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1. 发明申请

WO2013191793A1 NUCLEIC ACID SEQUENCING BY NANOPORE DETECTION OF TAG MOLECULES 审中-公开
标题翻译：通过纳米检测标签分子的核酸序列
公开(公告)号：WO2013191793A1
公开(公告)日：2013-12-27
申请号：PCT/US2013/035630
申请日：2013-04-08
申请人： THE TRUSTEES OF COLUMBIA UNIVERSITY IN THE CITY OF NEW YORK , GENIA TECHNOLOGIES, INC. , JU, Jingyue , DAVIS, Randall , CHEN, Roger
发明人： JU, Jingyue , DAVIS, Randall , CHEN, Roger
IPC分类号： C12Q1/68 , C12Q1/00
CPC分类号： C12Q1/6869 , G01N33/48721 , C12Q2521/101 , C12Q2521/543 , C12Q2537/157 , C12Q2565/631
摘要： This disclosure provides systems and methods for sequencing nucleic acids using nucleotide analogues and translocation of tags from incorporated nucleotide analogues through a nanopore. In aspects, this disclosure is related to composition, method, and system for sequencing a nucleic acid using tag molecules and detection of translocation through a nanopore of tags released from incorporation of the molecule.
摘要翻译：本公开提供了使用核苷酸类似物对核酸进行测序的系统和方法，以及通过纳米孔从掺入的核苷酸类似物转移标签。在一些方面，本公开涉及使用标签分子对核酸进行测序的组合物，方法和系统，以及通过从分子掺入释放的标记的纳米孔的易位检测。

2. 发明申请

WO2015148402A1 CHEMICAL METHODS FOR PRODUCING TAGGED NUCLEOTIDES 审中-公开
标题翻译：用于生产标记核酸的化学方法
公开(公告)号：WO2015148402A1
公开(公告)日：2015-10-01
申请号：PCT/US2015/022063
申请日：2015-03-23
申请人： THE TRUSTEES OF COLUMBIA UNIVERISTY IN THE CITY OF NEW YORK , GENIA TECHNOLOGIES, INC. , FULLER, Carl, W. , KUMAR, Shiv , JU, Jingyue , DAVIS, Randall , CHEN, Roger
发明人： FULLER, Carl, W. , KUMAR, Shiv , JU, Jingyue , DAVIS, Randall , CHEN, Roger
IPC分类号： C07H19/04
CPC分类号： C12Q1/6874 , C07H17/02 , C07H19/10 , C07H19/20 , C12Q1/6806
摘要： This disclosure provides systems and methods for attaching nano pore-detectable tags to nucleotides. The disclosure also provides methods for sequencing nucleic acids using the disdosed tagged nudeotides. Provided herein are nudeotides with attached tags and methods for attaching tags to nudeotides. The tags can be attached by chemical reactions, such as "dick chemistry". In an aspect, the present disdosure provides a tagged nudeotide, comprising: (a) a poly-phosphate moiety having a terminal phosphate; and (b) a tag covalently coupled to the terminal phosphate of the nudeotide by a triazole, a 1,2-diazine, a disulfide, a secondary amine, a hydrazone, a thio-acetamide, or a maleimide-thioadduct.
摘要翻译：本公开提供了将纳米孔可检测标签附着于核苷酸的系统和方法。本公开还提供了使用剔除的标记的核苷酸对核酸进行测序的方法。本文提供了具有附加标签的核苷酸和将标签附着到裸核苷酸的方法。标签可以通过化学反应来附着，例如“dick chemistry”。在一方面，本发明提供了一种标记的核苷酸，其包含：（a）具有末端磷酸酯的聚磷酸酯部分; 和（b）通过三唑，1,2-二嗪，二硫化物，仲胺，腙，硫代乙酰胺或马来酰亚胺 - 硫代加成酸共价偶联到核苷酸的末端磷酸酯上的标签。

