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1. 发明申请

WO2012047808A1 CELL ASSAY METHODS AND ARTICLES 审中-公开
标题翻译：细胞测定方法和文章
公开(公告)号：WO2012047808A1
公开(公告)日：2012-04-12
申请号：PCT/US2011/054627
申请日：2011-10-03
申请人： NANOINK, INC. , NETTIKADAN, Saju , COLLINS, John M. , SMETANA, Alexander B. , STILES, Paul Leon
发明人： NETTIKADAN, Saju , COLLINS, John M. , SMETANA, Alexander B. , STILES, Paul Leon
IPC分类号： G01N33/50
CPC分类号： G01N33/5008
摘要： Versatile, efficient cell assays which can be prepared with use of nanolithography and can be used to test nanomaterials, pharmaceuticals, toxins, and the like. For example, a method comprising: depositing at least one first composition comprising at least one cell adhesion material on at least one substrate to form a pattern which forms an interior space on the substrate within the pattern, depositing in the interior space on the substrate at least one second composition, different from the first, comprising at least one material adapted to affect or potentially affect cell function. Tip-based deposition and direct-write methods can be used for deposition at nanoscale and sub-cellular resolutions. Nanoscopic and atomic force microscope tips can be used. Multiplexing can be carried out.
摘要翻译：可以使用纳米光刻技术制备的多功能，高效的细胞测定法，可用于测试纳米材料，药物，毒素等。例如，一种方法包括：将至少一种包含至少一种细胞粘附材料的第一组合物沉积在至少一个基底上以形成在图案内的衬底上形成内部空间的图案，在衬底上的内部空间中沉积不同于第一组合物的至少一个第二组合物包含适于影响或潜在地影响细胞功能的至少一种材料。基于尖端的沉积和直写方法可用于以纳米尺度和亚细胞分辨率进行沉积。可以使用纳米镜和原子力显微镜尖端。可以进行多路复用。

2. 发明申请

WO2012166794A1 PATTERNING AND CELLULAR CO-CULTURE 审中-公开
标题翻译：文化与细胞文化
公开(公告)号：WO2012166794A1
公开(公告)日：2012-12-06
申请号：PCT/US2012/039991
申请日：2012-05-30
申请人： NANOINK, INC. , COLLINS, John, M. , NETTIKADAN, Saju, R.
发明人： COLLINS, John, M. , NETTIKADAN, Saju, R.
IPC分类号： C12N5/00 , G01N33/50
CPC分类号： C12N5/0068 , C12N2502/00 , C12N2533/12 , C12N2533/52 , C12N2535/10 , G01N2500/10
摘要： Cell co-culturing methods and arrays providing versatility and high resolution. A method comprising: providing at least one substrate; providing at least one first tip with at least one first cell-adhesion material disposed thereon, and at least one second tip with at least one second cell-adhesion material disposed thereon, wherein the first cell-adhesion material is different from the second cell-adhesion material; depositing the first cell-adhesion material from the first tip to the substrate to form at least one first deposit, and depositing the second cell-adhesion material from the second tip to the substrate to form at least one second deposit; and wherein the first and second deposits are capable of providing selective binding to at least one first cell so that the first cell selectively binds to the first deposit, and wherein the second deposit is capable of binding to at least one second cell. Single cell binding and cell interaction studies can be carried out.
摘要翻译：细胞共培养方法和阵列提供多功能性和高分辨率。一种方法，包括：提供至少一个基底; 提供至少一个第一尖端，其上设置有至少一个第一细胞粘附材料，以及至少一个第二尖端，其上设置有至少一个第二细胞粘附材料，其中所述第一细胞粘附材料与所述第二细胞粘附材料不同，粘合材料; 将第一细胞粘附材料从第一末端沉积到基底以形成至少一个第一沉积物，以及将第二细胞粘附材料从第二末端沉积到基底以形成至少一个第二沉积物; 并且其中所述第一和第二沉积物能够提供与至少一个第一细胞的选择性结合，使得所述第一细胞选择性地结合所述第一沉积物，并且其中所述第二沉积物能够结合至少一个第二细胞。可以进行单细胞结合和细胞相互作用研究。

