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1. 发明申请

WO2015015189A1 OSTEOINDUCTIVE MATERIALS 审中-公开
标题翻译：机械材料
公开(公告)号：WO2015015189A1
公开(公告)日：2015-02-05
申请号：PCT/GB2014/052322
申请日：2014-07-29
申请人： THE UNIVERSITY OF LIVERPOOL
发明人： CURRAN, Judith , HUNT, John
IPC分类号： A61L27/34
CPC分类号： A61L27/54 , A61C8/0013 , A61K31/131 , A61K31/695 , A61L27/10 , A61L27/18 , A61L27/28 , A61L27/3821 , A61L27/3847 , A61L27/50 , A61L31/08 , A61L31/14 , A61L2300/414 , A61L2400/12 , A61L2400/18 , A61L2430/02 , A61L2430/12 , C12N5/0654 , C12N5/0662 , C12N2506/1346 , C12N2533/00 , C12N2533/12 , C12N2533/30 , C12N2533/40
摘要： Provided is an osteoinductive material comprising a substrate; and a layer of a hydrocarbyl group comprising 10 to 12 carbon atoms, the hydrocarbyl group having one end attached to the substrate and a presenting amine (–NH2) group at the unattached end. The hydrocarbyl group may be 11 carbons long, and may be provided in the form of 11-aminoundecyltriethoxysilane. The substrate may be a glass; metal; polymer; ceramic; plastic; or hydroxyapatite. The substrate may, for example, be a metal selected from the group consisting of: titanium; titanium alloys; cobalt chrome; and steel. An osteoinductive material may be provided in the form of a medical implant, or part thereof, such as an orthopaedic implant or a dental implant or part thereof. Alternatively the substrate may be in the form of a cell culture vessel. Also provided is a method of producing such osteoinductive materials, the medical use of such materials, and a method of producing bone or a bone precursor utilising the disclosed materials.
摘要翻译：提供了包含基底的骨诱导材料; 和包含10至12个碳原子的烃基基团，其中一个末端连接到基底上的烃基和未连接末端的呈现胺（-NH 2）基团。烃基可以为11个碳原子，并且可以以11-氨基十九烷基三乙氧基硅烷的形式提供。基底可以是玻璃; 金属; 聚合物; 陶瓷; 塑料; 或羟基磷灰石。基材可以是例如选自钛的金属; 钛合金; 钴铬; 和钢铁。骨诱导材料可以以医疗植入物或其一部分的形式提供，例如矫形植入物或牙科植入物或其部分。或者，底物可以是细胞培养容器的形式。还提供了生产这种骨诱导材料的方法，这种材料的医学用途，以及利用所公开的材料制备骨骼或骨骼前体的方法。

2. 发明申请

WO2012166794A1 PATTERNING AND CELLULAR CO-CULTURE 审中-公开
标题翻译：文化与细胞文化
公开(公告)号：WO2012166794A1
公开(公告)日：2012-12-06
申请号：PCT/US2012/039991
申请日：2012-05-30
申请人： NANOINK, INC. , COLLINS, John, M. , NETTIKADAN, Saju, R.
发明人： COLLINS, John, M. , NETTIKADAN, Saju, R.
IPC分类号： C12N5/00 , G01N33/50
CPC分类号： C12N5/0068 , C12N2502/00 , C12N2533/12 , C12N2533/52 , C12N2535/10 , G01N2500/10
摘要： Cell co-culturing methods and arrays providing versatility and high resolution. A method comprising: providing at least one substrate; providing at least one first tip with at least one first cell-adhesion material disposed thereon, and at least one second tip with at least one second cell-adhesion material disposed thereon, wherein the first cell-adhesion material is different from the second cell-adhesion material; depositing the first cell-adhesion material from the first tip to the substrate to form at least one first deposit, and depositing the second cell-adhesion material from the second tip to the substrate to form at least one second deposit; and wherein the first and second deposits are capable of providing selective binding to at least one first cell so that the first cell selectively binds to the first deposit, and wherein the second deposit is capable of binding to at least one second cell. Single cell binding and cell interaction studies can be carried out.
摘要翻译：细胞共培养方法和阵列提供多功能性和高分辨率。一种方法，包括：提供至少一个基底; 提供至少一个第一尖端，其上设置有至少一个第一细胞粘附材料，以及至少一个第二尖端，其上设置有至少一个第二细胞粘附材料，其中所述第一细胞粘附材料与所述第二细胞粘附材料不同，粘合材料; 将第一细胞粘附材料从第一末端沉积到基底以形成至少一个第一沉积物，以及将第二细胞粘附材料从第二末端沉积到基底以形成至少一个第二沉积物; 并且其中所述第一和第二沉积物能够提供与至少一个第一细胞的选择性结合，使得所述第一细胞选择性地结合所述第一沉积物，并且其中所述第二沉积物能够结合至少一个第二细胞。可以进行单细胞结合和细胞相互作用研究。