3. 发明申请

WO2013188841A1 CHIP SET-UP AND HIGH-ACCURACY NUCLEIC ACID SEQUENCING 审中-公开
标题翻译：芯片设置和高精度核酸序列
公开(公告)号：WO2013188841A1
公开(公告)日：2013-12-19
申请号：PCT/US2013/046012
申请日：2013-06-14
申请人： GENIA TECHNOLOGIES, INC. , DAVIS, Randall , CHEN, Roger , BIBILLO, Arkadiusz , DEIERLING, Kevin
发明人： DAVIS, Randall , CHEN, Roger , BIBILLO, Arkadiusz , DEIERLING, Kevin
IPC分类号： C12Q1/68 , G01N33/53 , G01N33/48
CPC分类号： G01N27/44791 , C12Q1/6869 , G01N33/48721 , C12Q2565/631
摘要： The present disclosure provides devices, systems and methods for sequencing nucleic acid molecules. Nucleic acid molecules can be sequenced with a high accuracy (e.g., greater than 97% in a single pass) using a chip comprising an array of independently addressable nanopore sensors at a density of at least about 500 sites per 1 mm. An individual nanopore sensor can include a nanopore in a membrane that is adjacent or in proximity to a sensing electrode.
摘要翻译：本公开提供了用于测序核酸分子的装置，系统和方法。使用包含独立可寻址的纳米孔传感器阵列的芯片，以每1mm至少约500个位点的密度，可以以高精度（例如，单次通过中大于97％）对核酸分子进行测序。单个纳米孔传感器可以包括邻近或接近检测电极的膜中的纳米孔。

4. 发明申请

WO2013123450A1 METHODS FOR CREATING BILAYERS FOR USE WITH NANOPORE SENSORS 审中-公开
标题翻译：用NANOPORE传感器制造双层膜的方法
公开(公告)号：WO2013123450A1
公开(公告)日：2013-08-22
申请号：PCT/US2013/026514
申请日：2013-02-15
申请人： GENIA TECHNOLOGIES, INC , DAVIS, Randall , CHEN, Roger
发明人： DAVIS, Randall , CHEN, Roger
IPC分类号： C12Q1/68 , C12N15/11
CPC分类号： C12Q1/6869 , C12N15/111 , C12N2310/11 , C12N2310/16 , C12N2310/3517 , C12N2320/10 , C12N2320/32 , G01N33/48721 , G01N33/5432 , C12Q2565/607 , C12Q2565/631
摘要： The present disclosure provides biochips and methods for making biochips. A biochip can comprise a nanopore in a membrane (e.g., lipid bilayer) adjacent or in proximity to an electrode. Methods are described for forming the membrane and inserting the nanopore into the membrane. The biochips and methods can be used for nucleic acid (e.g., DNA) sequencing. The present disclosure also describes methods for detecting, sorting, and binning molecules (e.g., proteins) using biochips.
摘要翻译：本公开提供了用于制造生物芯片的生物芯片和方法。生物芯片可以包括邻近或接近电极的膜（例如脂质双层）中的纳米孔。描述了形成膜并将纳米孔插入膜中的方法。生物芯片和方法可用于核酸（例如DNA）测序。本公开还描述了使用生物芯片检测，分选和分类分子（例如蛋白质）的方法。

5. 发明申请

WO2013109970A1 NANOPORE BASED MOLECULAR DETECTION AND SEQUENCING 审中-公开
标题翻译：基于纳诺酮的分子检测和测序
公开(公告)号：WO2013109970A1
公开(公告)日：2013-07-25
申请号：PCT/US2013/022273
申请日：2013-01-18
申请人： GENIA TECHNOLOGIES, INC. , DAVIS, Randall , CHEN, Roger
发明人： DAVIS, Randall , CHEN, Roger
IPC分类号： G01N27/26 , C12Q1/68 , G01N27/414
CPC分类号： C12Q1/6869 , C12Q1/6874 , G01N33/48721 , C12Q2521/101 , C12Q2565/607
摘要： This disclosure provides systems and methods for molecular identification and polymer (e.g., nucleic acid) sequencing using nanopores. The polymer may be passed through or in proximity to the nanopore and various subunits of the polymer may affect the current flowing through the nanopore. The various subunits may be identified by measuring the current at a plurality of voltages applied across the nanopore and/or membrane. In some cases, the polymerization of tagged nucleotides presents tag molecules to the nanopore that can be identified by measuring the current at a plurality of voltages applied across the nanopore and/or membrane. Also provided herein are systems and methods for sequencing both the sense and anti-sense strand of a double stranded nucleic acid molecule with a nanopore and methods for using ribonucleic acid (RNA) speed bump molecules to slow the passage of a nucleic acid molecule through or in proximity to a nanopore.
摘要翻译：本公开提供用于分子鉴定和使用纳米孔的聚合物（例如，核酸）测序的系统和方法。聚合物可以通过纳米孔或靠近纳米孔，聚合物的各种亚基可能影响流过纳米孔的电流。可以通过测量跨过纳米孔和/或膜施加的多个电压的电流来识别各种亚基。在一些情况下，标记的核苷酸的聚合会向纳米孔呈现标签分子，其可以通过测量施加在纳米孔和/或膜上的多个电压的电流来鉴定。本文还提供了用纳米孔对双链核酸分子的有义链和反义链进行测序的系统和方法，以及使用核糖核酸（RNA）速度突变分子来减缓核酸分子通过的方法或靠近纳米孔。

