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1. 发明申请

WO2004109289A1 METHOD OF DIAGNOSING SARS CORONA VIRUS INFECTION 审中-公开
标题翻译：诊断SARS CORONA病毒感染的方法
公开(公告)号：WO2004109289A1
公开(公告)日：2004-12-16
申请号：PCT/SG2004/000174
申请日：2004-06-10
申请人： AGENCY FOR SCIENCE, TECHNOLOGY AND RESEARCH , TAN, Yee-Joo , GOH, Phuay-Yee , FIELDING, Burtram, Clinton , SHEN, Shuo , LIM, Seng, Gee , HONG, Wan, Jin , RUAN, Yijun , WEI, Chia, Lin
发明人： TAN, Yee-Joo , GOH, Phuay-Yee , FIELDING, Burtram, Clinton , SHEN, Shuo , LIM, Seng, Gee , HONG, Wan, Jin , RUAN, Yijun , WEI, Chia, Lin
IPC分类号： G01N33/68
CPC分类号： G01N33/56983 , C07K16/10 , G01N2333/165
摘要： The present invention relates to methods of detecting the presence or absence of antibody to SARS coronavirus in a sample, based on the discovery that the S, M, E, N and U274 proteins of SARS coronavirus are antigenic. Such methods may be used to diagnose whether a patient has been infected by or exposed to SARS coronavirus. Antibodies directed against S, M, E, N and U274 proteins of SARS are also provided.
摘要翻译：基于SARS冠状病毒的S，M，E，N和U274蛋白质是抗原性的发现，本发明涉及检测样品中SARS冠状病毒抗体的存在或不存在的方法。这种方法可用于诊断患者是否已被SARS冠状病毒感染或暴露于SARS冠状病毒。还提供针对SARS的S，M，E，N和U274蛋白的抗体。

2. 发明申请

WO2004050918A1 METHOD TO GENERATE OR DETERMINE NUCLEIC ACID TAGS CORRESPONDING TO THE TERMINAL ENDS OF DNA MOLECULES USING SEQUENCES ANALYSIS OF GENE EXPRESSION (TERMINAL SAGE) 审中-公开
标题翻译：使用序列分析基因表达（终端信号）生成或确定与DNA分子末端相关的核酸标签的方法
公开(公告)号：WO2004050918A1
公开(公告)日：2004-06-17
申请号：PCT/SG2003/000255
申请日：2003-12-04
申请人： AGENCY FOR SCIENCE, TECHNOLOGY AND RESEARCH , RUAN, Yijun , WEI, Chialin
发明人： RUAN, Yijun , WEI, Chialin
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6809 , C12Q2539/103
摘要： We describe a method of providing an indication of an instance of expression of a gene, the method comprising the steps of (a) providing a complementary deoxyribonucleic acid (cDNA) having a terminus comprising a terminal transcribed sequence of a gene; (b) linking the cDNA to an linker sequence thereby forming a linked nucleic acid, in which the linker sequence comprises a first recognition site for a first nucleic acid cleavage enzyme, preferably a restriction endonuclease, that allows nucleic acid cleavage at a site distant from the first recognition site; and (c) cleaving the linked nucleic acid with the first nucleic acid cleavage enzyme to provide a linked tag, in which the linked tag comprises a nucleotide sequence tag representative of a terminal transcribed sequence of the gene; and (d) detecting the presence or identity of the linked tag or the nucleotide sequence tag to provide an indication of an instance of gene expression.
摘要翻译：我们描述了提供基因表达实例的指示的方法，所述方法包括以下步骤：（a）提供具有包含基因的末端转录序列的末端的互补脱氧核糖核酸（cDNA）; （b）将cDNA连接到接头序列，从而形成连接的核酸，其中接头序列包含用于第一核酸切割酶的第一识别位点，优选限制性内切核酸酶，其允许在远离的位点进行核酸切割第一个认可网站; 和（c）用第一核酸切割酶切割连接的核酸以提供连接的标签，其中连接的标签包含代表该基因的末端转录序列的核苷酸序列标签; 和（d）检测连锁标签或核苷酸序列标签的存在或身份以提供基因表达实例的指示。