3. 发明申请

WO2011088155A2 MATERIALS AND METHODS FOR PRODUCING CELL-SURFACE DIRECTED AND ASSOCIATED NON-NATURALLY OCCURRING BIOINORGANIC MEMBRANES AND USES THEREOF 审中-公开
标题翻译：用于生产细胞表面定向和相关的非天然发生的生物有机膜的材料和方法及其用途
公开(公告)号：WO2011088155A2
公开(公告)日：2011-07-21
申请号：PCT/US2011/021032
申请日：2011-01-12
申请人： PURDUE RESEARCH FOUNDATION , JAROCH, David Benjamin , RICKUS, Jenna Leigh
发明人： JAROCH, David Benjamin , RICKUS, Jenna Leigh
CPC分类号： C12N11/14 , C12N5/0012 , C12N5/0068 , C12N5/0677 , C12N2533/12
摘要： Materials and methods are provided for producing cell-surface directed, non-naturally occurring, bioinorganic membranes for association with the cell surfaces of living cells. The methods comprise exposing a cell to an acidic biomineralization buffer environment for cell-mediated deposition of the biomineral membrane onto the surface of the cell. The methods also comprise attaching a peptide, having a net positive charge under the acidic conditions, to the cell surface for serving as a template in directing the cell-mediated deposition of the biomineral membrane onto the surface of the cell.
摘要翻译：提供了用于产生与活细胞的细胞表面相关的细胞表面定向的，非天然存在的生物无机膜的材料和方法。该方法包括将细胞暴露于酸性生物矿化缓冲液环境中，用于细胞介导的生物矿物膜沉积到细胞表面上。所述方法还包括将在酸性条件下具有净正电荷的肽附着到细胞表面，用作指导细胞介导的生物矿物膜沉积到细胞表面上的模板。

4. 发明申请

WO2010007524A2 MATERIALS AND METHODS FOR CELL GROWTH 审中-公开
标题翻译：细胞生长的材料和方法
公开(公告)号：WO2010007524A2
公开(公告)日：2010-01-21
申请号：PCT/IB2009/006521
申请日：2009-07-10
申请人： ULIVE ENTERPRISES LIMITED , CURRAN, Judith, M. , GRAHAM, Duncan , HUNT, John, A. , STOKES, Robert
发明人： CURRAN, Judith, M. , GRAHAM, Duncan , HUNT, John, A. , STOKES, Robert
IPC分类号： C12M1/26 , C12N5/00
CPC分类号： C12N5/0068 , C12N5/0655 , C12N2533/12 , C12N2533/14 , C12N2535/10
摘要： Nanopatterned surfaces which provide for improved cell growth including improved stem cell differentiation. The patterned surfaces can comprise an array of fields of biologically active moieties and can be controlled by parameters which include the pitch between the fields and the size of the fields. Nanopatterning can be carried out with use of dip pen nanolithographic printing, microcontact printing, and nanoimprint lithography.
摘要翻译：提供改善的细胞生长的纳米图案表面，包括改进的干细胞分化。图案化表面可以包括生物活性部分的阵列阵列，并且可以通过包括场之间的间距和场的大小的参数来控制。可以使用浸笔纳米光刻印刷，微接触印刷和纳米压印光刻进行纳米图案化。

5. 发明申请

WO2008015682A3 METHODS OF EXPANDING EMBRYONIC STEM CELLS IN A SUSPENSION CULTURE 审中-公开
标题翻译：在悬浮培养中扩增胚胎干细胞的方法
公开(公告)号：WO2008015682A3
公开(公告)日：2008-07-31
申请号：PCT/IL2007000970
申请日：2007-08-02
申请人： TECHNION RES & DEV FOUNDATION , AMIT MICHAL , ITSKOVITZ-ELDOR JOSEPH
发明人： AMIT MICHAL , ITSKOVITZ-ELDOR JOSEPH
IPC分类号： C12N5/0735
CPC分类号： C12N5/0606 , C12N5/0031 , C12N5/0037 , C12N5/0043 , C12N2501/115 , C12N2501/15 , C12N2501/2306 , C12N2501/235 , C12N2533/12 , C12N2533/90
摘要： A method of expanding and maintaining human embryonic stem cells (ESCs) in an undifferentiated state by culturing the ESCs in a suspension culture under culturing conditions devoid of substrate adherence is provided. Also provided are a method of deriving ESC lines in the suspension culture and methods of generating lineage-specific cells from ESCs which were expanded in the suspension culture of the present invention.
摘要翻译：提供了通过在没有底物粘附的培养条件下在悬浮培养物中培养ESCs来扩增和维持处于未分化状态的人胚胎干细胞（ESCs）的方法。还提供了在悬浮培养物中得到ESC系的方法以及在本发明的悬浮培养物中扩增的ESC产生谱系特异性细胞的方法。