6. 发明申请

WO2013067208A1 SYSTEMS AND METHODS FOR DETERMINING GENETIC DATA 审中-公开
标题翻译：用于确定遗传数据的系统和方法
公开(公告)号：WO2013067208A1
公开(公告)日：2013-05-10
申请号：PCT/US2012/063099
申请日：2012-11-01
申请人： GENIA TECHNOLOGIES, INC. , LIFE TECHNOLOGIES CORPORATION , GEISER, Timothy , DAVIS, Randall , CHEN, Roger
发明人： GEISER, Timothy , DAVIS, Randall , CHEN, Roger
IPC分类号： C12Q1/68 , B82Y10/00
CPC分类号： C12Q1/6874 , B82Y15/00 , C12Q1/6869 , C12Q2525/101 , C12Q2537/163 , C12Q2565/607 , C12Q2565/631 , C12Q2525/113 , C12Q2525/107
摘要： Systems and methods of polynucleotide sequencing are provided. Systems and methods optimize control, speed, movement, and/or translocation of a sample (e.g., a polynucleotide) within, through, or at least partially through a nanopore or a type of protein or mutant protein in order to accumulate sufficient time and current blocking information to identify contiguous nucleotides or plurality of nucleotides in a single-stranded area of a polynucleotide.
摘要翻译：提供了多核苷酸测序的系统和方法。系统和方法通过纳米孔或一种蛋白质或突变蛋白优化样品（例如，多核苷酸）的控制，速度，运动和/或易位，以便积累足够的时间和电流阻断信息以鉴定多核苷酸的单链区域中的连续核苷酸或多个核苷酸。

7. 发明申请

WO2012102856A1 TEMPERATURE REGULATION OF MEASUREMENT ARRAYS 审中-公开
标题翻译：测量阵列的温度调节
公开(公告)号：WO2012102856A1
公开(公告)日：2012-08-02
申请号：PCT/US2012/020827
申请日：2012-01-10
申请人： GENIA TECHNOLOGIES, INC. , CHEN, Roger
发明人： CHEN, Roger
IPC分类号： C12M1/38
CPC分类号： B01J19/0046 , B01J2219/00495 , B01J2219/00698 , G05D23/1919 , G05D23/2034 , G05D23/2037
摘要： A system for regulating a temperature of a measurement array is disclosed. The system includes a measurement array including a plurality of sensors, wherein the plurality of sensors are integrated onto an integrated circuit die. The system includes a thermal sensor integrated onto the integrated circuit die, wherein the thermal sensor senses a temperature associated with the plurality of sensors. The system further includes a heat pump coupled to the integrated circuit die, wherein the heat pump is controlled by a feedback control circuit including the thermal sensor.
摘要翻译：公开了一种用于调节测量阵列的温度的系统。该系统包括包括多个传感器的测量阵列，其中多个传感器集成在集成电路管芯上。该系统包括集成在集成电路管芯上的热传感器，其中热传感器感测与多个传感器相关联的温度。该系统还包括耦合到集成电路管芯的热泵，其中热泵由包括热传感器的反馈控制电路控制。

8. 发明申请

WO2012088341A2 NANOPORE-BASED SINGLE DNA MOLECULE CHARACTERIZATION, IDENTIFICATION AND ISOLATION USING SPEED BUMPS 审中-公开
标题翻译：基于纳米级的单分子DNA分子表征，鉴定和分离使用速度波
公开(公告)号：WO2012088341A2
公开(公告)日：2012-06-28
申请号：PCT/US2011/066632
申请日：2011-12-21
申请人： GENIA TECHNOLOGIES, INC. , DAVIS, Randy , CHEN, Roger
发明人： DAVIS, Randy , CHEN, Roger
IPC分类号： C12Q1/68 , G01N27/26 , B82Y10/00
CPC分类号： C12Q1/6869 , B82Y10/00 , B82Y15/00 , C12Q1/6816 , C12Q1/6825 , G01N27/447 , G01N33/48721 , Y10S977/924 , C12Q2525/119 , C12Q2525/121 , C12Q2525/204 , C12Q2527/101 , C12Q2563/149 , C12Q2565/631
摘要： The present invention relates to a method of using nanopores to obtain sequence information of sample DNAs in ss test DNAs. The method comprises using speed bumps to stall the ss test DNAs in the nanopores at random positions of the ss test DNAs to obtain sequence information of each and every nucleotides of the sample DNAs, and to construct the whole sequences of the sample DNAs. The present invention also relates to identification and/or isolation of test DNAs having desired sequence(s) using nanopore detectors facilitated by speed bump.
摘要翻译：本发明涉及使用纳米孔以获得ss试验DNA中样品DNA序列信息的方法。该方法包括使用速度凸块来阻断ss测试DNA的随机位置的纳米孔中的ss测试DNA，以获得样品DNA的每个核苷酸的序列信息，并构建样品DNA的整个序列。本发明还涉及使用由缓冲器促进的纳米孔检测器来鉴定和/或分离具有所需序列的测试DNA。

