. The cDNA tags for identifying expressed genes are prepared by the method comprising a combination of one type II restriction enzyme and three kinds of type IIS restriction enzymes, and linker X having recognition sites for first and second IIS restriction enzymes and linker Y having a recognition site for a third type IIS restriction enzyme. The cDNA tags can be used alone or in combination like chain (concatemer) formed by combining process to efficiently analyze gene expression.
摘要翻译：提供了用于鉴定表达基因的cDNA标签的制备方法和基因表达分析方法。用于鉴定表达基因的cDNA标签通过包含一种II型限制酶和三种类型的IIS限制酶的组合的方法制备，以及具有第一和第二IIS限制酶的识别位点的接头X和具有识别位点的接头Y 第三种类型的IIS限制酶。 cDNA标签可以单独或组合使用，如通过组合方法形成的链（连接体），以有效分析基因表达。

10. 发明申请

WO0208461A2 METHODS FOR ANALYSIS AND IDENTIFICATION OF TRANSCRIBED GENES, AND FINGERPRINTING 审中-公开
标题翻译：用于分析和鉴定转录基因的方法和指纹
公开(公告)号：WO0208461A2
公开(公告)日：2002-01-31
申请号：PCT/IB0101539
申请日：2001-07-23
申请人： GLOBAL GENOMICS AB , LINNARSSON STEN , ERNFORS PATRIK , BAUREN GORAN
发明人： LINNARSSON STEN , ERNFORS PATRIK , BAUREN GORAN
IPC分类号： C12N15/09 , C12N15/10 , C12Q1/68 , G06F19/20 , G06F19/24
CPC分类号： G06F19/20 , C12N15/1096 , C12Q1/6809 , G06F19/24 , Y02A90/24 , C12Q2539/103
摘要： A method for identifying mRNA molecules present in a sample, and also for quantifying the expression levels of the mRNA molecules. A profile of gene identities and/or expression levels is produced by generating two independent patterns characteristic of the population of mRNA molecules expressed in the sample and analysing these patterns using a combinatorial algorithm. Gene expression by different cell types or of the same cell types under different conditions may be compared. In this way, genes may be identified which play a role in determining various cellular processes and states, including susceptibility to external factors, development, and disease.
摘要翻译：用于鉴定样品中存在的mRNA分子的方法，以及用于定量mRNA分子的表达水平的方法。通过产生在样品中表达的mRNA分子的特征特征的两个独立模式并使用组合算法分析这些模式来产生基因特征和/或表达水平的谱。可以比较不同细胞类型或不同条件下相同细胞类型的基因表达。以这种方式，可以鉴定在确定各种细胞过程和状态中起作用的基因，包括对外部因素的易感性，发育和疾病。

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