3. 发明申请

WO2007106047A8 NUCLEIC ACID INTERACTION ANALYSIS 审中-公开
标题翻译：核酸相互作用分析
公开(公告)号：WO2007106047A8
公开(公告)日：2009-07-23
申请号：PCT/SG2007000069
申请日：2007-03-09
申请人： AGENCY SCIENCE TECH & RES , RUAN YIJUN , FULLWOOD MELISSA JANE , WEI CHIA LIN
发明人： RUAN YIJUN , FULLWOOD MELISSA JANE , WEI CHIA LIN
IPC分类号： C12N15/00 , C12N15/66 , C12Q1/68
CPC分类号： C12N15/1065 , C12Q1/6804 , C12Q2525/197 , C12Q2525/131 , C12Q2521/313 , C12Q2531/113 , C12Q2525/191 , C12Q2523/101
摘要： The present invention provides an isolated oligonucleotide and a method using the isolated oligonucleotide to detect and/or identify at least two polynucleotides from a nucleic acid-protein complex. The oligonucleotide comprises at least one first tag and at least one second tag, wherein the first and second tags are obtained from a nucleic acid-protein complex.
摘要翻译：本发明提供分离的寡核苷酸和使用分离的寡核苷酸从核酸 - 蛋白复合物检测和/或鉴定至少两种多核苷酸的方法。寡核苷酸包含至少一个第一标签和至少一个第二标签，其中第一个和第二个标签是从核酸 - 蛋白质复合物获得的。

4. 发明申请

WO2006031204A1 GENE IDENTIFICATION SIGNATURE (GIS) ANALYSIS FOR TRANSCRIPT MAPPING 审中-公开
标题翻译：基因识别签名（GIS）分析用于转录映射
公开(公告)号：WO2006031204A1
公开(公告)日：2006-03-23
申请号：PCT/SG2005/000281
申请日：2005-08-17
申请人： AGENCY FOR SCIENCE, TECHNOLOGY AND RESEARCH , SUNG, Wing Kin Ken , RUAN, Yijun
发明人： SUNG, Wing Kin Ken , RUAN, Yijun
IPC分类号： C12Q1/68
CPC分类号： G06F19/18 , G06F19/22
摘要： A transcript mapping method according to an embodiment of the invention is described hereinafter and combines short tag based (SAGE and MPSS) efficiency with the accuracy of full-length cDNA (flcDNA) for comprehensive characterization of transcriptomes. This method is also referred to as Gene Identification Signature (GIS) analysis. In this method, the 5' and 3' ends of full-length cDNA clones are initially extracted into a ditag structure, with the ditag concatemers of the ditag being subsequently sequenced in an efficient manner, and finally mapped to the genome for defining the gene structure. As a GIS ditag represents the 5' and 3' ends of a transcript, it is more informative than SAGE and MPSS tags. Segment lengths between 5' and 3' tag pairs are obtainable including orientation, ordering and chromosome family for efficient transcript mapping and gene location identification. Furthermore, a compressed suffix array (CSA) is used for indexing the genome sequence for improve mapping speed and to reduce computational memory requirements.
摘要翻译：下文描述了根据本发明实施方案的转录本映射方法，并将基于短标签（SAGE和MPSS）的效率与全长cDNA（flcDNA）的准确度相结合，用于全面表征转录组。这种方法也被称为基因识别签名（GIS）分析。在该方法中，全长cDNA克隆的5'和3'末端最初被提取到ditag结构中，ditag的ditag连接体随后以有效的方式进行测序，并且最终映射到用于定义基因的基因组结构体。作为一个地理信息系统地图代表了抄本的5'和3'端，它比SAGE和MPSS标签更具信息。可以获得5'和3'标签对之间的片段长度，包括定向，排列和染色体家族，用于有效的转录映射和基因位置识别。此外，压缩后缀阵列（CSA）用于索引基因组序列以提高映射速度并减少计算存储器要求。