3. 发明申请

WO2004027797A3 MAGNETIC SWITCH ASSEMBLY 审中-公开
标题翻译：磁性开关总成
公开(公告)号：WO2004027797A3
公开(公告)日：2004-05-06
申请号：PCT/US0328687
申请日：2003-09-10
申请人： MAGNASPHERE CORP , COLLINS JOHN M
发明人： COLLINS JOHN M
IPC分类号： H01H3/16 , H01H36/00 , H01H9/00
CPC分类号： H01H36/00 , H01H3/161 , H01H2036/0086
摘要： An improved magnetic switch (10) is provided which is designed for use in an alarm circuit (52) in order to detect relative movement between first and second members such as a door (14) and frame (12), so as to signal unauthorized opening of the door (14). The switch (10) includes a switch assembly (20) for mounting in frame (12) and having first and second switch elements (40, 42), an electrically conductive, shiftable body (44) adjacent the elements (40, 42), and a first attractive component (45). Additionally, the switch (10) has a second attractive component (22) for mounting to the door (14). In use when door (14) is closed and circuit (52) is armed, the magnetic attraction between body (44) and component (22) shifts the body (44) to a switch position where the body (44) is out of simultaneous contact with the switch elements (40, 42). If the door (14) is opened, the magnetic attraction between body (44) and component (45) moves the body to another switch position establishing simultaneous contact with the switch elements (40, 42).
摘要翻译：提供了一种改进的磁开关（10），其设计用于在报警电路（52）中使用，以便检测诸如门（14）和框架（12）之间的第一和第二构件之间的相对运动，以便发出未授权打开门（14）。开关（10）包括用于安装在框架（12）中并具有第一和第二开关元件（40,42）的开关组件（20），邻近元件（40,42）的导电的可移动本体（44）和第一有吸引力的部件（45）。另外，开关（10）具有用于安装到门（14）的第二吸引部件（22）。当门（14）关闭并且电路（52）被布防时，在使用中，主体（44）和部件（22）之间的磁吸引力将主体（44）移动到主体（44）不同时的开关位置与开关元件（40,42）接触。如果门（14）打开，则本体（44）和部件（45）之间的磁吸引力将主体移动到与开关元件（40,42）同时接触的另一开关位置。

4. 发明申请

WO2004027797A2 MAGNETIC SWITCH ASSEMBLY 审中-公开
标题翻译：磁性开关总成
公开(公告)号：WO2004027797A2
公开(公告)日：2004-04-01
申请号：PCT/US2003/028687
申请日：2003-09-10
申请人： MAGNASPHERE CORPORATION , COLLINS, John, M.
发明人： COLLINS, John, M.
IPC分类号： H01H
CPC分类号： H01H36/00 , H01H3/161 , H01H2036/0086
摘要： An improved magnetic switch (10) is provided which is designed for use in an alarm circuit (52) in order to detect relative movement between first and second members such as a door (14) and frame (12), so as to signal unauthorized opening of the door (14). The switch (10) includes a switch assembly (20) for mounting in frame (12) and having first and second switch elements (40, 42), an electrically conductive, shiftable body (44) adjacent the elements (40, 42), and a first attractive component (45). Additionally, the switch (10) has a second attractive component (22) for mounting to the door (14). In use when door (14) is closed and circuit (52) is armed, the magnetic attraction between body (44) and component (22) shifts the body (44) to a switch position where the body (44) is out of simultaneous contact with the switch elements (40, 42). If the door (14) is opened, the magnetic attraction between body (44) and component (45) moves the body to another switch position establishing simultaneous contact with the switch elements (40, 42).
摘要翻译：提供了一种改进的磁性开关（10），该磁性开关被设计用于在警报电路（52）中使用，以检测第一和第二构件（例如门（14）和框架（以便发出未经授权打开门（14）的信号。开关（10）包括用于安装在框架（12）中并具有第一和第二开关元件（40,42）的开关组件（20），与元件（40,42）相邻的导电可移位主体（44）和第一吸引组件（45）。另外，开关（10）具有用于安装到门（14）的第二吸引组件（22）。在门14被关闭并且电路52被装备时，在主体44和部件22之间的磁引力使主体44转换到主体44不同时在主体44上的开关位置时，接触开关元件（40,42）。如果门（14）打开，主体（44）和部件（45）之间的磁引力使主体移动到另一个开关位置，建立与开关元件（40,42）的同时接触。

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