6. 发明申请

WO2007091255A2 METHOD AND APPARATUS FOR MAINTENANCE AND EXPANSION OF HEMATOPOIETIC STEM CELLS FROM MONONUCLEAR CELLS 审中-公开
标题翻译：用于维持和扩增单核细胞的HEMATOPOIETIC干细胞的方法和装置
公开(公告)号：WO2007091255A2
公开(公告)日：2007-08-16
申请号：PCT/IL2007/000157
申请日：2007-02-06
申请人： PLURISTEM LIFE SYSTEMS INC. , MERETZKI, Shai
发明人： MERETZKI, Shai
IPC分类号： A61K35/12 , C12N5/0789
CPC分类号： C12N5/0647 , A61K2035/124 , C12N2502/025 , C12N2502/1358 , C12N2502/1382 , C12N2502/1388 , C12N2533/12 , C12N2533/30 , C12N2533/32
摘要： A method of expanding/maintaining undifferentiated hematopoietic stem cells by obtaining unselected mononuclear cells; and either seeding the mononuclear cells into a stationary phase plug-flow bioreactor in which a three dimensional mesenchymal/stromal cell culture has been pre-established, thereby expanding/maintaining undifferentiated hematopoietic stem cells.
摘要翻译：通过获得未选择的单核细胞扩增/维持未分化的造血干细胞的方法; 并将单核细胞接种到已经预先建立三维间充质/间质细胞培养物的固定相插塞生物反应器中，从而扩增/维持未分化的造血干细胞。

7. 发明申请

WO2003068149A2 PREPARATIONS OF NUCLEUS PULPOSUS CELLS AND METHODS FOR THEIR GENERATION, IDENTIFICATION, AND USE 审中-公开
标题翻译：核桃细胞的制备及其产生，鉴定和使用的方法
公开(公告)号：WO2003068149A2
公开(公告)日：2003-08-21
申请号：PCT/US2003/003608
申请日：2003-02-07
申请人： THE TRUSTEES OF THE UNIVERSITY PENNSYLVANIA , SHAPIRO, Irving , RAJPUROHIT, Ramesh , DUCHEYNE, Paul
发明人： SHAPIRO, Irving , RAJPUROHIT, Ramesh , DUCHEYNE, Paul
IPC分类号： A61K
CPC分类号： C12N5/0655 , A61K35/12 , A61K35/28 , C12N2533/12 , C12N2533/40
摘要： The present invention is directed to novel compositions and methods for the treatment of degenerative intervertebral disc disease. In some embodiments, the invention relates to a preparation of nucleus pulposus cells comprising purified nucleus pulposus cells. In some embodiments, the invention relates to methods of treating degenerative intervertebral disc disease in an individual comprising implanting nucleus pulposus cells into the nucleus pulposus space of a degenerated disc of the individual. Other embodiments of the invention relate to methods of generating nucleus pulposus cells.
摘要翻译：本发明涉及用于治疗退行性椎间盘疾病的新型组合物和方法。在一些实施方案中，本发明涉及包含纯化髓核细胞的髓核细胞的制备物。在一些实施方案中，本发明涉及在个体中治疗退行性椎间盘疾病的方法，包括将髓核细胞植入个体退化椎间盘的髓核空间中。本发明的其它实施方案涉及产生髓核细胞的方法。

8. 发明申请

WO03010300A2 BIOMATERIAL SUBSTRATES 审中-公开
标题翻译：生物基质
公开(公告)号：WO03010300A2
公开(公告)日：2003-02-06
申请号：PCT/GB0202634
申请日：2002-06-11
申请人： UNIV LIVERPOOL , COUSINS BRIAN G , GARVEY MICHAEL JOSEPH , FINK JOHN , WILLIAMS RACHEL LUCINDA , DOHERTY PATRICK JOSEPH
发明人： COUSINS BRIAN G , GARVEY MICHAEL JOSEPH , FINK JOHN , WILLIAMS RACHEL LUCINDA , DOHERTY PATRICK JOSEPH
IPC分类号： A61L15/24 , A61L15/42 , A61L27/34 , A61L27/50 , C12N5/00 , C12N5/02 , A61L15/00 , A61L27/16
CPC分类号： C12N5/0068 , A61L15/24 , A61L15/42 , A61L27/34 , A61L27/50 , A61L2400/18 , C12N2533/12 , C12N2533/30 , C12N2535/10 , C08L27/18
摘要： The invention concerns the use of a biomaterial substrate to orient cell or tissue growth. The substrate comprises a base portion and a surface layer covering at least part of the base portion. The surface layer provides the substrate with topographical features having at least one nanoscale dimension of from about 1 to about 200 nm. The topographical features having the capacity to orient cell or tissue growth thereon and/or therebetween.
摘要翻译：本发明涉及使用生物材料底物来定向细胞或组织生长。基板包括基部和覆盖基部的至少一部分的表面层。表面层为基底提供具有约1至约200nm的至少一个纳米级尺寸的形貌特征。具有在其上和/或其间定向细胞或组织生长的能力的形貌特征。