5. 发明申请

WO2012082074A1 METHOD OF DETECTING RESISTANCE TO CANCER THERAPY 审中-公开
标题翻译：检测癌症治疗电阻的方法
公开(公告)号：WO2012082074A1
公开(公告)日：2012-06-21
申请号：PCT/SG2011/000437
申请日：2011-12-14
申请人： NATIONAL UNIVERSITY OF SINGAPORE , SINGAPORE HEALTH SERVICES PTE LTD , AGENCY FOR SCIENCE, TECHNOLOGY AND RESEARCH , RUAN, Yijun , ONG, Sin, Tiong , NG, King-Pan , CHUAH, Charles, Thuan, Heng , HILLMER, Axel, Maximilian , JUAN, Wen, Chun
发明人： RUAN, Yijun , ONG, Sin, Tiong , NG, King-Pan , CHUAH, Charles, Thuan, Heng , HILLMER, Axel, Maximilian , JUAN, Wen, Chun
IPC分类号： C12Q1/68 , C12N15/11
CPC分类号： C12Q1/6886 , C12Q2600/106 , C12Q2600/156
摘要： We describe a polymorphic variant of a BIM {BCL2L11) gene which comprises, in 5' to 3' order, the nucleotide sequence set out in SEQ ID NO: 5 followed immediately by the nucleotide sequence set out in SEQ ID NO: 7. The BIM polymorphic variant may be characterised by lacking the nucleotide sequence set out in SEQ ID NO: 6. It may be used to detect BCR-ABL-independent TKI-resistance (resistance to treatment with tyrosine kinase inhibitors) for chronic myelogenous leukaemia, c-KIT/PDGFR-independent TKI-resistance for gastrointestinal stromal tumours (GIST), EGFR-independent TKI-resistance for non-small cell lung cancer (NSCLC) or JAK2-independent TKI-resistance for a myeloproliferative disorder, in an individual comprising such a polymorphism.
摘要翻译：我们描述了BIM {BCL2L11）基因的多态性变体，其以5'至3'的顺序包含SEQ ID NO：5所示的核苷酸序列，然后紧接着SEQ ID NO：7所示的核苷酸序列。 BIM多态变体的特征可能是缺乏SEQ ID NO：6所示的核苷酸序列。它可用于检测慢性粒细胞性白血病，c-型BCR-ABL依赖性TKI抗性（用酪氨酸激酶抑制剂治疗的抗性）用于胃肠道间质瘤（GIST）的KIT / PDGFR非依赖性TKI-抗性，用于非小细胞肺癌（NSCLC）的EGFR非依赖性TKI-抗性或用于骨髓增生性疾病的独立于JAK2的TKI-抗性，其包含这样的多态性。

6. 发明申请

WO2007142608A1 NUCLEIC ACID CONCATENATION 审中-公开
标题翻译：核酸定位
公开(公告)号：WO2007142608A1
公开(公告)日：2007-12-13
申请号：PCT/SG2007/000159
申请日：2007-06-04
申请人： AGENCY FOR SCIENCE, TECHNOLOGY AND RESEARCH , RUAN, Yijun , NG, Patrick Wei Pern , FULLWOOD, Melissa Jane , LEE, Yen Ling
发明人： RUAN, Yijun , NG, Patrick Wei Pern , FULLWOOD, Melissa Jane , LEE, Yen Ling
IPC分类号： C12Q1/68 , C12N15/10
CPC分类号： C12Q1/6804 , C12Q1/6809 , C12Q2525/151 , C12Q2525/131 , C12Q2539/103
摘要： The present invention provides a method of manipulating nucleic acids. In particular, of length-controlled concatenating of nucleotide fragments, the method comprising: (a) providing at least two nucleotide fragments, wherein each fragment has one ligatable end and one non-ligatable end; and (b) allowing the two fragments to ligate at the ligatable ends to form an oligonucleotide comprising at least two concatenated nucleotide fragments. The present invention also provides an isolated oligonucleotide comprising at least two nucleotide fragments, wherein each fragment has at least one ligatable end and one non-ligatable end, and the fragments are ligated at the ligatable ends to form the oligonucleotide.
摘要翻译：本发明提供了一种操纵核酸的方法。特别地，长度控制的核苷酸片段连接，所述方法包括：（a）提供至少两个核苷酸片段，其中每个片段具有一个可连接末端和一个不可连接末端; 和（b）允许两个片段在可连接的末端连接以形成包含至少两个连接的核苷酸片段的寡核苷酸。本发明还提供了包含至少两个核苷酸片段的分离的寡核苷酸，其中每个片段具有至少一个可连接末端和一个不可连接的末端，并且将片段在可连接末端连接以形成寡核苷酸。

