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    • 2. 发明申请
    • DISPOSABLE DIRECT CAPTURE DEVICE
    • 可替代的直接捕获设备
    • WO2014120344A2
    • 2014-08-07
    • PCT/US2013/074857
    • 2013-12-13
    • EMD MILLIPORE CORPORATION
    • CACACE, Benjamin
    • G01N1/34B01J39/26B01J41/20B01J47/014B01J47/022B01J47/127B01L3/505B01L2200/12B01L2300/0681B01L2300/123C07K1/14C07K1/36G01N1/405
    • The invention relates to a liquid sample preparation device such as a disposable, collapsible, flexible polymeric bag containing an adsorptive curtain of a functionalized shaped polymeric fiber bed for the direct capture of biomolecules from liquid samples. The functionalized shaped polymeric fiber bed includes fibrillated, ridged or winged-shaped fiber structures that significantly increases the surface area of the fiber resulting in enhanced separation, retention and/or purification of liquid samples containing biomolecules of interest as the liquid samples contact the adsorptive curtain of functionalized shaped fibers. Liquid samples include unclarified liquid feeds or other liquids containing one or more biomolecules of interest, including, but not limited to vaccines, recombinant proteins, cells, stem cells, monoclonal antibodies (mAbs), proteins, antibody, peptides, oligopeptides, nucleic acids, oligonucleotides, RNA, DNA, oligosaccharides and polysaccharides.
    • 本发明涉及一种液体样品制备装置,例如一次性可折叠的柔性聚合物袋,其包含用于从液体样品直接捕获生物分子的官能化成形聚合物纤维床的吸附帘。 功能化的聚合物纤维床包括原纤化的,脊状的或翼状的纤维结构,其显着增加纤维的表面积,导致当液体样品接触吸附窗帘时,含有生物分子的液体样品的增强的分离,保留和/或纯化 的功能化成形纤维。 液体样品包括未澄清的液体进料或含有一种或多种感兴趣的生物分子的其它液体,包括但不限于疫苗,重组蛋白,细胞,干细胞,单克隆抗体(mAb),蛋白质,抗体,肽,寡肽,核酸, 寡核苷酸,RNA,DNA,寡糖和多糖。