9. 发明申请

WO2012088339A2 NANOPORE-BASED SINGLE DNA MOLECULE CHARACTERIZATION USING SPEED BUMPS 审中-公开
标题翻译：基于纳米级的单分子DNA分子表征使用速度波峰
公开(公告)号：WO2012088339A2
公开(公告)日：2012-06-28
申请号：PCT/US2011/066627
申请日：2011-12-21
申请人： GENIA TECHNOLOGIES, INC. , DAVIS, Randy , CHEN, Roger
发明人： DAVIS, Randy , CHEN, Roger
IPC分类号： C12Q1/68 , G01N27/26 , B82Y10/00
CPC分类号： C12Q1/6869 , B82Y10/00 , B82Y15/00 , C12Q1/6816 , C12Q1/6825 , G01N27/447 , G01N33/48721 , Y10S977/924 , C12Q2525/119 , C12Q2525/121 , C12Q2525/204 , C12Q2527/101 , C12Q2563/149 , C12Q2565/631
摘要： The present invention relates to a method of using nanopore to obtain sequence information of an unknown structure (unknown DNA) in a ss test DNA. The method comprises using speed bump to stall the ss test DNA in the nanopore at random positions of the ss test DNA to obtain sequence information of each and every nucleotides of the unknown DNA, and to construct the whole sequence of the unknown DNA. The present invention also relates to a novel method of trapping a ss test DNA in a nanopore using two bulky structures formed under different conditions (e.g. different temperature), and the bulky structures are able to keep the ss test DNA trapped in a nanopore at a working temperature.
摘要翻译：本发明涉及使用纳米孔以获得ss检验DNA中未知结构（未知DNA）的序列信息的方法。该方法包括使用速度碰撞来阻止ss测试DNA随机位置的纳米孔中的ss测试DNA，以获得未知DNA的每个核苷酸的序列信息，并构建未知DNA的整个序列。本发明还涉及使用在不同条件（例如不同温度）下形成的两个庞大结构在纳米孔中捕获ss测试DNA的新方法，并且大体积结构能够将ss测试DNA捕获在纳米孔中工作温度

10. 发明申请

WO2012102797A2 SYSTEM FOR COMMUNICATING INFORMATION FROM AN ARRAY OF SENSORS 审中-公开
标题翻译：从传感器阵列传播信息的系统
公开(公告)号：WO2012102797A2
公开(公告)日：2012-08-02
申请号：PCT/US2011/064490
申请日：2011-12-12
申请人： GENIA TECHNOLOGIES, INC. , CHEN, Roger
发明人： CHEN, Roger
IPC分类号： G08C15/06
CPC分类号： G01N27/3278 , C12Q1/6874 , G01N27/44791 , G01N33/48721
摘要： A system for communicating information from an array of sensors is disclosed. The system comprises a sensor array that includes a plurality of sensors, wherein each sensor senses a physical property of a material that is in communication with the sensor. The system further comprises signal processing circuitry associated with each sensor that integrates the output of the sensor over time and compares the integrated output to a threshold. The system further comprises a communication network coupled to the signal processing circuitry that outputs information indicating that the integrated output corresponding to a given sensor has reached the threshold.
摘要翻译：公开了一种用于从传感器阵列传送信息的系统。该系统包括传感器阵列，其包括多个传感器，其中每个传感器感测与传感器通信的材料的物理性质。该系统还包括与每个传感器相关联的信号处理电路，其将传感器的输出随时间积分，并将积分输出与阈值进行比较。该系统还包括耦合到信号处理电路的通信网络，其输出指示对应于给定传感器的集成输出已经达到阈值的信息。

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