7. 发明申请

WO2007106047A1 NUCLEIC ACID INTERACTION ANALYSIS 审中-公开
标题翻译：核酸相互作用分析
公开(公告)号：WO2007106047A1
公开(公告)日：2007-09-20
申请号：PCT/SG2007/000069
申请日：2007-03-09
申请人： AGENCY FOR SCIENCE, TECHNOLOGY AND RESEARCH , RUAN, Yijun , FULLWOOD, Melissa Jane , WEI, Chia Lin
发明人： RUAN, Yijun , FULLWOOD, Melissa Jane , WEI, Chia Lin
IPC分类号： C12N15/00 , C12N15/66 , C12Q1/68
CPC分类号： C12N15/1065 , C12Q1/6804 , C12Q2525/197 , C12Q2525/131 , C12Q2521/313 , C12Q2531/113 , C12Q2525/191 , C12Q2523/101
摘要： The present invention provides an isolated oligonucleotide and a method using the isolated oligonucleotide to detect and/or identify at least two polynucleotides from a nucleic acid-protein complex. The oligonucleotide comprises at least one first tag and at least one second tag, wherein the first and second tags are obtained from a nucleic acid-protein complex.
摘要翻译：本发明提供分离的寡核苷酸和使用分离的寡核苷酸从核酸 - 蛋白复合物检测和/或鉴定至少两种多核苷酸的方法。所述寡核苷酸包含至少一个第一标签和至少一个第二标签，其中所述第一标签和第二标签是从核酸 - 蛋白质复合物获得的。

8. 发明申请

WO2006135342A1 METHOD OF PROCESSING AND/OR GENOME MAPPING OF DITAG SEQUENCES 审中-公开
标题翻译： DITAG序列的处理和/或基因组映射的方法
公开(公告)号：WO2006135342A1
公开(公告)日：2006-12-21
申请号：PCT/SG2006/000151
申请日：2006-06-12
申请人： AGENCY FOR SCIENCE, TECHNOLOGY AND RESEARCH , CHIU, Kuo Ping , RUAN, Yijun , WEI, Chia Lin
发明人： CHIU, Kuo Ping , RUAN, Yijun , WEI, Chia Lin
IPC分类号： G06F17/30 , C12Q1/68 , G06F19/00 , C12N15/00 , G01N33/48
CPC分类号： G06F19/28 , G06F19/22
摘要： There is provided a method and system for processing and/or mapping ditag nucleotide sequence(s) to a genome, the ditag sequence comprising the 5' terminal tag and the 3' terminal tag of a nucleic acid molecule or fragment thereof or genomic fragment. The method of processing comprises preparing a database or file comprising at least one ditag sequence. The method of mapping comprises preparing a database or file of ditag(s), and mapping the ditag sequence(s) to the genome, comprising matching the 5' and the 3' terminal tags of the ditag sequence to at least a portion of the genome.
摘要翻译：提供了用于处理和/或将基因组的核苷酸序列映射到基因组的方法和系统，包括核酸分子或其片段的5'末端标签和3'末端标签的基因组序列或基因组片段。所述处理方法包括准备包含至少一个ditag序列的数据库或文件。映射方法包括准备数据库或文件的一个或多个ditag序列，并将该ditag序列映射到基因组，包括将ditag序列的5'和3'末端标签与至少一部分基因组。