9. 发明申请

WO1995024468A1 HEPATITIS A VIRUS CULTURE PROCESS 审中-公开
公开(公告)号：WO1995024468A1
公开(公告)日：1995-09-14
申请号：PCT/US1995002516
申请日：1995-03-03
申请人： MERCK & CO., INC. , LEU, Frank, S. , SEIFERT, Douglas, B.
发明人： MERCK & CO., INC.
IPC分类号： C12N05/00
CPC分类号： C12N7/00 , A61K39/12 , A61K39/165 , A61K39/25 , A61K39/29 , A61K2039/55555 , C12N5/0075 , C12N2531/00 , C12N2533/12 , C12N2710/16734 , C12N2760/18734 , C12N2770/32434 , C12N2770/32451 , Y02A50/464 , Y02A50/466
摘要： A microcarrier based process to produce viral vaccines, of which one example is Hepatitis A virus (HAV), is composed of an aggregated microcarrier system of glass coated polystyrene microcarriers and MRC-5 cells, which creates a stable environment for the propagation of the virus over even extended infection periods. The microcarrier aggregates formed according to this process eliminate the sloughing of cells from the beads during long cultivations seen in other systems, allowing high virus productivity in microcarrier culture. The methodology is applicable where virus production can be enhanced by creating a stable culture during an extended infection period. Scalable stirred bioreactors are used instead of multiple parallel stationary surface bioreactors. This aggregated microcarrier process eliminates the capacity limitations of stationary surface bioreactors, it protects the cells upon which virus is cultured from shear, it provides enhanced cell to cell interactions, it provides a stable environment for virus growth, it provides void space for the convective transport of nutrients through the aggregate, and it provides a straight forward method for harvesting the viral lysate for downstream processing.
摘要翻译：基于微载体的生产病毒疫苗的方法，其中一个例子是甲型肝炎病毒（HAV），由玻璃包被的聚苯乙烯微载体和MRC-5细胞的聚集的微载体系统组成，其为病毒的繁殖创造了稳定的环境甚至延长感染期。根据该方法形成的微载体聚集体在其他系统中观察到的长期培养期间消除了细胞从珠粒中的脱落，从而允许微载体培养物中的高病毒生产力。该方法适用于通过在延长的感染期间创建稳定的培养物来增强病毒生产。使用可伸缩的搅拌生物反应器代替多个平行的固定表面生物反应器。这种聚集的微载体过程消除了固定表面生物反应器的容量限制，它保护了病毒从剪切中培养的细胞，它提供增强的细胞与细胞的相互作用，它为病毒生长提供了稳定的环境，它为对流传递提供了空间的营养物质，并且它提供了一种用于收获用于下游处理的病毒裂解物的直接方法。

10. 发明申请

WO2014062505A1 IMPROVED RELIABILITY OF ASSAYS USING A MULTI-DIVOT PLATFORM AND MULTI-SOURCE, MULTI-CELL TYPE CLUSTERS 审中-公开
标题翻译：使用多重平台和多源多个类型的集群进行测试的改进的可靠性
公开(公告)号：WO2014062505A1
公开(公告)日：2014-04-24
申请号：PCT/US2013/064583
申请日：2013-10-11
申请人： THE UNIVERSITY OF KANSAS
发明人： STEHNO-BITTEL, Lisa , RAMACHANDRAN, Karthik , RAWAL, Sonia
IPC分类号： C12N5/00 , C12N5/071 , C12Q1/00 , A61K48/00 , A61P37/06 , A01N1/02
CPC分类号： C12N5/0677 , A01N1/0221 , A01N1/0284 , A61K35/28 , A61K35/39 , A61K2035/124 , C12N2502/1358 , C12N2502/22 , C12N2533/12 , C12N2535/00 , G01N33/5044 , G01N33/54393 , G01N33/74 , G01N2333/62
摘要： Described herein are 3-dimensional clusters of reaggregated cells comprising cells reaggregated from at least two different cell sources, such as different cell types, different donors, and combinations thereof. Methods of making, using, and cryopreserving these 3-dimensional clusters of reaggregated cells are also described herein.
摘要翻译：本文描述的是重组细胞的3维簇，其包含从至少两种不同细胞来源例如不同细胞类型，不同供体及其组合再聚集的细胞。本文还描述了制备，使用和冷冻保存这些再聚集细胞的三维簇的方法。

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