9. 发明申请

WO2005026391A1 METHOD FOR GENE IDENTIFICATION SIGNATURE (GIS) ANALYSIS 审中-公开
标题翻译：基因识别符号（GIS）分析方法
公开(公告)号：WO2005026391A1
公开(公告)日：2005-03-24
申请号：PCT/SG2004/000298
申请日：2004-09-16
申请人： AGENCY FOR SCIENCE, TECHNOLOGY AND RESEARCH , RUAN, Yijun , NG, Patrick , WEI, Chialin
发明人： RUAN, Yijun , NG, Patrick , WEI, Chialin
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6809 , C12N15/1065 , C12N15/1093 , C12N15/1096 , C12Q1/6855 , C12Q2525/191 , C12Q2525/131 , C12Q2521/313
摘要： An isolated oligonucleotide comprising at least one ditag, wherein the ditag comprises two joined first and second sequence tags, wherein the first tag comprises the 5'-terminus sequence and the second tag comprises the 3'-terminus sequence of a nucleic acid molecule or a fragment thereof. The ditag analysis is useful for gene discovery and genome mapping.
摘要翻译：包含至少一个ditag的分离的寡核苷酸，其中所述ditag包含两个连接的第一和第二序列标签，其中所述第一标签包含5'-末端序列，并且所述第二标签包含核酸分子的3'-末端序列或片段。 ditag分析可用于基因发现和基因组图谱。

10. 发明申请

WO2002095072A1 COLONY ARRAY-BASED cDNA LIBRARY NORMALIZATION BY HYBRIDIZATIONS OF COMPLEX RNA PROBES AND GENE SPECIFIC PROBES 审中-公开
标题翻译：通过复合RNA探针和基因特异性探针杂交的基于阵列的cDNA文库正常化
公开(公告)号：WO2002095072A1
公开(公告)日：2002-11-28
申请号：PCT/US2002/015113
申请日：2002-05-10
申请人： LARGE SCALE BIOLOGY CORPORATION , WEI, Chia-Lin , RUAN, Yijun , ZHENG, Wenjiin
发明人： WEI, Chia-Lin , RUAN, Yijun , ZHENG, Wenjiin
IPC分类号： C12Q1/68
CPC分类号： C12N15/1096
摘要： Each cell normally has a widely differing number of mRNA transcribed for each gene. Consequently, a fill-length cDNA library constructed from the mRNA would also have a widely differing number of cDNA for each gene. A normalized library of the full-length cDNA of a cell is usful for basic, applied, industrial, and medical research. This invention provides for a method for constructing a normalized full-length cDNA library by probing the members of a non-normalized cDNA library with a library of probes generated from mRNA in order to identigy the cDNA of genes that have low or high expression. A collection of the cDNA from the library of the genes that have low expression would constitute a normalized library of these genes. This invention also provided for a method to reduce the number cDNA of genes that have high expression represented by probing these cDNA with a library of probes generated from a small randomly selected number of these cDNA. cDNA that hybridize are represented within this small randomly selectes number of cDNA, while cDNA that do not hybridize are not represented. The latter cDNA can undergo further such probing to further reduce the number of cDNA represented. The cDNA from the library of the genes that have low expression and the randomly selected highly expressed cDNA would constitute a normalized library of these genes.
摘要翻译：每个细胞通常具有每个基因转录的mRNA数量差异很大。因此，由mRNA构建的填充长度cDNA文库对于每个基因也将具有大量不同数量的cDNA。细胞全长cDNA的归一化库对于基础，应用，工业和医学研究是有用的。本发明提供了通过用mRNA产生的探针文库探测非标准化cDNA文库的成员来构建归一化全长cDNA文库的方法，以便鉴定具有低表达或高表达的基因的cDNA。来自具有低表达的基因的文库的cDNA的集合将构成这些基因的归一化文库。本发明还提供了通过用随机选择数量小的这些cDNA产生的探针文库探索这些cDNA来减少具有高表达性的基因的cDNA数量的方法。在该小的随机选择数量的cDNA中表达杂交的cDNA，而不表示不杂交的cDNA。后一种cDNA可以进一步进行这种探测，以进一步减少所表达的cDNA的数量。来自低表达基因的文库和随机选择的高表达cDNA的cDNA将构成这些基因的归一化文库。

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